Status
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Gene information
General information
TC013339 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10918747..10921664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013339
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05420

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_10086

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013339 RA
      MFAFFTLFL TAATVTYCD NFSSINLPD SHLPYYFTN 
      FPKIREKCD KDPQCPYKT SLNSPKCWG YEYNCLESE 
      RFSAPRCPG DHKGWVKSK SDQINTFYT QADFGFVKQ 
      QLREMKLLC EPLFADDSS LECSEHLRF CRGRNLMIN 
      FTDLRTRED PIRYKMDVL KDGQIGGYC DFKQAALEE 
      QTDHLSPLQ SWAPEMRYF ARLARRPIV EGDCDVVIE 
      KPTFIMKID ATVNMYHHF CDFLNLYAS IHLNSTQWD 
      AFSTDVHVL IWETYTYRS AFGDTWEAF TDHPVWDLK 
      TFRGETVCF KNVVFPLLP RMIFGLYYN TPIIYGCEN 
      SGLFQAFSQ HILHRLKIP FHPRNNRKI RITLLARDT 
      KYRRILNED ELVEALAKN HDYEVQKVV YNKDVPFKK 
      QLEITRNSD VLIGVHGAG LTHLLFLPD WAAVFELYN 
      CEDANCYFD LARLRGLKY VTWEKLDKL KSQEDGTHD 
      GGAHAKFAN YSFDVKEFL RLVAKAAKH VENHPDFKN 
      LISKLDNEM DHDEL
    CDS sequence
    • >TC013339 RA
      atgttcgca ttttttacg ttattttta accgcggcc 
      accgtaacg tattgtgat aacttttcc agtattaac 
      ctccccgat tctcactta ccatattac ttcactaac 
      tttcccaaa atcagggaa aagtgcgac aaagaccca 
      caatgccct tacaaaacc tctctaaac agccccaaa 
      tgctggggc tacgaatac aactgtctg gaatcggag 
      cgcttttca gcgccccga tgcccaggc gaccacaaa 
      ggctgggtc aagtccaag tccgaccaa attaacact 
      ttttacacc caagccgat ttcggcttt gttaagcaa 
      caattgcgc gagatgaaa ctgctctgt gagcccctt 
      ttcgccgac gatagttct ctcgagtgt tcggaacat 
      ttgcgcttt tgtcggggg cgcaacctc atgatcaac 
      ttcaccgat ttgcggacg cgcgaggac ccaatcagg 
      tacaaaatg gacgtttta aaagacggc caaattggg 
      ggctactgc gactttaaa caagcggca ctggaggag 
      cagacggac cacttgagc cccttgcag agctgggcc 
      cccgaaatg aggtacttc gctaggctt gcgcgtagg 
      ccgatagtc gaaggcgat tgcgatgta gtgatagag 
      aaacccacg tttattatg aaaattgac gctacggtg 
      aatatgtac caccatttc tgcgatttt ctcaatctt 
      tatgcttca atacatttg aattcgacc caatgggac 
      gctttctcg accgatgtt cacgtgttg atttgggag 
      acttacacg tacaggtcg gcgttcggg gacacctgg 
      gaggctttc accgatcat cccgtttgg gatttgaag 
      acttttagg ggcgaaacc gtgtgtttt aagaacgtg 
      gtgttcccc cttttgcct cgcatgatt ttcgggctg 
      tattacaac accccgatt atctacggg tgtgagaac 
      agcggcctg tttcaagcc ttctcccaa catatttta 
      caccgactc aaaatcccc tttcaccct cggaacaat 
      aggaaaatt agaattacg cttcttgcc cgcgatacg 
      aaatatcgc aggattttg aacgaggac gaacttgtt 
      gaagccttg gcgaaaaat catgactat gaagtgcaa 
      aaagttgtc tacaataag gacgttccg ttcaagaaa 
      cagttagag attacgcgg aattccgat gttttaatt 
      ggggtacat ggggctggc ttgacgcat ttattgttt 
      ttaccggac tgggcggct gtttttgaa ttgtacaac 
      tgtgaagat gcgaactgt tatttcgat ctggcaagg 
      ttaaggggg ctcaagtat gtcacgtgg gagaaactg 
      gataaacta aagtcacag gaggatgga actcatgat 
      ggtggggct cacgccaaa ttcgccaat tactctttc 
      gacgtaaaa gagttttta cgattagtt gcaaaggca 
      gcaaaacac gttgaaaac catcccgat tttaaaaat 
      ttaatttcc aaacttgat aatgaaatg gaccatgac 
      gaactgtaa
    mRNA sequence
    • >TC013339 RA
      tttttttgt gtccattat ttacatatt tgtacttta 
      aaataatgt cttcagaca gttccgaag acgaagact 
      tggataaat tacgcgaag ccgccgaca gccagctgt 
      taaataacg ccttataca acaaaaccg aaccgaaat 
      tggaggaac ccccgaaca cgaaaccac agtcattac 
      gccgtagca aggacgagg acgagcaat ttaatctgt 
      tccgtgtat ccccagaat tccaaaatt acgtcgcca 
      agcatttgt ctcaaatcc tcgataaat tgttaaaag 
      aaagactcg aaacgaaaa actacgaaa attacgtga 
      aaccaaaac gacggaaaa gtgggatca aattacttc 
      gtgattcag tgcattgca taagtgtaa aaacaagag 
      acgttgcgc cacaggtga aaaaaaggc aagttgagg 
      aggacgaaa gcgacaacg aggctaaat ttcgagctg 
      tggccgtaa gtgctgacg atattttaa gcaaaaaag 
      acacaaaac actggtcta cacgtagca aagcgcccg 
      tttatgcct attacaaaa cgcagcaag gccttatcc 
      ttaaataaa taaagtttc gttcatttt ttaatgtat 
      tttaccgtt caattccac ttttttgtt gaaataaat 
      tcaaaaaaa aatttttct tggttggta gcgctgacc 
      attaacctc aaccttcga aagctaacc tcaaaaccg 
      tgccaatga gttttcctc gcctaattc gcttatttt 
      agtgttaaa aactccaca atgttcgca ttttttacg 
      ttattttta accgcggcc accgtaacg tattgtgat 
      aacttttcc agtattaac ctccccgat tctcactta 
      ccatattac ttcactaac tttcccaaa atcagggaa 
      aagtgcgac aaagaccca caatgccct tacaaaacc 
      tctctaaac agccccaaa tgctggggc tacgaatac 
      aactgtctg gaatcggag cgcttttca gcgccccga 
      tgcccaggc gaccacaaa ggctgggtc aagtccaag 
      tccgaccaa attaacact ttttacacc caagccgat 
      ttcggcttt gttaagcaa caattgcgc gagatgaaa 
      ctgctctgt gagcccctt ttcgccgac gatagttct 
      ctcgagtgt tcggaacat ttgcgcttt tgtcggggg 
      cgcaacctc atgatcaac ttcaccgat ttgcggacg 
      cgcgaggac ccaatcagg tacaaaatg gacgtttta 
      aaagacggc caaattggg ggctactgc gactttaaa 
      caagcggca ctggaggag cagacggac cacttgagc 
      cccttgcag agctgggcc cccgaaatg aggtacttc 
      gctaggctt gcgcgtagg ccgatagtc gaaggcgat 
      tgcgatgta gtgatagag aaacccacg tttattatg 
      aaaattgac gctacggtg aatatgtac caccatttc 
      tgcgatttt ctcaatctt tatgcttca atacatttg 
      aattcgacc caatgggac gctttctcg accgatgtt 
      cacgtgttg atttgggag acttacacg tacaggtcg 
      gcgttcggg gacacctgg gaggctttc accgatcat 
      cccgtttgg gatttgaag acttttagg ggcgaaacc 
      gtgtgtttt aagaacgtg gtgttcccc cttttgcct 
      cgcatgatt ttcgggctg tattacaac accccgatt 
      atctacggg tgtgagaac agcggcctg tttcaagcc 
      ttctcccaa catatttta caccgactc aaaatcccc 
      tttcaccct cggaacaat aggaaaatt agaattacg 
      cttcttgcc cgcgatacg aaatatcgc aggattttg 
      aacgaggac gaacttgtt gaagccttg gcgaaaaat 
      catgactat gaagtgcaa aaagttgtc tacaataag 
      gacgttccg ttcaagaaa cagttagag attacgcgg 
      aattccgat gttttaatt ggggtacat ggggctggc 
      ttgacgcat ttattgttt ttaccggac tgggcggct 
      gtttttgaa ttgtacaac tgtgaagat gcgaactgt 
      tatttcgat ctggcaagg ttaaggggg ctcaagtat 
      gtcacgtgg gagaaactg gataaacta aagtcacag 
      gaggatgga actcatgat ggtggggct cacgccaaa 
      ttcgccaat tactctttc gacgtaaaa gagttttta 
      cgattagtt gcaaaggca gcaaaacac gttgaaaac 
      catcccgat tttaaaaat ttaatttcc aaacttgat 
      aatgaaatg gaccatgac gaactgtaa tttattaca 
      aactttgac taattgtta ctaatgaga ttaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY