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Gene information
General information
TC033122
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013513,TC013511 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8615547..8650039
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033122
    iBeetle screen iB_05442

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     cuticle remnants without visible segmentation, differentiation or axis -

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 8 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033122 RA
      MSPPLPQPL IDSSSHAKL IADHTHYPP RPPRPRPGT 
APSCSAAGP RPRPVSLSV GTSCTGHVL TGVGSGDCD 
FPVFVMLLG AMPEESAAA GLLAPTAKT RATDFSIAA 
IMARGAVAP PDADARELP GDGAASRSS SPDMEDPLE 
DDDIEVDVE ECSDSESAR ASKPKLRQS PAISDCGSE 
PADLDRESP DIIQEKPIS KAKISCNCD ELLNVECHL 
ETKDLWDKF HDLGTEMII TKTGRRMFP TLRVSFTGI 
RPDQRYAVL LDIVPVDNK RYRYAYHRS SWLVAGKAD 
PPAPCRIYA HPDSPFSGE QLRKQVVSF EKVKLTNNE 
MDKNGQIVL NSMHRYQPR IHIVKWREH SGPITDLEQ 
EQYRTFIFP ESVFTAVTA YQNQLITKL KIDSNPFAK 
GFRDSSRLT DFDREPMDS LFMDQHFLR SSLRLYSGD 
SLDAENNNA GSLFSAAMM EKARAHIQM WGRASGGAP 
YNPSELHAF LLNSSPSQQ IYLGQRPSV PLGLTSQLW 
SQGGSSWQS PIHGGLPPG LLGPPPPHR PQMTPPPSS 
TPSSSGSPS PTSVSTSDL RLPKAVFPS SMHRFSPYA 
SPLPRQAPS LSPTSRSGH
    CDS sequence
    • >TC033122 RA
      atgtcgcca ccgctacca cagcctctg attgatagc 
tcaagtcac gcgaaactg atagcagac cacacacac 
taccctcca cgccccccg aggccccgc cccgggacc 
gccccctcg tgcagcgcc gccggaccg cggccccga 
ccagtcagt ctttcagtg ggtaccagt tgtactgga 
catgtgctg actggcgtg ggttctggt gactgtgac 
tttccagtg tttgtgatg cttctcggt gcgatgccc 
gaggaaagt gcagccgcg gggttgctc gcgcccacg 
gcgaagacg cgcgccacc gacttctcc atcgccgcg 
atcatggcc aggggggct gtcgcgccg ccggacgcc 
gacgccagg gaactgcca ggcgacggt gccgcttcg 
cgatccagc tcacccgac atggaggat cccttagag 
gacgatgat atagaagta gacgtggaa gagtgttcg 
gacagcgaa tcggcaagg gcttcgaaa ccgaaactt 
cgacaatct cccgcaatt tcggactgt ggctcagaa 
ccggccgat ttagaccgg gaatccccc gacataatc 
caagagaaa cccatttcg aaagcaaaa atatcgtgc 
aactgcgac gagttgctg aacgtcgaa tgccacctc 
gaaaccaaa gacctctgg gacaagttc cacgatctt 
ggcacggaa atgatcatc accaaaacc gggagacga 
atgttcccg actttgagg gtttccttc accggaatt 
cgcccggac cagcggtac gccgtcctg ctggacatc 
gttccggtt gacaacaaa cgctatcgg tacgcttac 
caccggtcc tcgtggctg gtggcgggc aaggccgat 
cctccggct ccctgcagg atatacgcg catccggac 
tcgcccttc tccggggag cagctacga aagcaagtc 
gtctccttc gagaaggtc aaactcacc aacaacgaa 
atggacaaa aacggacag atcgtcctg aactccatg 
caccggtac cagccccgg atccacatc gtcaagtgg 
cgggagcac tcgggcccg atcacggac ctggagcag 
gagcagtac agaacattc attttcccc gaatcggtt 
ttcacggca gttacggcc tatcaaaat caactcatc 
acgaaattg aaaatcgat tcgaaccca ttcgccaaa 
gggttcagg gattcgtcc aggctcacg gattttgac 
agagagcca atggacagc ctcttcatg gaccagcac 
ttcctgcgc tcatccctc cgcctctac tccggcgac 
tccctcgac gccgagaac aacaacgcc ggctcgctc 
ttctcggcc gcgatgatg gagaaggcc cgagcgcac 
atccagatg tggggccgc gcctcgggc ggcgcccct 
tacaacccc agtgagctg cacgcgttc ctcctcaac 
agctcccca agccagcaa atctacctg ggccagcgg 
ccctccgtt cccctgggc ttgacgagc cagctgtgg 
agccagggc ggctcgtcc tggcagtcg cccatccac 
ggcggcctg cccccgggc ctgctgggc ccgccgccg 
cctcaccgg ccccagatg accccgccg ccctcttcc 
acccccagt tcctcggga tcgcccagt cccacctcc 
gttagtact tccgatctg cggttaccc aaagctgtg 
ttcccctct tcgatgcac cgcttcagc ccctacgcg 
tcgcccctg ccccggcaa gccccctcg ctgagcccc 
acctcgcgg tccggacac tga
    mRNA sequence
    • >TC033122 RA
      cgcgggcag aagttttaa acgatctcg tttagttag 
atgcagatc tgagaaagt tcatcataa gcgtcgact 
atgtcgcca ccgctacca cagcctctg attgatagc 
tcaagtcac gcgaaactg atagcagac cacacacac 
taccctcca cgccccccg aggccccgc cccgggacc 
gccccctcg tgcagcgcc gccggaccg cggccccga 
ccagtcagt ctttcagtg ggtaccagt tgtactgga 
catgtgctg actggcgtg ggttctggt gactgtgac 
tttccagtg tttgtgatg cttctcggt gcgatgccc 
gaggaaagt gcagccgcg gggttgctc gcgcccacg 
gcgaagacg cgcgccacc gacttctcc atcgccgcg 
atcatggcc aggggggct gtcgcgccg ccggacgcc 
gacgccagg gaactgcca ggcgacggt gccgcttcg 
cgatccagc tcacccgac atggaggat cccttagag 
gacgatgat atagaagta gacgtggaa gagtgttcg 
gacagcgaa tcggcaagg gcttcgaaa ccgaaactt 
cgacaatct cccgcaatt tcggactgt ggctcagaa 
ccggccgat ttagaccgg gaatccccc gacataatc 
caagagaaa cccatttcg aaagcaaaa atatcgtgc 
aactgcgac gagttgctg aacgtcgaa tgccacctc 
gaaaccaaa gacctctgg gacaagttc cacgatctt 
ggcacggaa atgatcatc accaaaacc gggagacga 
atgttcccg actttgagg gtttccttc accggaatt 
cgcccggac cagcggtac gccgtcctg ctggacatc 
gttccggtt gacaacaaa cgctatcgg tacgcttac 
caccggtcc tcgtggctg gtggcgggc aaggccgat 
cctccggct ccctgcagg atatacgcg catccggac 
tcgcccttc tccggggag cagctacga aagcaagtc 
gtctccttc gagaaggtc aaactcacc aacaacgaa 
atggacaaa aacggacag atcgtcctg aactccatg 
caccggtac cagccccgg atccacatc gtcaagtgg 
cgggagcac tcgggcccg atcacggac ctggagcag 
gagcagtac agaacattc attttcccc gaatcggtt 
ttcacggca gttacggcc tatcaaaat caactcatc 
acgaaattg aaaatcgat tcgaaccca ttcgccaaa 
gggttcagg gattcgtcc aggctcacg gattttgac 
agagagcca atggacagc ctcttcatg gaccagcac 
ttcctgcgc tcatccctc cgcctctac tccggcgac 
tccctcgac gccgagaac aacaacgcc ggctcgctc 
ttctcggcc gcgatgatg gagaaggcc cgagcgcac 
atccagatg tggggccgc gcctcgggc ggcgcccct 
tacaacccc agtgagctg cacgcgttc ctcctcaac 
agctcccca agccagcaa atctacctg ggccagcgg 
ccctccgtt cccctgggc ttgacgagc cagctgtgg 
agccagggc ggctcgtcc tggcagtcg cccatccac 
ggcggcctg cccccgggc ctgctgggc ccgccgccg 
cctcaccgg ccccagatg accccgccg ccctcttcc 
acccccagt tcctcggga tcgcccagt cccacctcc 
gttagtact tccgatctg cggttaccc aaagctgtg 
ttcccctct tcgatgcac cgcttcagc ccctacgcg 
tcgcccctg ccccggcaa gccccctcg ctgagcccc 
acctcgcgg tccggacac tgatgaaga gtcgctccg 
agccgaaac tataccaaa gagtctttc cggaagacg 
ctaattgtg agattatat ccttgtaaa taaactgtc 
gttgaaatt atttatttg tgtaaaata gggagtcct 
ggtagagtc actgatttt ttttattac ttgtaaagt 
gtacgtgta attatattt tgtacaaca gatagcagt 
attaatgta cataatcat cagtgtagt acttacgtt 
ctcctaatt gtgagaact gaaacgttc cttttgttg 
ttggttcta cggcacgct cgaacattt attttttta 
cattaccgg cattccata tcagataaa gtcagtggt 
tcagtttta aatcgtttc aaatgttgc tacagaacc 
aaatttttt ataagaaat agtttatgt acctaccgt 
aaaatgtat attttatga aaatatcct tcactgtca 
attatttac ttagttttt cattgtatt acactacgt 
taaaaagga atttgttgt tgtgtatat ataatatgt 
ggattgtaa attaaataa acaattatt gaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY