Status
Please wait ...
Gene information
General information
TC033148
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013420
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9900131..9902208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033148
    iBeetle screen iB_05432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033148 RA
      MFCKTSIWL LIFSSYIIE INGDQNNYK NSVQWSSIG 
      RDVIAGIRD ELSKISSNL DRNKIFSGG TTEGFLSKI 
      RLIFPGTLW CGDGNIADS SKELGKLKS TDSCCRAHD 
      MCPDDIPAG QSKHGLVNN GLFTRSHCD CDQEFYNCL 
      KNVNTVVSN GIGFTYFTV LGPQCFRQD YPIIGCLKK 
      DIRGRCLEY KTNETQEKI YEWFDNPQY HQWVLLVKC 
      FITLSSGVF LISTVALFA QNFLKGKSN PSSVFRLET 
      SQEKISTIV RNSLNHRRL LSIREKFIV PGTKWCGVG 
      DIATSYQDL GRFKEADTC CREHDSCLE YISMRDSFH 
      GITNKNLFT VSHCDCDEK FRKCLKSDG NLFSEIVGY 
      GYFTLVGPQ CFREDYPIV RCKEHVYIQ PTSKKRATK 
      RCLDYELNE SLPKEYQLF DNRPF
    CDS sequence
    • >TC033148 RA
      atgttttgc aagacgtcc atatggctc ctcatattt 
      agctcatat attatagaa ataaatggt gaccaaaac 
      aattacaaa aacagtgtt caatggagt tccataggt 
      cgagacgtg atagcagga atccgagat gagttgagc 
      aaaatttct tctaattta gatcgcaac aaaatattc 
      tctggtgga accacggag ggatttttg agtaaaata 
      cggctgatt ttcccaggc actctttgg tgcggtgac 
      ggaaacatt gccgactct agcaaggag ctgggtaaa 
      ttgaaatct actgactct tgttgcaga gcccacgac 
      atgtgtcca gacgatata ccagctgga cagtcgaaa 
      catggattg gttaacaat ggattattt acgcggtcg 
      cattgtgat tgtgatcag gaattttac aattgcctc 
      aaaaatgtc aacactgtt gtctctaat ggaatagga 
      ttcacgtac tttacagtt ttggggccc caatgcttc 
      aggcaagat tatcctata atcggttgc ctaaaaaaa 
      gatatcaga ggcaggtgc ttggaatac aaaacaaac 
      gaaactcag gagaaaatt tatgaatgg tttgataat 
      ccacaatat catcaatgg gtgttgctt gtaaaatgt 
      tttataact ttgagcagt ggagtgttc ctgatatcc 
      actgtagct ttatttgca cagaacttt cttaaaggt 
      aaaagtaat ccaagttct gtatttcgt ctagaaaca 
      agccaagaa aaaatttcc acaatcgtt agaaattcc 
      ttgaatcat aggcgttta ttaagcatt agagaaaaa 
      ttcatcgta ccagggact aaatggtgt ggtgttgga 
      gacattgca acttcatat caagattta ggtcgtttc 
      aaagaagct gacacttgc tgcagggaa catgactct 
      tgcttggaa tacatttct atgagagat tcgtttcat 
      ggaatcacc aacaagaat ctcttcact gtctcacac 
      tgcgactgt gatgaaaaa tttcgaaag tgtttgaaa 
      tccgatggc aatttgttt agtgaaatt gtagggtat 
      gggtatttc acccttgta gggccacag tgttttagg 
      gaagattat ccaatagtc cggtgcaag gaacacgtc 
      tacattcaa ccaacaagt aaaaaacgt gcaactaaa 
      aggtgcttg gactacgaa ttgaatgaa agtttgcca 
      aaggagtat cagttgttt gacaatcgg cctttttga
    mRNA sequence
    • >TC033148 RA
      ttttaaaaa tgttttgca agacgtcca tatggctcc 
      tcatattta gctcatata ttatagaaa taaatggtg 
      accaaaaca attacaaaa acagtgttc aatggagtt 
      ccataggtc gagacgtga tagcaggaa tccgagatg 
      agttgagca aaatttctt ctaatttag atcgcaaca 
      aaatattct ctggtggaa ccacggagg gatttttga 
      gtaaaatac ggctgattt tcccaggca ctctttggt 
      gcggtgacg gaaacattg ccgactcta gcaaggagc 
      tgggtaaat tgaaatcta ctgactctt gttgcagag 
      cccacgaca tgtgtccag acgatatac cagctggac 
      agtcgaaac atggattgg ttaacaatg gattattta 
      cgcggtcgc attgtgatt gtgatcagg aattttaca 
      attgcctca aaaatgtca acactgttg tctctaatg 
      gaataggat tcacgtact ttacagttt tggggcccc 
      aatgcttca ggcaagatt atcctataa tcggttgcc 
      taaaaaaag atatcagag gcaggtgct tggaataca 
      aaacaaacg aaactcagg agaaaattt atgaatggt 
      ttgataatc cacaatatc atcaatggg tgttgcttg 
      taaaatgtt ttataactt tgagcagtg gagtgttcc 
      tgatatcca ctgtagctt tatttgcac agaactttc 
      ttaaaggta aaagtaatc caagttctg tatttcgtc 
      tagaaacaa gccaagaaa aaatttcca caatcgtta 
      gaaattcct tgaatcata ggcgtttat taagcatta 
      gagaaaaat tcatcgtac cagggacta aatggtgtg 
      gtgttggag acattgcaa cttcatatc aagatttag 
      gtcgtttca aagaagctg acacttgct gcagggaac 
      atgactctt gcttggaat acatttcta tgagagatt 
      cgtttcatg gaatcacca acaagaatc tcttcactg 
      tctcacact gcgactgtg atgaaaaat ttcgaaagt 
      gtttgaaat ccgatggca atttgttta gtgaaattg 
      tagggtatg ggtatttca cccttgtag ggccacagt 
      gttttaggg aagattatc caatagtcc ggtgcaagg 
      aacacgtct acattcaac caacaagta aaaaacgtg 
      caactaaaa ggtgcttgg actacgaat tgaatgaaa 
      gtttgccaa aggagtatc agttgtttg acaatcggc 
      ctttttgag ttgctgttt tgatacttt tgtggaaaa 
      ctccaaatt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY