| General information | |||
| TC031157
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 13230082..13256368 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031157 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05390 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC031157 RA
MRSGPPTLR QHFRLQQHF NAPQQTHTD SNNNSPAYN FHLQNKCTV LVQGEVSSA PLVAASAQP SAPTSPQSS MLLLQTQTP FGSSSFADL LSAPFSDDP SSVLPPEDL DPFPDVALQ SQPASPAPL PSFQETYSV RYNAPFKMD EECFNVSYH HPPHHVSAP HHYDYQQLQ YTASSPYYP PPQTCSPGF DTLISQETY SLPAYQTAT SELHVSTAL SPRQRRASL PLQRSESTS SSESPKLRM PGPSASSSA ASSPGGIAE PPSKGPTPP SPSQLCAVC GDTAACQHY GVRTCEGCK GFFKRTVQK GSKYVCLAD KACPVDKRR RNRCQFCRF QKCLAVGMV KEVVRTDSL KGRRGRLPS KPKSPQESP PSPPVSLIT ALVRAHVDT TPDLANLDY SQYLEPSPI EPVISEAEK IQQFYTLLT TSVDVIKNF ADKIPGFQE LCPEDRELL FQSASLELF VLRLAYRTH PNDTKLTFC NGVVLDKQQ CQRSFGDWL HAIMEFCSS LMAMEIDIS AFACLCALT LITERHGLK EPHRVEQLQ MKIISSLRD HVTYNAEAQ RKAHYFSRL LGKLPELRS LSVQGLQRI FYLKLEDLV PAPPLIEKM FVASLPF
- >TC031157 RA
atgcgctca ggtcctccg actctacgg cagcacttc aggcttcag caacacttt aacgcgccg caacagacg cacactgac tccaacaac aactcgccc gcatataac ttccatctt cagaacaag tgtacagtg ttagtacag ggcgaagtc tcaagtgcc cccctggtg gcggcgagc gcgcagcct tccgcccca actagtccc cagtcaagt atgctcctg ctccagaca cagaccccg tttggctcg agcagtttc gcggatctg ctgagtgcg cccttttcg gacgacccg agcagcgtc ttgccccct gaagacctc gaccccttc ccggacgtc gccctacag tcgcagccg gcctctccg gcgccgctt cccagcttc caggagacc tactcagtc cgttacaac gctccgttc aaaatggac gaagaatgc ttcaacgtc tcctaccac cacccccca caccacgtc tccgcgccg caccattac gactaccaa caacttcaa tacaccgct tccagccct tactacccg cccccgcag acctgcagt cccggcttc gacaccctc atctcccaa gaaacctac tccctccca gcctaccaa accgccacg tcggaactc cacgtcagc accgcttta agcccccgc caacgtcgc gcctctttg cccctccaa cgctccgaa agcaccagc agcagcgag agccccaaa ctccggatg cccggaccc tccgccagc tcctcggcg gcgagttcc cccggaggc atcgccgag ccgccctcc aaaggcccc acacccccg tccccatcc caactctgc gccgtctgc ggcgacacc gccgcctgc cagcactac ggcgtccgc acctgcgag ggctgcaag ggcttcttc aaacgcacc gtccagaaa ggctccaag tacgtgtgt ctcgccgac aaggcctgc cctgtggac aagaggcgg cggaaccgg tgccagttc tgccgcttc cagaagtgt ctcgccgtg gggatggtc aaggaggtc gtccggacg gactccctg aagggcagg agaggcaga ctgccgtcg aaacccaag tctccgcag gaatcacca ccgagtcct cccgtctca ctcatcaca gctttagtt cgcgcacat gtcgacacc acccctgat ctcgctaat ttagactac tcgcaatac ctagagccc agtccgatc gagccggtc atctccgaa gccgagaag atccagcag ttctacacc ctgctgacc acctcggtg gacgtgatc aagaacttc gccgacaag atccccggc ttccaggag ctgtgtccc gaagaccgg gagctcctg ttccagtcc gcctctctg gagctgttc gtcctccgg ctggcttac cgcacgcac ccgaacgac accaaactg acgttctgc aacggcgtc gtgctggac aagcagcag tgtcaaagg tccttcggt gattggttg catgccatc atggagttc tgcagcagc ttgatggcc atggagatt gatatttcg gcgttcgcc tgtctgtgt gcactcacc ctcatcacc gagcggcac ggtctgaag gagccccac cgtgtggag cagctccaa atgaagata atctcctcg ctgagggat catgtgacg tacaacgcg gaggcgcag aggaaggcg cactacttt agccggcta ttgggcaaa ctgccggag ctgaggtcc ttgagcgtg caaggactg cagaggatc ttttacctg aagctggaa gacttggtc ccggcccct cccctcatc gagaagatg ttcgtagct agcctacct ttctaa
- >TC031157 RA
gtgcgtatc tcgcaagaa gttggaccg caagcagca aagtgcaag aagtgcatc gcttcgcgg actgaaata gtattggtt tcagcgcga cgcggtttc agtgagctt tgtggtgga gtgatcttg ccgttatta tgcttgttt tatgaagat actgttcgc agtggattc gcctgaaac gaaggaagg cgcgcgctc ttctaccaa ttcaccgga gtcctcgct ccgggagtc gccaggtga tttgttcga tgttggtag ttgatgctc acttttgtc cgcctgctg tgttttttc gttgtttat gcatgtgga ctagagact agtttagag gattattgc gatgcaacc gttacgaga tgcgctcag gtcctccga ctctacggc agcacttca ggcttcagc aacacttta acgcgccgc aacagacgc acactgact ccaacaaca actcgcccg catataact tccatcttc agaacaagt gtacagtgt tagtacagg gcgaagtct caagtgccc ccctggtgg cggcgagcg cgcagcctt ccgccccaa ctagtcccc agtcaagta tgctcctgc tccagacac agaccccgt ttggctcga gcagtttcg cggatctgc tgagtgcgc ccttttcgg acgacccga gcagcgtct tgccccctg aagacctcg accccttcc cggacgtcg ccctacagt cgcagccgg cctctccgg cgccgcttc ccagcttcc aggagacct actcagtcc gttacaacg ctccgttca aaatggacg aagaatgct tcaacgtct cctaccacc accccccac accacgtct ccgcgccgc accattacg actaccaac aacttcaat acaccgctt ccagccctt actacccgc ccccgcaga cctgcagtc ccggcttcg acaccctca tctcccaag aaacctact ccctcccag cctaccaaa ccgccacgt cggaactcc acgtcagca ccgctttaa gcccccgcc aacgtcgcg cctctttgc ccctccaac gctccgaaa gcaccagca gcagcgaga gccccaaac tccggatgc ccggaccct ccgccagct cctcggcgg cgagttccc ccggaggca tcgccgagc cgccctcca aaggcccca cacccccgt ccccatccc aactctgcg ccgtctgcg gcgacaccg ccgcctgcc agcactacg gcgtccgca cctgcgagg gctgcaagg gcttcttca aacgcaccg tccagaaag gctccaagt acgtgtgtc tcgccgaca aggcctgcc ctgtggaca agaggcggc ggaaccggt gccagttct gccgcttcc agaagtgtc tcgccgtgg ggatggtca aggaggtcg tccggacgg actccctga agggcagga gaggcagac tgccgtcga aacccaagt ctccgcagg aatcaccac cgagtcctc ccgtctcac tcatcacag ctttagttc gcgcacatg tcgacacca cccctgatc tcgctaatt tagactact cgcaatacc tagagccca gtccgatcg agccggtca tctccgaag ccgagaaga tccagcagt tctacaccc tgctgacca cctcggtgg acgtgatca agaacttcg ccgacaaga tccccggct tccaggagc tgtgtcccg aagaccggg agctcctgt tccagtccg cctctctgg agctgttcg tcctccggc tggcttacc gcacgcacc cgaacgaca ccaaactga cgttctgca acggcgtcg tgctggaca agcagcagt gtcaaaggt ccttcggtg attggttgc atgccatca tggagttct gcagcagct tgatggcca tggagattg atatttcgg cgttcgcct gtctgtgtg cactcaccc tcatcaccg agcggcacg gtctgaagg agccccacc gtgtggagc agctccaaa tgaagataa tctcctcgc tgagggatc atgtgacgt acaacgcgg aggcgcaga ggaaggcgc actacttta gccggctat tgggcaaac tgccggagc tgaggtcct tgagcgtgc aaggactgc agaggatct tttacctga agctggaag acttggtcc cggcccctc ccctcatcg agaagatgt tcgtagcta gcctacctt tctaaattg ttagttgat atttttcga aacgtgata aactcttct atgtgattt gttaaatag gtcgtacgt attagtctt tcccgaggg cgagggaac tgcccgaaa atccggtca cggcctaaa aaagacgct tttttggga cgatgatgg tggggctgg tggactttc gggcagttt cgccagatt tcgaattat gttgagaac ttttggtgt tgcagttct tgtgcacga tagacacct tttccactt tgagaaaca cgtcactgt aacctacta tcgatgttt tttacgttt tttgtaaaa attattgtt gcttactct cgtaggctt agttcaacc tattgatgt ttgctgttt ctagtgctc gggcactca gaactcgat tctgatggt cacaatttt tctttgtga cgaactata ttttgaata cgcgaccaa gagaacgaa cctgaagat aatactcta agagcagaa acttatgtt acaacttta cgtgataaa gatagttag ggttatagt aatatattg taagctttt attattttt tcaatgtta ccactacac aatcgtgtg gaaccacta atatatgcg atataccta gtcaaggca ctgtacata cttaaaaaa atattttat ttacacaaa aagaattct atattttaa tagttatgt aataaatta cctgtcggg agttacctg caactattt aatttttat attcgcttt atttgtgta tttttatat gaaactaaa gaatagtcg agagctttc cccttgtaa atgttgacc aaaattgta gaattgatt cctagtttg agggggttt tactaattt cgcgctagt taccagtcg acttgagaa ttaaatgtg ccatttcat atctataat ttcatatca acaactgcc ttggatcgt aacggatgg actgaaaag agagttacc tgaaggaat ggtcgatat ttgggtgtt acctgcacc tttattctt taaaaaact tacaccgtc cgcttataa tattatagt cttaaagaa aatatacaa gagctttag ttgatgtcc ttttcgcac taacggctt cgttcaagg aatttaaga aaataattt attgtaaat aagatgcta atatagaga tataattgc tattttttt agcatacgg ttcgggcaa acgggaacc ttgcaatat tcgtcatct ctagaattt tgtacctgc tttatttct atctgtttt gtatatttt ggagaaaat atgtgtact taatcgacg atcgctatg gattatttt ccaaaaaga actttttta ccatgttat aggcattaa tactttttt ttgataggt taaccacct atcgtactt aaagcacaa cttgttaac tacatttta taaatttat tattgacat gaaacttgc cattaaatg ccagttttt acttgctgt attgactag attagattt tttaagttt taaattatg ttagatata gttaccaac aaatcctat attctacag ctctgtaga ttatgtgta atcgtatgt gttagtttt tacctgatt ctatcgaaa agtaatgat atacttaag tgaacaatt actacagca attatttcg cagatttag atatatcat tataatgta tttaaccat ttacagttt acttattta tcatatcat tgcctttta cctttttct ttttgttag gtatatgaa atatattgt aacaatttt atttgattt aataaaagt tatgaagta ac
