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Gene information
General information
TC013156 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13103803..13109734
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013156
    iBeetle screen iB_05391

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013156 RA
      MKTEDKNIP KVRLAVIGK ANVGKSALT 
      VRYLTRRFI GEYRSNTDL LYRQTLTVN 
      NSALEVEIV DVCSCDLSL FPEEAIYWA 
      DACVVVYDI TNRTSMTHA AELLQRVLQ 
      LRSQMPIVL LGNKADLEH LRQVDEVEG 
      RTLAIQHNS QFHEVSVAE NSPAIYTAI 
      DSVLNECRS VQNTQKTRK FSVSKMIGT 
      LIGNANGKV VPNTNQGGT VVVCHKSDL 
      YKSRVLRRR QNFTATASL
    CDS sequence
    • >TC013156 RA
      atgaaaact gaagacaag aacatacca aaggtccgt 
      ttggccgtc attggcaaa gccaacgtc ggaaaatca 
      gcactaacc gttcgatat ctcactaga aggttcatt 
      ggggagtac cggagcaac acagatctg ctctatcgg 
      caaacgctc accgtgaat aactcggcc ttggaagtc 
      gagattgtg gacgtctgc agttgcgat ctgtctcta 
      ttccctgaa gaagccatt tattgggcg gatgcctgt 
      gttgtggtt tacgatata accaacagg acgtcgatg 
      acacatgcc gccgagctg ctccagagg gtgctccag 
      ttgcggagt cagatgcct attgtcctg ctggggaac 
      aaagccgat ctggaacac cttcgacag gtggacgag 
      gtggaaggg cgcaccctg gccatccag cacaactcg 
      cagttccac gaggtctcg gtggccgag aacagtccg 
      gccatctac accgccatc gactccgtg ctgaacgag 
      tgccgttcg gtgcaaaac acgcagaaa acgcgcaaa 
      ttttccgtg tcgaagatg atcgggacg ctgatcggg 
      aacgcaaac ggtaaagtc gtcccgaac acgaaccaa 
      gggggcact gtggtggtg tgccacaag tccgacttg 
      tacaagtcg cgcgtgctg agacggcgg cagaatttt 
      acagcgacg gcatcgctc tga
    mRNA sequence
    • >TC013156 RA
      gtaaacgcg cctctcagc gtcaaacca gcagcaaag 
      tgcgagcgt gtttggttg tgaatagac agttttgag 
      actgaaaat tctcgagtg cgccgttcc ttggggcta 
      cgtctccga cttaatcca gaaaatgaa aactgaaga 
      caagaacat accaaaggt ccgtttggc cgtcattgg 
      caaagccaa cgtcggaaa atcagcact aaccgttcg 
      atatctcac tagaaggtt cattgggga gtaccggag 
      caacacaga tctgctcta tcggcaaac gctcaccgt 
      gaataactc ggccttgga agtcgagat tgtggacgt 
      ctgcagttg cgatctgtc tctattccc tgaagaagc 
      catttattg ggcggatgc ctgtgttgt ggtttacga 
      tataaccaa caggacgtc gatgacaca tgccgccga 
      gctgctcca gagggtgct ccagttgcg gagtcagat 
      gcctattgt cctgctggg gaacaaagc cgatctgga 
      acaccttcg acaggtgga cgaggtgga agggcgcac 
      cctggccat ccagcacaa ctcgcagtt ccacgaggt 
      ctcggtggc cgagaacag tccggccat ctacaccgc 
      catcgactc cgtgctgaa cgagtgccg ttcggtgca 
      aaacacgca gaaaacgcg caaattttc cgtgtcgaa 
      gatgatcgg gacgctgat cgggaacgc aaacggtaa 
      agtcgtccc gaacacgaa ccaaggggg cactgtggt 
      ggtgtgcca caagtccga cttgtacaa gtcgcgcgt 
      gctgagacg gcggcagaa ttttacagc gacggcatc 
      gctctgact gtcggacaa ggaggagca ggtcgggac 
      tgctcgctg aagtgaatt cgttccata tttctcagt 
      tttatgaga gggacgcgt gctgtgcag gactggtcc 
      ttggactaa atacgagta ataagggag ccaagttgc 
      gcggtttgg gaagaatgt gcaataatt catcttggc 
      aggaaggct tccgacagg gtcaatgtt tgtacatat 
      caggacgtc tcaacagct gggctggcc ggactggga 
      gcgcgatta ttttaataa ctaggcgta atttttata 
      ccctttcta tattcatgt tgccaatct taacagaat 
      aatatttag atttgcgaa aaaataccc aactgtcgg 
      ccttgtgat tgcgggagt tgggtgttc gctcgcttg 
      tttcaaaac ccctgatgt ctaggttat aggtgacca 
      aatatttaa taagatggt gtgtgctat gacggctgt 
      gatactaaa tgttttttg taatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY