Status
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Gene information
General information
TC033149
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013417,TC013416 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9933570..9944020
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033149
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05430

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva posterior decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033149 RA
      MVFLVLFFS LFLTSNALL DEGESCVVP TTKENGVCI 
SVHNCEHAK QLLKQGVNP QFCGFKGSN VLVCCPVSQ 
RVVARRQSG EKSRQQCLK LYAKKDLTL PIIFLGTKA 
KSNEFPHMA AIGYGDKNT TISWLCGGS LISERFILT 
AAHCLFSQD FGPATWVRI GDLDLKNDT DGPNPNIPI 
VKTFAHPKY KSSSHYHDI GLIKLDASV TFQSYSDPA 
CLHTDASVP VTLEAIGWG SVGYFGDQS SHLLKVGLK 
LVSYQKCAK RYANASKRK LKDGIVDEW QLCAGDALG 
GDTCPGDSG GPLHYIANE TEEMFSHFV VAGVTSFGK 
GCGVENSIG VYTRVAAYI DWIESKIML ELDRMSYIF 
GSFARRRQS QEVSSAKRR SIEPKISHD RSKSTPHTP 
KKAAPGSPR AKSICNVVV DDAKFKCPM CRRLFAEPK 
VLPCLHTFC LRCLQELEA NDYSNWAGD DSDESANTS 
ESRKGSGSG GSGYVSDKN NRSCSPTPV KRICCPTCG 
ARAEVPFGG VTCYPPNYL LQHRMVLAT LNAQSTHLL 
CDLCTSDVS ATARCMDCA LSFCDHCEE VHLRQKAAN 
GHEVLSLEE ARRKGITKV RRQIMCVKH PELELTLFC 
STCCQVICS DCAPFSHKG HPCEPISRA IKAHLTDLR 
LAASRAKSI AEQSALAAN RLHATSKKI ETQCSKVQM 
EIEEFIDKY IKSVEEHRK KLLQQVIQT RTEKLQEIG 
KCKTMLHKR VREARDVAF FLDELLSDG TDVEVMSFL 
KPVMTKIDK CGKRDVGKV TDLSLTGSL LFLPEEVVQ 
YSQDCCPLY GVVTTQTVA PSHCVISTE GLSNLRVGK 
KTDLLLETR DHTDTKLDR GGEQVSAEI RYRDAGVSR 
FLQVDIEDR RDGTYAISF VPDVAGKLV FSISIKGQP 
IKDSPFPIT VRTLKPHHG TFHCCSFCS SGGSKEATC 
GCEGRMPGG YKGCGHGHE GHPGRRHWS CCGNVLEHS 
ECSKPGNHS HYQFTL
    CDS sequence
    • >TC033149 RA
      atggttttc ttagtgtta tttttctcg ttattttta 
acaagcaat gctttacta gacgaaggt gagagttgt 
gttgttccc actacgaaa gaaaatggg gtctgtatc 
agtgtacac aactgcgaa catgcaaaa caacttcta 
aaacaaggt gtaaatccc caattttgc gggtttaaa 
ggaagcaac gtcttagtc tgttgtcct gttagccaa 
cgtgtggtg gccaggcgc cagtctggt gaaaaaagt 
cgccaacaa tgcttaaaa ctttacgca aaaaaggac 
ttgactttg cctatcata tttttaggg acaaaagcc 
aaatccaac gaattccca cacatggca gcgattggt 
tacggtgac aaaaacaca accatttcc tggttgtgt 
gggggcagt ttgatcagc gaacggttt atcctaacg 
gccgctcat tgtctcttc tcgcaagac ttcggacct 
gcaacctgg gtccgtatc ggcgactta gatttgaaa 
aacgacacc gacggtcct aaccctaac attccaatc 
gtcaaaact ttcgcccat cccaaatac aagtccagc 
tctcattac cacgatatt ggtttgatc aagttggac 
gcaagtgtt acttttcaa agttattcc gatcctgcg 
tgtttacac actgatgcc tctgttcct gtaacactt 
gaggctata ggatggggc agtgtcggg tatttcggc 
gaccaaagt agccacctt ttgaaagtc ggtttgaaa 
ctagtgagt taccagaag tgtgccaaa agatacgcg 
aatgcgtca aaaaggaaa ctgaaggat ggtattgtg 
gatgagtgg cagctgtgt gcaggagat gctctgggg 
ggagatacg tgtccgggt gattctgga ggacctctc 
cattatatt gcaaatgaa acagaagaa atgttcagc 
cattttgtt gtggcagga gttacttcc tttggtaaa 
gggtgtgga gtagaaaac agcattggg gtttacaca 
cgagtggcg gcgtatatt gattggatc gaaagcaag 
attatgttg gaactagac agaatgtcg tatattttc 
ggaagcttc gctcgccgc cgccaaagc caggaggtg 
tcttcggcc aaacggcgt tcaatcgag cccaaaatc 
agccacgac cgctccaag tccaccccc cacaccccc 
aagaaagcc gcccccggc agcccccgc gccaagagc 
atctgcaac gtggtcgtg gacgacgcc aagttcaaa 
tgcccgatg tgtcgccga ttgttcgcg gagcccaag 
gttctgccc tgcctccac accttctgc ctccgatgt 
ctgcaggaa ctggaggcc aacgactac tcgaattgg 
gccggagac gactctgac gaatctgcc aacacctct 
gagtcgcgc aaaggctcg ggttccggt ggctccggt 
tatgtgagc gacaaaaac aaccgcagc tgttccccc 
acgcctgtc aagcggatt tgttgcccc acgtgtggg 
gcacgagcg gaagtgcct ttcgggggt gtgacctgc 
taccctccc aactacctc ctccagcac cggatggtc 
ttggccaca ctcaacgct caaagcacc catcttctc 
tgtgatctt tgcacttcc gacgtttcg gcgacagcc 
cgatgcatg gactgtgct ttaagtttt tgtgaccac 
tgtgaagag gtgcatctg cgacagaag gcggcgaat 
ggtcacgaa gttttgtcg ctggaggaa gccagaagg 
aaagggatt acgaaggtg cggaggcaa atcatgtgt 
gtgaagcac ccggagctc gaattgacg ctgttttgc 
tccacctgt tgccaggta atatgttct gactgcgcc 
cccttttcc cacaaaggc cacccgtgc gagcccatt 
tcccgcgcc atcaaggcc cacctcacc gacctgcgg 
ctggccgcc tccagagcc aagtccatc gcggagcaa 
tcggctctg gcagccaac cgcctccac gccacctcc 
aagaaaatc gagacccag tgcagtaaa gtccagatg 
gagatcgaa gaattcatc gacaagtac atcaaatcc 
gtggaggaa catcgcaag aagctgctg cagcaggtg 
attcagacg cgcaccgaa aaactgcaa gagatcggg 
aagtgcaag acgatgctg cacaagcga gtgcgggaa 
gcaagagac gtggcgttt ttcctggac gaattgttg 
agtgatgga acggatgtg gaagttatg agtttcttg 
aaaccggtg atgactaaa attgataag tgtgggaag 
cgggatgtc gggaaagtg acggatttg agtttgacg 
gggagtttg ctgtttttg ccggaagag gtggtgcag 
tattcgcag gactgttgc cccttgtat ggggtggtc 
accacgcag acggtggcc cccagtcac tgtgttatc 
agcacggaa ggtttgtcc aacttacgt gttggcaaa 
aagacggac ctcctcctc gagacccgc gaccacacc 
gacacgaaa ctggaccgc ggaggcgag caagtctcc 
gccgaaatc cggtaccgg gacgccgga gtttcccgc 
ttcctccag gttgacatc gaagaccgt cgcgacggc 
acttacgcc atctcgttc gtgcccgac gtcgccgga 
aaactcgtc ttcagtatc agcatcaaa ggccaaccg 
atcaaagac agtcccttt ccgatcacc gttcggacg 
ctgaaacca caccacgga acgttccac tgctgcagc 
ttctgctcc agcggtggc agcaaagag gcgacgtgt 
gggtgcgag ggcaggatg ccgggaggg tacaaaggc 
tgcggtcat ggacacgag gggcatccg gggcgcagg 
cactggtcc tgctgcgga aacgtcctg gagcattcg 
gagtgctcg aagccgggg aatcactcg cattatcag 
tttactttg tag
    mRNA sequence
    • >TC033149 RA
      cgttatggt tttcttagt gttattttt ctcgttatt 
tttaacaag caatgcttt actagacga aggtgagag 
ttgtgttgt tcccactac gaaagaaaa tggggtctg 
tatcagtgt acacaactg cgaacatgc aaaacaact 
tctaaaaca aggtgtaaa tccccaatt ttgcgggtt 
taaaggaag caacgtctt agtctgttg tcctgttag 
ccaacgtgt ggtggccag gcgccagtc tggtgaaaa 
aagtcgcca acaatgctt aaaacttta cgcaaaaaa 
ggacttgac tttgcctat catattttt agggacaaa 
agccaaatc caacgaatt cccacacat ggcagcgat 
tggttacgg tgacaaaaa cacaaccat ttcctggtt 
gtgtggggg cagtttgat cagcgaacg gtttatcct 
aacggccgc tcattgtct cttctcgca agacttcgg 
acctgcaac ctgggtccg tatcggcga cttagattt 
gaaaaacga caccgacgg tcctaaccc taacattcc 
aatcgtcaa aactttcgc ccatcccaa atacaagtc 
cagctctca ttaccacga tattggttt gatcaagtt 
ggacgcaag tgttacttt tcaaagtta ttccgatcc 
tgcgtgttt acacactga tgcctctgt tcctgtaac 
acttgaggc tataggatg gggcagtgt cgggtattt 
cggcgacca aagtagcca ccttttgaa agtcggttt 
gaaactagt gagttacca gaagtgtgc caaaagata 
cgcgaatgc gtcaaaaag gaaactgaa ggatggtat 
tgtggatga gtggcagct gtgtgcagg agatgctct 
ggggggaga tacgtgtcc gggtgattc tggaggacc 
tctccatta tattgcaaa tgaaacaga agaaatgtt 
cagccattt tgttgtggc aggagttac ttcctttgg 
taaagggtg tggagtaga aaacagcat tggggttta 
cacacgagt ggcggcgta tattgattg gatcgaaag 
caagattat gttggaact agacagaat gtcgtatat 
tttcggaag cttcgctcg ccgccgcca aagccagga 
ggtgtcttc ggccaaacg gcgttcaat cgagcccaa 
aatcagcca cgaccgctc caagtccac cccccacac 
ccccaagaa agccgcccc cggcagccc ccgcgccaa 
gagcatctg caacgtggt cgtggacga cgccaagtt 
caaatgccc gatgtgtcg ccgattgtt cgcggagcc 
caaggttct gccctgcct ccacacctt ctgcctccg 
atgtctgca ggaactgga ggccaacga ctactcgaa 
ttgggccgg agacgactc tgacgaatc tgccaacac 
ctctgagtc gcgcaaagg ctcgggttc cggtggctc 
cggttatgt gagcgacaa aaacaaccg cagctgttc 
ccccacgcc tgtcaagcg gatttgttg ccccacgtg 
tggggcacg agcggaagt gcctttcgg gggtgtgac 
ctgctaccc tcccaacta cctcctcca gcaccggat 
ggtcttggc cacactcaa cgctcaaag cacccatct 
tctctgtga tctttgcac ttccgacgt ttcggcgac 
agcccgatg catggactg tgctttaag tttttgtga 
ccactgtga agaggtgca tctgcgaca gaaggcggc 
gaatggtca cgaagtttt gtcgctgga ggaagccag 
aaggaaagg gattacgaa ggtgcggag gcaaatcat 
gtgtgtgaa gcacccgga gctcgaatt gacgctgtt 
ttgctccac ctgttgcca ggtaatatg ttctgactg 
cgccccctt ttcccacaa aggccaccc gtgcgagcc 
catttcccg cgccatcaa ggcccacct caccgacct 
gcggctggc cgcctccag agccaagtc catcgcgga 
gcaatcggc tctggcagc caaccgcct ccacgccac 
ctccaagaa aatcgagac ccagtgcag taaagtcca 
gatggagat cgaagaatt catcgacaa gtacatcaa 
atccgtgga ggaacatcg caagaagct gctgcagca 
ggtgattca gacgcgcac cgaaaaact gcaagagat 
cgggaagtg caagacgat gctgcacaa gcgagtgcg 
ggaagcaag agacgtggc gtttttcct ggacgaatt 
gttgagtga tggaacgga tgtggaagt tatgagttt 
cttgaaacc ggtgatgac taaaattga taagtgtgg 
gaagcggga tgtcgggaa agtgacgga tttgagttt 
gacggggag tttgctgtt tttgccgga agaggtggt 
gcagtattc gcaggactg ttgcccctt gtatggggt 
ggtcaccac gcagacggt ggcccccag tcactgtgt 
tatcagcac ggaaggttt gtccaactt acgtgttgg 
caaaaagac ggacctcct cctcgagac ccgcgacca 
caccgacac gaaactgga ccgcggagg cgagcaagt 
ctccgccga aatccggta ccgggacgc cggagtttc 
ccgcttcct ccaggttga catcgaaga ccgtcgcga 
cggcactta cgccatctc gttcgtgcc cgacgtcgc 
cggaaaact cgtcttcag tatcagcat caaaggcca 
accgatcaa agacagtcc ctttccgat caccgttcg 
gacgctgaa accacacca cggaacgtt ccactgctg 
cagcttctg ctccagcgg tggcagcaa agaggcgac 
gtgtgggtg cgagggcag gatgccggg agggtacaa 
aggctgcgg tcatggaca cgaggggca tccggggcg 
caggcactg gtcctgctg cggaaacgt cctggagca 
ttcggagtg ctcgaagcc ggggaatca ctcgcatta 
tcagtttac tttgtagaa ggcgcaagt gtaagtgta 
tt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY