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Gene information
General information
TC013432 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9733119..9737868
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013432
    iBeetle screen iB_05434

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    TR: 173053 potentially confused/crosscontaminated with : potentially confused/contaminated with, iB # (comma separated) iB_05445
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013432 RA
      MEVWNCPEK VQNQNENSF MLNNDFIDD TFDFSFNNV 
YEKATENLT KYDDKEKNS TSLVKLEEN DENAGYIHH 
TVSLDEIYM HIHPGASKK MPEKPSHAT LTITSTDPT 
TKETTVNRY NCEYDGCTR TYSTVGNLR THMKTHKGE 
YRFKCTESN CGKAFLTSY SLKIHIRVH TKVKPFECK 
SPGCDKAFN TLYRLRAHE RLHNGKTFN CESEGCMKF 
FTTLCDLKK HTRTHTREK PYKCKEDGC GKAFTASHH 
LKTHQRIHS GEKPYICKE SSCSRAFAT LHSLKSHIK 
THQKSELKL EGQEEKDDS VKPELGENE RRNNDINVP 
FDFEGSYTI GSFGSALDT WDDLEKAES KKSDVTTKQ 
VPEPNNLTE SNLEPVNFD NLFKNSYNL VNNNYIARN 
PDASLPPLT LDNKAKYAA VIEADLSSQ FEMANGLKN 
YATVNTAEP IPTQLPYNI GTENIENGK AGETLNDTQ 
MELEDSSII TEIEDAGIN FDVDMFDNV FNDDKNKKV 
NVISVKKIV PPENDLVNL DKQIYTPEA LQMSLACDE 
EVPSMWVDV MNYYNSGQA NVFEQNTID DSQVIAVPT 
AVQSYVNLP PLQTTDNQL DSTYNQLNS FLMQNLEQT 
TNTDANLLK NLTAEADIC KCVDCKCDS VNNCQNCEG 
HDRAEAPQA QNCQSKVSC CSKPVKNLG CGGDKNDCC 
VVVCLKTLD QLRQILNMA STCGGFQNL TLGCIKGGQ 
FCAVQK
    CDS sequence
    • >TC013432 RA
      atggaggtc tggaactgc cccgaaaaa gtgcaaaat 
caaaatgaa aattcattc atgttgaac aacgatttt 
atcgacgac actttcgac tttagtttt aataatgtg 
tacgaaaaa gcgaccgaa aaccttacg aaatatgac 
gataaagag aaaaattcg acaagtctt gttaaattg 
gaagaaaac gatgaaaat gccggttac atccatcat 
acggtttct ttggacgaa atttacatg cacatccat 
ccgggggct tccaagaaa atgccggaa aaaccatcc 
catgccacc ttgacaatc acaagtaca gaccccaca 
accaaagaa accacagta aaccgttac aattgcgaa 
tacgacggt tgcactcga acctacagc actgttggt 
aacctgcga acacacatg aaaacccac aaaggtgaa 
tacaggttt aaatgtaca gaatccaac tgcggcaag 
gcctttttg acctcatac agtctaaaa atacatatc 
agagttcac accaaagtg aagcctttt gaatgcaaa 
agccccggt tgtgataaa gcgttcaac actttgtac 
cggttaagg gcccacgag cggttacat aacggcaaa 
acgtttaat tgtgagtcc gaaggctgc atgaagttt 
tttaccaca ctgtgtgac ttaaagaaa cacactcgt 
actcacacc cgtgaaaaa ccttacaaa tgtaaggag 
gacgggtgt gggaaagcg tttacagca agccaccac 
ctgaagacc catcagagg atccacagt ggggagaag 
ccctacatt tgtaaagag tcaagttgc agtcgggct 
tttgcaact cttcatagc ctcaagtcg cacataaag 
acccaccag aagtccgaa cttaagttg gaaggtcag 
gaggaaaag gacgattca gtcaaacct gagcttggg 
gaaaatgaa cggcgaaat aatgatatc aatgtgcct 
tttgatttt gagggaagc tacacgatc gggagtttc 
ggaagtgcc ttagatacc tgggatgac ttggagaaa 
gctgagtcc aagaaaagt gatgtcacc acgaaacaa 
gtacctgaa cctaacaat ctaaccgaa tccaaccta 
gaaccagtc aatttcgac aacctattt aaaaactcg 
tacaaccta gtcaacaac aactacatc gctcgaaac 
cccgacgcc agtctccca cccctaaca ttagacaac 
aaagccaaa tacgccgct gtcatcgaa gccgaccta 
agttcccag ttcgaaatg gccaacggt ttgaaaaat 
tacgcgacg gtcaacact gccgaacca attcccacc 
caacttcct tacaacatt ggcaccgag aacatcgag 
aacggcaaa gctggagag accctaaac gacacccaa 
atggaactc gaagacagt tccataata acagaaatc 
gaagacgca gggataaat tttgacgtt gatatgttc 
gataatgtg ttcaacgac gataaaaat aaaaaagtc 
aacgtaata agcgtgaaa aaaatcgtg cctccggag 
aatgatcta gtgaacttg gacaagcag atttacact 
ccggaggcc cttcagatg tcgctggcg tgcgacgaa 
gaagtgcct tcgatgtgg gtggacgtc atgaactac 
tacaactcg ggccaagcc aatgtcttt gaacagaac 
acaatcgac gacagtcaa gtcatcgct gtaccgacg 
gccgtccaa tcatacgtc aatctcccc ccactccaa 
acaactgac aatcagctc gacagcacc tacaaccaa 
ttgaacagc ttcctgatg cagaatctg gaacaaacg 
actaacacc gacgccaac ttgctgaag aatttaaca 
gccgaggct gacatttgc aaatgtgtg gactgcaaa 
tgcgattcg gtcaataat tgtcaaaat tgcgaaggt 
cacgatcgg gctgaggcg ccccaagct caaaactgc 
caatctaag gtcagttgc tgcagcaag ccagtgaag 
aatctaggg tgtggaggg gacaaaaat gattgttgt 
gtcgtcgtt tgtttgaag actttggac cagttgagg 
caaattctg aatatggcg agtacttgc ggagggttt 
cagaattta acgctgggg tgcatcaaa ggggggcag 
ttttgcgcc gtgcaaaag tag
    mRNA sequence
    • >TC013432 RA
      ccaatttac ttaacttaa aattaaaat acctccttg 
tttttgttt ttgtatgtc gacattggg atgtgtaat 
gtggtagtt tttattttt ttcacacca tggaggtct 
ggaactgcc ccgaaaaag tgcaaaatc aaaatgaaa 
attcattca tgttgaaca acgatttta tcgacgaca 
ctttcgact ttagtttta ataatgtgt acgaaaaag 
cgaccgaaa accttacga aatatgacg ataaagaga 
aaaattcga caagtcttg ttaaattgg aagaaaacg 
atgaaaatg ccggttaca tccatcata cggtttctt 
tggacgaaa tttacatgc acatccatc cgggggctt 
ccaagaaaa tgccggaaa aaccatccc atgccacct 
tgacaatca caagtacag accccacaa ccaaagaaa 
ccacagtaa accgttaca attgcgaat acgacggtt 
gcactcgaa cctacagca ctgttggta acctgcgaa 
cacacatga aaacccaca aaggtgaat acaggttta 
aatgtacag aatccaact gcggcaagg cctttttga 
cctcataca gtctaaaaa tacatatca gagttcaca 
ccaaagtga agccttttg aatgcaaaa gccccggtt 
gtgataaag cgttcaaca ctttgtacc ggttaaggg 
cccacgagc ggttacata acggcaaaa cgtttaatt 
gtgagtccg aaggctgca tgaagtttt ttaccacac 
tgtgtgact taaagaaac acactcgta ctcacaccc 
gtgaaaaac cttacaaat gtaaggagg acgggtgtg 
ggaaagcgt ttacagcaa gccaccacc tgaagaccc 
atcagagga tccacagtg gggagaagc cctacattt 
gtaaagagt caagttgca gtcgggctt ttgcaactc 
ttcatagcc tcaagtcgc acataaaga cccaccaga 
agtccgaac ttaagttgg aaggtcagg aggaaaagg 
acgattcag tcaaacctg agcttgggg aaaatgaac 
ggcgaaata atgatatca atgtgcctt ttgattttg 
agggaagct acacgatcg ggagtttcg gaagtgcct 
tagatacct gggatgact tggagaaag ctgagtcca 
agaaaagtg atgtcacca cgaaacaag tacctgaac 
ctaacaatc taaccgaat ccaacctag aaccagtca 
atttcgaca acctattta aaaactcgt acaacctag 
tcaacaaca actacatcg ctcgaaacc ccgacgcca 
gtctcccac ccctaacat tagacaaca aagccaaat 
acgccgctg tcatcgaag ccgacctaa gttcccagt 
tcgaaatgg ccaacggtt tgaaaaatt acgcgacgg 
tcaacactg ccgaaccaa ttcccaccc aacttcctt 
acaacattg gcaccgaga acatcgaga acggcaaag 
ctggagaga ccctaaacg acacccaaa tggaactcg 
aagacagtt ccataataa cagaaatcg aagacgcag 
ggataaatt ttgacgttg atatgttcg ataatgtgt 
tcaacgacg ataaaaata aaaaagtca acgtaataa 
gcgtgaaaa aaatcgtgc ctccggaga atgatctag 
tgaacttgg acaagcaga tttacactc cggaggccc 
ttcagatgt cgctggcgt gcgacgaag aagtgcctt 
cgatgtggg tggacgtca tgaactact acaactcgg 
gccaagcca atgtctttg aacagaaca caatcgacg 
acagtcaag tcatcgctg taccgacgg ccgtccaat 
catacgtca atctccccc cactccaaa caactgaca 
atcagctcg acagcacct acaaccaat tgaacagct 
tcctgatgc agaatctgg aacaaacga ctaacaccg 
acgccaact tgctgaaga atttaacag ccgaggctg 
acatttgca aatgtgtgg actgcaaat gcgattcgg 
tcaataatt gtcaaaatt gcgaaggtc acgatcggg 
ctgaggcgc cccaagctc aaaactgcc aatctaagg 
tcagttgct gcagcaagc cagtgaaga atctagggt 
gtggagggg acaaaaatg attgttgtg tcgtcgttt 
gtttgaaga ctttggacc agttgaggc aaattctga 
atatggcga gtacttgcg gagggtttc agaatttaa 
cgctggggt gcatcaaag gggggcagt tttgcgccg 
tgcaaaagt agaccgctt aagccgaat taacgtagc 
attgtgatt ggatagggc caaagtgct gttaacttt 
agatgtact aatttccgt gtgatgtgc caaaaatag 
tgtagtgca ataatatta cgttgtgat atttacaat 
tttttaaca aattatgta actttttta agctagtgt 
agtcgagtg cagctgggg cagacgtaa gggcgcgcc 
gttgcttct gccgcgcgc tgtacaagt attcctcaa 
cttcctcga taattgtaa ttgtaatac ttgcgcgta 
ctaatgaga gagcagccg tgaagttta cattctagt 
cattttact ttaattctc ctgttactt attgccctt 
caaggctta tgtatggta aaagttgaa tacatattc 
ttaataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY