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Gene information
General information
TC013522 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8440046..8443673
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013522
    iBeetle screen iB_05443

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013522 RA
      MDRGKFSLL LLDPGEIYF EDFSAVLIP PDITPKTFD 
      SKKQDGRLK MCSKSLVFD PKDIYKPII KIPLKNCVV 
      IEQWKGSAK YLTNNNVLT VNCREYIEM LEGNIVAPY 
      KFREATNFL FQLNYANIM NCLPQICQL HRASTLPAA 
      EQADMIATI VHSRHARVS FDPLWLDLY EKVVLETEA 
      DKVTPLVVN PGRLLLSTS RLFFQPYNN VESVPVIKI 
      ELNSIKRMV KRRFLLRHI GLEIYCSES SSNPHIYLS 
      FRSQQKRDH LYDNLLNQP VLKLEELDQ DVMTLQWQN 
      GIISNYDYL QYINSLGDR TINDLTQYP IFPWIISNY 
      TDPELDFKD PKNYRDLSK PVGALNEVR LRRLLERYD 
      EMSHPKFLY GSHYSTPGF VLFYLARLY PHYVLCLQS 
      GRFDHPDRM FNSVADVYK NCQTNMSDF KELIPEFYD 
      VSQEGNFLV NNMGINFGY RHNGLKVAD VELPPWADS 
      PQDFVRKLR DALESDVVS QGLHQWIDL IFGFKQQGE 
      EAVKANNLF YHLCYEGNV DLDKIQDLN QRHALEVQI 
      MEFGQIPKQ VFKVPHPAR KIGLPLLTE PHQVNLTET 
      EDLWKNITS LEVVSTFNT HKNTVSHLF ISDDGSRVT 
      SVGHDSKLK VFSLEQSRQ TRSANIGNM PLSSCIQMP 
      NVNVLVIGS WDNQILLYD LDYGKVTES VLAHEDAIT 
      CMCWGRKSN ILVSGSGDC TVKIWKGLN NNGIIKPIQ 
      CLQKQIDHN SHVNCLDFD NDNEHLAVG TEDGEVYIW 
      KTGDFTLYK KYTIHSSSI TAINYSPDG LKLALGAKN 
      KTLQIVDVG TGLPVFTKT LKSAISCLK WVGFLLVLG 
      CDDGSLFIW DIVEVRLLY EVSGAHSGA IKTVDISPE 
      HAIIVTGSE DRLIKVWKP K
    CDS sequence
    • >TC013522 RA
      atggatcgg gggaaattt tcgttattg ttattggat 
      cccggtgag atctatttt gaggacttc agcgcagtt 
      ttgatacct ccggacata acgccgaaa acgttcgat 
      tcaaagaaa caggatggc aggctcaaa atgtgttca 
      aaatcttta gtttttgac ccaaaagat atctacaaa 
      ccaattatc aaaataccg ttaaaaaac tgtgttgtg 
      atcgagcaa tggaaaggt agcgcaaag tatttaaca 
      aataacaac gtcctaact gtaaactgt cgggagtac 
      attgaaatg ttagaagga aatattgtt gccccctat 
      aaattccgc gaggctact aatttcttg tttcaatta 
      aactacgcc aacattatg aactgtttg ccccaaatt 
      tgtcagtta catagggcc agcacttta ccggccgct 
      gaacaagct gacatgatc gcaactatc gttcattca 
      cgacatgct cgggttagt tttgaccct ttgtggtta 
      gatttatat gaaaaggtt gttttggag actgaggca 
      gataaagtt actcctctt gttgtaaac cctggtaga 
      cttttatta tcaacatca cgattgttt ttccaaccc 
      tataataat gtagaaagc gttccggtt attaaaatt 
      gagctgaac agcatcaag aggatggtg aaaaggcgg 
      ttcttgttg aggcacata ggcttggaa atttattgt 
      agcgaaagt agtagtaac cctcacatt tatctatcg 
      tttcgaagt cagcaaaaa cgcgatcat ttatacgat 
      aatttattg aaccaaccg gtcttaaaa ttggaggag 
      ctagaccaa gatgttatg actctccaa tggcaaaat 
      ggtattata tcaaattat gattacctc caatatatc 
      aatagctta ggcgaccgt acaatcaac gatttaacc 
      cagtacccc attttccct tggattatt tcaaactac 
      accgaccca gaattagac ttcaaagac ccaaaaaat 
      tacagagac ttgagtaaa ccagtcggt gcattaaac 
      gaggtccgt ttacgccga cttctggaa cgatatgac 
      gaaatgtcg catccgaaa tttttgtat ggatcgcac 
      tactcaacc cctggcttt gttctcttt tatttagca 
      cgtctttac ccccattac gtcctgtgc ctacaaagt 
      gggcgcttt gaccatcca gaccgaatg ttcaattca 
      gtggcagat gtttacaaa aattgccaa accaacatg 
      tccgacttc aaagaatta atacccgag ttttacgac 
      gtgagccaa gagggaaat ttccttgtt aataacatg 
      gggatcaat ttcgggtat cgccacaac gggctcaaa 
      gtcgctgac gttgaattg cccccatgg gccgacagc 
      ccccaggac ttcgttcgc aaattgagg gacgccttg 
      gagagtgat gtggtgtcg caggggctc caccagtgg 
      attgacctg atttttggg tttaagcag caaggggaa 
      gaagcggtg aaggctaat aatttgttt tatcatttg 
      tgttacgag ggaaatgtc gatttggat aaaatacaa 
      gatttgaat caacgacac gctttggaa gtgcaaata 
      atggagttt gggcaaatt cccaaacaa gtgttcaaa 
      gtgccgcat ccggcgcgc aaaataggg cttcctctg 
      ttgaccgaa ccgcatcag gttaatcta acagaaaca 
      gaagatctt tggaaaaat atcaccagt ttggaagtt 
      gtttccaca tttaacacc cacaagaac acggtgagt 
      caccttttc atcagcgat gatgggtct cgtgtgact 
      tccgtcggt catgattcc aagctaaag gtgttttct 
      ttggaacaa agtcgccag actagaagc gcaaacatt 
      ggaaatatg cctttaagt agctgcatt caaatgcct 
      aatgtcaat gttctcgtt atcggcagt tgggacaat 
      caaattttg ttgtacgac ctggactat gggaaagtc 
      accgaaagc gtattagcc cacgaagac gcgatcact 
      tgcatgtgc tggggcaga aagagtaat attttggtg 
      tccggcagt ggcgactgc actgttaaa atatggaaa 
      ggtttgaat aacaacgga ataatcaag cccattcaa 
      tgtttacag aaacaaatc gaccacaat tcgcacgtt 
      aactgcttg gacttcgac aatgataac gaacacctc 
      gccgttggc acggaagac ggggaagtg tatatttgg 
      aaaacgggc gatttcacc ctttacaaa aaatacaca 
      atacattct tcgagcatc acagcgatt aattacagt 
      cccgacggc ttgaaattg gccctgggt gccaaaaac 
      aaaacttta caaattgta gatgtggga actgggctt 
      cctgttttc acgaaaact ttgaaatcg gccatttcg 
      tgtttgaaa tgggtgggg tttttactg gttttaggc 
      tgtgacgat ggcagtttg tttatttgg gacattgtg 
      gaagttagg ttgctttac gaagtcagt ggggcccac 
      tctggagca ataaaaact gtcgatatt tcgcccgag 
      cacgctatc attgttacc gggagtgag gatcgcctt 
      atcaaagtg tggaaacca aaataa
    mRNA sequence
    • >TC013522 RA
      tgacagctg taaacaaag tttgaggtt attagcatt 
      atttattat gtagaaaaa gtggtaagc agaattctt 
      tagataaat gcaacttta taatatgtg caaaatgat 
      gcaactgta gttatctcg gcaactttt acgtgtgac 
      aatggatcg ggggaaatt ttcgttatt gttattgga 
      tcccggtga gatctattt tgaggactt cagcgcagt 
      tttgatacc tccggacat aacgccgaa aacgttcga 
      ttcaaagaa acaggatgg caggctcaa aatgtgttc 
      aaaatcttt agtttttga cccaaaaga tatctacaa 
      accaattat caaaatacc gttaaaaaa ctgtgttgt 
      gatcgagca atggaaagg tagcgcaaa gtatttaac 
      aaataacaa cgtcctaac tgtaaactg tcgggagta 
      cattgaaat gttagaagg aaatattgt tgcccccta 
      taaattccg cgaggctac taatttctt gtttcaatt 
      aaactacgc caacattat gaactgttt gccccaaat 
      ttgtcagtt acatagggc cagcacttt accggccgc 
      tgaacaagc tgacatgat cgcaactat cgttcattc 
      acgacatgc tcgggttag ttttgaccc tttgtggtt 
      agatttata tgaaaaggt tgttttgga gactgaggc 
      agataaagt tactcctct tgttgtaaa ccctggtag 
      acttttatt atcaacatc acgattgtt tttccaacc 
      ctataataa tgtagaaag cgttccggt tattaaaat 
      tgagctgaa cagcatcaa gaggatggt gaaaaggcg 
      gttcttgtt gaggcacat aggcttgga aatttattg 
      tagcgaaag tagtagtaa ccctcacat ttatctatc 
      gtttcgaag tcagcaaaa acgcgatca tttatacga 
      taatttatt gaaccaacc ggtcttaaa attggagga 
      gctagacca agatgttat gactctcca atggcaaaa 
      tggtattat atcaaatta tgattacct ccaatatat 
      caatagctt aggcgaccg tacaatcaa cgatttaac 
      ccagtaccc cattttccc ttggattat ttcaaacta 
      caccgaccc agaattaga cttcaaaga cccaaaaaa 
      ttacagaga cttgagtaa accagtcgg tgcattaaa 
      cgaggtccg tttacgccg acttctgga acgatatga 
      cgaaatgtc gcatccgaa atttttgta tggatcgca 
      ctactcaac ccctggctt tgttctctt ttatttagc 
      acgtcttta cccccatta cgtcctgtg cctacaaag 
      tgggcgctt tgaccatcc agaccgaat gttcaattc 
      agtggcaga tgtttacaa aaattgcca aaccaacat 
      gtccgactt caaagaatt aatacccga gttttacga 
      cgtgagcca agagggaaa tttccttgt taataacat 
      ggggatcaa tttcgggta tcgccacaa cgggctcaa 
      agtcgctga cgttgaatt gcccccatg ggccgacag 
      cccccagga cttcgttcg caaattgag ggacgcctt 
      ggagagtga tgtggtgtc gcaggggct ccaccagtg 
      gattgacct gatttttgg gtttaagca gcaagggga 
      agaagcggt gaaggctaa taatttgtt ttatcattt 
      gtgttacga gggaaatgt cgatttgga taaaataca 
      agatttgaa tcaacgaca cgctttgga agtgcaaat 
      aatggagtt tgggcaaat tcccaaaca agtgttcaa 
      agtgccgca tccggcgcg caaaatagg gcttcctct 
      gttgaccga accgcatca ggttaatct aacagaaac 
      agaagatct ttggaaaaa tatcaccag tttggaagt 
      tgtttccac atttaacac ccacaagaa cacggtgag 
      tcacctttt catcagcga tgatgggtc tcgtgtgac 
      ttccgtcgg tcatgattc caagctaaa ggtgttttc 
      tttggaaca aagtcgcca gactagaag cgcaaacat 
      tggaaatat gcctttaag tagctgcat tcaaatgcc 
      taatgtcaa tgttctcgt tatcggcag ttgggacaa 
      tcaaatttt gttgtacga cctggacta tgggaaagt 
      caccgaaag cgtattagc ccacgaaga cgcgatcac 
      ttgcatgtg ctggggcag aaagagtaa tattttggt 
      gtccggcag tggcgactg cactgttaa aatatggaa 
      aggtttgaa taacaacgg aataatcaa gcccattca 
      atgtttaca gaaacaaat cgaccacaa ttcgcacgt 
      taactgctt ggacttcga caatgataa cgaacacct 
      cgccgttgg cacggaaga cggggaagt gtatatttg 
      gaaaacggg cgatttcac cctttacaa aaaatacac 
      aatacattc ttcgagcat cacagcgat taattacag 
      tcccgacgg cttgaaatt ggccctggg tgccaaaaa 
      caaaacttt acaaattgt agatgtggg aactgggct 
      tcctgtttt cacgaaaac tttgaaatc ggccatttc 
      gtgtttgaa atgggtggg gtttttact ggttttagg 
      ctgtgacga tggcagttt gtttatttg ggacattgt 
      ggaagttag gttgcttta cgaagtcag tggggccca 
      ctctggagc aataaaaac tgtcgatat ttcgcccga 
      gcacgctat cattgttac cgggagtga ggatcgcct 
      tatcaaagt gtggaaacc aaaataaaa caataattt 
      tacatagta ttttaactt aagtattgt gatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY