Status
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Gene information
General information
TC033180
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013262
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11436990..11457382
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033180
    iBeetle screen iB_05406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033180 RA
      MQGQDNLSS SGDHRLALI HEMSAHNFD LIRFASYRT 
      ATKLRFIQK KVSLHAVDI WNVIEAFRE QGLHALEPS 
      SELSVARLE TLLCSLYHS LNKRAPPTQ QAHVDVCSS 
      LLLNWLLAA YATVDNVGK IRVFSIKVA LATLCAGKL 
      MDKLRYIFS QISDSNGLL IQWRFNEYL QEVLALPAA 
      VYESPTFNY TDSLANSIF NPNVKITVN DFLDTLMSD 
      PGPPCLVWL PLLHRIANV ENVIHPTQC DACQRENFS 
      GFRYRCQKC PHYTLCQDC FWKGRVTAP HTLDHQVKE 
      YSSFSPSKT IGQSLRKSF RCVPDKQKN NLPRFPEQP 
      EKTLNLSHI VPPSPIPSH NGFPDGNNF TSHFDGMGS 
      LDSRSTARS LDSARDDEH KMLARYAAR LAQEARSGM 
      ARSGYRKNS LSPDTGRVP SEACLGMDS TRAQRELIS 
      QLEAKNREI MREIARLRR QQELENSGH GTENPALMN 
      ELRALRMRK DELETHLTT LQDSRRQLM IQLEGLMKM 
      LKNHQSSPR STPNSSPRS TKSPPLLPG GAPATQPPR 
      SAPQTPMSM PPTNNSIVT VSQAERPTH VGHPMGGIP 
      TSVQERSER VMHNSDSLM GVGGDVRNA FGGASNVSM 
      GSNGNGTPG SVGRSLRND LLVAADSVT NAMSTLVRE 
      LNSEPSNQP EASVIRKPL DFEDDGEDE SDGGWQQEL 
      QRRYAQDAN FMAELRARH PTSPPENHP SQNYNHHQY 
      PALNHHHPN QGYNAQQNY PNYNQAEPN QSPFGPQTP 
      KSEQQSVLQ QQHEQNNDN GEEIPNREG MSKETLQFE 
      EALKQWVNR
    • >TC033180 RB
      MQGQDNLSS SGDHRLALI HEMSAHNFD LIRFASYRT 
      ATKLRFIQK KVSLHAVDI WNVIEAFRE QGLHALEPS 
      SELSVARLE TLLCSLYHS LNKRAPPTQ QAHVDVCSS 
      LLLNWLLAA YATVDNVGK IRVFSIKVA LATLCAGKL 
      MDKLRYIFS QISDSNGLL IQWRFNEYL QEVLALPAA 
      VYESPTFNY TDSLANSIF NPNVKITVN DFLDTLMSD 
      PGPPCLVWL PLLHRIANV ENVIHPTQC DACQRENFS 
      GFRYRCQKC PHYTLCQDC FWKGRVTAP HTLDHQVKE 
      YSSFSPSKT IGQSLRKSF RCVPDKQKN NLPRFPEQP 
      EKTLNLSHI VPPSPIPSH NGFPDGNNF TSHFDGMGS 
      LDSRSTARS LDSARDDEH KMLARYAAR LAQEARSGM 
      ARSGYRKNS LSPDTGRVP SEACLGMDS TRAQRELIS 
      QLEAKNREI MREIARLRR QQELENSGH GTENPALMN 
      ELRALRMRK DELETHLTT LQDSRRQLM IQLEGLMKM 
      LKNHQSSPR STPNSSPRS TKSPPLLPG GAPATQPPR 
      SAPQTPMSM PPTNNSIVT VSQAERPTH VGHPMGGIP 
      TSVQERSER VMHNSDSLM GVGGDVRNA FGGASNVSM 
      GSNGNGTPG SVGRSLRND LLVAADSVT NAMSTLVRE 
      LNSGTDM
    • >TC033180 RC
      MTVKTRVTA AGSRNCSVA TPKTPTSWR SCAHGTPQA 
      PRKTTPAKI TTTTSTLPL TTTTPTKAT MPNKITPIT 
      IRPSPIKAR SDRRWVPPS DNTTASCAF RETPKSEQQ 
      SVLQQQHEQ NNDNGEEIP NREGMSKET LQFEEALKQ 
      WVNR
    CDS sequence
    • >TC033180 RA
      atgcaggga caagacaac ctttcgagt tcgggggac 
      caccggttg gccttaatt cacgagatg agtgcccac 
      aatttcgat ctgatccga tttgcctca taccgaact 
      gccacaaaa ctcagattt attcagaaa aaagtcagc 
      ctacatgct gttgatatt tggaatgtg atagaagcg 
      ttccgggag cagggcctc cacgccttg gagccctcc 
      tcagaactt agtgttgcc cggcttgag acccttctg 
      tgttcgctt taccacagt ctgaacaaa agggctcca 
      cctacgcaa caagcgcac gtcgatgtg tgctccagt 
      ttactgctg aattggctt ctggccgct tatgccact 
      gtagataat gtgggaaaa atccgtgtt ttttccatt 
      aaggtagcc ctggcaaca ttatgtgcc ggaaaatta 
      atggataaa ttgcgatac attttttcc caaatatcc 
      gacagtaac ggtctcctc attcaatgg cgtttcaac 
      gaatacctt caagaagtg ttggctttg cctgcagcc 
      gtttacgaa tctcccacg ttcaattat accgattcg 
      ctcgcaaat tcaattttc aatcctaac gttaaaatc 
      acagtgaat gactttttg gatactttg atgtccgat 
      cctggacca ccgtgtctg gtgtggttg ccattactt 
      catcgaata gctaatgta gaaaacgtg atacacccg 
      actcagtgt gatgcgtgt caaagggag aatttcagc 
      gggtttcgt taccggtgt cagaagtgc ccccattac 
      actttatgc caggactgc ttctggaaa gggagggtc 
      acggcgccg cacacgctg gatcaccaa gtcaaggaa 
      tactcatct ttttctcct agtaaaaca atcggacag 
      tccctgaga aagtcgttt cgttgcgtg cccgacaag 
      cagaaaaac aacttgccg cgcttcccc gaacagccc 
      gagaaaacc ctcaacctt tcgcacatc gtgccccct 
      tcaccgatc ccgtctcac aatggcttc cccgatggt 
      aacaatttc acgtcgcat ttcgacgga atgggtagt 
      ttggatagc agatcgacg gcgagaagt ttagacagt 
      gcccgagac gatgagcat aaaatgttg gcgaggtac 
      gcggcgcga ttggcccaa gaagcgcgc tctggcatg 
      gcgagaagc ggttaccga aaaaactcg ctttctccg 
      gatacgggg agagttcct tcagaggct tgtttagga 
      atggactcg acgcgggcc caacgcgag ttaatcagc 
      caattggaa gcgaaaaat cgcgaaatt atgcgagaa 
      atcgccagg ttaaggcgc caacaagag ttggaaaac 
      agcggccac ggcaccgaa aatccggcc ctaatgaac 
      gaactcaga gcgttacgt atgcgtaaa gacgaactc 
      gagacccac ttaaccacc ttgcaggat tcgcgtcgt 
      cagctgatg atccagctt gaaggacta atgaaaatg 
      ttgaaaaat caccagagt tctccacgg tccacaccg 
      aactcatct ccacgaagc acaaagtct cctccgtta 
      cttccaggt ggcgctcca gccacacaa ccgcctcgt 
      agtgcccca cagacgccc atgagtatg cctcctacc 
      aataattcc attgtgact gttagtcaa gcggaacgg 
      ccgacccat gtgggacac cccatggga gggattccc 
      acttctgtc caggaaaga agtgagagg gttatgcat 
      aatagcgat tctttgatg ggggttgga ggggacgtg 
      aggaatgcg ttcgggggg gcttccaat gtcagcatg 
      ggttccaac ggaaatggg acgccgggc tccgtggga 
      cgatcgttg aggaacgat ttgttagtg gctgcggat 
      tctgtaact aacgcaatg tcaacttta gtaagagaa 
      ctcaattca gaaccttcg aatcagcca gaagctagc 
      gtaatcaga aaaccttta gatttcgaa gatgacggt 
      gaagacgag agtgacggc ggctggcag caggaactg 
      cagcgtcgc tacgcccaa gacgccaac ttcatggcg 
      gagttgcgc gcacggcac cccacaagc cccccggaa 
      aaccacccc agccaaaat tacaaccac caccagtac 
      cctgccctt aaccaccac caccccaac caaggctac 
      aatgcccaa caaaattac cccaattac aatcaggcc 
      gagcccaat caaagcccg ttcggaccg cagaccccg 
      aagagtgaa caacagtct gtcttgcaa cagcaacat 
      gaacaaaac aacgacaac ggcgaagaa attccgaac 
      cgagaaggg atgagcaaa gaaaccctc cagtttgaa 
      gaagcacta aagcaatgg gtcaatcga taa
    • >TC033180 RB
      atgcaggga caagacaac ctttcgagt tcgggggac 
      caccggttg gccttaatt cacgagatg agtgcccac 
      aatttcgat ctgatccga tttgcctca taccgaact 
      gccacaaaa ctcagattt attcagaaa aaagtcagc 
      ctacatgct gttgatatt tggaatgtg atagaagcg 
      ttccgggag cagggcctc cacgccttg gagccctcc 
      tcagaactt agtgttgcc cggcttgag acccttctg 
      tgttcgctt taccacagt ctgaacaaa agggctcca 
      cctacgcaa caagcgcac gtcgatgtg tgctccagt 
      ttactgctg aattggctt ctggccgct tatgccact 
      gtagataat gtgggaaaa atccgtgtt ttttccatt 
      aaggtagcc ctggcaaca ttatgtgcc ggaaaatta 
      atggataaa ttgcgatac attttttcc caaatatcc 
      gacagtaac ggtctcctc attcaatgg cgtttcaac 
      gaatacctt caagaagtg ttggctttg cctgcagcc 
      gtttacgaa tctcccacg ttcaattat accgattcg 
      ctcgcaaat tcaattttc aatcctaac gttaaaatc 
      acagtgaat gactttttg gatactttg atgtccgat 
      cctggacca ccgtgtctg gtgtggttg ccattactt 
      catcgaata gctaatgta gaaaacgtg atacacccg 
      actcagtgt gatgcgtgt caaagggag aatttcagc 
      gggtttcgt taccggtgt cagaagtgc ccccattac 
      actttatgc caggactgc ttctggaaa gggagggtc 
      acggcgccg cacacgctg gatcaccaa gtcaaggaa 
      tactcatct ttttctcct agtaaaaca atcggacag 
      tccctgaga aagtcgttt cgttgcgtg cccgacaag 
      cagaaaaac aacttgccg cgcttcccc gaacagccc 
      gagaaaacc ctcaacctt tcgcacatc gtgccccct 
      tcaccgatc ccgtctcac aatggcttc cccgatggt 
      aacaatttc acgtcgcat ttcgacgga atgggtagt 
      ttggatagc agatcgacg gcgagaagt ttagacagt 
      gcccgagac gatgagcat aaaatgttg gcgaggtac 
      gcggcgcga ttggcccaa gaagcgcgc tctggcatg 
      gcgagaagc ggttaccga aaaaactcg ctttctccg 
      gatacgggg agagttcct tcagaggct tgtttagga 
      atggactcg acgcgggcc caacgcgag ttaatcagc 
      caattggaa gcgaaaaat cgcgaaatt atgcgagaa 
      atcgccagg ttaaggcgc caacaagag ttggaaaac 
      agcggccac ggcaccgaa aatccggcc ctaatgaac 
      gaactcaga gcgttacgt atgcgtaaa gacgaactc 
      gagacccac ttaaccacc ttgcaggat tcgcgtcgt 
      cagctgatg atccagctt gaaggacta atgaaaatg 
      ttgaaaaat caccagagt tctccacgg tccacaccg 
      aactcatct ccacgaagc acaaagtct cctccgtta 
      cttccaggt ggcgctcca gccacacaa ccgcctcgt 
      agtgcccca cagacgccc atgagtatg cctcctacc 
      aataattcc attgtgact gttagtcaa gcggaacgg 
      ccgacccat gtgggacac cccatggga gggattccc 
      acttctgtc caggaaaga agtgagagg gttatgcat 
      aatagcgat tctttgatg ggggttgga ggggacgtg 
      aggaatgcg ttcgggggg gcttccaat gtcagcatg 
      ggttccaac ggaaatggg acgccgggc tccgtggga 
      cgatcgttg aggaacgat ttgttagtg gctgcggat 
      tctgtaact aacgcaatg tcaacttta gtaagagaa 
      ctcaattca ggtacggac atgtaa
    • >TC033180 RC
      atgacggtg aagacgaga gtgacggcg gctggcagc 
      aggaactgc agcgtcgct acgcccaag acgccaact 
      tcatggcgg agttgcgcg cacggcacc ccacaagcc 
      ccccggaaa accacccca gccaaaatt acaaccacc 
      accagtacc ctgccctta accaccacc accccaacc 
      aaggctaca atgcccaac aaaattacc ccaattaca 
      atcaggccg agcccaatc aaagcccgt tcggaccgc 
      aggtgggtg cctccttca gataatacc actgctagc 
      tgcgctttt agagagacc ccgaagagt gaacaacag 
      tctgtcttg caacagcaa catgaacaa aacaacgac 
      aacggcgaa gaaattccg aaccgagaa gggatgagc 
      aaagaaacc ctccagttt gaagaagca ctaaagcaa 
      tgggtcaat cgataa
    mRNA sequence
    • >TC033180 RA
      gaaaatggc gtatgtcgt gtggtcgaa cgtggtgtt 
      tctctcaca tttttaact tttttcagt gatttctta 
      ccgcagttt ctctacata ttcaaaaaa taataagaa 
      aacacaatg cagggacaa gacaacctt tcgagttcg 
      ggggaccac cggttggcc ttaattcac gagatgagt 
      gcccacaat ttcgatctg atccgattt gcctcatac 
      cgaactgcc acaaaactc agatttatt cagaaaaaa 
      gtcagccta catgctgtt gatatttgg aatgtgata 
      gaagcgttc cgggagcag ggcctccac gccttggag 
      ccctcctca gaacttagt gttgcccgg cttgagacc 
      cttctgtgt tcgctttac cacagtctg aacaaaagg 
      gctccacct acgcaacaa gcgcacgtc gatgtgtgc 
      tccagttta ctgctgaat tggcttctg gccgcttat 
      gccactgta gataatgtg ggaaaaatc cgtgttttt 
      tccattaag gtagccctg gcaacatta tgtgccgga 
      aaattaatg gataaattg cgatacatt ttttcccaa 
      atatccgac agtaacggt ctcctcatt caatggcgt 
      ttcaacgaa taccttcaa gaagtgttg gctttgcct 
      gcagccgtt tacgaatct cccacgttc aattatacc 
      gattcgctc gcaaattca attttcaat cctaacgtt 
      aaaatcaca gtgaatgac tttttggat actttgatg 
      tccgatcct ggaccaccg tgtctggtg tggttgcca 
      ttacttcat cgaatagct aatgtagaa aacgtgata 
      cacccgact cagtgtgat gcgtgtcaa agggagaat 
      ttcagcggg tttcgttac cggtgtcag aagtgcccc 
      cattacact ttatgccag gactgcttc tggaaaggg 
      agggtcacg gcgccgcac acgctggat caccaagtc 
      aaggaatac tcatctttt tctcctagt aaaacaatc 
      ggacagtcc ctgagaaag tcgtttcgt tgcgtgccc 
      gacaagcag aaaaacaac ttgccgcgc ttccccgaa 
      cagcccgag aaaaccctc aacctttcg cacatcgtg 
      cccccttca ccgatcccg tctcacaat ggcttcccc 
      gatggtaac aatttcacg tcgcatttc gacggaatg 
      ggtagtttg gatagcaga tcgacggcg agaagttta 
      gacagtgcc cgagacgat gagcataaa atgttggcg 
      aggtacgcg gcgcgattg gcccaagaa gcgcgctct 
      ggcatggcg agaagcggt taccgaaaa aactcgctt 
      tctccggat acggggaga gttccttca gaggcttgt 
      ttaggaatg gactcgacg cgggcccaa cgcgagtta 
      atcagccaa ttggaagcg aaaaatcgc gaaattatg 
      cgagaaatc gccaggtta aggcgccaa caagagttg 
      gaaaacagc ggccacggc accgaaaat ccggcccta 
      atgaacgaa ctcagagcg ttacgtatg cgtaaagac 
      gaactcgag acccactta accaccttg caggattcg 
      cgtcgtcag ctgatgatc cagcttgaa ggactaatg 
      aaaatgttg aaaaatcac cagagttct ccacggtcc 
      acaccgaac tcatctcca cgaagcaca aagtctcct 
      ccgttactt ccaggtggc gctccagcc acacaaccg 
      cctcgtagt gccccacag acgcccatg agtatgcct 
      cctaccaat aattccatt gtgactgtt agtcaagcg 
      gaacggccg acccatgtg ggacacccc atgggaggg 
      attcccact tctgtccag gaaagaagt gagagggtt 
      atgcataat agcgattct ttgatgggg gttggaggg 
      gacgtgagg aatgcgttc gggggggct tccaatgtc 
      agcatgggt tccaacgga aatgggacg ccgggctcc 
      gtgggacga tcgttgagg aacgatttg ttagtggct 
      gcggattct gtaactaac gcaatgtca actttagta 
      agagaactc aattcagaa ccttcgaat cagccagaa 
      gctagcgta atcagaaaa cctttagat ttcgaagat 
      gacggtgaa gacgagagt gacggcggc tggcagcag 
      gaactgcag cgtcgctac gcccaagac gccaacttc 
      atggcggag ttgcgcgca cggcacccc acaagcccc 
      ccggaaaac caccccagc caaaattac aaccaccac 
      cagtaccct gcccttaac caccaccac cccaaccaa 
      ggctacaat gcccaacaa aattacccc aattacaat 
      caggccgag cccaatcaa agcccgttc ggaccgcag 
      accccgaag agtgaacaa cagtctgtc ttgcaacag 
      caacatgaa caaaacaac gacaacggc gaagaaatt 
      ccgaaccga gaagggatg agcaaagaa accctccag 
      tttgaagaa gcactaaag caatgggtc aatcgataa 
      aactagaaa atctctcta taataatac atcttgtcc 
      gtgtactct cattaagta taccagtaa aaaaatatg 
      ttcattagt ggtcctttg actatactg ttgagtgtg 
      tcgaggtct gaaattttt tccgggaaa gaagatggc 
      ttgttagat tccattaat tcgtaggta ctacattaa 
      gaatgtata taaatatgg tattaagtg ctttgcacc 
      catagttcc tttttgtat aagattaag tgatgtgcg 
      caaagattt cggcttaag atagctgga ccaagatac 
      tcctctaat ctatattag ttgacacaa cagtaacca 
      accttcatg cttgtataa catttggaa ctttcatac 
      ttttattaa ccttttcat gaaaagtac atattaact 
      gtaactcgg acttatgta ggtcgttgt tgtaatgtc 
      tagtatata cttgtagta aaaataagg acgattatg 
      acgatggta ttagttatt cgactggtt gttgctttg 
      acattacct agggtatta tggtcaata ctcaattgt 
      acaattttg ttgattatg ttgtttgat tttattgaa 
      tatttacga atgtctgat gtgaactaa ttaataaat 
      gatgaattg gatg
    • >TC033180 RB
      gaaaatggc gtatgtcgt gtggtcgaa cgtggtgtt 
      tctctcaca tttttaact tttttcagt gatttctta 
      ccgcagttt ctctacata ttcaaaaaa taataagaa 
      aacacaatg cagggacaa gacaacctt tcgagttcg 
      ggggaccac cggttggcc ttaattcac gagatgagt 
      gcccacaat ttcgatctg atccgattt gcctcatac 
      cgaactgcc acaaaactc agatttatt cagaaaaaa 
      gtcagccta catgctgtt gatatttgg aatgtgata 
      gaagcgttc cgggagcag ggcctccac gccttggag 
      ccctcctca gaacttagt gttgcccgg cttgagacc 
      cttctgtgt tcgctttac cacagtctg aacaaaagg 
      gctccacct acgcaacaa gcgcacgtc gatgtgtgc 
      tccagttta ctgctgaat tggcttctg gccgcttat 
      gccactgta gataatgtg ggaaaaatc cgtgttttt 
      tccattaag gtagccctg gcaacatta tgtgccgga 
      aaattaatg gataaattg cgatacatt ttttcccaa 
      atatccgac agtaacggt ctcctcatt caatggcgt 
      ttcaacgaa taccttcaa gaagtgttg gctttgcct 
      gcagccgtt tacgaatct cccacgttc aattatacc 
      gattcgctc gcaaattca attttcaat cctaacgtt 
      aaaatcaca gtgaatgac tttttggat actttgatg 
      tccgatcct ggaccaccg tgtctggtg tggttgcca 
      ttacttcat cgaatagct aatgtagaa aacgtgata 
      cacccgact cagtgtgat gcgtgtcaa agggagaat 
      ttcagcggg tttcgttac cggtgtcag aagtgcccc 
      cattacact ttatgccag gactgcttc tggaaaggg 
      agggtcacg gcgccgcac acgctggat caccaagtc 
      aaggaatac tcatctttt tctcctagt aaaacaatc 
      ggacagtcc ctgagaaag tcgtttcgt tgcgtgccc 
      gacaagcag aaaaacaac ttgccgcgc ttccccgaa 
      cagcccgag aaaaccctc aacctttcg cacatcgtg 
      cccccttca ccgatcccg tctcacaat ggcttcccc 
      gatggtaac aatttcacg tcgcatttc gacggaatg 
      ggtagtttg gatagcaga tcgacggcg agaagttta 
      gacagtgcc cgagacgat gagcataaa atgttggcg 
      aggtacgcg gcgcgattg gcccaagaa gcgcgctct 
      ggcatggcg agaagcggt taccgaaaa aactcgctt 
      tctccggat acggggaga gttccttca gaggcttgt 
      ttaggaatg gactcgacg cgggcccaa cgcgagtta 
      atcagccaa ttggaagcg aaaaatcgc gaaattatg 
      cgagaaatc gccaggtta aggcgccaa caagagttg 
      gaaaacagc ggccacggc accgaaaat ccggcccta 
      atgaacgaa ctcagagcg ttacgtatg cgtaaagac 
      gaactcgag acccactta accaccttg caggattcg 
      cgtcgtcag ctgatgatc cagcttgaa ggactaatg 
      aaaatgttg aaaaatcac cagagttct ccacggtcc 
      acaccgaac tcatctcca cgaagcaca aagtctcct 
      ccgttactt ccaggtggc gctccagcc acacaaccg 
      cctcgtagt gccccacag acgcccatg agtatgcct 
      cctaccaat aattccatt gtgactgtt agtcaagcg 
      gaacggccg acccatgtg ggacacccc atgggaggg 
      attcccact tctgtccag gaaagaagt gagagggtt 
      atgcataat agcgattct ttgatgggg gttggaggg 
      gacgtgagg aatgcgttc gggggggct tccaatgtc 
      agcatgggt tccaacgga aatgggacg ccgggctcc 
      gtgggacga tcgttgagg aacgatttg ttagtggct 
      gcggattct gtaactaac gcaatgtca actttagta 
      agagaactc aattcaggt acggacatg taaaaatct 
      aggaaattt tgtgtattt atgtatcta actgaaagc 
      ttacaccgt ttagtatta tgaattact gaggctact 
      tagtttctc ctccacttt ctcgtttta ggttttgta 
      caaagttag tttatatta tttttttac aattttgta 
      tatttcgtc ttgttatac attctgact ttttggttg 
      tatctgtta cgactatga tgatttgtt gtacgataa 
      acacaagtt tgactgagt atgttttaa tttgcggaa 
      tgcgattgt aattggatc tgccgctag catgtgact 
      acatgaaat acaattcaa cactgatt
    • >TC033180 RC
      aatgattgt caaattaca gaaccttcg aatcagcca 
      gaagctagc gtaatcaga aaaccttta gatttcgaa 
      gatgacggt gaagacgag agtgacggc ggctggcag 
      caggaactg cagcgtcgc tacgcccaa gacgccaac 
      ttcatggcg gagttgcgc gcacggcac cccacaagc 
      cccccggaa aaccacccc agccaaaat tacaaccac 
      caccagtac cctgccctt aaccaccac caccccaac 
      caaggctac aatgcccaa caaaattac cccaattac 
      aatcaggcc gagcccaat caaagcccg ttcggaccg 
      caggtgggt gcctccttc agataatac cactgctag 
      ctgcgcttt tagagagac cccgaagag tgaacaaca 
      gtctgtctt gcaacagca acatgaaca aaacaacga 
      caacggcga agaaattcc gaaccgaga agggatgag 
      caaagaaac cctccagtt tgaagaagc actaaagca 
      atgggtcaa tcgataaaa ctagaaaat ctctctata 
      ataatacat cttgtccgt gtactctca ttaagtata 
      ccagtaaaa aaatatgtt cattagtgg tcctttgac 
      tatactgtt gagtgtgtc gaggtctga aattttttc 
      cgggaaaga agatggctt gttagattc cattaattc 
      gtaggtact acattaaga atgtatata aatatggta 
      ttaagtgct ttgcaccca tagttcctt tttgtataa 
      gattaagtg atgtgcgca aagatttcg gcttaagat 
      agctggacc aagatactc ctctaatct atattagtt 
      gacacaaca gtaaccaac cttcatgct tgtataaca 
      tttggaact ttcatactt ttattaacc ttttcatga 
      aaagtacat attaactgt aactcggac ttatgtagg 
      tcgttgttg taatgtcta gtatatact tgtagtaaa 
      aataaggac gattatgac gatggtatt agttattcg 
      actggttgt tgctttgac attacctag ggtattatg 
      gtcaatact caattgtac aattttgtt gattatgtt 
      gtttgattt tattgaata tttacgaat gtctgatgt 
      gaactaatt aataaatga tgaattgga tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY