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Gene information
General information
TC013383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10231715..10232895
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013383
    iBeetle screen iB_05426

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013383 RA
      MEFYDKVKR FLGFSSRQE SPQFDERDR SWPEPPSRD 
      EGFGAPFQS FSMFSDPFE MHKYFEQQM SEMLRNFGF 
      HEFGDGFNH SFDLPQLEI EEIPDDEGA PKSGTLRDQ 
      FLKPGFEKP KERIEEKRD EDIDGRLDI RDWGSVFKG 
      ENSQTPVPR TRFFGQSVT SKTVRNPDG SIETHHTVR 
      DNEGNEETT ITRKVADKE YTITKRRDK EGKEEIIEN 
      LVNLDENEK DKFFPQQRK LPELGNKPW PFFDQFFK
    CDS sequence
    • >TC013383 RA
      atggaattc tacgacaaa gttaagaga tttttgggg 
      ttttcctca cgacaggag agcccccaa ttcgacgaa 
      cgagacagg agttggcct gagccccca agtagagat 
      gagggattt ggggcgccg ttccaaagt tttagcatg 
      tttagcgac ccctttgag atgcacaaa tatttcgag 
      caacaaatg agcgaaatg ttgcgcaat tttggtttt 
      catgagttt ggggatggt tttaaccac agttttgac 
      ttgccacag cttgaaatt gaggaaatc cctgatgac 
      gaaggcgcg ccaaaatct gggacttta agggatcaa 
      ttcctcaaa cctgggttt gagaaaccc aaggagaga 
      attgaagag aaaagagat gaagacatt gacggcagg 
      ttggatata agggattgg ggatcggtt tttaagggt 
      gaaaattct caaacgcca gtaccgagg acacgcttt 
      tttggccaa agtgttacc tccaaaact gttaggaat 
      ccagatggg tctattgaa acgcatcat actgtgagg 
      gataacgag ggtaatgaa gaaacgacc atcacgcga 
      aaagtagct gataaagaa tataccatt acaaaacgt 
      agagataaa gaaggtaaa gaagaaatt attgaaaat 
      ctggtaaac ttagatgag aatgagaag gataaattt 
      ttcccgcaa caaaggaaa cttccggaa ttgggtaac 
      aaaccttgg ccgtttttt gaccagttt tttaaataa
    mRNA sequence
    • >TC013383 RA
      tcacgaatg tattgttgg ttgcatgcc acacttgac 
      gtttctgga atatttcat tagttaaaa tcgtgtgtg 
      atgtttaaa taataaaat ggaattcta cgacaaagt 
      taagagatt tttggggtt ttcctcacg acaggagag 
      cccccaatt cgacgaacg agacaggag ttggcctga 
      gcccccaag tagagatga gggatttgg ggcgccgtt 
      ccaaagttt tagcatgtt tagcgaccc ctttgagat 
      gcacaaata tttcgagca acaaatgag cgaaatgtt 
      gcgcaattt tggttttca tgagtttgg ggatggttt 
      taaccacag ttttgactt gccacagct tgaaattga 
      ggaaatccc tgatgacga aggcgcgcc aaaatctgg 
      gactttaag ggatcaatt cctcaaacc tgggtttga 
      gaaacccaa ggagagaat tgaagagaa aagagatga 
      agacattga cggcaggtt ggatataag ggattgggg 
      atcggtttt taagggtga aaattctca aacgccagt 
      accgaggac acgcttttt tggccaaag tgttacctc 
      caaaactgt taggaatcc agatgggtc tattgaaac 
      gcatcatac tgtgaggga taacgaggg taatgaaga 
      aacgaccat cacgcgaaa agtagctga taaagaata 
      taccattac aaaacgtag agataaaga aggtaaaga 
      agaaattat tgaaaatct ggtaaactt agatgagaa 
      tgagaagga taaattttt cccgcaaca aaggaaact 
      tccggaatt gggtaacaa accttggcc gttttttga 
      ccagttttt taaataatt gaattgtaa agtatgaaa 
      atagaaaaa tttatttat tgatatatt ctggacacg 
      taagcaagc attaaaaac agaaacgtt gttgaggtc 
      gttttaaga aataaaaca acttgtttt ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY