Status
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Gene information
General information
TC033149
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013417,TC013416
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9933570..9944020
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033149
    iBeetle screen iB_05430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva posterior decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033149 RA
      MVFLVLFFS LFLTSNALL DEGESCVVP TTKENGVCI 
      SVHNCEHAK QLLKQGVNP QFCGFKGSN VLVCCPVSQ 
      RVVARRQSG EKSRQQCLK LYAKKDLTL PIIFLGTKA 
      KSNEFPHMA AIGYGDKNT TISWLCGGS LISERFILT 
      AAHCLFSQD FGPATWVRI GDLDLKNDT DGPNPNIPI 
      VKTFAHPKY KSSSHYHDI GLIKLDASV TFQSYSDPA 
      CLHTDASVP VTLEAIGWG SVGYFGDQS SHLLKVGLK 
      LVSYQKCAK RYANASKRK LKDGIVDEW QLCAGDALG 
      GDTCPGDSG GPLHYIANE TEEMFSHFV VAGVTSFGK 
      GCGVENSIG VYTRVAAYI DWIESKIML ELDRMSYIF 
      GSFARRRQS QEVSSAKRR SIEPKISHD RSKSTPHTP 
      KKAAPGSPR AKSICNVVV DDAKFKCPM CRRLFAEPK 
      VLPCLHTFC LRCLQELEA NDYSNWAGD DSDESANTS 
      ESRKGSGSG GSGYVSDKN NRSCSPTPV KRICCPTCG 
      ARAEVPFGG VTCYPPNYL LQHRMVLAT LNAQSTHLL 
      CDLCTSDVS ATARCMDCA LSFCDHCEE VHLRQKAAN 
      GHEVLSLEE ARRKGITKV RRQIMCVKH PELELTLFC 
      STCCQVICS DCAPFSHKG HPCEPISRA IKAHLTDLR 
      LAASRAKSI AEQSALAAN RLHATSKKI ETQCSKVQM 
      EIEEFIDKY IKSVEEHRK KLLQQVIQT RTEKLQEIG 
      KCKTMLHKR VREARDVAF FLDELLSDG TDVEVMSFL 
      KPVMTKIDK CGKRDVGKV TDLSLTGSL LFLPEEVVQ 
      YSQDCCPLY GVVTTQTVA PSHCVISTE GLSNLRVGK 
      KTDLLLETR DHTDTKLDR GGEQVSAEI RYRDAGVSR 
      FLQVDIEDR RDGTYAISF VPDVAGKLV FSISIKGQP 
      IKDSPFPIT VRTLKPHHG TFHCCSFCS SGGSKEATC 
      GCEGRMPGG YKGCGHGHE GHPGRRHWS CCGNVLEHS 
      ECSKPGNHS HYQFTL
    CDS sequence
    • >TC033149 RA
      atggttttc ttagtgtta tttttctcg ttattttta 
      acaagcaat gctttacta gacgaaggt gagagttgt 
      gttgttccc actacgaaa gaaaatggg gtctgtatc 
      agtgtacac aactgcgaa catgcaaaa caacttcta 
      aaacaaggt gtaaatccc caattttgc gggtttaaa 
      ggaagcaac gtcttagtc tgttgtcct gttagccaa 
      cgtgtggtg gccaggcgc cagtctggt gaaaaaagt 
      cgccaacaa tgcttaaaa ctttacgca aaaaaggac 
      ttgactttg cctatcata tttttaggg acaaaagcc 
      aaatccaac gaattccca cacatggca gcgattggt 
      tacggtgac aaaaacaca accatttcc tggttgtgt 
      gggggcagt ttgatcagc gaacggttt atcctaacg 
      gccgctcat tgtctcttc tcgcaagac ttcggacct 
      gcaacctgg gtccgtatc ggcgactta gatttgaaa 
      aacgacacc gacggtcct aaccctaac attccaatc 
      gtcaaaact ttcgcccat cccaaatac aagtccagc 
      tctcattac cacgatatt ggtttgatc aagttggac 
      gcaagtgtt acttttcaa agttattcc gatcctgcg 
      tgtttacac actgatgcc tctgttcct gtaacactt 
      gaggctata ggatggggc agtgtcggg tatttcggc 
      gaccaaagt agccacctt ttgaaagtc ggtttgaaa 
      ctagtgagt taccagaag tgtgccaaa agatacgcg 
      aatgcgtca aaaaggaaa ctgaaggat ggtattgtg 
      gatgagtgg cagctgtgt gcaggagat gctctgggg 
      ggagatacg tgtccgggt gattctgga ggacctctc 
      cattatatt gcaaatgaa acagaagaa atgttcagc 
      cattttgtt gtggcagga gttacttcc tttggtaaa 
      gggtgtgga gtagaaaac agcattggg gtttacaca 
      cgagtggcg gcgtatatt gattggatc gaaagcaag 
      attatgttg gaactagac agaatgtcg tatattttc 
      ggaagcttc gctcgccgc cgccaaagc caggaggtg 
      tcttcggcc aaacggcgt tcaatcgag cccaaaatc 
      agccacgac cgctccaag tccaccccc cacaccccc 
      aagaaagcc gcccccggc agcccccgc gccaagagc 
      atctgcaac gtggtcgtg gacgacgcc aagttcaaa 
      tgcccgatg tgtcgccga ttgttcgcg gagcccaag 
      gttctgccc tgcctccac accttctgc ctccgatgt 
      ctgcaggaa ctggaggcc aacgactac tcgaattgg 
      gccggagac gactctgac gaatctgcc aacacctct 
      gagtcgcgc aaaggctcg ggttccggt ggctccggt 
      tatgtgagc gacaaaaac aaccgcagc tgttccccc 
      acgcctgtc aagcggatt tgttgcccc acgtgtggg 
      gcacgagcg gaagtgcct ttcgggggt gtgacctgc 
      taccctccc aactacctc ctccagcac cggatggtc 
      ttggccaca ctcaacgct caaagcacc catcttctc 
      tgtgatctt tgcacttcc gacgtttcg gcgacagcc 
      cgatgcatg gactgtgct ttaagtttt tgtgaccac 
      tgtgaagag gtgcatctg cgacagaag gcggcgaat 
      ggtcacgaa gttttgtcg ctggaggaa gccagaagg 
      aaagggatt acgaaggtg cggaggcaa atcatgtgt 
      gtgaagcac ccggagctc gaattgacg ctgttttgc 
      tccacctgt tgccaggta atatgttct gactgcgcc 
      cccttttcc cacaaaggc cacccgtgc gagcccatt 
      tcccgcgcc atcaaggcc cacctcacc gacctgcgg 
      ctggccgcc tccagagcc aagtccatc gcggagcaa 
      tcggctctg gcagccaac cgcctccac gccacctcc 
      aagaaaatc gagacccag tgcagtaaa gtccagatg 
      gagatcgaa gaattcatc gacaagtac atcaaatcc 
      gtggaggaa catcgcaag aagctgctg cagcaggtg 
      attcagacg cgcaccgaa aaactgcaa gagatcggg 
      aagtgcaag acgatgctg cacaagcga gtgcgggaa 
      gcaagagac gtggcgttt ttcctggac gaattgttg 
      agtgatgga acggatgtg gaagttatg agtttcttg 
      aaaccggtg atgactaaa attgataag tgtgggaag 
      cgggatgtc gggaaagtg acggatttg agtttgacg 
      gggagtttg ctgtttttg ccggaagag gtggtgcag 
      tattcgcag gactgttgc cccttgtat ggggtggtc 
      accacgcag acggtggcc cccagtcac tgtgttatc 
      agcacggaa ggtttgtcc aacttacgt gttggcaaa 
      aagacggac ctcctcctc gagacccgc gaccacacc 
      gacacgaaa ctggaccgc ggaggcgag caagtctcc 
      gccgaaatc cggtaccgg gacgccgga gtttcccgc 
      ttcctccag gttgacatc gaagaccgt cgcgacggc 
      acttacgcc atctcgttc gtgcccgac gtcgccgga 
      aaactcgtc ttcagtatc agcatcaaa ggccaaccg 
      atcaaagac agtcccttt ccgatcacc gttcggacg 
      ctgaaacca caccacgga acgttccac tgctgcagc 
      ttctgctcc agcggtggc agcaaagag gcgacgtgt 
      gggtgcgag ggcaggatg ccgggaggg tacaaaggc 
      tgcggtcat ggacacgag gggcatccg gggcgcagg 
      cactggtcc tgctgcgga aacgtcctg gagcattcg 
      gagtgctcg aagccgggg aatcactcg cattatcag 
      tttactttg tag
    mRNA sequence
    • >TC033149 RA
      cgttatggt tttcttagt gttattttt ctcgttatt 
      tttaacaag caatgcttt actagacga aggtgagag 
      ttgtgttgt tcccactac gaaagaaaa tggggtctg 
      tatcagtgt acacaactg cgaacatgc aaaacaact 
      tctaaaaca aggtgtaaa tccccaatt ttgcgggtt 
      taaaggaag caacgtctt agtctgttg tcctgttag 
      ccaacgtgt ggtggccag gcgccagtc tggtgaaaa 
      aagtcgcca acaatgctt aaaacttta cgcaaaaaa 
      ggacttgac tttgcctat catattttt agggacaaa 
      agccaaatc caacgaatt cccacacat ggcagcgat 
      tggttacgg tgacaaaaa cacaaccat ttcctggtt 
      gtgtggggg cagtttgat cagcgaacg gtttatcct 
      aacggccgc tcattgtct cttctcgca agacttcgg 
      acctgcaac ctgggtccg tatcggcga cttagattt 
      gaaaaacga caccgacgg tcctaaccc taacattcc 
      aatcgtcaa aactttcgc ccatcccaa atacaagtc 
      cagctctca ttaccacga tattggttt gatcaagtt 
      ggacgcaag tgttacttt tcaaagtta ttccgatcc 
      tgcgtgttt acacactga tgcctctgt tcctgtaac 
      acttgaggc tataggatg gggcagtgt cgggtattt 
      cggcgacca aagtagcca ccttttgaa agtcggttt 
      gaaactagt gagttacca gaagtgtgc caaaagata 
      cgcgaatgc gtcaaaaag gaaactgaa ggatggtat 
      tgtggatga gtggcagct gtgtgcagg agatgctct 
      ggggggaga tacgtgtcc gggtgattc tggaggacc 
      tctccatta tattgcaaa tgaaacaga agaaatgtt 
      cagccattt tgttgtggc aggagttac ttcctttgg 
      taaagggtg tggagtaga aaacagcat tggggttta 
      cacacgagt ggcggcgta tattgattg gatcgaaag 
      caagattat gttggaact agacagaat gtcgtatat 
      tttcggaag cttcgctcg ccgccgcca aagccagga 
      ggtgtcttc ggccaaacg gcgttcaat cgagcccaa 
      aatcagcca cgaccgctc caagtccac cccccacac 
      ccccaagaa agccgcccc cggcagccc ccgcgccaa 
      gagcatctg caacgtggt cgtggacga cgccaagtt 
      caaatgccc gatgtgtcg ccgattgtt cgcggagcc 
      caaggttct gccctgcct ccacacctt ctgcctccg 
      atgtctgca ggaactgga ggccaacga ctactcgaa 
      ttgggccgg agacgactc tgacgaatc tgccaacac 
      ctctgagtc gcgcaaagg ctcgggttc cggtggctc 
      cggttatgt gagcgacaa aaacaaccg cagctgttc 
      ccccacgcc tgtcaagcg gatttgttg ccccacgtg 
      tggggcacg agcggaagt gcctttcgg gggtgtgac 
      ctgctaccc tcccaacta cctcctcca gcaccggat 
      ggtcttggc cacactcaa cgctcaaag cacccatct 
      tctctgtga tctttgcac ttccgacgt ttcggcgac 
      agcccgatg catggactg tgctttaag tttttgtga 
      ccactgtga agaggtgca tctgcgaca gaaggcggc 
      gaatggtca cgaagtttt gtcgctgga ggaagccag 
      aaggaaagg gattacgaa ggtgcggag gcaaatcat 
      gtgtgtgaa gcacccgga gctcgaatt gacgctgtt 
      ttgctccac ctgttgcca ggtaatatg ttctgactg 
      cgccccctt ttcccacaa aggccaccc gtgcgagcc 
      catttcccg cgccatcaa ggcccacct caccgacct 
      gcggctggc cgcctccag agccaagtc catcgcgga 
      gcaatcggc tctggcagc caaccgcct ccacgccac 
      ctccaagaa aatcgagac ccagtgcag taaagtcca 
      gatggagat cgaagaatt catcgacaa gtacatcaa 
      atccgtgga ggaacatcg caagaagct gctgcagca 
      ggtgattca gacgcgcac cgaaaaact gcaagagat 
      cgggaagtg caagacgat gctgcacaa gcgagtgcg 
      ggaagcaag agacgtggc gtttttcct ggacgaatt 
      gttgagtga tggaacgga tgtggaagt tatgagttt 
      cttgaaacc ggtgatgac taaaattga taagtgtgg 
      gaagcggga tgtcgggaa agtgacgga tttgagttt 
      gacggggag tttgctgtt tttgccgga agaggtggt 
      gcagtattc gcaggactg ttgcccctt gtatggggt 
      ggtcaccac gcagacggt ggcccccag tcactgtgt 
      tatcagcac ggaaggttt gtccaactt acgtgttgg 
      caaaaagac ggacctcct cctcgagac ccgcgacca 
      caccgacac gaaactgga ccgcggagg cgagcaagt 
      ctccgccga aatccggta ccgggacgc cggagtttc 
      ccgcttcct ccaggttga catcgaaga ccgtcgcga 
      cggcactta cgccatctc gttcgtgcc cgacgtcgc 
      cggaaaact cgtcttcag tatcagcat caaaggcca 
      accgatcaa agacagtcc ctttccgat caccgttcg 
      gacgctgaa accacacca cggaacgtt ccactgctg 
      cagcttctg ctccagcgg tggcagcaa agaggcgac 
      gtgtgggtg cgagggcag gatgccggg agggtacaa 
      aggctgcgg tcatggaca cgaggggca tccggggcg 
      caggcactg gtcctgctg cggaaacgt cctggagca 
      ttcggagtg ctcgaagcc ggggaatca ctcgcatta 
      tcagtttac tttgtagaa ggcgcaagt gtaagtgta 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY