Status
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Gene information
General information
TC031151
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013268,TC013269,TC013267,TC013270
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11320583..11340985
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031151
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05408

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      beetle stock: bad (increased lethality),
      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature potentially partiallythinner - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      larva appendages shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
      larva appendages swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

      antenna shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
      antenna not present - Penetrance (percentage of animals/eggs on slide): more than 80%
      legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      iBeetle screen iB_05409

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031151 RA
        MMEGLNQLG QEEYIWAMR TSGRRDSDV EVRKAGRSY 
        QSGLLGKKS KSVVESTDD RQTKVMASL VPDEVIYNV 
        DDYTHRKYD ACFLFGDVS GFTELCEKY TKTGVAGPS 
        RMTQVLNKY LGSMVQEVM SHNGDVLKF SGDAFLAIF 
        KTSDTETMR DAVHEALDC ALVIQKNYG SYMTDVGVA 
        IRAISAGLV TFALIGDST NSHYVVVGQ PIWDVKAAE 
        SISSAGDIV VAPAAWHFV NPNEYLSEE MSDNIHVKI 
        VGVGPNWRS VQKNLRQQK PDDETGEDV VSQDEESTE 
        SDVLSEITQ DGALDEFSL RPAVNLAVR LHIKEALRR 
        FIIAPVMKS IDMDEPFEY LTEMRQAVI VFINVITYK 
        LETEKTVIL ADQTYKTVC RVVDKMKGC VNKVSLFDK 
        DLMFVVIFG LRGLKHELE CQVALRCAR QCHTAIDKL 
        PGVMSTSIG VTTGKTYCG VFGHTLRRE YTVISLIVN 
        KAARLMVAY EGKVTCDRE TFLHSKLES RHFILQPHK 
        YLKGISHPG PVYEFREVE IDHDHVNKV NACPLLGRE 
        RNIKLYKQL LDKCLHSTD SNEPQYKML LISGDARQG 
        KTRLLDELV YLTPSEIPV SKVVLTELD QNIPYQCVK 
        LIFGPPLLI SKSSTSHKK QQVIQDKLN KIQVPELMC 
        VLNNVFDVN FKVSDLYMS LKVQARKIA LVKMIKQLC 
        YACFTTVWV VAIDNLDFM DAESSSLFD CFFDIKTIF 
        IAATRGRER LLQEVMSQK IVEDPRVKH VQLLPIERW 
        YLGALACQI LGVYAMSPE LENAIQVRS NGNPGWVES 
        FLISLIQDE GLYILKATL KDINDLGLV CPPLYMMAR 
        LTKDQMRKW QHIMEERRP SIDSISAEE DNWKRYIDS 
        CRDSHLNVT VREEMEAIS RGGKVPICI ISPTFHVAD 
        EEMELSMDA MILKTFDSL NFYEQLLVK CSATLGNQF 
        LRDMLLYVM SATEKRKAA LAVQKLFEI RVLSCAKGD 
        FMEGGTKIF KEKLLDPDD EMRLKCDCR GIKIEEECK 
        DLPKYASCG YLQFRSSLF RDITYNLLT DNQKKEFHQ 
        RAIRFLERE TRKCQACGG GFFHRILGA HHDKEFIRA 
        RDKQKKTFS GVTSWEGVS HRTSMHSMS IRSESHFSA 
        HSQSYQSFH SKRSRFDSV AGGSMGSMP SQIFGEIKE 
        DKSYDAFFG FSIINKLKN SFSLTKAFS SEDFTDCQC 
        NLILSTTYA QLIEHCKGA GDLGKEIEA LLEYAQICI 
        IASNLPLAM SILDESLDV LREKAKERA ENIWKVALI 
        RAKIYCLIG YSRLELGHL DEALLSLNL SLNEYGITF 
        PKGINKHLK LYAHKVKQL FGLYLFPKS LTKNLDHWE 
        TIFANNLAE CLSHFCTLF MIKKEWKNA EMAAVWGLT 
        KSLESFSDF RVICSACAN MIHVAQYFH NWSLCVALE 
        VHALRLCHL KRASIEAED IKCVCKLYC VIFVSRVFM 
        ANLDKAIHL GYIVLRLCS SVHAVRVIL PLLPTLIQC 
        LIMGKHFAN AYSVLQELK LFAEDDTDN SGKIWYYAI 
        CVGLQLETG YTLVPYHIC EHFYQLEGE NWVNIRDSD 
        ARRRFFTVM WLWCIRNEK WEAAYIWCY KTSDFMSVS 
        EKDSLANKI TSLYVLEGL LLYFVNKLD KRNIKAIIR 
        VEHEINSLF KTIEKISKT VKVLIPRLY HLKAYYKYI 
        KMSDSNAID MLTKASNYA HKYGNSLEE GWIDHSVKA 
        WNKTLQANA IEFWKEHSE AENVLDFQD IEITGETLG 
        YYTLPTPLY E
      CDS sequence
      • >TC031151 RA
        atgatggaa ggattgaat cagttgggg caggaggag 
        tacatctgg gcaatgagg acgagcggt cgtcgagac 
        tcagatgta gaagtgcgt aaagcaggt cgttcctac 
        caatcgggc ttattgggg aagaaatca aaaagtgta 
        gtggagtcc accgacgat cgccaaact aaagtgatg 
        gcatctctc gtccccgac gaagtgatt tacaatgtt 
        gacgattat acacaccgg aagtacgac gcttgtttc 
        ctctttggg gacgtttcc ggtttcacc gaactgtgc 
        gaaaagtac accaagacg ggtgttgct ggtccgtcg 
        agaatgacc caagtgttg aacaagtac ttgggttca 
        atggtccaa gaagtcatg tcgcacaat ggtgatgtc 
        ttgaagttt tctggagac gcgttcctt gcgattttt 
        aagacatca gacaccgaa acaatgcga gacgcagtc 
        cacgaagcc ctggactgt gccctagtc attcaaaaa 
        aattacggt tcttacatg accgacgtc ggtgttgca 
        attcgagca atttctgcc ggtttggtg acttttgcc 
        ttgattggt gattcgacc aattcccat tacgtggtg 
        gttggtcaa cccatatgg gacgttaag gccgccgaa 
        agtattagc agtgctggt gatattgtt gttgcacca 
        gcggcctgg cattttgtc aaccccaac gagtatctc 
        tcggaagag atgagtgac aacatccac gtgaaaatc 
        gtaggagtt ggcccgaat tggcgaagc gttcagaaa 
        aatctccgt caacagaag ccagacgac gaaaccggc 
        gaggatgtg gtaagtcaa gatgaagaa tcgacagaa 
        agtgatgtt ttgagtgaa ataacgcaa gatggggcc 
        ttggacgaa ttctctttg agaccggca gtaaaccta 
        gcagtcaga ctccatatc aaggaagct ttaaggcga 
        tttataatc gcccccgta atgaaaagt attgacatg 
        gacgaacct ttcgaatat ctcaccgaa atgagacaa 
        gctgtgatt gtgttcatt aacgtcatt acgtacaaa 
        ctagaaact gaaaaaact gtcattttg gccgaccaa 
        acgtacaaa accgtctgc agagtggtc gacaagatg 
        aaaggctgt gtgaacaaa gtctcactc ttcgataaa 
        gatttgatg tttgttgtt atattcggc ctacgtggc 
        cttaaacac gaactagag tgtcaagtc gcgctaaga 
        tgtgctaga caatgccat accgctatc gacaaactt 
        cctggtgtt atgtcaacg tcgatcggt gttactaca 
        ggtaaaact tattgtggc gtctttgga cacacgctc 
        cgtcgtgaa tatacagtt atcagtctt attgtcaac 
        aaagcagct cgcttgatg gttgcttac gaagggaaa 
        gtaacttgt gatagggaa acgtttctg cacagtaaa 
        ctagaatct agacatttt atcttgcaa ccacacaaa 
        tacttgaag ggaatatct catccaggc ccggtttac 
        gagttcagg gaggtcgaa atagaccat gatcatgtt 
        aataaggtc aatgcgtgt cccttgctg ggacgagaa 
        cgcaatata aagttatat aaacaattg ttggataaa 
        tgcttacat tctaccgat tctaatgag ccacagtac 
        aaaatgttg ctgatttcg ggcgatgct cggcaagga 
        aaaacgcga cttctcgat gagctcgtt tacctaaca 
        ccgtcagaa ataccagtt agtaaagta gtactgacg 
        gaattagac caaaatatc ccgtatcaa tgtgtgaag 
        ctgatattt ggccctcct ttgctaata tctaaaagc 
        tcaacctcg cataagaaa cagcaggta attcaagac 
        aagttgaat aagatacaa gtccccgaa ctgatgtgc 
        gttttgaac aatgttttc gatgtcaat ttcaaagtt 
        tcggatctt tatatgtcg ctgaaggtg caagcgcga 
        aaaatagca ctagtcaaa atgatcaag cagttatgt 
        tacgcttgt tttacaacc gtttgggtg gttgcgatc 
        gacaatctt gactttatg gacgcggaa tcgtcctca 
        ctttttgat tgttttttt gatattaag accatattc 
        attgcggca actagaggg cgtgagagg ttgctccaa 
        gaagtcatg tcacaaaaa attgttgaa gatcccaga 
        gtcaaacac gtccaactc cttcccata gaaaggtgg 
        tatttaggg gcacttgcg tgccaaatt ttgggtgtc 
        tatgccatg tcaccagaa ttggaaaac gccatacag 
        gtcaggagt aacggcaat cctggttgg gttgaaagt 
        tttttaatt agtttgata caagatgaa ggattgtat 
        attttaaaa gcaactctt aaagatatt aacgatctt 
        ggacttgtt tgcccacct ctgtacatg atggccaga 
        ttgactaaa gatcaaatg cgaaaatgg caacacatt 
        atggaagaa aggcgacca tctatagat tcaatcagt 
        gcagaggag gacaattgg aaacgatac atcgacagc 
        tgtcgagac agtcacctc aacgtcact gttagggag 
        gaaatggaa gccatttca agaggcggg aaagtcccc 
        atatgcata atttcccca acttttcac gttgcggac 
        gaagaaatg gagctttct atggacgcg atgatccta 
        aaaacgttc gattcgctc aatttttac gagcaattg 
        ctagtcaaa tgtagcgct actttgggg aatcagttc 
        cttcgggat atgcttttg tacgtaatg tcagcgacg 
        gaaaaacga aaagcagct ttagccgtt cagaaactt 
        tttgaaatt agggtgctt tcatgcgct aagggcgat 
        tttatggaa ggggggacg aaaattttc aaggagaaa 
        ttgttggat cccgacgat gaaatgcgc ttgaaatgt 
        gactgtaga gggattaaa attgaagag gaatgtaaa 
        gatctgccc aaatatgct tcgtgtggt tacttacag 
        ttccggtca tcgctattt cgtgatata acatacaat 
        ctgttaact gataaccaa aagaaagag tttcatcaa 
        cgtgccatc aggtttctc gaacgtgaa acacgaaag 
        tgtcaagct tgtggtggt ggttttttc catcgaatt 
        ttgggcgcc caccatgac aaggagttt atacgagcc 
        agagacaag caaaagaaa actttttcc ggagtcacc 
        tcatgggag ggcgtaagt catcgaaca tcaatgcac 
        agcatgtcc atacgttcc gagagccac ttcagtgcg 
        cacagccaa agttaccaa agttttcat agtaaaaga 
        tccagattc gattcggtg gcgggagga tcgatgggg 
        tcaatgcct agccaaatt tttggcgaa atcaaggag 
        gacaaatcc tacgatgca tttttcggc ttttcgata 
        atcaacaaa ttgaaaaac agtttttcc ttgacaaaa 
        gccttctcc tcggaggat tttacagac tgccagtgt 
        aaccttatt ttgagcacg acttatgcc cagttgatt 
        gagcactgc aagggggcg ggagatttg ggcaaggag 
        attgaggcg ttgctggaa tacgcgcaa atttgcata 
        atcgcgtca aatctcccg cttgcgatg tccattctg 
        gacgaatcc ttggacgta ctgcgagag aaagcgaag 
        gaacgagct gaaaacata tggaaggtg gcactaatc 
        cgtgctaag atctactgt ctgattggt tactcgaga 
        ctagagctg gggcacttg gacgaggcc ttgctgagt 
        ctaaattta tcgttgaac gaatacggg atcacgttt 
        ccaaaaggg attaacaag cacttgaag ctgtatgcg 
        cataaagtc aagcagttg tttggttta tatctcttt 
        cccaaatct ttgaccaag aacttggat cactgggaa 
        accatcttt gctaataac cttgccgaa tgtttgtcg 
        catttctgc acgctattt atgattaaa aaagagtgg 
        aagaatgcc gaaatggct gcagtttgg ggcttgacc 
        aaatctttg gaatctttc agtgacttt cgcgtaatt 
        tgttcagct tgtgcaaat atgatacat gttgctcaa 
        tattttcac aattggagt ttatgtgtt gctttagaa 
        gtgcacgct ttgagactg tgccatttg aaaagagct 
        tcaatcgaa gccgaggat atcaagtgc gtgtgcaag 
        ctgtattgc gtgattttt gtttcaagg gttttcatg 
        gcgaatttg gacaaagcc atccacttg ggctacatc 
        gttctgaga ttatgttca tcagtgcat gccgttcga 
        gtgattctt cccttactc ccgaccctg attcagtgc 
        cttataatg gggaagcac tttgctaat gcgtattct 
        gtccttcaa gagctcaaa ttatttgca gaggacgat 
        acggataac tcaggaaaa atatggtac tacgcgatt 
        tgtgttggt ttacaactc gaaacaggc tacactttg 
        gtcccgtat cacatatgt gaacatttc tatcaactt 
        gaaggcgaa aactgggtt aatattaga gattcggat 
        gcgagaaga cgattcttt accgtaatg tggttgtgg 
        tgtattaga aatgaaaag tgggaagct gcgtatatt 
        tggtgttac aagacgtct gattttatg agtgtttcc 
        gaaaaagat tctttggcg aataaaata acttcgttg 
        tatgtgttg gaagggttg ttgctgtat ttcgtaaac 
        aagttagac aaacgtaat attaaagct attatcagg 
        gtggaacac gagataaac tcgttgttc aagactatt 
        gagaagatt tccaaaaca gttaaggtt ctcataccg 
        aggttatat cacctgaaa gcttattac aagtacata 
        aagatgagt gattctaat gccattgat atgttaact 
        aaagcgagt aattatgct cataaatat ggaaactct 
        ttagaagaa gggtggatt gaccattcc gtaaaggct 
        tggaataag acacttcaa gctaatgca atagagttt 
        tggaaagaa cattcagaa gctgagaat gtactcgat 
        tttcaagac atagaaatt acgggcgaa accttggga 
        tattacaca ttaccgact ccactctat gaataa
      mRNA sequence
      • >TC031151 RA
        ctgtcattt taaaacgtt tttgtcaat ttttggcaa 
        tattttttt acgtagtcc atattttca caaaaaaat 
        cagtcaaga tgatggaag gattgaatc agttggggc 
        aggaggagt acatctggg caatgagga cgagcggtc 
        gtcgagact cagatgtag aagtgcgta aagcaggtc 
        gttcctacc aatcgggct tattgggga agaaatcaa 
        aaagtgtag tggagtcca ccgacgatc gccaaacta 
        aagtgatgg catctctcg tccccgacg aagtgattt 
        acaatgttg acgattata cacaccgga agtacgacg 
        cttgtttcc tctttgggg acgtttccg gtttcaccg 
        aactgtgcg aaaagtaca ccaagacgg gtgttgctg 
        gtccgtcga gaatgaccc aagtgttga acaagtact 
        tgggttcaa tggtccaag aagtcatgt cgcacaatg 
        gtgatgtct tgaagtttt ctggagacg cgttccttg 
        cgattttta agacatcag acaccgaaa caatgcgag 
        acgcagtcc acgaagccc tggactgtg ccctagtca 
        ttcaaaaaa attacggtt cttacatga ccgacgtcg 
        gtgttgcaa ttcgagcaa tttctgccg gtttggtga 
        cttttgcct tgattggtg attcgacca attcccatt 
        acgtggtgg ttggtcaac ccatatggg acgttaagg 
        ccgccgaaa gtattagca gtgctggtg atattgttg 
        ttgcaccag cggcctggc attttgtca accccaacg 
        agtatctct cggaagaga tgagtgaca acatccacg 
        tgaaaatcg taggagttg gcccgaatt ggcgaagcg 
        ttcagaaaa atctccgtc aacagaagc cagacgacg 
        aaaccggcg aggatgtgg taagtcaag atgaagaat 
        cgacagaaa gtgatgttt tgagtgaaa taacgcaag 
        atggggcct tggacgaat tctctttga gaccggcag 
        taaacctag cagtcagac tccatatca aggaagctt 
        taaggcgat ttataatcg cccccgtaa tgaaaagta 
        ttgacatgg acgaacctt tcgaatatc tcaccgaaa 
        tgagacaag ctgtgattg tgttcatta acgtcatta 
        cgtacaaac tagaaactg aaaaaactg tcattttgg 
        ccgaccaaa cgtacaaaa ccgtctgca gagtggtcg 
        acaagatga aaggctgtg tgaacaaag tctcactct 
        tcgataaag atttgatgt ttgttgtta tattcggcc 
        tacgtggcc ttaaacacg aactagagt gtcaagtcg 
        cgctaagat gtgctagac aatgccata ccgctatcg 
        acaaacttc ctggtgtta tgtcaacgt cgatcggtg 
        ttactacag gtaaaactt attgtggcg tctttggac 
        acacgctcc gtcgtgaat atacagtta tcagtctta 
        ttgtcaaca aagcagctc gcttgatgg ttgcttacg 
        aagggaaag taacttgtg atagggaaa cgtttctgc 
        acagtaaac tagaatcta gacatttta tcttgcaac 
        cacacaaat acttgaagg gaatatctc atccaggcc 
        cggtttacg agttcaggg aggtcgaaa tagaccatg 
        atcatgtta ataaggtca atgcgtgtc ccttgctgg 
        gacgagaac gcaatataa agttatata aacaattgt 
        tggataaat gcttacatt ctaccgatt ctaatgagc 
        cacagtaca aaatgttgc tgatttcgg gcgatgctc 
        ggcaaggaa aaacgcgac ttctcgatg agctcgttt 
        acctaacac cgtcagaaa taccagtta gtaaagtag 
        tactgacgg aattagacc aaaatatcc cgtatcaat 
        gtgtgaagc tgatatttg gccctcctt tgctaatat 
        ctaaaagct caacctcgc ataagaaac agcaggtaa 
        ttcaagaca agttgaata agatacaag tccccgaac 
        tgatgtgcg ttttgaaca atgttttcg atgtcaatt 
        tcaaagttt cggatcttt atatgtcgc tgaaggtgc 
        aagcgcgaa aaatagcac tagtcaaaa tgatcaagc 
        agttatgtt acgcttgtt ttacaaccg tttgggtgg 
        ttgcgatcg acaatcttg actttatgg acgcggaat 
        cgtcctcac tttttgatt gtttttttg atattaaga 
        ccatattca ttgcggcaa ctagagggc gtgagaggt 
        tgctccaag aagtcatgt cacaaaaaa ttgttgaag 
        atcccagag tcaaacacg tccaactcc ttcccatag 
        aaaggtggt atttagggg cacttgcgt gccaaattt 
        tgggtgtct atgccatgt caccagaat tggaaaacg 
        ccatacagg tcaggagta acggcaatc ctggttggg 
        ttgaaagtt ttttaatta gtttgatac aagatgaag 
        gattgtata ttttaaaag caactctta aagatatta 
        acgatcttg gacttgttt gcccacctc tgtacatga 
        tggccagat tgactaaag atcaaatgc gaaaatggc 
        aacacatta tggaagaaa ggcgaccat ctatagatt 
        caatcagtg cagaggagg acaattgga aacgataca 
        tcgacagct gtcgagaca gtcacctca acgtcactg 
        ttagggagg aaatggaag ccatttcaa gaggcggga 
        aagtcccca tatgcataa tttccccaa cttttcacg 
        ttgcggacg aagaaatgg agctttcta tggacgcga 
        tgatcctaa aaacgttcg attcgctca atttttacg 
        agcaattgc tagtcaaat gtagcgcta ctttgggga 
        atcagttcc ttcgggata tgcttttgt acgtaatgt 
        cagcgacgg aaaaacgaa aagcagctt tagccgttc 
        agaaacttt ttgaaatta gggtgcttt catgcgcta 
        agggcgatt ttatggaag gggggacga aaattttca 
        aggagaaat tgttggatc ccgacgatg aaatgcgct 
        tgaaatgtg actgtagag ggattaaaa ttgaagagg 
        aatgtaaag atctgccca aatatgctt cgtgtggtt 
        acttacagt tccggtcat cgctatttc gtgatataa 
        catacaatc tgttaactg ataaccaaa agaaagagt 
        ttcatcaac gtgccatca ggtttctcg aacgtgaaa 
        cacgaaagt gtcaagctt gtggtggtg gttttttcc 
        atcgaattt tgggcgccc accatgaca aggagttta 
        tacgagcca gagacaagc aaaagaaaa ctttttccg 
        gagtcacct catgggagg gcgtaagtc atcgaacat 
        caatgcaca gcatgtcca tacgttccg agagccact 
        tcagtgcgc acagccaaa gttaccaaa gttttcata 
        gtaaaagat ccagattcg attcggtgg cgggaggat 
        cgatggggt caatgccta gccaaattt ttggcgaaa 
        tcaaggagg acaaatcct acgatgcat ttttcggct 
        tttcgataa tcaacaaat tgaaaaaca gtttttcct 
        tgacaaaag ccttctcct cggaggatt ttacagact 
        gccagtgta accttattt tgagcacga cttatgccc 
        agttgattg agcactgca agggggcgg gagatttgg 
        gcaaggaga ttgaggcgt tgctggaat acgcgcaaa 
        tttgcataa tcgcgtcaa atctcccgc ttgcgatgt 
        ccattctgg acgaatcct tggacgtac tgcgagaga 
        aagcgaagg aacgagctg aaaacatat ggaaggtgg 
        cactaatcc gtgctaaga tctactgtc tgattggtt 
        actcgagac tagagctgg ggcacttgg acgaggcct 
        tgctgagtc taaatttat cgttgaacg aatacggga 
        tcacgtttc caaaaggga ttaacaagc acttgaagc 
        tgtatgcgc ataaagtca agcagttgt ttggtttat 
        atctctttc ccaaatctt tgaccaaga acttggatc 
        actgggaaa ccatctttg ctaataacc ttgccgaat 
        gtttgtcgc atttctgca cgctattta tgattaaaa 
        aagagtgga agaatgccg aaatggctg cagtttggg 
        gcttgacca aatctttgg aatctttca gtgactttc 
        gcgtaattt gttcagctt gtgcaaata tgatacatg 
        ttgctcaat attttcaca attggagtt tatgtgttg 
        ctttagaag tgcacgctt tgagactgt gccatttga 
        aaagagctt caatcgaag ccgaggata tcaagtgcg 
        tgtgcaagc tgtattgcg tgatttttg tttcaaggg 
        ttttcatgg cgaatttgg acaaagcca tccacttgg 
        gctacatcg ttctgagat tatgttcat cagtgcatg 
        ccgttcgag tgattcttc ccttactcc cgaccctga 
        ttcagtgcc ttataatgg ggaagcact ttgctaatg 
        cgtattctg tccttcaag agctcaaat tatttgcag 
        aggacgata cggataact caggaaaaa tatggtact 
        acgcgattt gtgttggtt tacaactcg aaacaggct 
        acactttgg tcccgtatc acatatgtg aacatttct 
        atcaacttg aaggcgaaa actgggtta atattagag 
        attcggatg cgagaagac gattcttta ccgtaatgt 
        ggttgtggt gtattagaa atgaaaagt gggaagctg 
        cgtatattt ggtgttaca agacgtctg attttatga 
        gtgtttccg aaaaagatt ctttggcga ataaaataa 
        cttcgttgt atgtgttgg aagggttgt tgctgtatt 
        tcgtaaaca agttagaca aacgtaata ttaaagcta 
        ttatcaggg tggaacacg agataaact cgttgttca 
        agactattg agaagattt ccaaaacag ttaaggttc 
        tcataccga ggttatatc acctgaaag cttattaca 
        agtacataa agatgagtg attctaatg ccattgata 
        tgttaacta aagcgagta attatgctc ataaatatg 
        gaaactctt tagaagaag ggtggattg accattccg 
        taaaggctt ggaataaga cacttcaag ctaatgcaa 
        tagagtttt ggaaagaac attcagaag ctgagaatg 
        tactcgatt ttcaagaca tagaaatta cgggcgaaa 
        ccttgggat attacacat taccgactc cactctatg 
        aataaaaca tttgcagtg tttactgtc atttcgaac 
        taatatata cccaaatcc atg
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