General information | |||
TC033149
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 9933570..9944020 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC033149 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05430 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
larva posterior decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
- >TC033149 RA
MVFLVLFFS LFLTSNALL DEGESCVVP TTKENGVCI SVHNCEHAK QLLKQGVNP QFCGFKGSN VLVCCPVSQ RVVARRQSG EKSRQQCLK LYAKKDLTL PIIFLGTKA KSNEFPHMA AIGYGDKNT TISWLCGGS LISERFILT AAHCLFSQD FGPATWVRI GDLDLKNDT DGPNPNIPI VKTFAHPKY KSSSHYHDI GLIKLDASV TFQSYSDPA CLHTDASVP VTLEAIGWG SVGYFGDQS SHLLKVGLK LVSYQKCAK RYANASKRK LKDGIVDEW QLCAGDALG GDTCPGDSG GPLHYIANE TEEMFSHFV VAGVTSFGK GCGVENSIG VYTRVAAYI DWIESKIML ELDRMSYIF GSFARRRQS QEVSSAKRR SIEPKISHD RSKSTPHTP KKAAPGSPR AKSICNVVV DDAKFKCPM CRRLFAEPK VLPCLHTFC LRCLQELEA NDYSNWAGD DSDESANTS ESRKGSGSG GSGYVSDKN NRSCSPTPV KRICCPTCG ARAEVPFGG VTCYPPNYL LQHRMVLAT LNAQSTHLL CDLCTSDVS ATARCMDCA LSFCDHCEE VHLRQKAAN GHEVLSLEE ARRKGITKV RRQIMCVKH PELELTLFC STCCQVICS DCAPFSHKG HPCEPISRA IKAHLTDLR LAASRAKSI AEQSALAAN RLHATSKKI ETQCSKVQM EIEEFIDKY IKSVEEHRK KLLQQVIQT RTEKLQEIG KCKTMLHKR VREARDVAF FLDELLSDG TDVEVMSFL KPVMTKIDK CGKRDVGKV TDLSLTGSL LFLPEEVVQ YSQDCCPLY GVVTTQTVA PSHCVISTE GLSNLRVGK KTDLLLETR DHTDTKLDR GGEQVSAEI RYRDAGVSR FLQVDIEDR RDGTYAISF VPDVAGKLV FSISIKGQP IKDSPFPIT VRTLKPHHG TFHCCSFCS SGGSKEATC GCEGRMPGG YKGCGHGHE GHPGRRHWS CCGNVLEHS ECSKPGNHS HYQFTL
- >TC033149 RA
atggttttc ttagtgtta tttttctcg ttattttta acaagcaat gctttacta gacgaaggt gagagttgt gttgttccc actacgaaa gaaaatggg gtctgtatc agtgtacac aactgcgaa catgcaaaa caacttcta aaacaaggt gtaaatccc caattttgc gggtttaaa ggaagcaac gtcttagtc tgttgtcct gttagccaa cgtgtggtg gccaggcgc cagtctggt gaaaaaagt cgccaacaa tgcttaaaa ctttacgca aaaaaggac ttgactttg cctatcata tttttaggg acaaaagcc aaatccaac gaattccca cacatggca gcgattggt tacggtgac aaaaacaca accatttcc tggttgtgt gggggcagt ttgatcagc gaacggttt atcctaacg gccgctcat tgtctcttc tcgcaagac ttcggacct gcaacctgg gtccgtatc ggcgactta gatttgaaa aacgacacc gacggtcct aaccctaac attccaatc gtcaaaact ttcgcccat cccaaatac aagtccagc tctcattac cacgatatt ggtttgatc aagttggac gcaagtgtt acttttcaa agttattcc gatcctgcg tgtttacac actgatgcc tctgttcct gtaacactt gaggctata ggatggggc agtgtcggg tatttcggc gaccaaagt agccacctt ttgaaagtc ggtttgaaa ctagtgagt taccagaag tgtgccaaa agatacgcg aatgcgtca aaaaggaaa ctgaaggat ggtattgtg gatgagtgg cagctgtgt gcaggagat gctctgggg ggagatacg tgtccgggt gattctgga ggacctctc cattatatt gcaaatgaa acagaagaa atgttcagc cattttgtt gtggcagga gttacttcc tttggtaaa gggtgtgga gtagaaaac agcattggg gtttacaca cgagtggcg gcgtatatt gattggatc gaaagcaag attatgttg gaactagac agaatgtcg tatattttc ggaagcttc gctcgccgc cgccaaagc caggaggtg tcttcggcc aaacggcgt tcaatcgag cccaaaatc agccacgac cgctccaag tccaccccc cacaccccc aagaaagcc gcccccggc agcccccgc gccaagagc atctgcaac gtggtcgtg gacgacgcc aagttcaaa tgcccgatg tgtcgccga ttgttcgcg gagcccaag gttctgccc tgcctccac accttctgc ctccgatgt ctgcaggaa ctggaggcc aacgactac tcgaattgg gccggagac gactctgac gaatctgcc aacacctct gagtcgcgc aaaggctcg ggttccggt ggctccggt tatgtgagc gacaaaaac aaccgcagc tgttccccc acgcctgtc aagcggatt tgttgcccc acgtgtggg gcacgagcg gaagtgcct ttcgggggt gtgacctgc taccctccc aactacctc ctccagcac cggatggtc ttggccaca ctcaacgct caaagcacc catcttctc tgtgatctt tgcacttcc gacgtttcg gcgacagcc cgatgcatg gactgtgct ttaagtttt tgtgaccac tgtgaagag gtgcatctg cgacagaag gcggcgaat ggtcacgaa gttttgtcg ctggaggaa gccagaagg aaagggatt acgaaggtg cggaggcaa atcatgtgt gtgaagcac ccggagctc gaattgacg ctgttttgc tccacctgt tgccaggta atatgttct gactgcgcc cccttttcc cacaaaggc cacccgtgc gagcccatt tcccgcgcc atcaaggcc cacctcacc gacctgcgg ctggccgcc tccagagcc aagtccatc gcggagcaa tcggctctg gcagccaac cgcctccac gccacctcc aagaaaatc gagacccag tgcagtaaa gtccagatg gagatcgaa gaattcatc gacaagtac atcaaatcc gtggaggaa catcgcaag aagctgctg cagcaggtg attcagacg cgcaccgaa aaactgcaa gagatcggg aagtgcaag acgatgctg cacaagcga gtgcgggaa gcaagagac gtggcgttt ttcctggac gaattgttg agtgatgga acggatgtg gaagttatg agtttcttg aaaccggtg atgactaaa attgataag tgtgggaag cgggatgtc gggaaagtg acggatttg agtttgacg gggagtttg ctgtttttg ccggaagag gtggtgcag tattcgcag gactgttgc cccttgtat ggggtggtc accacgcag acggtggcc cccagtcac tgtgttatc agcacggaa ggtttgtcc aacttacgt gttggcaaa aagacggac ctcctcctc gagacccgc gaccacacc gacacgaaa ctggaccgc ggaggcgag caagtctcc gccgaaatc cggtaccgg gacgccgga gtttcccgc ttcctccag gttgacatc gaagaccgt cgcgacggc acttacgcc atctcgttc gtgcccgac gtcgccgga aaactcgtc ttcagtatc agcatcaaa ggccaaccg atcaaagac agtcccttt ccgatcacc gttcggacg ctgaaacca caccacgga acgttccac tgctgcagc ttctgctcc agcggtggc agcaaagag gcgacgtgt gggtgcgag ggcaggatg ccgggaggg tacaaaggc tgcggtcat ggacacgag gggcatccg gggcgcagg cactggtcc tgctgcgga aacgtcctg gagcattcg gagtgctcg aagccgggg aatcactcg cattatcag tttactttg tag
- >TC033149 RA
cgttatggt tttcttagt gttattttt ctcgttatt tttaacaag caatgcttt actagacga aggtgagag ttgtgttgt tcccactac gaaagaaaa tggggtctg tatcagtgt acacaactg cgaacatgc aaaacaact tctaaaaca aggtgtaaa tccccaatt ttgcgggtt taaaggaag caacgtctt agtctgttg tcctgttag ccaacgtgt ggtggccag gcgccagtc tggtgaaaa aagtcgcca acaatgctt aaaacttta cgcaaaaaa ggacttgac tttgcctat catattttt agggacaaa agccaaatc caacgaatt cccacacat ggcagcgat tggttacgg tgacaaaaa cacaaccat ttcctggtt gtgtggggg cagtttgat cagcgaacg gtttatcct aacggccgc tcattgtct cttctcgca agacttcgg acctgcaac ctgggtccg tatcggcga cttagattt gaaaaacga caccgacgg tcctaaccc taacattcc aatcgtcaa aactttcgc ccatcccaa atacaagtc cagctctca ttaccacga tattggttt gatcaagtt ggacgcaag tgttacttt tcaaagtta ttccgatcc tgcgtgttt acacactga tgcctctgt tcctgtaac acttgaggc tataggatg gggcagtgt cgggtattt cggcgacca aagtagcca ccttttgaa agtcggttt gaaactagt gagttacca gaagtgtgc caaaagata cgcgaatgc gtcaaaaag gaaactgaa ggatggtat tgtggatga gtggcagct gtgtgcagg agatgctct ggggggaga tacgtgtcc gggtgattc tggaggacc tctccatta tattgcaaa tgaaacaga agaaatgtt cagccattt tgttgtggc aggagttac ttcctttgg taaagggtg tggagtaga aaacagcat tggggttta cacacgagt ggcggcgta tattgattg gatcgaaag caagattat gttggaact agacagaat gtcgtatat tttcggaag cttcgctcg ccgccgcca aagccagga ggtgtcttc ggccaaacg gcgttcaat cgagcccaa aatcagcca cgaccgctc caagtccac cccccacac ccccaagaa agccgcccc cggcagccc ccgcgccaa gagcatctg caacgtggt cgtggacga cgccaagtt caaatgccc gatgtgtcg ccgattgtt cgcggagcc caaggttct gccctgcct ccacacctt ctgcctccg atgtctgca ggaactgga ggccaacga ctactcgaa ttgggccgg agacgactc tgacgaatc tgccaacac ctctgagtc gcgcaaagg ctcgggttc cggtggctc cggttatgt gagcgacaa aaacaaccg cagctgttc ccccacgcc tgtcaagcg gatttgttg ccccacgtg tggggcacg agcggaagt gcctttcgg gggtgtgac ctgctaccc tcccaacta cctcctcca gcaccggat ggtcttggc cacactcaa cgctcaaag cacccatct tctctgtga tctttgcac ttccgacgt ttcggcgac agcccgatg catggactg tgctttaag tttttgtga ccactgtga agaggtgca tctgcgaca gaaggcggc gaatggtca cgaagtttt gtcgctgga ggaagccag aaggaaagg gattacgaa ggtgcggag gcaaatcat gtgtgtgaa gcacccgga gctcgaatt gacgctgtt ttgctccac ctgttgcca ggtaatatg ttctgactg cgccccctt ttcccacaa aggccaccc gtgcgagcc catttcccg cgccatcaa ggcccacct caccgacct gcggctggc cgcctccag agccaagtc catcgcgga gcaatcggc tctggcagc caaccgcct ccacgccac ctccaagaa aatcgagac ccagtgcag taaagtcca gatggagat cgaagaatt catcgacaa gtacatcaa atccgtgga ggaacatcg caagaagct gctgcagca ggtgattca gacgcgcac cgaaaaact gcaagagat cgggaagtg caagacgat gctgcacaa gcgagtgcg ggaagcaag agacgtggc gtttttcct ggacgaatt gttgagtga tggaacgga tgtggaagt tatgagttt cttgaaacc ggtgatgac taaaattga taagtgtgg gaagcggga tgtcgggaa agtgacgga tttgagttt gacggggag tttgctgtt tttgccgga agaggtggt gcagtattc gcaggactg ttgcccctt gtatggggt ggtcaccac gcagacggt ggcccccag tcactgtgt tatcagcac ggaaggttt gtccaactt acgtgttgg caaaaagac ggacctcct cctcgagac ccgcgacca caccgacac gaaactgga ccgcggagg cgagcaagt ctccgccga aatccggta ccgggacgc cggagtttc ccgcttcct ccaggttga catcgaaga ccgtcgcga cggcactta cgccatctc gttcgtgcc cgacgtcgc cggaaaact cgtcttcag tatcagcat caaaggcca accgatcaa agacagtcc ctttccgat caccgttcg gacgctgaa accacacca cggaacgtt ccactgctg cagcttctg ctccagcgg tggcagcaa agaggcgac gtgtgggtg cgagggcag gatgccggg agggtacaa aggctgcgg tcatggaca cgaggggca tccggggcg caggcactg gtcctgctg cggaaacgt cctggagca ttcggagtg ctcgaagcc ggggaatca ctcgcatta tcagtttac tttgtagaa ggcgcaagt gtaagtgta tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
