Status
Please wait ...
Gene information
General information
TC033129
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013472
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9088176..9090399
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033129
    iBeetle screen iB_05438

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 6
    tropharium structure irregular - Number of animals showing the phenotype: 6

    egg chamber not present - Number of animals showing the phenotype: 6

    Comment: Tropharia-lateral oviducts connection weakened.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033129 RA
      MPGKQGREG LHGIPGEKG DRGLPGLPG RSGTPGLPG 
      PPGSKGDKG DRGFPGPVG PPGNMGPPG RDGYAGAPG 
      LQGEKGDRG PPGLIGAPG EKGDRGITG LQGPPGYPG 
      EKGDRGYPG ISGPPGPVG PTGEKGSPG APGALGIPG 
      FHGPKGDKG EPAPPGFPG PKGDKGVPG QPGLPGERG 
      LPGPVGPPG RDGFPGEKG DRGVDGLPG SPGPPGPKG 
      DRGFDGAIG IAGPVGEPG PKGEPGAPC EQVGDYLTG 
      NLLVRHSQS IEIPQCESG HVKLWEGYS LLYVQGNEK 
      AHSQDLGFA GSCVRKFNT MPFVFCDFN NVCNYASRN 
      DKSYWLSTS APIPMMPVE ESAIRQYIS RCVVCEAPS 
      NVIAVHSQS LSLPDCPHG WSSLWIGYS FIMHTSDGA 
      EGGGQSLVS PGSCLEDFR ASPFIECNG AQGTCHYFA 
      NTLSFWLAT IEPYQEFQK PQKQTLKAG NVRDRISRC 
      QVCIKNT
    CDS sequence
    • >TC033129 RA
      atgcccggc aagcaaggc cgcgagggt ctgcacgga 
      atccctgga gaaaaagga gatcgaggt cttccgggt 
      cttccaggc cgttccgga acgcctgga cttcccgga 
      cccccaggt tctaaagga gataaaggc gatcgaggt 
      tttccaggt cctgtcggg cctccagga aatatgggt 
      ccacccgga cgtgacggc tacgcggga gctcctggt 
      cttcaagga gaaaaaggt gacagagga ccacccggc 
      cttattgga gcacctggt gaaaaaggc gatcgagga 
      ataactgga cttcaagga ccccctggg tatccaggc 
      gaaaagggt gaccgtgga tatcccgga atttctgga 
      cccccggga cctgttgga ccaactgga gaaaaaggt 
      tcacctggc gctcctggc gcccttgga attccagga 
      ttccatgga cctaaaggc gataaggga gaaccagcg 
      ccgcccggt ttccccgga cctaaggga gataaagga 
      gtaccagga cagccagga ttacctgga gaaagggga 
      ttgccgggt ccggtggga ccccctggt cgagatggg 
      ttccccggt gaaaaggga gacagagga gttgatgga 
      ttaccggga tccccgggt ccaccgggt cctaaaggg 
      gacagagga ttcgatggt gcgattggt attgctggc 
      cctgtgggt gaaccaggt cccaaagga gaaccgggg 
      gctccatgt gaacaagtc ggagattac ctcacgggg 
      aatcttcta gtaagacac agccaaagt atcgagatt 
      ccgcagtgc gaaagcggc cacgtgaaa ctatgggaa 
      ggctattct ctactctat gttcaagga aacgaaaaa 
      gcccattca caagactta ggctttgcc ggctcttgt 
      gtgagaaaa ttcaacact atgcctttt gttttctgc 
      gacttcaac aatgtttgt aattacgcc agcagaaat 
      gataagtct tattggttg tcaacaagc gccccgatt 
      ccaatgatg ccggtcgaa gagtcagct ataagacaa 
      tacatttcc aggtgtgtt gtctgtgaa gcgccatct 
      aatgtcatc gcggtgcac agccaatca ctatccttg 
      cctgactgt ccgcacggc tggagctct ctctggatt 
      ggctacagt tttattatg cacacgtca gatggcgct 
      gaaggcggt ggtcagtcc ctggtgagt ccgggttct 
      tgccttgaa gatttccgg gcttcgccg ttcatcgaa 
      tgcaacggt gcccaaggc acgtgccac tacttcgcc 
      aatactctt agcttctgg ctggcgacc atcgagccc 
      taccaggag ttccagaag ccgcagaag cagacgctg 
      aaagccggc aacgtccgc gaccggata agtcgatgc 
      caagtgtgc atcaagaac acgtaa
    mRNA sequence
    • >TC033129 RA
      gtttcccgg aattgccgg acttccggg tgctactgt 
      aaaaggcga aaaaggcct tcccggtat gcccggcaa 
      gcaaggccg cgagggtct gcacggaat ccctggaga 
      aaaaggaga tcgaggtct tccgggtct tccaggccg 
      ttccggaac gcctggact tcccggacc cccaggttc 
      taaaggaga taaaggcga tcgaggttt tccaggtcc 
      tgtcgggcc tccaggaaa tatgggtcc acccggacg 
      tgacggcta cgcgggagc tcctggtct tcaaggaga 
      aaaaggtga cagaggacc acccggcct tattggagc 
      acctggtga aaaaggcga tcgaggaat aactggact 
      tcaaggacc ccctgggta tccaggcga aaagggtga 
      ccgtggata tcccggaat ttctggacc cccgggacc 
      tgttggacc aactggaga aaaaggttc acctggcgc 
      tcctggcgc ccttggaat tccaggatt ccatggacc 
      taaaggcga taagggaga accagcgcc gcccggttt 
      ccccggacc taagggaga taaaggagt accaggaca 
      gccaggatt acctggaga aaggggatt gccgggtcc 
      ggtgggacc ccctggtcg agatgggtt ccccggtga 
      aaagggaga cagaggagt tgatggatt accgggatc 
      cccgggtcc accgggtcc taaagggga cagaggatt 
      cgatggtgc gattggtat tgctggccc tgtgggtga 
      accaggtcc caaaggaga accgggggc tccatgtga 
      acaagtcgg agattacct cacggggaa tcttctagt 
      aagacacag ccaaagtat cgagattcc gcagtgcga 
      aagcggcca cgtgaaact atgggaagg ctattctct 
      actctatgt tcaaggaaa cgaaaaagc ccattcaca 
      agacttagg ctttgccgg ctcttgtgt gagaaaatt 
      caacactat gccttttgt tttctgcga cttcaacaa 
      tgtttgtaa ttacgccag cagaaatga taagtctta 
      ttggttgtc aacaagcgc cccgattcc aatgatgcc 
      ggtcgaaga gtcagctat aagacaata catttccag 
      gtgtgttgt ctgtgaagc gccatctaa tgtcatcgc 
      ggtgcacag ccaatcact atccttgcc tgactgtcc 
      gcacggctg gagctctct ctggattgg ctacagttt 
      tattatgca cacgtcaga tggcgctga aggcggtgg 
      tcagtccct ggtgagtcc gggttcttg ccttgaaga 
      tttccgggc ttcgccgtt catcgaatg caacggtgc 
      ccaaggcac gtgccacta cttcgccaa tactcttag 
      cttctggct ggcgaccat cgagcccta ccaggagtt 
      ccagaagcc gcagaagca gacgctgaa agccggcaa 
      cgtccgcga ccggataag tcgatgcca agtgtgcat 
      caagaacac gtaatatcg ccggtaaac cttaggctt 
      attttcgac gagtcgatg ggtcagtcg catcgcggt 
      gcgaatgac ccatccgat catgtgttc aacctctag 
      tgttcctcg aatctcgag ttcttgtac atcgggcgt 
      acggatgat ggacgagaa ctcgcattt ttgtatcat 
      aagctttgt aacgtttta atactgccg tgccatgtt 
      tgtgtaaca aaaaagcat gtaaagagt tttgtatag 
      tacttagtt taagttgta tgtatgtaa taataccac 
      aaggctaca tgaaatggt atttgcgaa aaattcatt 
      aattagggc ggtcacagt tcttagaaa tttcgctgt 
      gatttttac tagcatttt ttaagcaga atggttatc 
      ttttaaaga gaatatttt tgtaattaa taattttaa 
      tggtgctat gtatccctg taataattt atgtgttcg 
      cttcaagtc cgcagtgat cttgttcta ccatttata 
      cccaacaca cacttttac actttttaa tctcatcat 
      taacattcc tttttgttc ttcgttcta aacttctag 
      tcaacaacg tggctacaa aacttcaaa ttcgtcaga 
      tatcactgc atgtttgta atctttcca gctctttaa 
      acttttttt tattaaaac actgccatt gtaaaataa 
      atattactt aaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY