Status
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Gene information
General information
TC013504 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8698741..8717995
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013504
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05441

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva lateral bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013504 RA
      MNEAVVLLL FLTSVHAAN DNEDCKIRF LSKPTAPSR 
      LNLNVTAVT SSIFANRAS DNFSDIARL TLTTHPEIL 
      GLVLKSPNE QILALNVAQ RLRIFSNKT VENEAFWTG 
      LGNQTQGWT PAFRVCHFL KGSWFYGFV ATKSNLTTA 
      LFLNLQLDQ CDDNLEEIF GKRHKCDQE TTYCVPQPE 
      ENGGYSCFC RQGFFHPDA SLSWKGFPG KDIENGITN 
      ATRCGPCPS KCDVCESNG ICSARRDVY LKTAILSIQ 
      LGCMAITVI IALVVLKQR KTKSIASGM WTILETILV 
      GIFILYCTV VVRFFEPSV VQCLLEPWA REVGFIICY 
      GAIILKLYR HLIEFRTRK AHRWVVKDT DLLKYLLIM 
      IMSVLAYMA AYTSITLNF LKENYSLLR EEVTQDGVH 
      YQACKPLWW DYVTETGEL VILIFGIHL SYASRNART 
      QFHERSFLC AAISIELAV STLFYVGRI LLLPGLHPD 
      FVLIIYCLR TQLSTSMTL MLVFIPKFW YQQKQVRSL 
      AQEYSCRIP VDAFKDVNA HGPLTGNNS DVDVGEVTL 
      ADMSPDDIR AELKRLYLQ LELFKSKTI CRDNPHISK 
      RRGGRKAQH RRFSLQKKG SREKNLHGK HRVQKQEET 
      TEAEPSRTP EDSVCSNEG PSAIYSDLP STAHTTDHK
    • >TC013504 RB
      MNEAVVLLL FLTSVHAAN DNEDCKIRF LSKPTAPSR 
      LNLNVTAVT SSIFANRAS DNFSDIARL TLTTHPEIL 
      GLVLKSPNE QILALNVAQ RLRIFSNKT VENEAFWTG 
      LGNQTQGWT PAFRVCHFL KGSWFYGFV ATKSNLTTA 
      LFLNLQLDQ CDDNLEEIF GKRHKCDQE TTYCVPQPE 
      ENGGYSCFC RQGFFHPDA SLSWKGFPG KDIENGITN 
      ATRCGPCPS KCDVCESNG ICSARRDVY LKTAILSIQ 
      LGCMAITVI IALVVLKQR KTKSIASGM WTILETILV 
      GIFILYCTV VVRFFEPSV VQCLLEPWA REVGFIICY 
      GAIILKLYR HLIEFRTRK AHRWVVKDT DLLKYLLIM 
      IMSVLAYMA AYTSITLNF LKENYSLLR EEVTQDGVH 
      YQACKPLWW DYVTETGEL VILIFGIHL SYASRNART 
      QFHERSFLC AAISIELAV STLFYVGRI LLLPGLHPD 
      FVLIIYCLR TQLSTSMTL MLVFIPKFW YQQKQVRSL 
      AQEYSCRIP VDAFKDVNA HGPLTGNNS DVDVGEVTL 
      ADMSPDDIR AELKRLYLQ LELFKSKTI CRDNPHISK 
      RRGGRKAQH RRFSLQNLH GKHRVQKQE ETTEAEPSR 
      TPEDSVCSN EGPSAIYSD LPSTAHTTD HK
    CDS sequence
    • >TC013504 RA
      atgaatgaa gctgttgtc ctcttactc ttcttgacg 
      tccgtacat gcagcaaac gacaatgaa gattgtaaa 
      atccgattt ctttctaaa ccaaccgct ccatccaga 
      ctgaacttg aacgttact gctgttaca agctctatt 
      tttgctaat cgtgctagt gataatttt tcggacatc 
      gcccgtctt actttgacc acacatcct gaaatactc 
      ggacttgta ctcaagtct cccaacgaa caaattctg 
      gctctcaac gttgcccaa agactgagg attttttct 
      aataagact gttgaaaat gaagctttc tggacaggt 
      ttaggaaat cagacgcag gggtggacg ccggctttc 
      agagtctgt catttcctg aaaggcagc tggttttac 
      ggttttgtc gccaccaag agcaacctc accaccgcc 
      ctgtttctg aatttacaa ctggatcaa tgcgacgac 
      aatttggaa gagattttc ggcaaacgc cacaaatgc 
      gaccaggaa accacatac tgcgtcccg caaccagag 
      gagaacggc gggtattcc tgcttctgt cggcaagga 
      ttcttccat cctgacgcc agcctcagc tggaagggc 
      tttccgggg aaggacatc gagaacgga atcaccaac 
      gcgacacgg tgcggccct tgtccttcg aaatgcgac 
      gtttgcgaa tcaaatggg atatgttcg gctcgcaga 
      gacgtttat ttgaaaact gcgattctc agcatccaa 
      ctcggatgc atggcaata actgtcatt attgccttg 
      gtggtctta aagcagcgc aaaacgaag tcgattgcc 
      tccggcatg tggacgatc ctggaaacg atcctagtc 
      ggcatattc atcttgtat tgtacggtg gtggtgcgc 
      tttttcgag ccgtcggtg gtgcagtgt ttgctggag 
      ccgtgggcc cgagaggtg ggcttcatc atctgctac 
      ggcgcgatt atcctcaag ctgtaccgg catctgatc 
      gaatttcgc acaaggaag gcccacaga tgggtcgtc 
      aaagacacg gacctcctc aagtacctc ctcataatg 
      ataatgtcg gtgctggcc tacatggcg gcgtacacc 
      tcaatcacc ctcaacttt ctcaaagaa aactacagt 
      ctgctgagg gaggaggtc acccaggac ggggtgcac 
      taccaagcc tgcaagccc ctgtggtgg gactacgtg 
      acggaaaca ggggaactg gttattctc atatttggg 
      atacatctg agctacgcg agcaggaac gcccggacg 
      caatttcac gagcggagt ttcctgtgt gccgcaatc 
      agcatcgag ttggcagtg agcaccctt ttttacgta 
      ggacgtatc ttgttactt cccggactt catccggac 
      ttcgtttta atcatatat tgcttacga acgcagttg 
      tcgacaagt atgaccttg atgttggta ttcatcccg 
      aaattttgg taccagcaa aagcaagtg cgctctctc 
      gcccaggag tattcctgc cggatacca gtagatgct 
      ttcaaggat gttaacgca cacggtcca ctaacgggc 
      aacaatagc gatgtggac gtgggtgag gtcactctg 
      gccgacatg agtccagac gacatcagg gccgaactc 
      aaaagactc tacctgcag ctggagctc ttcaagagc 
      aaaaccatc tgcagggac aatccacac atatcgaag 
      cgccgcggc ggacgcaag gcccaacac cgcaggttc 
      agcttgcag aaaaaagga agtagagag aagaatttg 
      catggaaag caccgcgtg cagaagcag gaagagacg 
      acagaggcg gaaccgtcc aggacgccg gaagattcg 
      gtttgcagc aacgagggc cccagcgcc atttacagc 
      gacctacca tcgaccgct cacaccacc gaccataaa 
      tag
    • >TC013504 RB
      atgaatgaa gctgttgtc ctcttactc ttcttgacg 
      tccgtacat gcagcaaac gacaatgaa gattgtaaa 
      atccgattt ctttctaaa ccaaccgct ccatccaga 
      ctgaacttg aacgttact gctgttaca agctctatt 
      tttgctaat cgtgctagt gataatttt tcggacatc 
      gcccgtctt actttgacc acacatcct gaaatactc 
      ggacttgta ctcaagtct cccaacgaa caaattctg 
      gctctcaac gttgcccaa agactgagg attttttct 
      aataagact gttgaaaat gaagctttc tggacaggt 
      ttaggaaat cagacgcag gggtggacg ccggctttc 
      agagtctgt catttcctg aaaggcagc tggttttac 
      ggttttgtc gccaccaag agcaacctc accaccgcc 
      ctgtttctg aatttacaa ctggatcaa tgcgacgac 
      aatttggaa gagattttc ggcaaacgc cacaaatgc 
      gaccaggaa accacatac tgcgtcccg caaccagag 
      gagaacggc gggtattcc tgcttctgt cggcaagga 
      ttcttccat cctgacgcc agcctcagc tggaagggc 
      tttccgggg aaggacatc gagaacgga atcaccaac 
      gcgacacgg tgcggccct tgtccttcg aaatgcgac 
      gtttgcgaa tcaaatggg atatgttcg gctcgcaga 
      gacgtttat ttgaaaact gcgattctc agcatccaa 
      ctcggatgc atggcaata actgtcatt attgccttg 
      gtggtctta aagcagcgc aaaacgaag tcgattgcc 
      tccggcatg tggacgatc ctggaaacg atcctagtc 
      ggcatattc atcttgtat tgtacggtg gtggtgcgc 
      tttttcgag ccgtcggtg gtgcagtgt ttgctggag 
      ccgtgggcc cgagaggtg ggcttcatc atctgctac 
      ggcgcgatt atcctcaag ctgtaccgg catctgatc 
      gaatttcgc acaaggaag gcccacaga tgggtcgtc 
      aaagacacg gacctcctc aagtacctc ctcataatg 
      ataatgtcg gtgctggcc tacatggcg gcgtacacc 
      tcaatcacc ctcaacttt ctcaaagaa aactacagt 
      ctgctgagg gaggaggtc acccaggac ggggtgcac 
      taccaagcc tgcaagccc ctgtggtgg gactacgtg 
      acggaaaca ggggaactg gttattctc atatttggg 
      atacatctg agctacgcg agcaggaac gcccggacg 
      caatttcac gagcggagt ttcctgtgt gccgcaatc 
      agcatcgag ttggcagtg agcaccctt ttttacgta 
      ggacgtatc ttgttactt cccggactt catccggac 
      ttcgtttta atcatatat tgcttacga acgcagttg 
      tcgacaagt atgaccttg atgttggta ttcatcccg 
      aaattttgg taccagcaa aagcaagtg cgctctctc 
      gcccaggag tattcctgc cggatacca gtagatgct 
      ttcaaggat gttaacgca cacggtcca ctaacgggc 
      aacaatagc gatgtggac gtgggtgag gtcactctg 
      gccgacatg agtccagac gacatcagg gccgaactc 
      aaaagactc tacctgcag ctggagctc ttcaagagc 
      aaaaccatc tgcagggac aatccacac atatcgaag 
      cgccgcggc ggacgcaag gcccaacac cgcaggttc 
      agcttgcag aatttgcat ggaaagcac cgcgtgcag 
      aagcaggaa gagacgaca gaggcggaa ccgtccagg 
      acgccggaa gattcggtt tgcagcaac gagggcccc 
      agcgccatt tacagcgac ctaccatcg accgctcac 
      accaccgac cataaatag
    mRNA sequence
    • >TC013504 RA
      tcagtttaa cttcgctcg ctactcaac tttaaaatg 
      ctaacgtaa tgtctattc ggcaccttt tctaagact 
      ttctatagt gtcatgtga gtggttcat ttcctcttt 
      cttgctgtc ttctctgtt aagcgttgc gtctccctt 
      gaaagcatg aagttgagg atgatataa tgacggtat 
      gaatgaagc tgttgtcct cttactctt cttgacgtc 
      cgtacatgc agcaaacga caatgaaga ttgtaaaat 
      ccgatttct ttctaaacc aaccgctcc atccagact 
      gaacttgaa cgttactgc tgttacaag ctctatttt 
      tgctaatcg tgctagtga taatttttc ggacatcgc 
      ccgtcttac tttgaccac acatcctga aatactcgg 
      acttgtact caagtctcc caacgaaca aattctggc 
      tctcaacgt tgcccaaag actgaggat tttttctaa 
      taagactgt tgaaaatga agctttctg gacaggttt 
      aggaaatca gacgcaggg gtggacgcc ggctttcag 
      agtctgtca tttcctgaa aggcagctg gttttacgg 
      ttttgtcgc caccaagag caacctcac caccgccct 
      gtttctgaa tttacaact ggatcaatg cgacgacaa 
      tttggaaga gattttcgg caaacgcca caaatgcga 
      ccaggaaac cacatactg cgtcccgca accagagga 
      gaacggcgg gtattcctg cttctgtcg gcaaggatt 
      cttccatcc tgacgccag cctcagctg gaagggctt 
      tccggggaa ggacatcga gaacggaat caccaacgc 
      gacacggtg cggcccttg tccttcgaa atgcgacgt 
      ttgcgaatc aaatgggat atgttcggc tcgcagaga 
      cgtttattt gaaaactgc gattctcag catccaact 
      cggatgcat ggcaataac tgtcattat tgccttggt 
      ggtcttaaa gcagcgcaa aacgaagtc gattgcctc 
      cggcatgtg gacgatcct ggaaacgat cctagtcgg 
      catattcat cttgtattg tacggtggt ggtgcgctt 
      tttcgagcc gtcggtggt gcagtgttt gctggagcc 
      gtgggcccg agaggtggg cttcatcat ctgctacgg 
      cgcgattat cctcaagct gtaccggca tctgatcga 
      atttcgcac aaggaaggc ccacagatg ggtcgtcaa 
      agacacgga cctcctcaa gtacctcct cataatgat 
      aatgtcggt gctggccta catggcggc gtacacctc 
      aatcaccct caactttct caaagaaaa ctacagtct 
      gctgaggga ggaggtcac ccaggacgg ggtgcacta 
      ccaagcctg caagcccct gtggtggga ctacgtgac 
      ggaaacagg ggaactggt tattctcat atttgggat 
      acatctgag ctacgcgag caggaacgc ccggacgca 
      atttcacga gcggagttt cctgtgtgc cgcaatcag 
      catcgagtt ggcagtgag caccctttt ttacgtagg 
      acgtatctt gttacttcc cggacttca tccggactt 
      cgttttaat catatattg cttacgaac gcagttgtc 
      gacaagtat gaccttgat gttggtatt catcccgaa 
      attttggta ccagcaaaa gcaagtgcg ctctctcgc 
      ccaggagta ttcctgccg gataccagt agatgcttt 
      caaggatgt taacgcaca cggtccact aacgggcaa 
      caatagcga tgtggacgt gggtgaggt cactctggc 
      cgacatgag tccagacga catcagggc cgaactcaa 
      aagactcta cctgcagct ggagctctt caagagcaa 
      aaccatctg cagggacaa tccacacat atcgaagcg 
      ccgcggcgg acgcaaggc ccaacaccg caggttcag 
      cttgcagaa aaaaggaag tagagagaa gaatttgca 
      tggaaagca ccgcgtgca gaagcagga agagacgac 
      agaggcgga accgtccag gacgccgga agattcggt 
      ttgcagcaa cgagggccc cagcgccat ttacagcga 
      cctaccatc gaccgctca caccaccga ccataaata 
      gagcagata gcccatttc gcaatgtga atttgtatc 
      actgtagtc gtgtacgca tccgttatt tagagattt 
      ttgtagatc tgtgttgga ctaaaaacc tcactaaac 
      acttttata aactgcaac ctaaaatct gccctaatc 
      tagcgcgta agcaacgat tcctttcta aacaaaagg 
      tgataaagt gaaatctct aaataaccg aaatggata 
      aatcaaaag tacgatcaa acctaaaca atcctcatt 
      caaattgcg tccaaattc attttgtac ccctagtta 
      acgtgcttc gtgcaattt tgtatcatg ttcagctta 
      gtctagaag cttaagcat cgactcttt gttgcattt 
      tagattgag agggtatgg gaccaacta gcccaaaat 
      ccgttttgt ttgtaacgc agaattcaa tttttcgaa 
      ttgggagtc ttgtcaaga cattagtga tattatcga 
      agggtcttt tcattattg tttgtcatt gtatgtatt 
      tttaccaac taattacat cattatgta aaaagcgag 
      ttagagccg agttgtata ataaactat tgttttgta 
      t
    • >TC013504 RB
      tcagtttaa cttcgctcg ctactcaac tttaaaatg 
      ctaacgtaa tgtctattc ggcaccttt tctaagact 
      ttctatagt gtcatgtga gtggttcat ttcctcttt 
      cttgctgtc ttctctgtt aagcgttgc gtctccctt 
      gaaagcatg aagttgagg atgatataa tgacggtat 
      gaatgaagc tgttgtcct cttactctt cttgacgtc 
      cgtacatgc agcaaacga caatgaaga ttgtaaaat 
      ccgatttct ttctaaacc aaccgctcc atccagact 
      gaacttgaa cgttactgc tgttacaag ctctatttt 
      tgctaatcg tgctagtga taatttttc ggacatcgc 
      ccgtcttac tttgaccac acatcctga aatactcgg 
      acttgtact caagtctcc caacgaaca aattctggc 
      tctcaacgt tgcccaaag actgaggat tttttctaa 
      taagactgt tgaaaatga agctttctg gacaggttt 
      aggaaatca gacgcaggg gtggacgcc ggctttcag 
      agtctgtca tttcctgaa aggcagctg gttttacgg 
      ttttgtcgc caccaagag caacctcac caccgccct 
      gtttctgaa tttacaact ggatcaatg cgacgacaa 
      tttggaaga gattttcgg caaacgcca caaatgcga 
      ccaggaaac cacatactg cgtcccgca accagagga 
      gaacggcgg gtattcctg cttctgtcg gcaaggatt 
      cttccatcc tgacgccag cctcagctg gaagggctt 
      tccggggaa ggacatcga gaacggaat caccaacgc 
      gacacggtg cggcccttg tccttcgaa atgcgacgt 
      ttgcgaatc aaatgggat atgttcggc tcgcagaga 
      cgtttattt gaaaactgc gattctcag catccaact 
      cggatgcat ggcaataac tgtcattat tgccttggt 
      ggtcttaaa gcagcgcaa aacgaagtc gattgcctc 
      cggcatgtg gacgatcct ggaaacgat cctagtcgg 
      catattcat cttgtattg tacggtggt ggtgcgctt 
      tttcgagcc gtcggtggt gcagtgttt gctggagcc 
      gtgggcccg agaggtggg cttcatcat ctgctacgg 
      cgcgattat cctcaagct gtaccggca tctgatcga 
      atttcgcac aaggaaggc ccacagatg ggtcgtcaa 
      agacacgga cctcctcaa gtacctcct cataatgat 
      aatgtcggt gctggccta catggcggc gtacacctc 
      aatcaccct caactttct caaagaaaa ctacagtct 
      gctgaggga ggaggtcac ccaggacgg ggtgcacta 
      ccaagcctg caagcccct gtggtggga ctacgtgac 
      ggaaacagg ggaactggt tattctcat atttgggat 
      acatctgag ctacgcgag caggaacgc ccggacgca 
      atttcacga gcggagttt cctgtgtgc cgcaatcag 
      catcgagtt ggcagtgag caccctttt ttacgtagg 
      acgtatctt gttacttcc cggacttca tccggactt 
      cgttttaat catatattg cttacgaac gcagttgtc 
      gacaagtat gaccttgat gttggtatt catcccgaa 
      attttggta ccagcaaaa gcaagtgcg ctctctcgc 
      ccaggagta ttcctgccg gataccagt agatgcttt 
      caaggatgt taacgcaca cggtccact aacgggcaa 
      caatagcga tgtggacgt gggtgaggt cactctggc 
      cgacatgag tccagacga catcagggc cgaactcaa 
      aagactcta cctgcagct ggagctctt caagagcaa 
      aaccatctg cagggacaa tccacacat atcgaagcg 
      ccgcggcgg acgcaaggc ccaacaccg caggttcag 
      cttgcagaa tttgcatgg aaagcaccg cgtgcagaa 
      gcaggaaga gacgacaga ggcggaacc gtccaggac 
      gccggaaga ttcggtttg cagcaacga gggccccag 
      cgccattta cagcgacct accatcgac cgctcacac 
      caccgacca taaatagag cagatagcc catttcgca 
      atgtgaatt tgtatcact gtagtcgtg tacgcatcc 
      gttatttag agatttttg tagatctgt gttggacta 
      aaaacctca ctaaacact tttataaac tgcaaccta 
      aaatctgcc ctaatctag cgcgtaagc aacgattcc 
      tttctaaac aaaaggtga taaagtgaa atctctaaa 
      taaccgaaa tggataaat caaaagtac gatcaaacc 
      taaacaatc ctcattcaa attgcgtcc aaattcatt 
      ttgtacccc tagttaacg tgcttcgtg caattttgt 
      atcatgttc agcttagtc tagaagctt aagcatcga 
      ctctttgtt gcattttag attgagagg gtatgggac 
      caactagcc caaaatccg ttttgtttg taacgcaga 
      attcaattt ttcgaattg ggagtcttg tcaagacat 
      tagtgatat tatcgaagg gtcttttca ttattgttt 
      gtcattgta tgtattttt accaactaa ttacatcat 
      tatgtaaaa agcgagtta gagccgagt tgtataata 
      aactattgt tttgtat
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