Status
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Gene information
General information
TC013247 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11558619..11562349
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013247
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05405

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment:
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013247 RA
      MSYSVVPIE EEKLQENEE NVAKSLYRW IQGFSKACD 
NNSIKNIAR LQDNKVLKK FMQLDTNCD FSTTEKRNK 
FKYEHIETV LNKYDMDNK NAKDVTYSM FFASALLDL 
DKLAKIIKL KPSLIDQTI YSNSVLIFT LKYGNFYKE 
NFVDCVKEL IESGVNVNK CDLVGKSAI DYVAELYEE 
NKDNSDVAA KLKTVARLI LNKTVYGND NNMTKNFLE 
KIMNFDLNT LAEMDVKTK LFYCIVKNK ITELQKLED 
VEQYLNCDT EENTLLQIA CKRNCTLDV IRWLLNNGA 
DVNMKTPLN PLTPLELAA THNSIKIFE EILKCRETK 
IGKDHFNNL IKWHKGSMK SRIFELFLE SDKINVEFK 
SEGTGNTPL HYAVWFSYK TAIQNLLQC GASLTVKNN 
NQKCPLDII DSADLEKYF DNCIALDNY NQHFANPDY 
KMNIRFTSL IKDGELKES TVFSKIGNR PKLEHLLTH 
PLIHLFILL KWHLIRVYF WTVLLVKAT LYSLLAYCL 
YHLPGLSAG GYAFCVTLA VINTFIKIP IFLYWQKKG 
KRNEFLVMQ RAPLHAFLE IFLVIIMWG SFFQNQFRA 
FVFIALAVS FLLTTGYHP KLSKWSIML QKVFKTFTI 
LLIFFSLII VAFSAGFQM LFPGEGQFR DFFSAVFKT 
YIMATDDYN IDEHTDFDS AGIAGYFMF LLFAMSISL 
VIVNFWTGV AVTDIERIE RTSVVNAFR NVIIFMSFI 
EWVYRFEFL GKVRGCVPN PLLFPGDCK TIDFYANKD 
NQFGNGHRF PKNIDKECF NKIQDHYIK CTKEKLNQS 
EIMNKLNQL SDALNELKK KCRCFEDET QKPQRKPRA 
VIN
    CDS sequence
    • >TC013247 RA
      atgagttat tctgttgta ccaatagaa gaggaaaag 
cttcaagag aatgaggaa aacgtcgca aaaagcctc 
tatcggtgg attcaagga tttagcaaa gcgtgcgac 
aataacagt atcaagaac attgcaagg cttcaagac 
aacaaagtc ctcaaaaaa ttcatgcaa ttagacaca 
aactgtgat ttttcaaca accgaaaag agaaataaa 
ttcaaatac gaacacata gaaactgta ctaaataaa 
tatgacatg gataataag aacgctaag gatgtaacg 
tattctatg ttcttcgca tcagcccta cttgatttg 
gataaattg gctaaaatc attaaactg aagcctagt 
ttaattgac caaacaata tattcgaac tccgtgctt 
atttttact ctcaagtac ggaaacttc tacaaggag 
aacttcgta gattgtgtg aaagaatta atcgagtct 
ggtgtcaac gttaacaaa tgtgatctt gtcgggaaa 
agtgcgatt gattacgtt gctgaatta tacgaagaa 
aataaagac aacagtgat gtagcagca aagttgaag 
actgtggcc cgactaatt ttaaataaa actgtatac 
gggaatgac aataacatg accaagaat ttcctcgaa 
aaaatcatg aactttgat ttaaacact cttgcggag 
atggacgtt aaaacaaag ttattttac tgcatagta 
aagaacaaa ataacggaa cttcagaaa ttggaggat 
gttgaacaa tacctaaat tgtgacacc gaagaaaac 
actttactg caaatagct tgcaaacgc aattgcact 
ttggatgtc atccgctgg ttgctaaat aatggtgct 
gatgtgaat atgaagacg ccgctaaat cccctcacg 
ccgttggaa ttagcagca acgcacaac agtattaag 
atcttcgaa gaaatacta aagtgtaga gaaactaag 
atcggaaag gaccatttc aacaatctc atcaagtgg 
cataaaggc tccatgaaa tcaagaata tttgagctt 
tttttggaa tcagacaag ataaatgtt gaattcaaa 
tctgaaggg actgggaat acacccctc cattacgcc 
gtttggttc tcctataaa acagcgatt caaaattta 
ctacagtgt ggagcgtct ttgactgta aagaacaat 
aatcaaaag tgccccctt gatataatc gattctgca 
gacctcgag aaatatttc gacaattgc atagctttg 
gacaactac aaccagcac tttgcaaac cccgattat 
aagatgaac attcgtttc acgtcttta atcaaagac 
ggcgagttg aaagaaagc acagttttt tcgaaaatt 
gggaatcga ccgaaatta gaacacctt ctgacgcac 
cccctaatc catttattt attttactg aaatggcac 
ctaattcgc gtttatttt tggaccgtt ttgctagtt 
aaagcgaca ttgtattcc ctgttggct tattgcctt 
tatcatctc cctggttta tcagcaggc ggatatgcg 
ttttgtgta acactggcg gtaatcaat acttttata 
aaaataccg attttcctg tactggcaa aaaaagggc 
aaaagaaat gaatttctc gtaatgcag cgcgcccct 
ctgcacgct tttctagaa atattttta gtaataata 
atgtggggc agctttttc cagaaccaa ttccgggcc 
ttcgttttc atcgcactt gccgtttcg tttctctta 
accacaggt taccacccc aaactgtcg aaatggtcg 
atcatgttg cagaaagtt ttcaaaact ttcaccatt 
ttgttgatt tttttctcg ctcataatt gtggcgttc 
agtgctggt tttcagatg ctgttcccc ggggaggga 
cagtttcgg gattttttt agcgccgtt ttcaaaacg 
tacattatg gccacggac gactacaat attgacgaa 
cacaccgat tttgactcg gcgggaatt gcgggatat 
tttatgttc ctgcttttt gcgatgagc atttcgctg 
gttattgtc aatttctgg accggtgtt gcagtgact 
gacatcgaa cgaattgaa agaacgtcg gtggtgaac 
gcgtttagg aacgtcatt attttcatg tctttcatt 
gagtgggtt tataggttt gaatttctg ggaaaagtt 
cggggctgt gtaccgaac cctttactg tttcctggt 
gattgcaaa accattgac ttttatgct aataaagac 
aaccagttt ggaaatggt cacaggttt ccaaaaaac 
atcgacaaa gaatgcttc aataaaata caagatcat 
tacataaaa tgtaccaaa gaaaaactt aaccaaagt 
gaaatcatg aacaaattg aaccagctt tcggatgcg 
ctgaatgaa ttaaaaaag aagtgtagg tgttttgag 
gacgaaacg caaaaacct caaagaaaa cctcgggca 
gtaattaac tga
    mRNA sequence
    • >TC013247 RA
      catttaatt atttcgcgg gtcttacta tttttcaaa 
taaaaattt gttaattgt cggctaaag tataaataa 
aatgagtta ttctgttgt accaataga agaggaaaa 
gcttcaaga gaatgagga aaacgtcgc aaaaagcct 
ctatcggtg gattcaagg atttagcaa agcgtgcga 
caataacag tatcaagaa cattgcaag gcttcaaga 
caacaaagt cctcaaaaa attcatgca attagacac 
aaactgtga tttttcaac aaccgaaaa gagaaataa 
attcaaata cgaacacat agaaactgt actaaataa 
atatgacat ggataataa gaacgctaa ggatgtaac 
gtattctat gttcttcgc atcagccct acttgattt 
ggataaatt ggctaaaat cattaaact gaagcctag 
tttaattga ccaaacaat atattcgaa ctccgtgct 
tatttttac tctcaagta cggaaactt ctacaagga 
gaacttcgt agattgtgt gaaagaatt aatcgagtc 
tggtgtcaa cgttaacaa atgtgatct tgtcgggaa 
aagtgcgat tgattacgt tgctgaatt atacgaaga 
aaataaaga caacagtga tgtagcagc aaagttgaa 
gactgtggc ccgactaat tttaaataa aactgtata 
cgggaatga caataacat gaccaagaa tttcctcga 
aaaaatcat gaactttga tttaaacac tcttgcgga 
gatggacgt taaaacaaa gttatttta ctgcatagt 
aaagaacaa aataacgga acttcagaa attggagga 
tgttgaaca atacctaaa ttgtgacac cgaagaaaa 
cactttact gcaaatagc ttgcaaacg caattgcac 
tttggatgt catccgctg gttgctaaa taatggtgc 
tgatgtgaa tatgaagac gccgctaaa tcccctcac 
gccgttgga attagcagc aacgcacaa cagtattaa 
gatcttcga agaaatact aaagtgtag agaaactaa 
gatcggaaa ggaccattt caacaatct catcaagtg 
gcataaagg ctccatgaa atcaagaat atttgagct 
ttttttgga atcagacaa gataaatgt tgaattcaa 
atctgaagg gactgggaa tacacccct ccattacgc 
cgtttggtt ctcctataa aacagcgat tcaaaattt 
actacagtg tggagcgtc tttgactgt aaagaacaa 
taatcaaaa gtgccccct tgatataat cgattctgc 
agacctcga gaaatattt cgacaattg catagcttt 
ggacaacta caaccagca ctttgcaaa ccccgatta 
taagatgaa cattcgttt cacgtcttt aatcaaaga 
cggcgagtt gaaagaaag cacagtttt ttcgaaaat 
tgggaatcg accgaaatt agaacacct tctgacgca 
ccccctaat ccatttatt tattttact gaaatggca 
cctaattcg cgtttattt ttggaccgt tttgctagt 
taaagcgac attgtattc cctgttggc ttattgcct 
ttatcatct ccctggttt atcagcagg cggatatgc 
gttttgtgt aacactggc ggtaatcaa tacttttat 
aaaaatacc gattttcct gtactggca aaaaaaggg 
caaaagaaa tgaatttct cgtaatgca gcgcgcccc 
tctgcacgc ttttctaga aatattttt agtaataat 
aatgtgggg cagcttttt ccagaacca attccgggc 
cttcgtttt catcgcact tgccgtttc gtttctctt 
aaccacagg ttaccaccc caaactgtc gaaatggtc 
gatcatgtt gcagaaagt tttcaaaac tttcaccat 
tttgttgat ttttttctc gctcataat tgtggcgtt 
cagtgctgg ttttcagat gctgttccc cggggaggg 
acagtttcg ggatttttt tagcgccgt tttcaaaac 
gtacattat ggccacgga cgactacaa tattgacga 
acacaccga ttttgactc ggcgggaat tgcgggata 
ttttatgtt cctgctttt tgcgatgag catttcgct 
ggttattgt caatttctg gaccggtgt tgcagtgac 
tgacatcga acgaattga aagaacgtc ggtggtgaa 
cgcgtttag gaacgtcat tattttcat gtctttcat 
tgagtgggt ttataggtt tgaatttct gggaaaagt 
tcggggctg tgtaccgaa ccctttact gtttcctgg 
tgattgcaa aaccattga cttttatgc taataaaga 
caaccagtt tggaaatgg tcacaggtt tccaaaaaa 
catcgacaa agaatgctt caataaaat acaagatca 
ttacataaa atgtaccaa agaaaaact taaccaaag 
tgaaatcat gaacaaatt gaaccagct ttcggatgc 
gctgaatga attaaaaaa gaagtgtag gtgttttga 
ggacgaaac gcaaaaacc tcaaagaaa acctcgggc 
agtaattaa ctgagaggt ggttgttat aacaaacca 
ttaaaacac atacaaact ctttgtttc aatttcatt 
tttaattgg acatttttt gcgtgatct tagctcaaa 
ataatgtag ttctggttg taatttgat ggttatagg 
aacatatgc tgtctgttg tttattaag atattaaat 
atttagtaa tgtttatta gtgtaatgt cggactaaa 
aaaacaaaa aaattgatt cgtaaaatt tggattaac 
tcttttaca cctctagtt ttgaacttt tctataata 
ataatgaat aattttgtt gtacgtatt tgtatgatt 
gcttgtaag gagtaaaga agaaaaaag aacaaaaga 
attatttag tattccctt cttcggctc aagatcgaa 
attggcaga cagtgaagg gaagcgcga tgtgtgcat 
gtttctcgt tgatgaaaa agtgggcaa acctataat 
ctaggtatc tatcaaatt aaaggtaga ttagctgca 
ttgtctgac attgtaaca acagatcca cccggttta 
gacgaggat atttacatt cgagacgga tttgcaaat 
atttgagcc tgttgactg ttacctgtt gtacgatct 
aatcaacaa ccggaacgt ttagcgtcc tctccatcc 
caatgcacg aaatagaga gagaagaat acggcgtca 
cgacgccaa taatgcgat tatttcgta ttagttcac 
tctttattg ccgcgattc cacggcaaa catcaaata 
tgtgcttaa tacacatgc gtgcctgct acgcctttt 
aaatcctgc tttagacgt ctgatgaat tagctcagt 
cgagagaca attatagtt aatttatat gaaaaattt 
gcagagagt gcacctcca aataacgct tgaatattt 
cacgagata aaaaacctt atgattttt aataactcg 
catattgac acaagtgga tgaatattc catttagag 
atacataat taattttgt tgtgcaagc cgttcttat 
aagtttcca ttttaaagt tggtctcat ccgagtgga 
ggcaaaggg ttgagatga cgctgaaca aacaacaag 
acgaatctg aagcgcttc cttaattaa taaacttct 
actaatgaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY