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Gene information
General information
TC013459 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9250402..9253360
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013459
    iBeetle screen iB_05437

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013459 RA
      MTVKKFYKS FPKNYLQGP SIQNVPIFR NFAHHKTPA 
      SSTKSFSKI LVANRGEIA CRIIKTAKK MNLKTVAVY 
      SEADAFSPH VKLADEAVF LGPSLASES YLNIKKIID 
      AVKSTGAEA VHPGYGFLS ENASFVSLL EDEKIVFVG 
      PPAGVINKL GDKLASKKL AQNAGVNVI PGFDGEIRD 
      AKHCVEIAR QIGYPVMIK ASAGGGGKG MRIARNDDD 
      ARNGFQMST EEAASSFGD NRILLEKLI ESPRHVEVQ 
      VLGDKHGRI VYLNERECS IQRRNQKIV EEAPSTFLD 
      VETRKAMGE QAVRLCTDI GYHSAGTVE FLVDKNRAF 
      YFLEMNTRL QVEHPVTEA ITGVDIVQQ MIRIAQGYP 
      LDVTQEDVK INGHAIECR IYAEDPYKN LGSPSIGKL 
      FKYREPNRV QGIRCDSGV EEGSEITFY YDSMICKLI 
      CFGKDRQEA IKKSTQALD YYVVRGVTH NVPLLRSIL 
      SHERFAIGD INTNFLSEA YPNGFGGHE LNLREKNQL 
      LAIASAFYV RNDSRNRVN NPTQQNEWN VVVKLNRDT 
      HTVRISERC DFFEIEIND SKVYKINKM GLNLAEPVT 
      EIKLNGEQA VVQLISKNP SGDYKISYL GTTYDLNII 
      PKKAADLLH LMPQELQPD ISKTIKSPM PGLIKSLSC 
      KEGDCITEG QELCTIEAM KMQNSIIAL TSGKIKTVY 
      IKEGSVVSD NDVLIDLE
    CDS sequence
    • >TC013459 RA
      atgacagta aagaaattt tataaaagt tttcctaaa 
      aattacctt caagggccc agtatacag aatgttcca 
      atatttagg aattttgcg caccataag acaccagcg 
      tcttcaacc aaaagtttt agtaaaatt ttagtggcc 
      aatagaggg gaaattgcg tgcagaatc ataaaaacc 
      gccaagaag atgaattta aaaacggtg gctgtttac 
      tctgaagct gatgcattc tctccccat gtgaagtta 
      gccgacgag gcagtattt ttggggccg tctttggcg 
      tccgaatcc tacttaaac attaaaaaa atcatcgat 
      gcggttaaa tccaccggt gcagaggcg gtgcacccg 
      ggttacggt ttcttgtcc gagaacgcg tctttcgtt 
      tctctgctg gaggatgaa aaaatagtt ttcgttgga 
      cccccagct ggggtaatt aataaactc ggtgacaaa 
      ttagcatca aagaagctc gctcaaaat gcaggcgtt 
      aacgtaatt ccaggattc gacggtgaa ataagggat 
      gctaaacac tgtgttgaa atagctcgg caaattgga 
      tatccggta atgataaag gcatctgct ggtggcggt 
      ggcaaagga atgagaatt gcaagaaac gacgacgat 
      gcaaggaac ggctttcaa atgtcaact gaagaagcc 
      gcttctagc ttcggagat aatcgtatt ttacttgag 
      aaacttata gaaagccct aggcacgtt gaagtacaa 
      gtcttagga gataaacac gggaggatt gtttaccta 
      aacgagaga gagtgcagc attcagcgc agaaatcaa 
      aaaattgta gaagaggcg ccgagtacg tttctggac 
      gtggaaact cgaaaagct atgggcgag caggccgtg 
      cggctttgc accgacatc ggctaccac tccgcagga 
      actgtcgag tttttagtg gacaagaat cgcgcgttt 
      tatttcctg gaaatgaac acaagacta caagtggaa 
      catcccgtc accgaggcg ataacaggg gttgacata 
      gtgcaacaa atgattcga atcgcccaa ggatatcca 
      ctcgatgta actcaagaa gacgtaaaa attaacgga 
      cacgccatt gaatgtcga atttacgcc gaggatcct 
      tacaaaaac ctgggttcc ccgagtatt ggaaaactt 
      tttaaatat agagagccg aatagggtt cagggaata 
      agatgtgat agtggtgtt gaagaaggc agcgaaatc 
      actttctat tacgactca atgatctgc aaactaata 
      tgttttggg aaagatcga caagaagcc atcaaaaaa 
      agcacacaa gcacttgat tattacgtt gtgagagga 
      gttacgcac aatgtgcca cttttgcgg agcatcctg 
      tcgcatgaa cgtttcgca atcggggac ataaacaca 
      aatttctta tcggaagcg tatccgaac ggctttggc 
      ggacatgag cttaatttg cgcgaaaaa aatcaacta 
      cttgctata gcctcagcg ttttacgtg aggaatgac 
      tcgagaaat cgggtgaat aatcccacc caacaaaac 
      gagtggaat gttgttgta aaactgaat cgcgacact 
      cacactgta cgcatttcc gagcgatgt gattttttc 
      gaaatagag atcaacgat tccaaagtg tacaaaata 
      aataaaatg ggtttaaat ttagccgaa ccggtgaca 
      gaaattaaa ttaaatgga gagcaagct gttgtgcaa 
      ttgatttct aaaaaccct agcggtgat tacaaaatc 
      tcctacctt ggaactacc tacgattta aatataatt 
      cccaagaaa gcggcggat ttgttacac ctaatgccc 
      caagaactt cagccagat atatctaaa acaattaaa 
      tccccgatg ccaggttta attaaaagt ttatcttgc 
      aaagaaggc gattgtata accgagggc caagagttg 
      tgtacaatt gaggccatg aagatgcaa aattccata 
      attgcactc acttcgggc aaaattaaa acggtttac 
      atcaaggaa ggcagcgta gttagcgat aacgacgtt 
      ttaatcgac ttggaataa
    mRNA sequence
    • >TC013459 RA
      cgagataaa aagatcaag tgcatgtga ctactagta 
      gtattacaa aatgacagt aaagaaatt ttataaaag 
      ttttcctaa aaattacct tcaagggcc cagtataca 
      gaatgttcc aatatttag gaattttgc gcaccataa 
      gacaccagc gtcttcaac caaaagttt tagtaaaat 
      tttagtggc caatagagg ggaaattgc gtgcagaat 
      cataaaaac cgccaagaa gatgaattt aaaaacggt 
      ggctgttta ctctgaagc tgatgcatt ctctcccca 
      tgtgaagtt agccgacga ggcagtatt tttggggcc 
      gtctttggc gtccgaatc ctacttaaa cattaaaaa 
      aatcatcga tgcggttaa atccaccgg tgcagaggc 
      ggtgcaccc gggttacgg tttcttgtc cgagaacgc 
      gtctttcgt ttctctgct ggaggatga aaaaatagt 
      tttcgttgg acccccagc tggggtaat taataaact 
      cggtgacaa attagcatc aaagaagct cgctcaaaa 
      tgcaggcgt taacgtaat tccaggatt cgacggtga 
      aataaggga tgctaaaca ctgtgttga aatagctcg 
      gcaaattgg atatccggt aatgataaa ggcatctgc 
      tggtggcgg tggcaaagg aatgagaat tgcaagaaa 
      cgacgacga tgcaaggaa cggctttca aatgtcaac 
      tgaagaagc cgcttctag cttcggaga taatcgtat 
      tttacttga gaaacttat agaaagccc taggcacgt 
      tgaagtaca agtcttagg agataaaca cgggaggat 
      tgtttacct aaacgagag agagtgcag cattcagcg 
      cagaaatca aaaaattgt agaagaggc gccgagtac 
      gtttctgga cgtggaaac tcgaaaagc tatgggcga 
      gcaggccgt gcggctttg caccgacat cggctacca 
      ctccgcagg aactgtcga gtttttagt ggacaagaa 
      tcgcgcgtt ttatttcct ggaaatgaa cacaagact 
      acaagtgga acatcccgt caccgaggc gataacagg 
      ggttgacat agtgcaaca aatgattcg aatcgccca 
      aggatatcc actcgatgt aactcaaga agacgtaaa 
      aattaacgg acacgccat tgaatgtcg aatttacgc 
      cgaggatcc ttacaaaaa cctgggttc cccgagtat 
      tggaaaact ttttaaata tagagagcc gaatagggt 
      tcagggaat aagatgtga tagtggtgt tgaagaagg 
      cagcgaaat cactttcta ttacgactc aatgatctg 
      caaactaat atgttttgg gaaagatcg acaagaagc 
      catcaaaaa aagcacaca agcacttga ttattacgt 
      tgtgagagg agttacgca caatgtgcc acttttgcg 
      gagcatcct gtcgcatga acgtttcgc aatcgggga 
      cataaacac aaatttctt atcggaagc gtatccgaa 
      cggctttgg cggacatga gcttaattt gcgcgaaaa 
      aaatcaact acttgctat agcctcagc gttttacgt 
      gaggaatga ctcgagaaa tcgggtgaa taatcccac 
      ccaacaaaa cgagtggaa tgttgttgt aaaactgaa 
      tcgcgacac tcacactgt acgcatttc cgagcgatg 
      tgatttttt cgaaataga gatcaacga ttccaaagt 
      gtacaaaat aaataaaat gggtttaaa tttagccga 
      accggtgac agaaattaa attaaatgg agagcaagc 
      tgttgtgca attgatttc taaaaaccc tagcggtga 
      ttacaaaat ctcctacct tggaactac ctacgattt 
      aaatataat tcccaagaa agcggcgga tttgttaca 
      cctaatgcc ccaagaact tcagccaga tatatctaa 
      aacaattaa atccccgat gccaggttt aattaaaag 
      tttatcttg caaagaagg cgattgtat aaccgaggg 
      ccaagagtt gtgtacaat tgaggccat gaagatgca 
      aaattccat aattgcact cacttcggg caaaattaa 
      aacggttta catcaagga aggcagcgt agttagcga 
      taacgacgt tttaatcga cttggaata aactttttc 
      aacacgaaa aacgttttt tattcacca acgcacttc 
      aattattta gaaatatca atctgggaa acttgggcg 
      tttgcccaa atggtaaat tttgattaa tggtaaatt 
      aaaccgaaa ctcaaatag ggcgactgc aaccatggc 
      gttgtccaa acagtgaga ggatggttt tgcggtggc 
      ggccgtata aataagacg ggggcgcag atgtggccc 
      gccgggagc cccacgaaa gtgttaagc aggattaga 
      gcagccgcg tggtcgcat taagtacag acgaactaa 
      agtccttgt cctgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY