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Gene information
General information
TC013432 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9733119..9737868
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013432
    iBeetle screen iB_05434

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    TR: 173053 potentially confused/crosscontaminated with : potentially confused/contaminated with, iB # (comma separated) iB_05445
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013432 RA
      MEVWNCPEK VQNQNENSF MLNNDFIDD TFDFSFNNV 
      YEKATENLT KYDDKEKNS TSLVKLEEN DENAGYIHH 
      TVSLDEIYM HIHPGASKK MPEKPSHAT LTITSTDPT 
      TKETTVNRY NCEYDGCTR TYSTVGNLR THMKTHKGE 
      YRFKCTESN CGKAFLTSY SLKIHIRVH TKVKPFECK 
      SPGCDKAFN TLYRLRAHE RLHNGKTFN CESEGCMKF 
      FTTLCDLKK HTRTHTREK PYKCKEDGC GKAFTASHH 
      LKTHQRIHS GEKPYICKE SSCSRAFAT LHSLKSHIK 
      THQKSELKL EGQEEKDDS VKPELGENE RRNNDINVP 
      FDFEGSYTI GSFGSALDT WDDLEKAES KKSDVTTKQ 
      VPEPNNLTE SNLEPVNFD NLFKNSYNL VNNNYIARN 
      PDASLPPLT LDNKAKYAA VIEADLSSQ FEMANGLKN 
      YATVNTAEP IPTQLPYNI GTENIENGK AGETLNDTQ 
      MELEDSSII TEIEDAGIN FDVDMFDNV FNDDKNKKV 
      NVISVKKIV PPENDLVNL DKQIYTPEA LQMSLACDE 
      EVPSMWVDV MNYYNSGQA NVFEQNTID DSQVIAVPT 
      AVQSYVNLP PLQTTDNQL DSTYNQLNS FLMQNLEQT 
      TNTDANLLK NLTAEADIC KCVDCKCDS VNNCQNCEG 
      HDRAEAPQA QNCQSKVSC CSKPVKNLG CGGDKNDCC 
      VVVCLKTLD QLRQILNMA STCGGFQNL TLGCIKGGQ 
      FCAVQK
    CDS sequence
    • >TC013432 RA
      atggaggtc tggaactgc cccgaaaaa gtgcaaaat 
      caaaatgaa aattcattc atgttgaac aacgatttt 
      atcgacgac actttcgac tttagtttt aataatgtg 
      tacgaaaaa gcgaccgaa aaccttacg aaatatgac 
      gataaagag aaaaattcg acaagtctt gttaaattg 
      gaagaaaac gatgaaaat gccggttac atccatcat 
      acggtttct ttggacgaa atttacatg cacatccat 
      ccgggggct tccaagaaa atgccggaa aaaccatcc 
      catgccacc ttgacaatc acaagtaca gaccccaca 
      accaaagaa accacagta aaccgttac aattgcgaa 
      tacgacggt tgcactcga acctacagc actgttggt 
      aacctgcga acacacatg aaaacccac aaaggtgaa 
      tacaggttt aaatgtaca gaatccaac tgcggcaag 
      gcctttttg acctcatac agtctaaaa atacatatc 
      agagttcac accaaagtg aagcctttt gaatgcaaa 
      agccccggt tgtgataaa gcgttcaac actttgtac 
      cggttaagg gcccacgag cggttacat aacggcaaa 
      acgtttaat tgtgagtcc gaaggctgc atgaagttt 
      tttaccaca ctgtgtgac ttaaagaaa cacactcgt 
      actcacacc cgtgaaaaa ccttacaaa tgtaaggag 
      gacgggtgt gggaaagcg tttacagca agccaccac 
      ctgaagacc catcagagg atccacagt ggggagaag 
      ccctacatt tgtaaagag tcaagttgc agtcgggct 
      tttgcaact cttcatagc ctcaagtcg cacataaag 
      acccaccag aagtccgaa cttaagttg gaaggtcag 
      gaggaaaag gacgattca gtcaaacct gagcttggg 
      gaaaatgaa cggcgaaat aatgatatc aatgtgcct 
      tttgatttt gagggaagc tacacgatc gggagtttc 
      ggaagtgcc ttagatacc tgggatgac ttggagaaa 
      gctgagtcc aagaaaagt gatgtcacc acgaaacaa 
      gtacctgaa cctaacaat ctaaccgaa tccaaccta 
      gaaccagtc aatttcgac aacctattt aaaaactcg 
      tacaaccta gtcaacaac aactacatc gctcgaaac 
      cccgacgcc agtctccca cccctaaca ttagacaac 
      aaagccaaa tacgccgct gtcatcgaa gccgaccta 
      agttcccag ttcgaaatg gccaacggt ttgaaaaat 
      tacgcgacg gtcaacact gccgaacca attcccacc 
      caacttcct tacaacatt ggcaccgag aacatcgag 
      aacggcaaa gctggagag accctaaac gacacccaa 
      atggaactc gaagacagt tccataata acagaaatc 
      gaagacgca gggataaat tttgacgtt gatatgttc 
      gataatgtg ttcaacgac gataaaaat aaaaaagtc 
      aacgtaata agcgtgaaa aaaatcgtg cctccggag 
      aatgatcta gtgaacttg gacaagcag atttacact 
      ccggaggcc cttcagatg tcgctggcg tgcgacgaa 
      gaagtgcct tcgatgtgg gtggacgtc atgaactac 
      tacaactcg ggccaagcc aatgtcttt gaacagaac 
      acaatcgac gacagtcaa gtcatcgct gtaccgacg 
      gccgtccaa tcatacgtc aatctcccc ccactccaa 
      acaactgac aatcagctc gacagcacc tacaaccaa 
      ttgaacagc ttcctgatg cagaatctg gaacaaacg 
      actaacacc gacgccaac ttgctgaag aatttaaca 
      gccgaggct gacatttgc aaatgtgtg gactgcaaa 
      tgcgattcg gtcaataat tgtcaaaat tgcgaaggt 
      cacgatcgg gctgaggcg ccccaagct caaaactgc 
      caatctaag gtcagttgc tgcagcaag ccagtgaag 
      aatctaggg tgtggaggg gacaaaaat gattgttgt 
      gtcgtcgtt tgtttgaag actttggac cagttgagg 
      caaattctg aatatggcg agtacttgc ggagggttt 
      cagaattta acgctgggg tgcatcaaa ggggggcag 
      ttttgcgcc gtgcaaaag tag
    mRNA sequence
    • >TC013432 RA
      ccaatttac ttaacttaa aattaaaat acctccttg 
      tttttgttt ttgtatgtc gacattggg atgtgtaat 
      gtggtagtt tttattttt ttcacacca tggaggtct 
      ggaactgcc ccgaaaaag tgcaaaatc aaaatgaaa 
      attcattca tgttgaaca acgatttta tcgacgaca 
      ctttcgact ttagtttta ataatgtgt acgaaaaag 
      cgaccgaaa accttacga aatatgacg ataaagaga 
      aaaattcga caagtcttg ttaaattgg aagaaaacg 
      atgaaaatg ccggttaca tccatcata cggtttctt 
      tggacgaaa tttacatgc acatccatc cgggggctt 
      ccaagaaaa tgccggaaa aaccatccc atgccacct 
      tgacaatca caagtacag accccacaa ccaaagaaa 
      ccacagtaa accgttaca attgcgaat acgacggtt 
      gcactcgaa cctacagca ctgttggta acctgcgaa 
      cacacatga aaacccaca aaggtgaat acaggttta 
      aatgtacag aatccaact gcggcaagg cctttttga 
      cctcataca gtctaaaaa tacatatca gagttcaca 
      ccaaagtga agccttttg aatgcaaaa gccccggtt 
      gtgataaag cgttcaaca ctttgtacc ggttaaggg 
      cccacgagc ggttacata acggcaaaa cgtttaatt 
      gtgagtccg aaggctgca tgaagtttt ttaccacac 
      tgtgtgact taaagaaac acactcgta ctcacaccc 
      gtgaaaaac cttacaaat gtaaggagg acgggtgtg 
      ggaaagcgt ttacagcaa gccaccacc tgaagaccc 
      atcagagga tccacagtg gggagaagc cctacattt 
      gtaaagagt caagttgca gtcgggctt ttgcaactc 
      ttcatagcc tcaagtcgc acataaaga cccaccaga 
      agtccgaac ttaagttgg aaggtcagg aggaaaagg 
      acgattcag tcaaacctg agcttgggg aaaatgaac 
      ggcgaaata atgatatca atgtgcctt ttgattttg 
      agggaagct acacgatcg ggagtttcg gaagtgcct 
      tagatacct gggatgact tggagaaag ctgagtcca 
      agaaaagtg atgtcacca cgaaacaag tacctgaac 
      ctaacaatc taaccgaat ccaacctag aaccagtca 
      atttcgaca acctattta aaaactcgt acaacctag 
      tcaacaaca actacatcg ctcgaaacc ccgacgcca 
      gtctcccac ccctaacat tagacaaca aagccaaat 
      acgccgctg tcatcgaag ccgacctaa gttcccagt 
      tcgaaatgg ccaacggtt tgaaaaatt acgcgacgg 
      tcaacactg ccgaaccaa ttcccaccc aacttcctt 
      acaacattg gcaccgaga acatcgaga acggcaaag 
      ctggagaga ccctaaacg acacccaaa tggaactcg 
      aagacagtt ccataataa cagaaatcg aagacgcag 
      ggataaatt ttgacgttg atatgttcg ataatgtgt 
      tcaacgacg ataaaaata aaaaagtca acgtaataa 
      gcgtgaaaa aaatcgtgc ctccggaga atgatctag 
      tgaacttgg acaagcaga tttacactc cggaggccc 
      ttcagatgt cgctggcgt gcgacgaag aagtgcctt 
      cgatgtggg tggacgtca tgaactact acaactcgg 
      gccaagcca atgtctttg aacagaaca caatcgacg 
      acagtcaag tcatcgctg taccgacgg ccgtccaat 
      catacgtca atctccccc cactccaaa caactgaca 
      atcagctcg acagcacct acaaccaat tgaacagct 
      tcctgatgc agaatctgg aacaaacga ctaacaccg 
      acgccaact tgctgaaga atttaacag ccgaggctg 
      acatttgca aatgtgtgg actgcaaat gcgattcgg 
      tcaataatt gtcaaaatt gcgaaggtc acgatcggg 
      ctgaggcgc cccaagctc aaaactgcc aatctaagg 
      tcagttgct gcagcaagc cagtgaaga atctagggt 
      gtggagggg acaaaaatg attgttgtg tcgtcgttt 
      gtttgaaga ctttggacc agttgaggc aaattctga 
      atatggcga gtacttgcg gagggtttc agaatttaa 
      cgctggggt gcatcaaag gggggcagt tttgcgccg 
      tgcaaaagt agaccgctt aagccgaat taacgtagc 
      attgtgatt ggatagggc caaagtgct gttaacttt 
      agatgtact aatttccgt gtgatgtgc caaaaatag 
      tgtagtgca ataatatta cgttgtgat atttacaat 
      tttttaaca aattatgta actttttta agctagtgt 
      agtcgagtg cagctgggg cagacgtaa gggcgcgcc 
      gttgcttct gccgcgcgc tgtacaagt attcctcaa 
      cttcctcga taattgtaa ttgtaatac ttgcgcgta 
      ctaatgaga gagcagccg tgaagttta cattctagt 
      cattttact ttaattctc ctgttactt attgccctt 
      caaggctta tgtatggta aaagttgaa tacatattc 
      ttaataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY