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Gene information
General information
TC013191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12557204..12558535
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013191
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05400

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013191 RA
      MGIDFYGVL QIPRSSTNL DIKKAFRDL ALEFNPEKL 
      QGENAQQVF SLICEAYDV LSDPLRRSV FDQYGEEGL 
      KRGVPGPDS FIEPYRYHG DPMRTYKDF FGTTSPYAN 
      LLDYLRNPS YECMTKHGK IFCEKQPPI THPLHLTLH 
      EIFFGGIKK MKIHRLVYI NDEKTKTKV KEKILTIPI 
      KPGVRPGTE LVFPEEGDQ SSNHVPADV IFVVQERPH 
      EVFQREEDN LAMMCSVTL EEALMGTTV TVNTIDHRT 
      VRVPITDVI FPGYEKIVE NEGMPVLDD YPKRGNLII 
      RFDIAFPKY LPKACKHLL RKGFQLAKI GGGVDQYET 
      VNKLVLADK ILRVDLNEQ MPP
    CDS sequence
    • >TC013191 RA
      atggggata gatttttac ggagtgttg caaataccg 
      cggagcagc acgaacctc gacatcaag aaggcgttt 
      cgggacttg gctcttgaa ttcaaccct gagaaactc 
      cagggcgag aatgcccag caagtgttc agcctcatt 
      tgcgaggct tacgacgtt ctcagcgac cctctccgg 
      agatcagtg ttcgatcaa tatggcgaa gagggcttg 
      aagcgggga gtccccggg cctgatagt ttcatagag 
      ccttaccgc taccacggg gaccccatg cgcacttat 
      aaagatttt ttcggtacc acttcgccc tacgccaat 
      ttgctcgat tatttgcgc aacccctcg tacgagtgc 
      atgaccaag cacgggaaa atcttctgt gagaagcaa 
      ccgccgatt acccaccct ctgcacttg accctccac 
      gagattttc ttcgggggg attaaaaaa atgaagatt 
      catcgcctg gtttacatc aacgacgaa aagacgaaa 
      accaaagtt aaggagaag atcctcact attcccatc 
      aagccgggg gttaggcct gggacagag ctggttttc 
      ccggaggaa ggcgaccag agttcgaat catgttccc 
      gctgacgtt attttcgtg gttcaggaa aggccccac 
      gaagtgttc caaagggag gaggataat ttggctatg 
      atgtgtagt gtgacactc gaggaggcg ctaatgggg 
      accacggtc actgttaac acaatcgac catcgcaca 
      gttcgggtg ccaataaca gacgtcatt tttcctggg 
      tacgaaaaa atcgtggag aatgaggga atgccggtt 
      ttggacgat tatcccaaa aggggaaac ctgataata 
      cggttcgac attgccttt ccgaaatat ttaccaaag 
      gcttgtaaa catttgtta cggaaagga ttccagctg 
      gcgaagatt ggcggtggt gttgatcaa tacgaaacg 
      gttaataag ctggttttg gccgataaa attcttcgc 
      gttgatctc aacgagcaa atgccacca taa
    mRNA sequence
    • >TC013191 RA
      acaatcatc tagtagtga gttgtccca acgccaaaa 
      ttttcgacg aaatcgctg tcaaaaaat ggggataga 
      tttttacgg agtgttgca aataccgcg gagcagcac 
      gaacctcga catcaagaa ggcgtttcg ggacttggc 
      tcttgaatt caaccctga gaaactcca gggcgagaa 
      tgcccagca agtgttcag cctcatttg cgaggctta 
      cgacgttct cagcgaccc tctccggag atcagtgtt 
      cgatcaata tggcgaaga gggcttgaa gcggggagt 
      ccccgggcc tgatagttt catagagcc ttaccgcta 
      ccacgggga ccccatgcg cacttataa agatttttt 
      cggtaccac ttcgcccta cgccaattt gctcgatta 
      tttgcgcaa cccctcgta cgagtgcat gaccaagca 
      cgggaaaat cttctgtga gaagcaacc gccgattac 
      ccaccctct gcacttgac cctccacga gattttctt 
      cggggggat taaaaaaat gaagattca tcgcctggt 
      ttacatcaa cgacgaaaa gacgaaaac caaagttaa 
      ggagaagat cctcactat tcccatcaa gccgggggt 
      taggcctgg gacagagct ggttttccc ggaggaagg 
      cgaccagag ttcgaatca tgttcccgc tgacgttat 
      tttcgtggt tcaggaaag gccccacga agtgttcca 
      aagggagga ggataattt ggctatgat gtgtagtgt 
      gacactcga ggaggcgct aatggggac cacggtcac 
      tgttaacac aatcgacca tcgcacagt tcgggtgcc 
      aataacaga cgtcatttt tcctgggta cgaaaaaat 
      cgtggagaa tgagggaat gccggtttt ggacgatta 
      tcccaaaag gggaaacct gataatacg gttcgacat 
      tgcctttcc gaaatattt accaaaggc ttgtaaaca 
      tttgttacg gaaaggatt ccagctggc gaagattgg 
      cggtggtgt tgatcaata cgaaacggt taataagct 
      ggttttggc cgataaaat tcttcgcgt tgatctcaa 
      cgagcaaat gccaccata atgttatct acttacgat 
      ttcaatttt ttttgaaga ataaatatt tttaactgt 
      ttcttttac tttgaatcc acccaaata ctcaataaa 
      gtgttaaga aaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY