Status
Please wait ...
Gene information
General information
TC013185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12620444..12623685
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013185
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05398

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    mature egg deposition 5 number 2nd lateral oviduct: 3 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Comment: The number of eggs on day 13 is not much less compared to the other wells.
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013185 RA
      MLDNQLAEK QSIIFQGKA EKWEVDSST TAKNCKNYI 
      REIVDTLDL QPNPEKPVI ALSIGDPTV YGNLKPSEE 
      TTQAVIDVI REGSCNGYA PCVGYDKSR EAVANYLNQ 
      NGSKLTKND IILCSGCSS SLEICITAL CDAKKNHNL 
      LMPRPGFSI YRTLAEAIG VTVRYYNLI PEKNWQIDV 
      DHLRSQIDQ NTAVIVLNN PSNPCGSVY SAEHLKDVL 
      EVAFTHRIP VIADEIYER LVFPGNSFH STAVLNSGV 
      PLLICGGLA KRFLAPGWR LGWIAICDE GGAFEPNVR 
      KALISLSQR IIGSNTLVQ GALPAILFK TPQSFHDNL 
      INTLHKNAI LAYSMLQNI EGLVPYMPQ GTMYMMVEL 
      CFNRFPFSD VLQFVIKMM EEESVFCLP GDCFQISGF 
      IRIVLTVPE DLLLEACKR ISEFCKRHI IK
    CDS sequence
    • >TC013185 RA
      atgttagat aaccaactg gcggagaaa caatcgatc 
      atttttcaa ggaaaagcc gaaaagtgg gaagtggac 
      tcttcgaca acagccaaa aattgcaaa aactacata 
      agggagatc gtagacacc ctagatctc cagcccaac 
      cccgaaaag cccgtgatc gcgctctcc ataggtgac 
      cctacggtt tacggcaac ttgaaaccc tctgaagaa 
      accactcaa gccgtcatc gacgtcatc cgcgagggc 
      tcttgcaac ggctacgcc ccttgcgtc ggctacgac 
      aaatcccgc gaagccgtc gcaaactac ttgaaccaa 
      aacggctca aaactgacc aaaaacgac ataattctc 
      tgcagcggc tgctcctca tcgctcgaa atctgcata 
      accgccctt tgcgatgcc aaaaagaac cacaacctg 
      ctcatgccc cggcccggc ttctccatc taccgcacc 
      ctagccgag gccatcgga gtcaccgta cgctactac 
      aacttaatc cccgaaaaa aactggcaa attgacgtc 
      gaccacttg cgctcgcaa atcgaccag aacaccgca 
      gtcatcgtc ttgaacaac ccgtccaac ccctgcggg 
      tccgtgtac agtgccgag cacctgaag gacgtcctc 
      gaggtggcc ttcacgcac cgaatcccc gtcatcgcc 
      gacgagatc tacgaacgc ctggtgttt cccgggaac 
      tcgttccac tcgacggcg gttttgaac tcgggggtt 
      cccctcttg atttgtggg gggctggcg aagaggttt 
      ttggcgcca gggtggcgg ctaggttgg attgcgatc 
      tgtgatgaa ggtggagct ttcgagccc aatgtgcgc 
      aaggcgctg attagtctg agccagagg attattggg 
      agcaatact ctggttcag ggagcactg cctgccata 
      ttattcaaa acgccccaa agttttcac gataatttg 
      atcaacacg ctgcataag aatgccatt ttggcctac 
      agtatgttg cagaatatt gaggggctt gtgccctac 
      atgccccag gggaccatg tacatgatg gtggagctc 
      tgcttcaac aggtttccc ttcagtgat gttttgcag 
      tttgttatt aagatgatg gaagaggag tcggttttc 
      tgtttgcca ggagactgc tttcagatt tctggattc 
      ataaggatt gtcctgact gtgcctgaa gacttactg 
      ctggaagct tgcaaacgt atttctgag ttttgcaag 
      cgccatatt ataaaataa
    mRNA sequence
    • >TC013185 RA
      ccagatcta agagtgata ctcgtattt tcgcttata 
      aattagcat atttaataa attaatccg gtttgctat 
      tccatatga ataatattt aaatacctg cccacatcg 
      aggtcgttc agatttgta tttttcttg ataaagaat 
      agttagtac caagcagtt tccaaaaaa ttttcaaaa 
      atcttgtgt gacgatgtt agataacca actggcgga 
      gaaacaatc gatcatttt tcaaggaaa agccgaaaa 
      gtgggaagt ggactcttc gacaacagc caaaaattg 
      caaaaacta cataaggga gatcgtaga caccctaga 
      tctccagcc caaccccga aaagcccgt gatcgcgct 
      ctccatagg tgaccctac ggtttacgg caacttgaa 
      accctctga agaaaccac tcaagccgt catcgacgt 
      catccgcga gggctcttg caacggcta cgccccttg 
      cgtcggcta cgacaaatc ccgcgaagc cgtcgcaaa 
      ctacttgaa ccaaaacgg ctcaaaact gaccaaaaa 
      cgacataat tctctgcag cggctgctc ctcatcgct 
      cgaaatctg cataaccgc cctttgcga tgccaaaaa 
      gaaccacaa cctgctcat gccccggcc cggcttctc 
      catctaccg caccctagc cgaggccat cggagtcac 
      cgtacgcta ctacaactt aatccccga aaaaaactg 
      gcaaattga cgtcgacca cttgcgctc gcaaatcga 
      ccagaacac cgcagtcat cgtcttgaa caacccgtc 
      caacccctg cgggtccgt gtacagtgc cgagcacct 
      gaaggacgt cctcgaggt ggccttcac gcaccgaat 
      ccccgtcat cgccgacga gatctacga acgcctggt 
      gtttcccgg gaactcgtt ccactcgac ggcggtttt 
      gaactcggg ggttcccct cttgatttg tggggggct 
      ggcgaagag gtttttggc gccagggtg gcggctagg 
      ttggattgc gatctgtga tgaaggtgg agctttcga 
      gcccaatgt gcgcaaggc gctgattag tctgagcca 
      gaggattat tgggagcaa tactctggt tcagggagc 
      actgcctgc catattatt caaaacgcc ccaaagttt 
      tcacgataa tttgatcaa cacgctgca taagaatgc 
      cattttggc ctacagtat gttgcagaa tattgaggg 
      gcttgtgcc ctacatgcc ccaggggac catgtacat 
      gatggtgga gctctgctt caacaggtt tcccttcag 
      tgatgtttt gcagtttgt tattaagat gatggaaga 
      ggagtcggt tttctgttt gccaggaga ctgctttca 
      gatttctgg attcataag gattgtcct gactgtgcc 
      tgaagactt actgctgga agcttgcaa acgtatttc 
      tgagttttg caagcgcca tattataaa ataagctgc 
      aattttgtt tctattgtt taagcgcta aaaatataa 
      acttgaaat tact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY