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Gene information
General information
TC013280 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6045731..6047370
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013280
    iBeetle screen iB_05414

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    mandible not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013280 RA
      MFLKYFAVL LCALHINIL NAGIIPNPY IVRSNLLPD 
PTACGVFVE NKIFGGKKT ELDEFPWMV LLEYHRCGK 
REFDCGGFL INNRYVVTA AHCIDDELK SVRLGEWNL 
DTNPDCSAV DNCAPPVVD IPIEEKITY KENSSGVSS 
RHDIALLRL KHEVQYSDF IKPICLPNT VDEITKSYV 
DQKLIVTGW GFTEANKSS NIKLKVKVP VKKSSDCEV 
GFRNAYNVD ISLSEYEMC AGGEKGKDS CVGDSGGPL 
MTLRRDKNK DPRYVAVGV VSSGPAKCG SENQPGVYV 
RVVKYVSWI ISNLKI
    CDS sequence
    • >TC013280 RA
      atgttccta aagtacttc gcagtttta ctgtgtgca 
ttgcacata aatattctc aatgcgggc attattcca 
aacccttac attgtcaga agtaactta ttgccagat 
cccacagct tgtggagtt tttgtggaa aataaaatt 
tttggtgga aagaaaacc gagttggat gagtttccc 
tggatggta ttgctcgag taccacagg tgtggtaaa 
agggaattt gattgtgga gggtttctt atcaataat 
agatacgta gttacagcc gcacattgt attgatgat 
gagttaaaa agtgttcgt ttgggcgag tggaatctg 
gatactaat cctgactgt tctgccgtt gacaattgt 
gcaccacct gttgttgat attcccata gaagagaaa 
atcacttat aaggagaat agttcaggt gtaagctct 
cgccacgac attgctctt ttacgatta aagcacgaa 
gttcaatac tcagacttt attaaacca atttgtctt 
ccaaacacc gttgacgaa attacgaag tcttacgtt 
gaccaaaag cttatcgtg actggttgg ggctttact 
gaggcaaat aaaagtagt aacatcaaa cttaaagtc 
aaagttccc gttaaaaaa tcgtctgat tgtgaagtt 
ggatttaga aatgcttat aacgtcgac ataagtttg 
agtgaatac gagatgtgt gcaggagga gaaaaaggc 
aaagattcg tgtgtgggt gatagtgga gggcctttg 
atgactctc agaagagac aaaaacaag gacccgaga 
tatgttgct gtgggggtg gtgtctagt ggaccagct 
aagtgtggg tcggaaaat caaccaggg gtttatgtg 
agagttgtt aaatacgtc tcttggatt attagtaat 
ttaaaaatc taa
    mRNA sequence
    • >TC013280 RA
      atcctgtta tttgattta ataacataa tatattaca 
caattagta gtaaagcag gtatgtagg tacttccca 
aacacgttt tttaaaatt gtggaatca caaagaatg 
acattgtaa actcaatga acagtaaca caggtcagt 
aaccataaa cagtaaata cttcgcata cgcgctgtg 
ttatccttg actgatctg cagaagtgt gtggcttgt 
ttatgcgcc agtaattca agaagtgga ttttcctgt 
ctttcatat actataaaa tcaagatcg tggaagcac 
atttcctag cgcgcagct gagcaaact gttgtaaat 
aatttgttt ataatgttc ctaaagtac ttcgcagtt 
ttactgtgt gcattgcac ataaatatt ctcaatgcg 
ggcattatt ccaaaccct tacattgtc agaagtaac 
ttattgcca gatcccaca gcttgtgga gtttttgtg 
gaaaataaa atttttggt ggaaagaaa accgagttg 
gatgagttt ccctggatg gtattgctc gagtaccac 
aggtgtggt aaaagggaa tttgattgt ggagggttt 
cttatcaat aatagatac gtagttaca gccgcacat 
tgtattgat gatgagtta aaaagtgtt cgtttgggc 
gagtggaat ctggatact aatcctgac tgttctgcc 
gttgacaat tgtgcacca cctgttgtt gatattccc 
atagaagag aaaatcact tataaggag aatagttca 
ggtgtaagc tctcgccac gacattgct cttttacga 
ttaaagcac gaagttcaa tactcagac tttattaaa 
ccaatttgt cttccaaac accgttgac gaaattacg 
aagtcttac gttgaccaa aagcttatc gtgactggt 
tggggcttt actgaggca aataaaagt agtaacatc 
aaacttaaa gtcaaagtt cccgttaaa aaatcgtct 
gattgtgaa gttggattt agaaatgct tataacgtc 
gacataagt ttgagtgaa tacgagatg tgtgcagga 
ggagaaaaa ggcaaagat tcgtgtgtg ggtgatagt 
ggagggcct ttgatgact ctcagaaga gacaaaaac 
aaggacccg agatatgtt gctgtgggg gtggtgtct 
agtggacca gctaagtgt gggtcggaa aatcaacca 
ggggtttat gtgagagtt gttaaatac gtctcttgg 
attattagt aatttaaaa atctaaaac taagtcatg 
attgtgaaa atactaaaa ccattgaaa atttgcagt 
gttaattta tcaacttct catacactt ttttgagag 
actgtaata tacactaat aggttaagt tataataag 
ttatttatg tgttgcaaa ttgtaaata ccaaaacaa 
gactaacat atgtaataa atctgtatt caataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY