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Gene information
General information
TC013456 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9273122..9279198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013456
    iBeetle screen iB_05436

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 6

    wings orientation irregular - Number of animals showing the phenotype: 6
    wings partiallynecrotic - Number of animals showing the phenotype: 3
    Adult morphology
    elytra shortened - Number of animals showing the phenotype: 1

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013456 RA
      MNTRGDEPR PRGTFCGKS STLQEKVAQ FYYAHGLFC 
      STYPGALVF VAICATLLS CYPLLNLPL PGNSPLQTW 
      TSSVNSSSN VPPFCYIQQ VVLKSTVLP WGPELHLGD 
      AFRAPLYEA FKLLEAVRN YQDESTKKT LGHVCLHVE 
      SIRKSKSNY KNILPEYSC LVLSPANLW QQDVLKFTQ 
      DSSLLTTIF NHHNFQKGK TSITEMMFG MKVFDTGIK 
      RYPLRNRYR IIQYAITLF FKEYDEKFV HGLRRKLQS 
      VYSLHQNLE NGTNANFAT LNDTLIIQY PGEINLVEF 
      IPITLAFLI LFLYFYFSF RKIELIHSK LGMAFTSVL 
      TVCASLTMT YGLCFFFGL TLNLEGKVV FPYLVLLVG 
      LENVLCLTK SIVSSPLHL DVKIRIAQG LSKEGWSIT 
      KNLLLEITI LTFGIFTFV AVIQEFCIF AVVGLVTDY 
      FMQMIFFST VLGIDIRRK ENMVERHNL SFRSSLFQS 
      QVFYDKSFG IRRSKSHPR LSSFPANIV AGQVQGAQE 
      KKIPKRLKL VNIWTRTRF FHRAFMILM VIWISNIAY 
      NSGFIEHFI LNNSYFHEV AEQNSTSLE QNYSSIKLF 
      PLLNTNNSF INKVSYVTH SPLNYNQNQ TFDLENLKH 
      SDYEPWLKL SPQHWSSIV RKYNLSLSG QYIAILPNI 
      KLSHVIRPE QAVLLRNPN EKYGEKFQW QALVAALDP 
      IDFNGMETG TRSAIPQSE QPFYPTSVF AMILIAILC 
      FISVVVLGY TFVVLYRCI CSRNYAEWR ASWFSEKGE 
      ENVEEPVLL EAVPVVLEG HQQEIECTA TDGVNLVSA 
      SLDGQLKVW DSGTGELIA NVDRKAYFQ GCFESNCGT 
      SECDDNSSD YESGSPPSR GESYLFPRL KHRINTNFS 
      NVKEQCNSY NAKADFLKT YRELYFVRD LPKAKSRKE 
      MNRAKENKS ISFSESNQK FCDNGHTKF DSSDAKLSP 
      IWCMDYLEN LIVLGCADG RLEFWEGSS GRLKCIFED 
      GTDVGISNL KIVGSRIIA SRLYGTLEM FQLQTYNRG 
      HPVDWNFTC AYRRTHVRT GSVGSVPDH TNLISQNED 
      EEDFRCLKV FSVKAHQQP IICLDCEGG RILTGSQDH 
      TLKVFRLED GSPQYTLHG HCGPITCLF IDRISPATS 
      GSGSQDGML CVWDLLTGA CMYNIQAHD GTITSLTYS 
      ASYVISLGS DDRICVWER FQGHLLNTI YVSQTFSSQ 
      VLMLAPHLV VTARSGGLV IWDVRSGEC VRTIILGRS 
      PYIFIKQLM LLRDAVVCD YGNQLRIVR FPLITHKFD
    CDS sequence
    • >TC013456 RA
      atgaacacc cgtggggac gaaccccga cccaggggt 
      actttctgt ggaaaaagc tcaactctt caagagaaa 
      gttgcccaa ttttattac gcacatggt ctgttttgt 
      agcacatat cccggggct ttggtcttc gtcgcaatt 
      tgtgcaact ctgctctct tgttatcca ttattaaat 
      ttgcctctc cctggaaac tcgcctttg caaacatgg 
      acatcgagc gtgaattcc agctcgaat gtgccacct 
      ttttgctac attcagcaa gttgtgctg aagagcacg 
      gtcctgccg tggggcccc gagttacac ctaggtgac 
      gccttcagg gcgcctctt tacgaggct tttaaactt 
      ttggaagct gttagaaat taccaggat gagtccacg 
      aagaaaacg ctgggacat gtgtgctta catgtcgag 
      tcgattagg aaaagtaaa tcaaactac aaaaacatc 
      ctgccggag tatagttgt ttagtcctg tcgccggct 
      aacttgtgg caacaggac gtcctcaaa tttacgcag 
      gatagctct ctacttacc acaattttc aaccatcat 
      aacttccaa aaaggcaaa acatcaatc acggaaatg 
      atgttcggt atgaaggtg tttgacacc gggattaaa 
      cgatatccg ttgcgaaac cgctacaga atcattcag 
      tacgccata acacttttt ttcaaggaa tatgacgaa 
      aagtttgtt catggtttg cgcagaaag ctgcagtcg 
      gtgtattcc ttacaccaa aatttggaa aacggaaca 
      aatgctaac tttgccacg ctcaacgac actctcata 
      atccaatat cccggtgaa atcaacttg gtcgagttc 
      attcccatt actttggcg tttttaatc ctgttcctg 
      tacttttat ttctcgttt cgcaaaatc gaattaatt 
      cactcgaag ctcggaatg gcctttact tccgttctg 
      accgtgtgt gcaagcctc acaatgacc tacggcttg 
      tgttttttc ttcggccta actctgaac cttgaagga 
      aaagttgta tttccatat ttagtgttg ctagttggc 
      ttggaaaat gtcctatgt ttaacaaaa agcatagtt 
      tcgtcgccc ctccacctc gacgtgaaa atccgcata 
      gcgcagggt ttgagcaaa gaaggctgg tccatcacc 
      aaaaacctc ctcctggaa atcacgatt ttgacgttc 
      gggatcttc accttcgtc gcggtcatc caggagttt 
      tgcattttt gcggtcgtt ggtctcgtg accgactat 
      ttcatgcag atgattttc ttctcgacg gttttgggg 
      attgacatc cggcggaag gagaacatg gtggagaga 
      cacaatctg agctttcgg agcagtctc ttccaaagc 
      caggttttc tacgataag tcgttcggt attcggcga 
      tcgaagtcg cacccgcga ctgtcctcc ttccctgcc 
      aatatcgtg gcagggcag gtgcagggc gcccaagag 
      aaaaaaatc ccgaaacgg ttgaaactt gtgaatatt 
      tggaccagg acgcggttt ttccaccgc gcttttatg 
      atcttgatg gtgatttgg atttcgaat atcgcctac 
      aatagcggc ttcatcgag cattttatc ctgaataac 
      agttatttt cacgaggtg gccgaacaa aatagcact 
      agcttggag cagaactac tcgagtatc aagctgttt 
      ccgttgctg aacacgaat aattcgttc ataaacaaa 
      gtgagttat gttactcac agcccgctc aattacaat 
      cagaaccag acgttcgat ttggagaat ttgaagcat 
      tcggattac gagccgtgg ttgaaattg tcgcctcaa 
      cattggtcg tctattgtg cgaaaatac aacttgtcg 
      ttgagtgga caatacatt gccattttg ccaaatatt 
      aagctatcg cacgtgata cgtccagag caggcggtg 
      cttttgcgc aaccctaac gaaaaatac ggggagaag 
      ttccagtgg caggctttg gtggccgcc ctggacccc 
      attgacttc aatgggatg gagacaggc accaggtct 
      gccatacct cagtccgag cagcccttc tacccgacc 
      tcagttttc gcgatgatt ttgatcgcg attctgtgc 
      tttatcagc gtggtggtg ctggggtac acctttgtg 
      gtgctttac cggtgcata tgctcccgg aactacgcc 
      gaatggcgg gcgtcgtgg ttcagcgaa aagggggaa 
      gaaaacgtg gaagagcca gttttgttg gaggcggtg 
      ccggtggtg ctggaaggg caccaacag gagatcgaa 
      tgcacggcc acagacggg gttaatttg gtcagtgcc 
      tcgctagac gggcaactg aaggtttgg gatagtggg 
      accggggag ctgatcgcc aatgtggac aggaaggcg 
      tactttcag gggtgtttc gagtcgaat tgtggcact 
      tccgagtgc gatgataac tcgtcggat tatgagtcc 
      ggctctccg ccgtcccga ggggagagc tatctgttt 
      ccccgtctc aaacatcgg attaatacg aacttttca 
      aacgtgaaa gaacagtgt aactcgtat aatgccaaa 
      gctgatttt ttaaaaact tatcgagag ttgtatttt 
      gttcgcgat ttaccgaaa gctaaaagt cggaaagag 
      atgaacagg gccaaagaa aataaatcg attagtttt 
      agtgaaagt aatcaaaag ttttgtgat aatggacac 
      acgaaattt gatagtagt gatgctaaa ctttcaccg 
      atttggtgt atggactat ctggagaac ttgattgtt 
      ctggggtgt gctgatggg aggctggag ttttgggag 
      ggcagttcg gggaggttg aagtgcata tttgaagac 
      ggaaccgat gttggaata agcaattta aaaatcgtg 
      gggtcgcga attatcgca tcccgattg tatggcacc 
      ttggaaatg ttccaatta caaacttac aaccgtggc 
      catccagtt gactggaat ttcacttgc gcataccga 
      agaacccac gtaaggact ggctcagtt ggttccgtc 
      cctgatcac accaattta atttcacaa aacgaggat 
      gaggaagat ttccggtgt ttgaaagtt ttcagcgtt 
      aaggcacac caacagccg attatctgc ctcgactgt 
      gaaggcggc agaatttta accggcagc caagaccac 
      acgcttaaa gtgttccga ctggaggac ggcagcccc 
      cagtacacg ctgcacggc cactgcggc cccatcacc 
      tgtcttttc attgacagg atcagtcca gcgacttct 
      ggcagtggc tcccaggac ggaatgctc tgcgtttgg 
      gatcttcta actggtgcc tgtatgtac aacatccag 
      gcccatgat ggtacgata acatcgctg acgtactct 
      gcgagttac gtgatttca ctgggttct gacgaccgc 
      atttgtgtt tgggagcgc ttccaaggc catcttctg 
      aacaccatt tacgtttct cagaccttt tctagccag 
      gtgttgatg ttggcccct catttggtt gtcacagct 
      cgcagtgga gggctagtt atttgggat gtgaggtcg 
      ggtgaatgt gtccgaacg attatatta ggccgttca 
      ccgtatatt tttatcaaa cagttgatg ttgttacgc 
      gatgctgtg gtctgtgat tacggaaat caattgcgc 
      atcgtgagg ttccctctt atcactcat aaatttgac 
      tag
    mRNA sequence
    • >TC013456 RA
      tgcggcgtg tattttcgt aattcgtca tcctcaagt 
      gtatgaatg aggtgttta tcaattttt tgtaataaa 
      aacaatagt ttaaatcat tgtgatgct tggtaattg 
      aattaaatc aagtcggca acgatgaca gtatcaaag 
      atttggtgt taagaagcg cctctcaag tgacttatt 
      ctttaattt tgcattttt cgactatgg ccaacgtaa 
      catagtaaa tgaacaccc gtggggacg aaccccgac 
      ccaggggta ctttctgtg gaaaaagct caactcttc 
      aagagaaag ttgcccaat tttattacg cacatggtc 
      tgttttgta gcacatatc ccggggctt tggtcttcg 
      tcgcaattt gtgcaactc tgctctctt gttatccat 
      tattaaatt tgcctctcc ctggaaact cgcctttgc 
      aaacatgga catcgagcg tgaattcca gctcgaatg 
      tgccacctt tttgctaca ttcagcaag ttgtgctga 
      agagcacgg tcctgccgt ggggccccg agttacacc 
      taggtgacg ccttcaggg cgcctcttt acgaggctt 
      ttaaacttt tggaagctg ttagaaatt accaggatg 
      agtccacga agaaaacgc tgggacatg tgtgcttac 
      atgtcgagt cgattagga aaagtaaat caaactaca 
      aaaacatcc tgccggagt atagttgtt tagtcctgt 
      cgccggcta acttgtggc aacaggacg tcctcaaat 
      ttacgcagg atagctctc tacttacca caattttca 
      accatcata acttccaaa aaggcaaaa catcaatca 
      cggaaatga tgttcggta tgaaggtgt ttgacaccg 
      ggattaaac gatatccgt tgcgaaacc gctacagaa 
      tcattcagt acgccataa cactttttt tcaaggaat 
      atgacgaaa agtttgttc atggtttgc gcagaaagc 
      tgcagtcgg tgtattcct tacaccaaa atttggaaa 
      acggaacaa atgctaact ttgccacgc tcaacgaca 
      ctctcataa tccaatatc ccggtgaaa tcaacttgg 
      tcgagttca ttcccatta ctttggcgt ttttaatcc 
      tgttcctgt acttttatt tctcgtttc gcaaaatcg 
      aattaattc actcgaagc tcggaatgg cctttactt 
      ccgttctga ccgtgtgtg caagcctca caatgacct 
      acggcttgt gttttttct tcggcctaa ctctgaacc 
      ttgaaggaa aagttgtat ttccatatt tagtgttgc 
      tagttggct tggaaaatg tcctatgtt taacaaaaa 
      gcatagttt cgtcgcccc tccacctcg acgtgaaaa 
      tccgcatag cgcagggtt tgagcaaag aaggctggt 
      ccatcacca aaaacctcc tcctggaaa tcacgattt 
      tgacgttcg ggatcttca ccttcgtcg cggtcatcc 
      aggagtttt gcatttttg cggtcgttg gtctcgtga 
      ccgactatt tcatgcaga tgattttct tctcgacgg 
      ttttgggga ttgacatcc ggcggaagg agaacatgg 
      tggagagac acaatctga gctttcgga gcagtctct 
      tccaaagcc aggttttct acgataagt cgttcggta 
      ttcggcgat cgaagtcgc acccgcgac tgtcctcct 
      tccctgcca atatcgtgg cagggcagg tgcagggcg 
      cccaagaga aaaaaatcc cgaaacggt tgaaacttg 
      tgaatattt ggaccagga cgcggtttt tccaccgcg 
      cttttatga tcttgatgg tgatttgga tttcgaata 
      tcgcctaca atagcggct tcatcgagc attttatcc 
      tgaataaca gttattttc acgaggtgg ccgaacaaa 
      atagcacta gcttggagc agaactact cgagtatca 
      agctgtttc cgttgctga acacgaata attcgttca 
      taaacaaag tgagttatg ttactcaca gcccgctca 
      attacaatc agaaccaga cgttcgatt tggagaatt 
      tgaagcatt cggattacg agccgtggt tgaaattgt 
      cgcctcaac attggtcgt ctattgtgc gaaaataca 
      acttgtcgt tgagtggac aatacattg ccattttgc 
      caaatatta agctatcgc acgtgatac gtccagagc 
      aggcggtgc ttttgcgca accctaacg aaaaatacg 
      gggagaagt tccagtggc aggctttgg tggccgccc 
      tggacccca ttgacttca atgggatgg agacaggca 
      ccaggtctg ccatacctc agtccgagc agcccttct 
      acccgacct cagttttcg cgatgattt tgatcgcga 
      ttctgtgct ttatcagcg tggtggtgc tggggtaca 
      cctttgtgg tgctttacc ggtgcatat gctcccgga 
      actacgccg aatggcggg cgtcgtggt tcagcgaaa 
      agggggaag aaaacgtgg aagagccag ttttgttgg 
      aggcggtgc cggtggtgc tggaagggc accaacagg 
      agatcgaat gcacggcca cagacgggg ttaatttgg 
      tcagtgcct cgctagacg ggcaactga aggtttggg 
      atagtggga ccggggagc tgatcgcca atgtggaca 
      ggaaggcgt actttcagg ggtgtttcg agtcgaatt 
      gtggcactt ccgagtgcg atgataact cgtcggatt 
      atgagtccg gctctccgc cgtcccgag gggagagct 
      atctgtttc cccgtctca aacatcgga ttaatacga 
      acttttcaa acgtgaaag aacagtgta actcgtata 
      atgccaaag ctgattttt taaaaactt atcgagagt 
      tgtattttg ttcgcgatt taccgaaag ctaaaagtc 
      ggaaagaga tgaacaggg ccaaagaaa ataaatcga 
      ttagtttta gtgaaagta atcaaaagt tttgtgata 
      atggacaca cgaaatttg atagtagtg atgctaaac 
      tttcaccga tttggtgta tggactatc tggagaact 
      tgattgttc tggggtgtg ctgatggga ggctggagt 
      tttgggagg gcagttcgg ggaggttga agtgcatat 
      ttgaagacg gaaccgatg ttggaataa gcaatttaa 
      aaatcgtgg ggtcgcgaa ttatcgcat cccgattgt 
      atggcacct tggaaatgt tccaattac aaacttaca 
      accgtggcc atccagttg actggaatt tcacttgcg 
      cataccgaa gaacccacg taaggactg gctcagttg 
      gttccgtcc ctgatcaca ccaatttaa tttcacaaa 
      acgaggatg aggaagatt tccggtgtt tgaaagttt 
      tcagcgtta aggcacacc aacagccga ttatctgcc 
      tcgactgtg aaggcggca gaattttaa ccggcagcc 
      aagaccaca cgcttaaag tgttccgac tggaggacg 
      gcagccccc agtacacgc tgcacggcc actgcggcc 
      ccatcacct gtcttttca ttgacagga tcagtccag 
      cgacttctg gcagtggct cccaggacg gaatgctct 
      gcgtttggg atcttctaa ctggtgcct gtatgtaca 
      acatccagg cccatgatg gtacgataa catcgctga 
      cgtactctg cgagttacg tgatttcac tgggttctg 
      acgaccgca tttgtgttt gggagcgct tccaaggcc 
      atcttctga acaccattt acgtttctc agacctttt 
      ctagccagg tgttgatgt tggcccctc atttggttg 
      tcacagctc gcagtggag ggctagtta tttgggatg 
      tgaggtcgg gtgaatgtg tccgaacga ttatattag 
      gccgttcac cgtatattt ttatcaaac agttgatgt 
      tgttacgcg atgctgtgg tctgtgatt acggaaatc 
      aattgcgca tcgtgaggt tccctctta tcactcata 
      aatttgact aggttgttt ctacgtgcc ttcaatgat 
      gtaacatag tttcgtcta tttatgtct tgttttatt 
      tttatgtcc tttacgtaa aagcgtcaa aacgtactg 
      taaattttt cactgacaa aaggtttcg aatacctaa 
      ttgtttttt cattaagtt atttattta tttggcgga 
      aataaatgc atttaacat ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY