Status
Please wait ...
Gene information
General information
TC013190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12561526..12568022
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013190
    iBeetle screen iB_05399

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013190 RA
      MVDAPGKKK GFWISYPPP GAGSSSSSK DKEKQQQTE 
      EDKRPATRE QRQLEYDAA IKEHHRSQR EQWEIRQLD 
      NDLLQQRHS EREREREEK ELAEQQHHH LQQLLLVDD 
      KHDFTSHSC RAPIASLDC MEEFSAGSA LSALTDRSG 
      DGGITKEQL LLSGLTQSA PNAALQLAK QTSTAPGLR 
      YRNLGKSGL RVSNIGLGT WPTFGPNVT EEQAEQIIV 
      LAVESGINV FDLSEAHSG TRAEVELGR ILARRGWKR 
      TCYIVTTKI YWSTRSEER GLSRKHIIE SVKASLVRL 
      QLSYIDVVI VHKADPMCP MEEVVRAMH YVISQGWVM 
      YWGTARWSP VEVMEAYTN CRQFNCVTP IVEQAEYHM 
      FCREKTELY MPELYNKIG VGLMAWSPI SMGLTQGKE 
      DASVQLFSR ASFRNKYSS FSWTEDETT ANKDGYNWM 
      KERIQPEET RRQSERIRE LNTVAEKLG CTLSQLAIA 
      WCLKNESVQ CLLLGASTV VQLYDSIQS LQLVPKLNT 
      TMIAEIERI LENKPSRPP MVSTLALR
    CDS sequence
    • >TC013190 RA
      atggtggat gcgccgggc aagaaaaaa gggttttgg 
      atatcatac ccgccgccg ggggcgggc agctcctcc 
      agttccaaa gacaaagaa aagcagcaa caaaccgaa 
      gaagacaag cgtccagca acacgcgaa cagcgccaa 
      ttggaatac gacgctgca attaaagaa catcaccgt 
      tctcagaga gaacagtgg gagattaga caattagat 
      aacgacttg ctgcagcaa cgacactct gaacgcgaa 
      cgcgaacgc gaggaaaag gagttggcc gaacagcaa 
      caccaccac ctccaacaa ctccttctc gtggacgac 
      aaacatgac tttacttca cactcgtgt agggcacca 
      atagcaagt ttagactgt atggaagaa tttagcgct 
      ggaagcgca ctcagtgca ctcaccgat cgctcaggt 
      gacggcgga atcacaaaa gagcaactt ttactgagc 
      ggtttgact cagtcagca ccaaatgca gctttacag 
      cttgccaaa caaacgtca acggccccc ggcctgaga 
      taccgcaat ctgggaaaa agcggctta agagtgtcc 
      aacattgga ctaggcact tggcccaca ttcggacca 
      aatgtcacc gaagagcaa gccgaacag atcatcgtc 
      ctagctgtg gaaagcgga atcaacgtg ttcgacctc 
      tcggaagcc cactcagga actagagcc gaggtggaa 
      ctgggcagg attctggca cggcgcggg tggaagcgg 
      acgtgttac attgtaacc acgaaaata tactggagc 
      accaggtcg gaagaacga ggtttatcc cggaaacat 
      ataatcgaa agtgtgaaa gctagtctt gtccgtttg 
      caactttct tatattgat gttgttatc gtccacaag 
      gcggatccc atgtgtcca atggaagaa gtagtaaga 
      gcgatgcac tatgtcatt tcacaaggt tgggtcatg 
      tactggggc acagccagg tggtccccc gttgaggtc 
      atggaagct tacaccaac tgtcggcag tttaactgc 
      gtcaccccc attgtggag caagccgag taccacatg 
      ttctgtcgg gaaaaaacc gaactctac atgccggag 
      ttgtacaac aagattgga gttggtttg atggcttgg 
      tcgcccatt tccatgggc ttgacgcag ggcaaagag 
      gacgccagt gtgcagctg ttctcccgg gcgagtttc 
      cggaacaag tacagttcg ttttcctgg accgaggac 
      gaaacgact gccaataaa gacggctac aactggatg 
      aaggaacgc atccagcct gaggaaacc cgccgccag 
      tccgaacga atccgcgag ctgaacaca gtggcagaa 
      aaactaggc tgtacctta tcccaacta gcaatagcg 
      tggtgtctg aagaacgaa tcggtgcaa tgtcttcta 
      cttggagcc agcacggtc gtgcaactc tacgacagt 
      atacagtct cttcaattg gtccctaaa ctgaacacc 
      accatgata gcagaaata gagcgaatc ctagaaaat 
      aaaccgtct agacctccc atggtgtcc actctggcg 
      cttagatga
    mRNA sequence
    • >TC013190 RA
      atggtggat gcgccgggc aagaaaaaa gggttttgg 
      atatcatac ccgccgccg ggggcgggc agctcctcc 
      agttccaaa gacaaagaa aagcagcaa caaaccgaa 
      gaagacaag cgtccagca acacgcgaa cagcgccaa 
      ttggaatac gacgctgca attaaagaa catcaccgt 
      tctcagaga gaacagtgg gagattaga caattagat 
      aacgacttg ctgcagcaa cgacactct gaacgcgaa 
      cgcgaacgc gaggaaaag gagttggcc gaacagcaa 
      caccaccac ctccaacaa ctccttctc gtggacgac 
      aaacatgac tttacttca cactcgtgt agggcacca 
      atagcaagt ttagactgt atggaagaa tttagcgct 
      ggaagcgca ctcagtgca ctcaccgat cgctcaggt 
      gacggcgga atcacaaaa gagcaactt ttactgagc 
      ggtttgact cagtcagca ccaaatgca gctttacag 
      cttgccaaa caaacgtca acggccccc ggcctgaga 
      taccgcaat ctgggaaaa agcggctta agagtgtcc 
      aacattgga ctaggcact tggcccaca ttcggacca 
      aatgtcacc gaagagcaa gccgaacag atcatcgtc 
      ctagctgtg gaaagcgga atcaacgtg ttcgacctc 
      tcggaagcc cactcagga actagagcc gaggtggaa 
      ctgggcagg attctggca cggcgcggg tggaagcgg 
      acgtgttac attgtaacc acgaaaata tactggagc 
      accaggtcg gaagaacga ggtttatcc cggaaacat 
      ataatcgaa agtgtgaaa gctagtctt gtccgtttg 
      caactttct tatattgat gttgttatc gtccacaag 
      gcggatccc atgtgtcca atggaagaa gtagtaaga 
      gcgatgcac tatgtcatt tcacaaggt tgggtcatg 
      tactggggc acagccagg tggtccccc gttgaggtc 
      atggaagct tacaccaac tgtcggcag tttaactgc 
      gtcaccccc attgtggag caagccgag taccacatg 
      ttctgtcgg gaaaaaacc gaactctac atgccggag 
      ttgtacaac aagattgga gttggtttg atggcttgg 
      tcgcccatt tccatgggc ttgacgcag ggcaaagag 
      gacgccagt gtgcagctg ttctcccgg gcgagtttc 
      cggaacaag tacagttcg ttttcctgg accgaggac 
      gaaacgact gccaataaa gacggctac aactggatg 
      aaggaacgc atccagcct gaggaaacc cgccgccag 
      tccgaacga atccgcgag ctgaacaca gtggcagaa 
      aaactaggc tgtacctta tcccaacta gcaatagcg 
      tggtgtctg aagaacgaa tcggtgcaa tgtcttcta 
      cttggagcc agcacggtc gtgcaactc tacgacagt 
      atacagtct cttcaattg gtccctaaa ctgaacacc 
      accatgata gcagaaata gagcgaatc ctagaaaat 
      aaaccgtct agacctccc atggtgtcc actctggcg 
      cttagatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY