Status
Please wait ...
Gene information
General information
TC033122
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013513,TC013511
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8615547..8650039
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033122
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05442

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation, differentiation or axis -

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 8 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033122 RA
      MSPPLPQPL IDSSSHAKL IADHTHYPP RPPRPRPGT 
      APSCSAAGP RPRPVSLSV GTSCTGHVL TGVGSGDCD 
      FPVFVMLLG AMPEESAAA GLLAPTAKT RATDFSIAA 
      IMARGAVAP PDADARELP GDGAASRSS SPDMEDPLE 
      DDDIEVDVE ECSDSESAR ASKPKLRQS PAISDCGSE 
      PADLDRESP DIIQEKPIS KAKISCNCD ELLNVECHL 
      ETKDLWDKF HDLGTEMII TKTGRRMFP TLRVSFTGI 
      RPDQRYAVL LDIVPVDNK RYRYAYHRS SWLVAGKAD 
      PPAPCRIYA HPDSPFSGE QLRKQVVSF EKVKLTNNE 
      MDKNGQIVL NSMHRYQPR IHIVKWREH SGPITDLEQ 
      EQYRTFIFP ESVFTAVTA YQNQLITKL KIDSNPFAK 
      GFRDSSRLT DFDREPMDS LFMDQHFLR SSLRLYSGD 
      SLDAENNNA GSLFSAAMM EKARAHIQM WGRASGGAP 
      YNPSELHAF LLNSSPSQQ IYLGQRPSV PLGLTSQLW 
      SQGGSSWQS PIHGGLPPG LLGPPPPHR PQMTPPPSS 
      TPSSSGSPS PTSVSTSDL RLPKAVFPS SMHRFSPYA 
      SPLPRQAPS LSPTSRSGH
    CDS sequence
    • >TC033122 RA
      atgtcgcca ccgctacca cagcctctg attgatagc 
      tcaagtcac gcgaaactg atagcagac cacacacac 
      taccctcca cgccccccg aggccccgc cccgggacc 
      gccccctcg tgcagcgcc gccggaccg cggccccga 
      ccagtcagt ctttcagtg ggtaccagt tgtactgga 
      catgtgctg actggcgtg ggttctggt gactgtgac 
      tttccagtg tttgtgatg cttctcggt gcgatgccc 
      gaggaaagt gcagccgcg gggttgctc gcgcccacg 
      gcgaagacg cgcgccacc gacttctcc atcgccgcg 
      atcatggcc aggggggct gtcgcgccg ccggacgcc 
      gacgccagg gaactgcca ggcgacggt gccgcttcg 
      cgatccagc tcacccgac atggaggat cccttagag 
      gacgatgat atagaagta gacgtggaa gagtgttcg 
      gacagcgaa tcggcaagg gcttcgaaa ccgaaactt 
      cgacaatct cccgcaatt tcggactgt ggctcagaa 
      ccggccgat ttagaccgg gaatccccc gacataatc 
      caagagaaa cccatttcg aaagcaaaa atatcgtgc 
      aactgcgac gagttgctg aacgtcgaa tgccacctc 
      gaaaccaaa gacctctgg gacaagttc cacgatctt 
      ggcacggaa atgatcatc accaaaacc gggagacga 
      atgttcccg actttgagg gtttccttc accggaatt 
      cgcccggac cagcggtac gccgtcctg ctggacatc 
      gttccggtt gacaacaaa cgctatcgg tacgcttac 
      caccggtcc tcgtggctg gtggcgggc aaggccgat 
      cctccggct ccctgcagg atatacgcg catccggac 
      tcgcccttc tccggggag cagctacga aagcaagtc 
      gtctccttc gagaaggtc aaactcacc aacaacgaa 
      atggacaaa aacggacag atcgtcctg aactccatg 
      caccggtac cagccccgg atccacatc gtcaagtgg 
      cgggagcac tcgggcccg atcacggac ctggagcag 
      gagcagtac agaacattc attttcccc gaatcggtt 
      ttcacggca gttacggcc tatcaaaat caactcatc 
      acgaaattg aaaatcgat tcgaaccca ttcgccaaa 
      gggttcagg gattcgtcc aggctcacg gattttgac 
      agagagcca atggacagc ctcttcatg gaccagcac 
      ttcctgcgc tcatccctc cgcctctac tccggcgac 
      tccctcgac gccgagaac aacaacgcc ggctcgctc 
      ttctcggcc gcgatgatg gagaaggcc cgagcgcac 
      atccagatg tggggccgc gcctcgggc ggcgcccct 
      tacaacccc agtgagctg cacgcgttc ctcctcaac 
      agctcccca agccagcaa atctacctg ggccagcgg 
      ccctccgtt cccctgggc ttgacgagc cagctgtgg 
      agccagggc ggctcgtcc tggcagtcg cccatccac 
      ggcggcctg cccccgggc ctgctgggc ccgccgccg 
      cctcaccgg ccccagatg accccgccg ccctcttcc 
      acccccagt tcctcggga tcgcccagt cccacctcc 
      gttagtact tccgatctg cggttaccc aaagctgtg 
      ttcccctct tcgatgcac cgcttcagc ccctacgcg 
      tcgcccctg ccccggcaa gccccctcg ctgagcccc 
      acctcgcgg tccggacac tga
    mRNA sequence
    • >TC033122 RA
      cgcgggcag aagttttaa acgatctcg tttagttag 
      atgcagatc tgagaaagt tcatcataa gcgtcgact 
      atgtcgcca ccgctacca cagcctctg attgatagc 
      tcaagtcac gcgaaactg atagcagac cacacacac 
      taccctcca cgccccccg aggccccgc cccgggacc 
      gccccctcg tgcagcgcc gccggaccg cggccccga 
      ccagtcagt ctttcagtg ggtaccagt tgtactgga 
      catgtgctg actggcgtg ggttctggt gactgtgac 
      tttccagtg tttgtgatg cttctcggt gcgatgccc 
      gaggaaagt gcagccgcg gggttgctc gcgcccacg 
      gcgaagacg cgcgccacc gacttctcc atcgccgcg 
      atcatggcc aggggggct gtcgcgccg ccggacgcc 
      gacgccagg gaactgcca ggcgacggt gccgcttcg 
      cgatccagc tcacccgac atggaggat cccttagag 
      gacgatgat atagaagta gacgtggaa gagtgttcg 
      gacagcgaa tcggcaagg gcttcgaaa ccgaaactt 
      cgacaatct cccgcaatt tcggactgt ggctcagaa 
      ccggccgat ttagaccgg gaatccccc gacataatc 
      caagagaaa cccatttcg aaagcaaaa atatcgtgc 
      aactgcgac gagttgctg aacgtcgaa tgccacctc 
      gaaaccaaa gacctctgg gacaagttc cacgatctt 
      ggcacggaa atgatcatc accaaaacc gggagacga 
      atgttcccg actttgagg gtttccttc accggaatt 
      cgcccggac cagcggtac gccgtcctg ctggacatc 
      gttccggtt gacaacaaa cgctatcgg tacgcttac 
      caccggtcc tcgtggctg gtggcgggc aaggccgat 
      cctccggct ccctgcagg atatacgcg catccggac 
      tcgcccttc tccggggag cagctacga aagcaagtc 
      gtctccttc gagaaggtc aaactcacc aacaacgaa 
      atggacaaa aacggacag atcgtcctg aactccatg 
      caccggtac cagccccgg atccacatc gtcaagtgg 
      cgggagcac tcgggcccg atcacggac ctggagcag 
      gagcagtac agaacattc attttcccc gaatcggtt 
      ttcacggca gttacggcc tatcaaaat caactcatc 
      acgaaattg aaaatcgat tcgaaccca ttcgccaaa 
      gggttcagg gattcgtcc aggctcacg gattttgac 
      agagagcca atggacagc ctcttcatg gaccagcac 
      ttcctgcgc tcatccctc cgcctctac tccggcgac 
      tccctcgac gccgagaac aacaacgcc ggctcgctc 
      ttctcggcc gcgatgatg gagaaggcc cgagcgcac 
      atccagatg tggggccgc gcctcgggc ggcgcccct 
      tacaacccc agtgagctg cacgcgttc ctcctcaac 
      agctcccca agccagcaa atctacctg ggccagcgg 
      ccctccgtt cccctgggc ttgacgagc cagctgtgg 
      agccagggc ggctcgtcc tggcagtcg cccatccac 
      ggcggcctg cccccgggc ctgctgggc ccgccgccg 
      cctcaccgg ccccagatg accccgccg ccctcttcc 
      acccccagt tcctcggga tcgcccagt cccacctcc 
      gttagtact tccgatctg cggttaccc aaagctgtg 
      ttcccctct tcgatgcac cgcttcagc ccctacgcg 
      tcgcccctg ccccggcaa gccccctcg ctgagcccc 
      acctcgcgg tccggacac tgatgaaga gtcgctccg 
      agccgaaac tataccaaa gagtctttc cggaagacg 
      ctaattgtg agattatat ccttgtaaa taaactgtc 
      gttgaaatt atttatttg tgtaaaata gggagtcct 
      ggtagagtc actgatttt ttttattac ttgtaaagt 
      gtacgtgta attatattt tgtacaaca gatagcagt 
      attaatgta cataatcat cagtgtagt acttacgtt 
      ctcctaatt gtgagaact gaaacgttc cttttgttg 
      ttggttcta cggcacgct cgaacattt attttttta 
      cattaccgg cattccata tcagataaa gtcagtggt 
      tcagtttta aatcgtttc aaatgttgc tacagaacc 
      aaatttttt ataagaaat agtttatgt acctaccgt 
      aaaatgtat attttatga aaatatcct tcactgtca 
      attatttac ttagttttt cattgtatt acactacgt 
      taaaaagga atttgttgt tgtgtatat ataatatgt 
      ggattgtaa attaaataa acaattatt gaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY