Status
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Gene information
General information
TC013380 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10256348..10258425
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013380
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05425

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013380 RA
      MNVCRVCLS DKDLKELFA VSPHNGAVN FLALVQIHK 
      NDGLPQLIC PQCLDELAT CVRFFDKCL STHAKLKNH 
      KCLKCGQIY PSHKSLTIH ISRQHTPTV IVQKNHKSG 
      QQCHICGKI FTYRVELSR HLCSHVGLR QYKCDKCGK 
      SYITKASLN KHIVKHEGL RRFECLLCS KSFLHQDNL 
      QTHLKTHSE KKYECRVCT KKFKSNRSL LMHLKIHAN 
      ERDFPCSEC EKAFVNKYD LLRHLKLHE KKRLIASNT 
      YDKTKDDLL KTKNMRQCD QCGKICSSH GDLIKHMRV 
      HSEERPFCC KNCNKGFKS ASNLRCHER IHTKVGQKA 
      LWRNNTLFV TE
    CDS sequence
    • >TC013380 RA
      atgaatgtg tgccgagtg tgtttaagt gacaaagac 
      ttgaaagag ttgttcgcc gtgagcccc cataatggt 
      gcagtaaat ttcctggcc ctggtgcag atacataaa 
      aacgacggt cttccccaa ctcatctgt cctcaatgt 
      ctcgatgaa ctcgccact tgcgttcgt tttttcgat 
      aaatgctta tcaactcat gcaaagctc aaaaaccac 
      aagtgcctc aagtgtggc caaatatat ccttcgcat 
      aaatcgcta acaatccac ataagccgc caacacact 
      cctacagtt atcgtgcaa aaaaatcac aagtcgggc 
      caacagtgt cacatttgt gggaaaatt ttcacgtac 
      agggttgaa ctgagccgg cacttgtgc tcccacgtg 
      ggcttacgg caatacaag tgcgataag tgtggcaaa 
      agctacata actaaggcc tcccttaat aagcacatc 
      gtaaaacat gaggggctg aggagattt gagtgtctc 
      ctttgtagt aaaagtttc ctccatcag gacaactta 
      caaacgcat ttaaaaaca cacagtgag aaaaaatac 
      gaatgcagg gtgtgcaca aaaaaattc aagtcaaat 
      cgttcactt ctcatgcac ttgaaaatc catgctaac 
      gagagggat tttccgtgc tccgagtgc gagaaagcg 
      ttcgtcaac aaatacgac ttgttacga catttgaaa 
      ctgcacgag aaaaaaaga ctgatcgca agtaacacg 
      tacgataag actaaagac gatttactc aaaacgaaa 
      aatatgaga cagtgcgac caatgcggg aaaatctgt 
      tcgagtcat ggcgatttg attaaacac atgagggtg 
      cactcggag gaacgaccc ttttgttgt aaaaattgc 
      aacaaaggg tttaagtca gccagtaac ttgcgatgc 
      cacgaaagg atacataca aaagtagga caaaaagca 
      ctttggaga aataatact ttatttgtt acagagtaa
    mRNA sequence
    • >TC013380 RA
      tggtgcaat caaggccgt ttggcgcat aattctacc 
      gtcacaaga agtggttga tagttgata aacaatttt 
      ttgcgtttt gcaaattac agaaaataa gtgacgatt 
      agagtatga tgaatgtgt gccgagtgt gtttaagtg 
      acaaagact tgaaagagt tgttcgccg tgagccccc 
      ataatggtg cagtaaatt tcctggccc tggtgcaga 
      tacataaaa acgacggtc ttccccaac tcatctgtc 
      ctcaatgtc tcgatgaac tcgccactt gcgttcgtt 
      ttttcgata aatgcttat caactcatg caaagctca 
      aaaaccaca agtgcctca agtgtggcc aaatatatc 
      cttcgcata aatcgctaa caatccaca taagccgcc 
      aacacactc ctacagtta tcgtgcaaa aaaatcaca 
      agtcgggcc aacagtgtc acatttgtg ggaaaattt 
      tcacgtaca gggttgaac tgagccggc acttgtgct 
      cccacgtgg gcttacggc aatacaagt gcgataagt 
      gtggcaaaa gctacataa ctaaggcct cccttaata 
      agcacatcg taaaacatg aggggctga ggagatttg 
      agtgtctcc tttgtagta aaagtttcc tccatcagg 
      acaacttac aaacgcatt taaaaacac acagtgaga 
      aaaaatacg aatgcaggg tgtgcacaa aaaaattca 
      agtcaaatc gttcacttc tcatgcact tgaaaatcc 
      atgctaacg agagggatt ttccgtgct ccgagtgcg 
      agaaagcgt tcgtcaaca aatacgact tgttacgac 
      atttgaaac tgcacgaga aaaaaagac tgatcgcaa 
      gtaacacgt acgataaga ctaaagacg atttactca 
      aaacgaaaa atatgagac agtgcgacc aatgcggga 
      aaatctgtt cgagtcatg gcgatttga ttaaacaca 
      tgagggtgc actcggagg aacgaccct tttgttgta 
      aaaattgca acaaagggt ttaagtcag ccagtaact 
      tgcgatgcc acgaaagga tacatacaa aagtaggac 
      aaaaagcac tttggagaa ataatactt tatttgtta 
      cagagtaag ccatacatt tgtaaattc tgctcgaag 
      gcgttttgc cagtcgtcc aacttgcgc acccacgaa 
      aaaatccac tcggggaca cgccctcac atttgcaaa 
      ctgtgccct aaaaaattc gtccgtaaa agagacttg 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY