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Gene information
General information
TC013355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10605722..10617807
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013355
    iBeetle screen iB_05421

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    legs pretarsus fused - Number of animals showing the phenotype: 4


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013355 RA
      MAVIQRTDC GGMSIRLLF TQGILVLVL TGVWGDPVR 
      SCEQAKNDI EAAKLPKSW LQESPAGGD ICGPSSCCS 
      IKSELEMRK TSESLHHFT MSQIFGLSS VVDTRARKF 
      DGLFRSLMN ASKLQFHDM FQRTYGKIY LQNSDVFSN 
      FFDKLETYY NRGASRLSD TLDAFFVIL YQRMFTVIN 
      NQYQFDEKY LDCISDHMA DVKPFGDAP RKFTLELKR 
      SFVATRAYF MALATAASA AKKLQSVAI DRECERDLV 
      NMLYCGSCN GMRGLGPCS DYCTAVVGS CLRHHTAFS 
      SDWDNFIDA LDKVADRLL GPYNIELVV EPLNVKISE 
      AIMNFQESG TEVSQKIFK MCGQPTLDR KRREANYKD 
      SLEDNPNYE IKRYPPLKR IHGSKKKKT KFSYMNGNE 
      TPLEKLVRE IKLKLADTR QFWAHLPYQ YCNSISAPL 
      SSGQCWNGV SVGEYTGNV TRPPLLLST VVKDQIYHL 
      QVYTDTLRK AYHGEEVEL VDDSDDLLD GSGSGSGDF 
      EIAAPDYAP KAEDIVPKV AEEAPPPST AGPEVTRPY 
      SGTAKMSLS KALAQFLLP IVLVWFGGA ISDLL
    CDS sequence
    • >TC013355 RA
      atggctgtg attcaaagg actgactgt ggggggatg 
      agtatacga cttttattt acacagggt attttggtt 
      ttggtgcta acgggggtg tggggcgac cccgttcgg 
      tcctgcgaa caggccaag aacgacatt gaagcggcc 
      aaacttccc aaaagttgg ctccaggaa tcgccggcc 
      ggtggagac atctgcggg cccagctcc tgttgctcg 
      ataaaatcc gagctggag atgcggaaa acgagcgaa 
      tccttgcac cacttcacg atgagccag atcttcggt 
      ttgtcgtcg gtcgtcgac acacgggcc cggaaattc 
      gacggactt ttccgctcc ctgatgaac gcctccaag 
      ctgcagttc cacgacatg ttccaacgg acgtacggc 
      aagatctac ttgcaaaac tcggacgtt tttagcaat 
      tttttcgac aaactggag acgtactat aaccggggc 
      gcgagccgc ctctccgac accctggac gccttcttc 
      gtaattctc tatcagagg atgttcacc gtcatcaac 
      aaccaatac cagttcgac gagaaatat ttggactgc 
      atttccgat cacatggcc gacgtcaag cccttcggg 
      gacgccccc cggaagttc accctggag ctgaaaagg 
      tcgttcgtg gccacgagg gcctacttc atggccctt 
      gcgaccgcc gcctccgct gcgaagaag cttcaaagt 
      gtggcgatt gacagggag tgcgagagg gacttggtg 
      aatatgctt tattgcggc agttgtaat gggatgagg 
      ggcttgggg ccctgctcg gactattgt acggccgtc 
      gtggggtcg tgtttgagg catcacacg gcttttagc 
      tccgattgg gataatttt atagacgca ctcgataag 
      gtcgctgat cggttactc ggaccttac aacattgaa 
      ctagtcgtc gaaccgctc aacgtaaaa atctccgaa 
      gcaataatg aactttcaa gaatccggg accgaagtc 
      agtcagaaa attttcaag atgtgtggt cagccgacc 
      ctcgataga aaacgaaga gaagcgaat tataaagac 
      agtttggag gacaatccc aactatgag attaaaaga 
      taccccccg ttgaagcgc atacacggc agtaagaaa 
      aagaaaacc aagttttcc tacatgaat gggaacgaa 
      actccgctc gagaaactc gtcagggaa atcaaattg 
      aaattggcc gacacgcgc cagttttgg gcccacctg 
      ccctaccag tactgcaac agcatctcg gcccccctc 
      agttcgggg cagtgctgg aacggcgtc tccgtcggc 
      gaatacacc gggaacgtg acgcggccg ccgctgctg 
      ctctccact gtggtgaag gaccagatt taccacctg 
      caggtgtac accgacacg ctccggaag gcgtaccac 
      ggcgaggag gtggagctt gtggacgac tcggacgat 
      ctcctggac gggagcggc tccgggtcg ggcgatttc 
      gagatcgcc gcccccgac tacgcgccc aaggcggag 
      gacatcgtc ccgaaagtg gcggaggag gcgccgccc 
      ccgagtacg gcggggccg gaagtgaca cggccgtac 
      tcggggacg gcaaagatg tcgctgtcc aaagccctg 
      gcgcagttt ctactgccg atagtgctt gtctggttc 
      ggaggtgcc atatcggac ttactgtga
    mRNA sequence
    • >TC013355 RA
      tgcacattc ttgcggtcc gaacagtcg ccggagtca 
      ccggaatgt gaatgtcgg gggttgagt gccttagca 
      accggagct cgtgatggg gcgaagcta gactttggg 
      aaaaaatcg ctgtgtgat taattaatt aaaaaaaaa 
      ttgaagcga tggctgtga ttcaaagga ctgactgtg 
      gggggatga gtatacgac ttttattta cacagggta 
      ttttggttt tggtgctaa cgggggtgt ggggcgacc 
      ccgttcggt cctgcgaac aggccaaga acgacattg 
      aagcggcca aacttccca aaagttggc tccaggaat 
      cgccggccg gtggagaca tctgcgggc ccagctcct 
      gttgctcga taaaatccg agctggaga tgcggaaaa 
      cgagcgaat ccttgcacc acttcacga tgagccaga 
      tcttcggtt tgtcgtcgg tcgtcgaca cacgggccc 
      ggaaattcg acggacttt tccgctccc tgatgaacg 
      cctccaagc tgcagttcc acgacatgt tccaacgga 
      cgtacggca agatctact tgcaaaact cggacgttt 
      ttagcaatt ttttcgaca aactggaga cgtactata 
      accggggcg cgagccgcc tctccgaca ccctggacg 
      ccttcttcg taattctct atcagagga tgttcaccg 
      tcatcaaca accaatacc agttcgacg agaaatatt 
      tggactgca tttccgatc acatggccg acgtcaagc 
      ccttcgggg acgcccccc ggaagttca ccctggagc 
      tgaaaaggt cgttcgtgg ccacgaggg cctacttca 
      tggcccttg cgaccgccg cctccgctg cgaagaagc 
      ttcaaagtg tggcgattg acagggagt gcgagaggg 
      acttggtga atatgcttt attgcggca gttgtaatg 
      ggatgaggg gcttggggc cctgctcgg actattgta 
      cggccgtcg tggggtcgt gtttgaggc atcacacgg 
      cttttagct ccgattggg ataatttta tagacgcac 
      tcgataagg tcgctgatc ggttactcg gaccttaca 
      acattgaac tagtcgtcg aaccgctca acgtaaaaa 
      tctccgaag caataatga actttcaag aatccggga 
      ccgaagtca gtcagaaaa ttttcaaga tgtgtggtc 
      agccgaccc tcgatagaa aacgaagag aagcgaatt 
      ataaagaca gtttggagg acaatccca actatgaga 
      ttaaaagat accccccgt tgaagcgca tacacggca 
      gtaagaaaa agaaaacca agttttcct acatgaatg 
      ggaacgaaa ctccgctcg agaaactcg tcagggaaa 
      tcaaattga aattggccg acacgcgcc agttttggg 
      cccacctgc cctaccagt actgcaaca gcatctcgg 
      cccccctca gttcggggc agtgctgga acggcgtct 
      ccgtcggcg aatacaccg ggaacgtga cgcggccgc 
      cgctgctgc tctccactg tggtgaagg accagattt 
      accacctgc aggtgtaca ccgacacgc tccggaagg 
      cgtaccacg gcgaggagg tggagcttg tggacgact 
      cggacgatc tcctggacg ggagcggct ccgggtcgg 
      gcgatttcg agatcgccg cccccgact acgcgccca 
      aggcggagg acatcgtcc cgaaagtgg cggaggagg 
      cgccgcccc cgagtacgg cggggccgg aagtgacac 
      ggccgtact cggggacgg caaagatgt cgctgtcca 
      aagccctgg cgcagtttc tactgccga tagtgcttg 
      tctggttcg gaggtgcca tatcggact tactgtgat 
      aagttattg taaataaag tgaagtcat tagggaccc 
      gagactttt tgtgtacat gttttgtaa catttccca 
      tgtaatgtg aactttaga tgtatttat attaacaaa 
      gaaaaatat acaaaatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY