Status
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Gene information
General information
TC033177
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013266,TC013265
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11343204..11359849
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033177
      iBeetle screen iB_05407

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033177 RA
        MMEGMNQVG QEEYIWAMR TSAYRESDS ELWKERRSH 
        SQMMSKKSK SVMDLSIDD RQTKVMASL VPDEVIYNV 
        DDYSHRQYD ACFLFGDVS GFTELCEKY TKTGVSGPS 
        RMTQVLNKY LGSMVQEVL SHNGDVLKF SGDAFLAMF 
        KSSESDTMR DAVHEALDS ALVIQKNYG SYLTDVGVV 
        IRVKLAIST GLVTFALIG DSTNSHYVV VGKPIWDVK 
        AAESISSAG DIVVAPAAW YYVNTNEYV FKEMPDNVH 
        IRLLGVGPN WRSVQRNVR QQKPQDARD DLSTRSEIS 
        ISSDIINDV TQEGIADEF SLRPAVNLA VRLKIKENL 
        RRFIIAPVM KSIDMDEPF EYLTEMRQA VIVFINVIS 
        YKLQTEETV VLADQTFKI VCRVVDKMK GCVNKVSLF 
        DKDLMFVVI FGLRGLKHE MECQVALRC ARECFEEIN 
        KLQGVLSTS IGVTTGKTY CGVFGHTLR REYTVISLI 
        VNKAARLMV AYKNKVTCD RETFLHSKL EARHFILQE 
        HKQLKGISH AGPVYEFRE TELAHDQIQ RINACPLLG 
        RVSKLKLYQ HLLEQCTHS STGHEQYKM YVVRGDPRQ 
        GKTRLLDEF VYITPSHIP VHKLTMAMK DQKIPYQAI 
        RLIFNAPLQ LTPWAPIKE KEQKLLEAL RQIQVPDLM 
        CTLNNVFGV KFKNSELYL SLTETAKLV ALKKMIKQL 
        CYACFTTLW VIAIDDCEF IDDDSWELM ATFLDVKTV 
        FIVGTMGLY RHGHLSYLD DPRIKVVHL APIERWCLG 
        ALACQILDV YAISPELEN VIQTKSNGN PGWVESFLT 
        TLIQDNALY ILKASLKDI NELGLVCPP LYMMTRLSK 
        DETIKWQHI MEERRRSMD SLTERDDWK RFIDSCRDS 
        YLNVTIRER MEDLSRGGK VPICVITPN FRVGEEEGE 
        LSMDAMILK TFDSLNFFE QLLVKCSAI LGNQFLRSM 
        LLYVMSAQD KRKAAQAIQ RLFEIKVLT CAKGDFLED 
        TGVFKERLV DPSDETTLN CKCRGIKIE ENCSDLPRY 
        ASCGYLQFR SSLFREITY NLLTDNQKK EFHSRAIRF 
        LERETRKCK SCGNGFFDR ILGARQDKE FLTARDRQK 
        RTSVSGSWD TLSTSRRNS LRSVSTFSA GSQKSQKKI 
        RYDEKSIAS HSTIFGEGK PGRDKIYTE TFGLTVINK 
        LKHSYSLTR AFSAEDFTD CQCQLILST TYGQLIEHC 
        MGANDFNKV LDAMLEYAY ISITTCNIP LAMKILIEA 
        LDVLRQKEK MRTENKWKL SLIRGQVYT LIGYSRLEL 
        GSFNEAFNM LHRALAEYG ITFPKGVSK QLRTCINEF 
        RQIFGFYLF PRTLMKHLD HWETVFANN LSECLSHLC 
        TLYRIKGEW KNAELAASW SLVKSFESF SDFQVICTA 
        CANMLYVAQ HFQKWNLCI ALEVHSLRL CHLKRTSVE 
        AEDLKCVAK LYGVIFCAR MLRSDLDKA IHIGYIVLR 
        MCSSIRAVK IVLPLLPLL LHALLIGKH FSNAYSILQ 
        ELEYFASED SDHSGKVWY LSLCICLQL ETGYTVLPY 
        QACERFYQI EGEKWITIR DPDARKRFF TVMWLWCVR 
        HEKWEAASI WCIKSNDFS VTFEGDTLA NKVTALYLL 
        EGLILFLVT KLDKRNIKA VTRAEHEIE ELIRLLSKA 
        AERNKIILA RLYHLKAYY KYVKSNDAK VTKLLLRAN 
        KFAEKYGNK LEASWIDHS NRAWNKSLA PIAIDFWKE 
        HCESENFLD FQDIEISEG KLGYFTLPT PVYI
      CDS sequence
      • >TC033177 RA
        atgatggaa gggatgaat caggtgggg caggaggag 
        tacatctgg gccatgaga acaagtgca tacagagaa 
        tcggactca gaattgtgg aaggagcgg cgatctcac 
        agccaaatg atgagcaaa aagtccaag agtgtaatg 
        gatctttca atcgatgat cgacagacc aaagtcatg 
        gcgtctttg gtccccgat gaggtcatc tataacgtg 
        gacgactac agccatcgg caatacgac gcctgtttc 
        ctcttcggg gacgtttcg ggtttcacc gaactgtgc 
        gaaaaatac acaaaaact ggcgtttct ggcccttct 
        cgaatgacc caagtcctc aataaatat ctgggctca 
        atggtccaa gaagtgttg tcccacaac ggcgacgtt 
        ttgaaattc tctggtgac gctttcctt gcaatgttc 
        aaatcgagc gaaagcgac accatgcgg gacgccgtc 
        cacgaagct ctagacagt gctctagtc attcagaaa 
        aattacggt tcgtattta acggacgtc ggtgtcgtg 
        attcgagtg aaattggca atttcgacc ggtcttgtg 
        acctttgcc ctaattgga gactccacc aattctcac 
        tacgtggtg gtgggaaaa ccgatttgg gacgttaaa 
        gctgccgaa agcattagc tcggctggg gacattgtc 
        gtggctccg gcggcgtgg tactacgtc aataccaac 
        gaatacgtc ttcaaggaa atgccagat aatgtccac 
        atccgcttg ctgggtgtt ggtccaaac tggaggagt 
        gtgcaaaga aatgtgcgc caacagaag ccacaagat 
        gccagagat gatttgagt acgcggtcg gaaatctcc 
        atctccagc gatatcata aacgatgtc acccaggaa 
        gggatagca gatgagttt tcactgaga ccagcggtg 
        aatttggcc gtcagactc aagataaaa gaaaacctt 
        cgacggttc atcattgcg ccagtaatg aagagcatc 
        gacatggac gaacctttt gaatatctc acagaaatg 
        cgtcaggcc gttatagta ttcatcaac gttatttct 
        tacaagctc caaaccgaa gaaacggtc gttttggcc 
        gaccaaact tttaaaatt gtttgcagg gtggtagac 
        aagatgaaa ggttgcgta aacaaggtt tctctcttc 
        gataaagac ttgatgttt gttgttatt ttcggcttg 
        cgtggtctt aaacatgaa atggagtgt caggtcgcg 
        ctaagatgt gcgagagag tgtttcgaa gaaatcaac 
        aagcttcaa ggagtttta tccacgtct attggcgtt 
        accacagga aaaacatac tgtggagtc ttcggacac 
        acacttcgc cgcgaatac acagttatc agtcttatt 
        gtgaacaaa gcagctcga ttgatggtg gcctacaaa 
        aataaagta acgtgtgat agggagacg tttctgcac 
        agtaaactg gaggctagg cattttatt ttgcaagaa 
        cacaagcag ctgaaaggg atttcccat gctgggccg 
        gtttatgaa tttcgggag actgagctg gcccacgac 
        caaattcaa cgcataaac gcttgtccg cttttgggc 
        agagtttcc aagttgaaa ttataccaa catctcctg 
        gagcaatgc acccactct tcaactgga cacgagcag 
        tacaaaatg tatgtggta cgcggggat ccccgtcag 
        ggcaaaacc cgacttttg gacgagttt gtgtatata 
        accccctcg catatcccc gttcataaa ctaacaatg 
        gcgatgaag gaccagaaa attccttat caagccata 
        cgacttatc tttaatgcc cccttgcaa ctaaccccc 
        tgggcaccg atcaaagaa aaagaacag aaacttttg 
        gaagcactg agacaaatc caagtcccc gaccttatg 
        tgtactttg aacaacgtg ttcggggtc aaattcaaa 
        aactctgaa ctgtatttg tcactaaca gaaaccgca 
        aaattggta gctttgaaa aaaatgatc aaacagctg 
        tgttatgct tgttttaca acactttgg gtgattgcg 
        atcgacgat tgtgaattt atagatgat gattcgtgg 
        gagcttatg gccacgttt ttggatgtt aaaaccgtt 
        ttcatagtt ggtacaatg ggtttgtat cgacatgga 
        cacttgtca tatcttgac gatcctcga atcaaggtc 
        gtacatctt gctccgatt gaaagatgg tgtttgggg 
        gctctggcc tgtcaaatc ttggacgtt tatgccatt 
        tctcccgag ttggagaat gttattcaa acgaaaagt 
        aacggcaat cctggctgg gttgaaagc tttttgact 
        actttaata caagataat gccctgtac atacttaaa 
        gcttctttg aaagatata aacgagctt ggtttagtg 
        tgtccccct ctctacatg atgacaaga ttgtcgaaa 
        gacgaaaca attaaatgg caacacata atggaagaa 
        cggagacgt tcaatggat tcgctcaca gaacgagac 
        gactggaag cgatttatt gacagttgc cgagatagt 
        tacctaaac gtcacgata agagagcga atggaggat 
        ttatctcga ggcggcaaa gttccgatt tgtgtgatc 
        acacccaac tttcgtgtg ggtgaagaa gaaggtgag 
        ctatcaatg gatgcaatg attttaaaa accttcgat 
        tcgctcaac tttttcgaa caactcttg gtcaaatgt 
        agcgctatt ttgggcaac cagtttctc cgcagcatg 
        cttttgtac gtcatgtcg gcgcaggat aaaagaaaa 
        gcggcgcaa gcaatccag cgcttattt gagataaaa 
        gtcctaacg tgtgctaag ggcgacttc cttgaggac 
        actggcgtt ttcaaagag cgattagtt gatccaagc 
        gatgaaacg accctgaat tgtaaatgc agagggatc 
        aaaatcgag gaaaattgt tccgacctt ccgagatac 
        gcttcgtgt ggttacttg caatttcgc tcctctttg 
        ttccgcgaa atcacctat aacctacta acggataac 
        caaaagaag gagtttcat tcacgagct atccgattt 
        ctggaacgc gaaacgcga aaatgtaaa tcttgtggt 
        aacggtttt tttgaccga atcctcggt gccagacaa 
        gataaggag tttttaacc gctcgggat aggcaaaaa 
        aggacctct gtttcgggt tcttgggat actttgagt 
        acaagtcgg cgcaattcg ttacgaagt gtatccact 
        ttttcggcc ggaagccag aaaagccag aaaaaaatc 
        agatatgat gagaaatca atagcttcg catagcact 
        attttcggg gagggtaaa cctggccgt gacaaaatc 
        tacaccgaa acttttgga ttaacggtt attaataag 
        ctgaaacat agctattcc ttgacgagg gcgttttcg 
        gctgaggac tttaccgat tgccagtgc cagttgatt 
        ttgagtacg acttatggc cagttgatt gagcactgt 
        atgggggcg aatgatttt aataaagtc ctggatgcg 
        atgttggaa tacgcttac attagcata accacttgc 
        aacattcct cttgccatg aaaattcta atcgaggcg 
        ttagacgtt ctaagacag aaggagaaa atgagaact 
        gaaaacaaa tggaaactg tcgctaatt cgtggccaa 
        gtttatact ctaattggt tactctcgc ctagaactt 
        ggcagcttc aacgaggcc tttaatatg cttcacagg 
        gccttggcc gaatatgga attacgttt ccaaaaggt 
        gttagtaag caactgaga acttgcata aacgaattt 
        cgccaaatt ttcggcttc tatttgttt ccacgcact 
        ttgatgaaa catttggac cattgggaa acagttttt 
        gccaataat ctttcagag tgtttgtcg catctttgc 
        actttgtac aggataaag ggtgaatgg aagaacgca 
        gagttggcc gcaagttgg agcttggtt aagtcgttt 
        gagtccttc agtgacttt caagttatt tgcacggct 
        tgtgctaat atgttgtac gtggctcag catttccaa 
        aaatggaat ttatgcatt gcattggaa gttcactcg 
        ttacgtctg tgccacttg aaaaggact tcagtggag 
        gctgaggat ctcaagtgt gttgctaaa ctctatgga 
        gtcattttt tgtgcaaga atgctccga tctgacttg 
        gacaaagca atacatatt ggttacata gtcttgaga 
        atgtgttct tccattcgt gctgttaaa atcgtcctg 
        cccttgctt cccctcttg cttcatgct cttttgatt 
        gggaaacac ttcagcaat gcgtattcc attcttcaa 
        gaactggaa tactttgct tcagaagat tcggaccat 
        tctggcaaa gtgtggtac ttgtctttg tgtatctgt 
        ctgcaactt gagactgga tacactgtc ctcccttac 
        caggcttgc gaacgcttt taccagatt gaaggtgag 
        aaatggata actatccga gatcctgat gccaggaaa 
        aggtttttc actgtaatg tggctttgg tgtgtgcga 
        cacgagaag tgggaagca gcatccatc tggtgtatc 
        aagtccaat gacttttct gttaccttc gaaggtgac 
        actttggcc aacaaagtt acagcgctt tacttgctg 
        gaaggttta attttgttc ctggtaacg aaattagat 
        aaaaggaat ataaaagct gttacaaga gccgaacac 
        gagattgag gaattaatt agactgctt agcaaagca 
        gcggaaaga aataaaatt attttagcc agactctac 
        cacctgaaa gcgtactac aaatatgtg aagagtaac 
        gacgcaaaa gtgaccaag ttgttgctc agggcgaat 
        aaattcgct gagaagtat ggaaataaa ttggaagct 
        tcttggatt gatcacagt aatagagcg tggaataaa 
        tctttggct cctatagcc atcgacttc tggaaggaa 
        cattgcgaa tcagagaac tttctggat tttcaagat 
        attgagatc agtgaggga aaattggga tattttact 
        ctgcctaca cctgtgtac atttag
      mRNA sequence
      • >TC033177 RA
        tttctatag taaaaatgt acttttacg tagtaggac 
        atctgtcat tgccatttt tggcaattt ttacatttt 
        tcttatata aacggtttt atcgaaaaa tcctcaaaa 
        atgatggaa gggatgaat caggtgggg caggaggag 
        tacatctgg gccatgaga acaagtgca tacagagaa 
        tcggactca gaattgtgg aaggagcgg cgatctcac 
        agccaaatg atgagcaaa aagtccaag agtgtaatg 
        gatctttca atcgatgat cgacagacc aaagtcatg 
        gcgtctttg gtccccgat gaggtcatc tataacgtg 
        gacgactac agccatcgg caatacgac gcctgtttc 
        ctcttcggg gacgtttcg ggtttcacc gaactgtgc 
        gaaaaatac acaaaaact ggcgtttct ggcccttct 
        cgaatgacc caagtcctc aataaatat ctgggctca 
        atggtccaa gaagtgttg tcccacaac ggcgacgtt 
        ttgaaattc tctggtgac gctttcctt gcaatgttc 
        aaatcgagc gaaagcgac accatgcgg gacgccgtc 
        cacgaagct ctagacagt gctctagtc attcagaaa 
        aattacggt tcgtattta acggacgtc ggtgtcgtg 
        attcgagtg aaattggca atttcgacc ggtcttgtg 
        acctttgcc ctaattgga gactccacc aattctcac 
        tacgtggtg gtgggaaaa ccgatttgg gacgttaaa 
        gctgccgaa agcattagc tcggctggg gacattgtc 
        gtggctccg gcggcgtgg tactacgtc aataccaac 
        gaatacgtc ttcaaggaa atgccagat aatgtccac 
        atccgcttg ctgggtgtt ggtccaaac tggaggagt 
        gtgcaaaga aatgtgcgc caacagaag ccacaagat 
        gccagagat gatttgagt acgcggtcg gaaatctcc 
        atctccagc gatatcata aacgatgtc acccaggaa 
        gggatagca gatgagttt tcactgaga ccagcggtg 
        aatttggcc gtcagactc aagataaaa gaaaacctt 
        cgacggttc atcattgcg ccagtaatg aagagcatc 
        gacatggac gaacctttt gaatatctc acagaaatg 
        cgtcaggcc gttatagta ttcatcaac gttatttct 
        tacaagctc caaaccgaa gaaacggtc gttttggcc 
        gaccaaact tttaaaatt gtttgcagg gtggtagac 
        aagatgaaa ggttgcgta aacaaggtt tctctcttc 
        gataaagac ttgatgttt gttgttatt ttcggcttg 
        cgtggtctt aaacatgaa atggagtgt caggtcgcg 
        ctaagatgt gcgagagag tgtttcgaa gaaatcaac 
        aagcttcaa ggagtttta tccacgtct attggcgtt 
        accacagga aaaacatac tgtggagtc ttcggacac 
        acacttcgc cgcgaatac acagttatc agtcttatt 
        gtgaacaaa gcagctcga ttgatggtg gcctacaaa 
        aataaagta acgtgtgat agggagacg tttctgcac 
        agtaaactg gaggctagg cattttatt ttgcaagaa 
        cacaagcag ctgaaaggg atttcccat gctgggccg 
        gtttatgaa tttcgggag actgagctg gcccacgac 
        caaattcaa cgcataaac gcttgtccg cttttgggc 
        agagtttcc aagttgaaa ttataccaa catctcctg 
        gagcaatgc acccactct tcaactgga cacgagcag 
        tacaaaatg tatgtggta cgcggggat ccccgtcag 
        ggcaaaacc cgacttttg gacgagttt gtgtatata 
        accccctcg catatcccc gttcataaa ctaacaatg 
        gcgatgaag gaccagaaa attccttat caagccata 
        cgacttatc tttaatgcc cccttgcaa ctaaccccc 
        tgggcaccg atcaaagaa aaagaacag aaacttttg 
        gaagcactg agacaaatc caagtcccc gaccttatg 
        tgtactttg aacaacgtg ttcggggtc aaattcaaa 
        aactctgaa ctgtatttg tcactaaca gaaaccgca 
        aaattggta gctttgaaa aaaatgatc aaacagctg 
        tgttatgct tgttttaca acactttgg gtgattgcg 
        atcgacgat tgtgaattt atagatgat gattcgtgg 
        gagcttatg gccacgttt ttggatgtt aaaaccgtt 
        ttcatagtt ggtacaatg ggtttgtat cgacatgga 
        cacttgtca tatcttgac gatcctcga atcaaggtc 
        gtacatctt gctccgatt gaaagatgg tgtttgggg 
        gctctggcc tgtcaaatc ttggacgtt tatgccatt 
        tctcccgag ttggagaat gttattcaa acgaaaagt 
        aacggcaat cctggctgg gttgaaagc tttttgact 
        actttaata caagataat gccctgtac atacttaaa 
        gcttctttg aaagatata aacgagctt ggtttagtg 
        tgtccccct ctctacatg atgacaaga ttgtcgaaa 
        gacgaaaca attaaatgg caacacata atggaagaa 
        cggagacgt tcaatggat tcgctcaca gaacgagac 
        gactggaag cgatttatt gacagttgc cgagatagt 
        tacctaaac gtcacgata agagagcga atggaggat 
        ttatctcga ggcggcaaa gttccgatt tgtgtgatc 
        acacccaac tttcgtgtg ggtgaagaa gaaggtgag 
        ctatcaatg gatgcaatg attttaaaa accttcgat 
        tcgctcaac tttttcgaa caactcttg gtcaaatgt 
        agcgctatt ttgggcaac cagtttctc cgcagcatg 
        cttttgtac gtcatgtcg gcgcaggat aaaagaaaa 
        gcggcgcaa gcaatccag cgcttattt gagataaaa 
        gtcctaacg tgtgctaag ggcgacttc cttgaggac 
        actggcgtt ttcaaagag cgattagtt gatccaagc 
        gatgaaacg accctgaat tgtaaatgc agagggatc 
        aaaatcgag gaaaattgt tccgacctt ccgagatac 
        gcttcgtgt ggttacttg caatttcgc tcctctttg 
        ttccgcgaa atcacctat aacctacta acggataac 
        caaaagaag gagtttcat tcacgagct atccgattt 
        ctggaacgc gaaacgcga aaatgtaaa tcttgtggt 
        aacggtttt tttgaccga atcctcggt gccagacaa 
        gataaggag tttttaacc gctcgggat aggcaaaaa 
        aggacctct gtttcgggt tcttgggat actttgagt 
        acaagtcgg cgcaattcg ttacgaagt gtatccact 
        ttttcggcc ggaagccag aaaagccag aaaaaaatc 
        agatatgat gagaaatca atagcttcg catagcact 
        attttcggg gagggtaaa cctggccgt gacaaaatc 
        tacaccgaa acttttgga ttaacggtt attaataag 
        ctgaaacat agctattcc ttgacgagg gcgttttcg 
        gctgaggac tttaccgat tgccagtgc cagttgatt 
        ttgagtacg acttatggc cagttgatt gagcactgt 
        atgggggcg aatgatttt aataaagtc ctggatgcg 
        atgttggaa tacgcttac attagcata accacttgc 
        aacattcct cttgccatg aaaattcta atcgaggcg 
        ttagacgtt ctaagacag aaggagaaa atgagaact 
        gaaaacaaa tggaaactg tcgctaatt cgtggccaa 
        gtttatact ctaattggt tactctcgc ctagaactt 
        ggcagcttc aacgaggcc tttaatatg cttcacagg 
        gccttggcc gaatatgga attacgttt ccaaaaggt 
        gttagtaag caactgaga acttgcata aacgaattt 
        cgccaaatt ttcggcttc tatttgttt ccacgcact 
        ttgatgaaa catttggac cattgggaa acagttttt 
        gccaataat ctttcagag tgtttgtcg catctttgc 
        actttgtac aggataaag ggtgaatgg aagaacgca 
        gagttggcc gcaagttgg agcttggtt aagtcgttt 
        gagtccttc agtgacttt caagttatt tgcacggct 
        tgtgctaat atgttgtac gtggctcag catttccaa 
        aaatggaat ttatgcatt gcattggaa gttcactcg 
        ttacgtctg tgccacttg aaaaggact tcagtggag 
        gctgaggat ctcaagtgt gttgctaaa ctctatgga 
        gtcattttt tgtgcaaga atgctccga tctgacttg 
        gacaaagca atacatatt ggttacata gtcttgaga 
        atgtgttct tccattcgt gctgttaaa atcgtcctg 
        cccttgctt cccctcttg cttcatgct cttttgatt 
        gggaaacac ttcagcaat gcgtattcc attcttcaa 
        gaactggaa tactttgct tcagaagat tcggaccat 
        tctggcaaa gtgtggtac ttgtctttg tgtatctgt 
        ctgcaactt gagactgga tacactgtc ctcccttac 
        caggcttgc gaacgcttt taccagatt gaaggtgag 
        aaatggata actatccga gatcctgat gccaggaaa 
        aggtttttc actgtaatg tggctttgg tgtgtgcga 
        cacgagaag tgggaagca gcatccatc tggtgtatc 
        aagtccaat gacttttct gttaccttc gaaggtgac 
        actttggcc aacaaagtt acagcgctt tacttgctg 
        gaaggttta attttgttc ctggtaacg aaattagat 
        aaaaggaat ataaaagct gttacaaga gccgaacac 
        gagattgag gaattaatt agactgctt agcaaagca 
        gcggaaaga aataaaatt attttagcc agactctac 
        cacctgaaa gcgtactac aaatatgtg aagagtaac 
        gacgcaaaa gtgaccaag ttgttgctc agggcgaat 
        aaattcgct gagaagtat ggaaataaa ttggaagct 
        tcttggatt gatcacagt aatagagcg tggaataaa 
        tctttggct cctatagcc atcgacttc tggaaggaa 
        cattgcgaa tcagagaac tttctggat tttcaagat 
        attgagatc agtgaggga aaattggga tattttact 
        ctgcctaca cctgtgtac atttagcta caaataaaa 
        acctttgaa ttga
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