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Gene information
General information
TC013247 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11558619..11562349
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013247
    iBeetle screen iB_05405

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment:
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013247 RA
      MSYSVVPIE EEKLQENEE NVAKSLYRW IQGFSKACD 
      NNSIKNIAR LQDNKVLKK FMQLDTNCD FSTTEKRNK 
      FKYEHIETV LNKYDMDNK NAKDVTYSM FFASALLDL 
      DKLAKIIKL KPSLIDQTI YSNSVLIFT LKYGNFYKE 
      NFVDCVKEL IESGVNVNK CDLVGKSAI DYVAELYEE 
      NKDNSDVAA KLKTVARLI LNKTVYGND NNMTKNFLE 
      KIMNFDLNT LAEMDVKTK LFYCIVKNK ITELQKLED 
      VEQYLNCDT EENTLLQIA CKRNCTLDV IRWLLNNGA 
      DVNMKTPLN PLTPLELAA THNSIKIFE EILKCRETK 
      IGKDHFNNL IKWHKGSMK SRIFELFLE SDKINVEFK 
      SEGTGNTPL HYAVWFSYK TAIQNLLQC GASLTVKNN 
      NQKCPLDII DSADLEKYF DNCIALDNY NQHFANPDY 
      KMNIRFTSL IKDGELKES TVFSKIGNR PKLEHLLTH 
      PLIHLFILL KWHLIRVYF WTVLLVKAT LYSLLAYCL 
      YHLPGLSAG GYAFCVTLA VINTFIKIP IFLYWQKKG 
      KRNEFLVMQ RAPLHAFLE IFLVIIMWG SFFQNQFRA 
      FVFIALAVS FLLTTGYHP KLSKWSIML QKVFKTFTI 
      LLIFFSLII VAFSAGFQM LFPGEGQFR DFFSAVFKT 
      YIMATDDYN IDEHTDFDS AGIAGYFMF LLFAMSISL 
      VIVNFWTGV AVTDIERIE RTSVVNAFR NVIIFMSFI 
      EWVYRFEFL GKVRGCVPN PLLFPGDCK TIDFYANKD 
      NQFGNGHRF PKNIDKECF NKIQDHYIK CTKEKLNQS 
      EIMNKLNQL SDALNELKK KCRCFEDET QKPQRKPRA 
      VIN
    CDS sequence
    • >TC013247 RA
      atgagttat tctgttgta ccaatagaa gaggaaaag 
      cttcaagag aatgaggaa aacgtcgca aaaagcctc 
      tatcggtgg attcaagga tttagcaaa gcgtgcgac 
      aataacagt atcaagaac attgcaagg cttcaagac 
      aacaaagtc ctcaaaaaa ttcatgcaa ttagacaca 
      aactgtgat ttttcaaca accgaaaag agaaataaa 
      ttcaaatac gaacacata gaaactgta ctaaataaa 
      tatgacatg gataataag aacgctaag gatgtaacg 
      tattctatg ttcttcgca tcagcccta cttgatttg 
      gataaattg gctaaaatc attaaactg aagcctagt 
      ttaattgac caaacaata tattcgaac tccgtgctt 
      atttttact ctcaagtac ggaaacttc tacaaggag 
      aacttcgta gattgtgtg aaagaatta atcgagtct 
      ggtgtcaac gttaacaaa tgtgatctt gtcgggaaa 
      agtgcgatt gattacgtt gctgaatta tacgaagaa 
      aataaagac aacagtgat gtagcagca aagttgaag 
      actgtggcc cgactaatt ttaaataaa actgtatac 
      gggaatgac aataacatg accaagaat ttcctcgaa 
      aaaatcatg aactttgat ttaaacact cttgcggag 
      atggacgtt aaaacaaag ttattttac tgcatagta 
      aagaacaaa ataacggaa cttcagaaa ttggaggat 
      gttgaacaa tacctaaat tgtgacacc gaagaaaac 
      actttactg caaatagct tgcaaacgc aattgcact 
      ttggatgtc atccgctgg ttgctaaat aatggtgct 
      gatgtgaat atgaagacg ccgctaaat cccctcacg 
      ccgttggaa ttagcagca acgcacaac agtattaag 
      atcttcgaa gaaatacta aagtgtaga gaaactaag 
      atcggaaag gaccatttc aacaatctc atcaagtgg 
      cataaaggc tccatgaaa tcaagaata tttgagctt 
      tttttggaa tcagacaag ataaatgtt gaattcaaa 
      tctgaaggg actgggaat acacccctc cattacgcc 
      gtttggttc tcctataaa acagcgatt caaaattta 
      ctacagtgt ggagcgtct ttgactgta aagaacaat 
      aatcaaaag tgccccctt gatataatc gattctgca 
      gacctcgag aaatatttc gacaattgc atagctttg 
      gacaactac aaccagcac tttgcaaac cccgattat 
      aagatgaac attcgtttc acgtcttta atcaaagac 
      ggcgagttg aaagaaagc acagttttt tcgaaaatt 
      gggaatcga ccgaaatta gaacacctt ctgacgcac 
      cccctaatc catttattt attttactg aaatggcac 
      ctaattcgc gtttatttt tggaccgtt ttgctagtt 
      aaagcgaca ttgtattcc ctgttggct tattgcctt 
      tatcatctc cctggttta tcagcaggc ggatatgcg 
      ttttgtgta acactggcg gtaatcaat acttttata 
      aaaataccg attttcctg tactggcaa aaaaagggc 
      aaaagaaat gaatttctc gtaatgcag cgcgcccct 
      ctgcacgct tttctagaa atattttta gtaataata 
      atgtggggc agctttttc cagaaccaa ttccgggcc 
      ttcgttttc atcgcactt gccgtttcg tttctctta 
      accacaggt taccacccc aaactgtcg aaatggtcg 
      atcatgttg cagaaagtt ttcaaaact ttcaccatt 
      ttgttgatt tttttctcg ctcataatt gtggcgttc 
      agtgctggt tttcagatg ctgttcccc ggggaggga 
      cagtttcgg gattttttt agcgccgtt ttcaaaacg 
      tacattatg gccacggac gactacaat attgacgaa 
      cacaccgat tttgactcg gcgggaatt gcgggatat 
      tttatgttc ctgcttttt gcgatgagc atttcgctg 
      gttattgtc aatttctgg accggtgtt gcagtgact 
      gacatcgaa cgaattgaa agaacgtcg gtggtgaac 
      gcgtttagg aacgtcatt attttcatg tctttcatt 
      gagtgggtt tataggttt gaatttctg ggaaaagtt 
      cggggctgt gtaccgaac cctttactg tttcctggt 
      gattgcaaa accattgac ttttatgct aataaagac 
      aaccagttt ggaaatggt cacaggttt ccaaaaaac 
      atcgacaaa gaatgcttc aataaaata caagatcat 
      tacataaaa tgtaccaaa gaaaaactt aaccaaagt 
      gaaatcatg aacaaattg aaccagctt tcggatgcg 
      ctgaatgaa ttaaaaaag aagtgtagg tgttttgag 
      gacgaaacg caaaaacct caaagaaaa cctcgggca 
      gtaattaac tga
    mRNA sequence
    • >TC013247 RA
      catttaatt atttcgcgg gtcttacta tttttcaaa 
      taaaaattt gttaattgt cggctaaag tataaataa 
      aatgagtta ttctgttgt accaataga agaggaaaa 
      gcttcaaga gaatgagga aaacgtcgc aaaaagcct 
      ctatcggtg gattcaagg atttagcaa agcgtgcga 
      caataacag tatcaagaa cattgcaag gcttcaaga 
      caacaaagt cctcaaaaa attcatgca attagacac 
      aaactgtga tttttcaac aaccgaaaa gagaaataa 
      attcaaata cgaacacat agaaactgt actaaataa 
      atatgacat ggataataa gaacgctaa ggatgtaac 
      gtattctat gttcttcgc atcagccct acttgattt 
      ggataaatt ggctaaaat cattaaact gaagcctag 
      tttaattga ccaaacaat atattcgaa ctccgtgct 
      tatttttac tctcaagta cggaaactt ctacaagga 
      gaacttcgt agattgtgt gaaagaatt aatcgagtc 
      tggtgtcaa cgttaacaa atgtgatct tgtcgggaa 
      aagtgcgat tgattacgt tgctgaatt atacgaaga 
      aaataaaga caacagtga tgtagcagc aaagttgaa 
      gactgtggc ccgactaat tttaaataa aactgtata 
      cgggaatga caataacat gaccaagaa tttcctcga 
      aaaaatcat gaactttga tttaaacac tcttgcgga 
      gatggacgt taaaacaaa gttatttta ctgcatagt 
      aaagaacaa aataacgga acttcagaa attggagga 
      tgttgaaca atacctaaa ttgtgacac cgaagaaaa 
      cactttact gcaaatagc ttgcaaacg caattgcac 
      tttggatgt catccgctg gttgctaaa taatggtgc 
      tgatgtgaa tatgaagac gccgctaaa tcccctcac 
      gccgttgga attagcagc aacgcacaa cagtattaa 
      gatcttcga agaaatact aaagtgtag agaaactaa 
      gatcggaaa ggaccattt caacaatct catcaagtg 
      gcataaagg ctccatgaa atcaagaat atttgagct 
      ttttttgga atcagacaa gataaatgt tgaattcaa 
      atctgaagg gactgggaa tacacccct ccattacgc 
      cgtttggtt ctcctataa aacagcgat tcaaaattt 
      actacagtg tggagcgtc tttgactgt aaagaacaa 
      taatcaaaa gtgccccct tgatataat cgattctgc 
      agacctcga gaaatattt cgacaattg catagcttt 
      ggacaacta caaccagca ctttgcaaa ccccgatta 
      taagatgaa cattcgttt cacgtcttt aatcaaaga 
      cggcgagtt gaaagaaag cacagtttt ttcgaaaat 
      tgggaatcg accgaaatt agaacacct tctgacgca 
      ccccctaat ccatttatt tattttact gaaatggca 
      cctaattcg cgtttattt ttggaccgt tttgctagt 
      taaagcgac attgtattc cctgttggc ttattgcct 
      ttatcatct ccctggttt atcagcagg cggatatgc 
      gttttgtgt aacactggc ggtaatcaa tacttttat 
      aaaaatacc gattttcct gtactggca aaaaaaggg 
      caaaagaaa tgaatttct cgtaatgca gcgcgcccc 
      tctgcacgc ttttctaga aatattttt agtaataat 
      aatgtgggg cagcttttt ccagaacca attccgggc 
      cttcgtttt catcgcact tgccgtttc gtttctctt 
      aaccacagg ttaccaccc caaactgtc gaaatggtc 
      gatcatgtt gcagaaagt tttcaaaac tttcaccat 
      tttgttgat ttttttctc gctcataat tgtggcgtt 
      cagtgctgg ttttcagat gctgttccc cggggaggg 
      acagtttcg ggatttttt tagcgccgt tttcaaaac 
      gtacattat ggccacgga cgactacaa tattgacga 
      acacaccga ttttgactc ggcgggaat tgcgggata 
      ttttatgtt cctgctttt tgcgatgag catttcgct 
      ggttattgt caatttctg gaccggtgt tgcagtgac 
      tgacatcga acgaattga aagaacgtc ggtggtgaa 
      cgcgtttag gaacgtcat tattttcat gtctttcat 
      tgagtgggt ttataggtt tgaatttct gggaaaagt 
      tcggggctg tgtaccgaa ccctttact gtttcctgg 
      tgattgcaa aaccattga cttttatgc taataaaga 
      caaccagtt tggaaatgg tcacaggtt tccaaaaaa 
      catcgacaa agaatgctt caataaaat acaagatca 
      ttacataaa atgtaccaa agaaaaact taaccaaag 
      tgaaatcat gaacaaatt gaaccagct ttcggatgc 
      gctgaatga attaaaaaa gaagtgtag gtgttttga 
      ggacgaaac gcaaaaacc tcaaagaaa acctcgggc 
      agtaattaa ctgagaggt ggttgttat aacaaacca 
      ttaaaacac atacaaact ctttgtttc aatttcatt 
      tttaattgg acatttttt gcgtgatct tagctcaaa 
      ataatgtag ttctggttg taatttgat ggttatagg 
      aacatatgc tgtctgttg tttattaag atattaaat 
      atttagtaa tgtttatta gtgtaatgt cggactaaa 
      aaaacaaaa aaattgatt cgtaaaatt tggattaac 
      tcttttaca cctctagtt ttgaacttt tctataata 
      ataatgaat aattttgtt gtacgtatt tgtatgatt 
      gcttgtaag gagtaaaga agaaaaaag aacaaaaga 
      attatttag tattccctt cttcggctc aagatcgaa 
      attggcaga cagtgaagg gaagcgcga tgtgtgcat 
      gtttctcgt tgatgaaaa agtgggcaa acctataat 
      ctaggtatc tatcaaatt aaaggtaga ttagctgca 
      ttgtctgac attgtaaca acagatcca cccggttta 
      gacgaggat atttacatt cgagacgga tttgcaaat 
      atttgagcc tgttgactg ttacctgtt gtacgatct 
      aatcaacaa ccggaacgt ttagcgtcc tctccatcc 
      caatgcacg aaatagaga gagaagaat acggcgtca 
      cgacgccaa taatgcgat tatttcgta ttagttcac 
      tctttattg ccgcgattc cacggcaaa catcaaata 
      tgtgcttaa tacacatgc gtgcctgct acgcctttt 
      aaatcctgc tttagacgt ctgatgaat tagctcagt 
      cgagagaca attatagtt aatttatat gaaaaattt 
      gcagagagt gcacctcca aataacgct tgaatattt 
      cacgagata aaaaacctt atgattttt aataactcg 
      catattgac acaagtgga tgaatattc catttagag 
      atacataat taattttgt tgtgcaagc cgttcttat 
      aagtttcca ttttaaagt tggtctcat ccgagtgga 
      ggcaaaggg ttgagatga cgctgaaca aacaacaag 
      acgaatctg aagcgcttc cttaattaa taaacttct 
      actaatgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY