Status
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Gene information
General information
TC031157
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013145,TC013146
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13230082..13256368
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031157
    iBeetle screen iB_05390

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031157 RA
      MRSGPPTLR QHFRLQQHF NAPQQTHTD SNNNSPAYN 
      FHLQNKCTV LVQGEVSSA PLVAASAQP SAPTSPQSS 
      MLLLQTQTP FGSSSFADL LSAPFSDDP SSVLPPEDL 
      DPFPDVALQ SQPASPAPL PSFQETYSV RYNAPFKMD 
      EECFNVSYH HPPHHVSAP HHYDYQQLQ YTASSPYYP 
      PPQTCSPGF DTLISQETY SLPAYQTAT SELHVSTAL 
      SPRQRRASL PLQRSESTS SSESPKLRM PGPSASSSA 
      ASSPGGIAE PPSKGPTPP SPSQLCAVC GDTAACQHY 
      GVRTCEGCK GFFKRTVQK GSKYVCLAD KACPVDKRR 
      RNRCQFCRF QKCLAVGMV KEVVRTDSL KGRRGRLPS 
      KPKSPQESP PSPPVSLIT ALVRAHVDT TPDLANLDY 
      SQYLEPSPI EPVISEAEK IQQFYTLLT TSVDVIKNF 
      ADKIPGFQE LCPEDRELL FQSASLELF VLRLAYRTH 
      PNDTKLTFC NGVVLDKQQ CQRSFGDWL HAIMEFCSS 
      LMAMEIDIS AFACLCALT LITERHGLK EPHRVEQLQ 
      MKIISSLRD HVTYNAEAQ RKAHYFSRL LGKLPELRS 
      LSVQGLQRI FYLKLEDLV PAPPLIEKM FVASLPF
    CDS sequence
    • >TC031157 RA
      atgcgctca ggtcctccg actctacgg cagcacttc 
      aggcttcag caacacttt aacgcgccg caacagacg 
      cacactgac tccaacaac aactcgccc gcatataac 
      ttccatctt cagaacaag tgtacagtg ttagtacag 
      ggcgaagtc tcaagtgcc cccctggtg gcggcgagc 
      gcgcagcct tccgcccca actagtccc cagtcaagt 
      atgctcctg ctccagaca cagaccccg tttggctcg 
      agcagtttc gcggatctg ctgagtgcg cccttttcg 
      gacgacccg agcagcgtc ttgccccct gaagacctc 
      gaccccttc ccggacgtc gccctacag tcgcagccg 
      gcctctccg gcgccgctt cccagcttc caggagacc 
      tactcagtc cgttacaac gctccgttc aaaatggac 
      gaagaatgc ttcaacgtc tcctaccac cacccccca 
      caccacgtc tccgcgccg caccattac gactaccaa 
      caacttcaa tacaccgct tccagccct tactacccg 
      cccccgcag acctgcagt cccggcttc gacaccctc 
      atctcccaa gaaacctac tccctccca gcctaccaa 
      accgccacg tcggaactc cacgtcagc accgcttta 
      agcccccgc caacgtcgc gcctctttg cccctccaa 
      cgctccgaa agcaccagc agcagcgag agccccaaa 
      ctccggatg cccggaccc tccgccagc tcctcggcg 
      gcgagttcc cccggaggc atcgccgag ccgccctcc 
      aaaggcccc acacccccg tccccatcc caactctgc 
      gccgtctgc ggcgacacc gccgcctgc cagcactac 
      ggcgtccgc acctgcgag ggctgcaag ggcttcttc 
      aaacgcacc gtccagaaa ggctccaag tacgtgtgt 
      ctcgccgac aaggcctgc cctgtggac aagaggcgg 
      cggaaccgg tgccagttc tgccgcttc cagaagtgt 
      ctcgccgtg gggatggtc aaggaggtc gtccggacg 
      gactccctg aagggcagg agaggcaga ctgccgtcg 
      aaacccaag tctccgcag gaatcacca ccgagtcct 
      cccgtctca ctcatcaca gctttagtt cgcgcacat 
      gtcgacacc acccctgat ctcgctaat ttagactac 
      tcgcaatac ctagagccc agtccgatc gagccggtc 
      atctccgaa gccgagaag atccagcag ttctacacc 
      ctgctgacc acctcggtg gacgtgatc aagaacttc 
      gccgacaag atccccggc ttccaggag ctgtgtccc 
      gaagaccgg gagctcctg ttccagtcc gcctctctg 
      gagctgttc gtcctccgg ctggcttac cgcacgcac 
      ccgaacgac accaaactg acgttctgc aacggcgtc 
      gtgctggac aagcagcag tgtcaaagg tccttcggt 
      gattggttg catgccatc atggagttc tgcagcagc 
      ttgatggcc atggagatt gatatttcg gcgttcgcc 
      tgtctgtgt gcactcacc ctcatcacc gagcggcac 
      ggtctgaag gagccccac cgtgtggag cagctccaa 
      atgaagata atctcctcg ctgagggat catgtgacg 
      tacaacgcg gaggcgcag aggaaggcg cactacttt 
      agccggcta ttgggcaaa ctgccggag ctgaggtcc 
      ttgagcgtg caaggactg cagaggatc ttttacctg 
      aagctggaa gacttggtc ccggcccct cccctcatc 
      gagaagatg ttcgtagct agcctacct ttctaa
    mRNA sequence
    • >TC031157 RA
      gtgcgtatc tcgcaagaa gttggaccg caagcagca 
      aagtgcaag aagtgcatc gcttcgcgg actgaaata 
      gtattggtt tcagcgcga cgcggtttc agtgagctt 
      tgtggtgga gtgatcttg ccgttatta tgcttgttt 
      tatgaagat actgttcgc agtggattc gcctgaaac 
      gaaggaagg cgcgcgctc ttctaccaa ttcaccgga 
      gtcctcgct ccgggagtc gccaggtga tttgttcga 
      tgttggtag ttgatgctc acttttgtc cgcctgctg 
      tgttttttc gttgtttat gcatgtgga ctagagact 
      agtttagag gattattgc gatgcaacc gttacgaga 
      tgcgctcag gtcctccga ctctacggc agcacttca 
      ggcttcagc aacacttta acgcgccgc aacagacgc 
      acactgact ccaacaaca actcgcccg catataact 
      tccatcttc agaacaagt gtacagtgt tagtacagg 
      gcgaagtct caagtgccc ccctggtgg cggcgagcg 
      cgcagcctt ccgccccaa ctagtcccc agtcaagta 
      tgctcctgc tccagacac agaccccgt ttggctcga 
      gcagtttcg cggatctgc tgagtgcgc ccttttcgg 
      acgacccga gcagcgtct tgccccctg aagacctcg 
      accccttcc cggacgtcg ccctacagt cgcagccgg 
      cctctccgg cgccgcttc ccagcttcc aggagacct 
      actcagtcc gttacaacg ctccgttca aaatggacg 
      aagaatgct tcaacgtct cctaccacc accccccac 
      accacgtct ccgcgccgc accattacg actaccaac 
      aacttcaat acaccgctt ccagccctt actacccgc 
      ccccgcaga cctgcagtc ccggcttcg acaccctca 
      tctcccaag aaacctact ccctcccag cctaccaaa 
      ccgccacgt cggaactcc acgtcagca ccgctttaa 
      gcccccgcc aacgtcgcg cctctttgc ccctccaac 
      gctccgaaa gcaccagca gcagcgaga gccccaaac 
      tccggatgc ccggaccct ccgccagct cctcggcgg 
      cgagttccc ccggaggca tcgccgagc cgccctcca 
      aaggcccca cacccccgt ccccatccc aactctgcg 
      ccgtctgcg gcgacaccg ccgcctgcc agcactacg 
      gcgtccgca cctgcgagg gctgcaagg gcttcttca 
      aacgcaccg tccagaaag gctccaagt acgtgtgtc 
      tcgccgaca aggcctgcc ctgtggaca agaggcggc 
      ggaaccggt gccagttct gccgcttcc agaagtgtc 
      tcgccgtgg ggatggtca aggaggtcg tccggacgg 
      actccctga agggcagga gaggcagac tgccgtcga 
      aacccaagt ctccgcagg aatcaccac cgagtcctc 
      ccgtctcac tcatcacag ctttagttc gcgcacatg 
      tcgacacca cccctgatc tcgctaatt tagactact 
      cgcaatacc tagagccca gtccgatcg agccggtca 
      tctccgaag ccgagaaga tccagcagt tctacaccc 
      tgctgacca cctcggtgg acgtgatca agaacttcg 
      ccgacaaga tccccggct tccaggagc tgtgtcccg 
      aagaccggg agctcctgt tccagtccg cctctctgg 
      agctgttcg tcctccggc tggcttacc gcacgcacc 
      cgaacgaca ccaaactga cgttctgca acggcgtcg 
      tgctggaca agcagcagt gtcaaaggt ccttcggtg 
      attggttgc atgccatca tggagttct gcagcagct 
      tgatggcca tggagattg atatttcgg cgttcgcct 
      gtctgtgtg cactcaccc tcatcaccg agcggcacg 
      gtctgaagg agccccacc gtgtggagc agctccaaa 
      tgaagataa tctcctcgc tgagggatc atgtgacgt 
      acaacgcgg aggcgcaga ggaaggcgc actacttta 
      gccggctat tgggcaaac tgccggagc tgaggtcct 
      tgagcgtgc aaggactgc agaggatct tttacctga 
      agctggaag acttggtcc cggcccctc ccctcatcg 
      agaagatgt tcgtagcta gcctacctt tctaaattg 
      ttagttgat atttttcga aacgtgata aactcttct 
      atgtgattt gttaaatag gtcgtacgt attagtctt 
      tcccgaggg cgagggaac tgcccgaaa atccggtca 
      cggcctaaa aaagacgct tttttggga cgatgatgg 
      tggggctgg tggactttc gggcagttt cgccagatt 
      tcgaattat gttgagaac ttttggtgt tgcagttct 
      tgtgcacga tagacacct tttccactt tgagaaaca 
      cgtcactgt aacctacta tcgatgttt tttacgttt 
      tttgtaaaa attattgtt gcttactct cgtaggctt 
      agttcaacc tattgatgt ttgctgttt ctagtgctc 
      gggcactca gaactcgat tctgatggt cacaatttt 
      tctttgtga cgaactata ttttgaata cgcgaccaa 
      gagaacgaa cctgaagat aatactcta agagcagaa 
      acttatgtt acaacttta cgtgataaa gatagttag 
      ggttatagt aatatattg taagctttt attattttt 
      tcaatgtta ccactacac aatcgtgtg gaaccacta 
      atatatgcg atataccta gtcaaggca ctgtacata 
      cttaaaaaa atattttat ttacacaaa aagaattct 
      atattttaa tagttatgt aataaatta cctgtcggg 
      agttacctg caactattt aatttttat attcgcttt 
      atttgtgta tttttatat gaaactaaa gaatagtcg 
      agagctttc cccttgtaa atgttgacc aaaattgta 
      gaattgatt cctagtttg agggggttt tactaattt 
      cgcgctagt taccagtcg acttgagaa ttaaatgtg 
      ccatttcat atctataat ttcatatca acaactgcc 
      ttggatcgt aacggatgg actgaaaag agagttacc 
      tgaaggaat ggtcgatat ttgggtgtt acctgcacc 
      tttattctt taaaaaact tacaccgtc cgcttataa 
      tattatagt cttaaagaa aatatacaa gagctttag 
      ttgatgtcc ttttcgcac taacggctt cgttcaagg 
      aatttaaga aaataattt attgtaaat aagatgcta 
      atatagaga tataattgc tattttttt agcatacgg 
      ttcgggcaa acgggaacc ttgcaatat tcgtcatct 
      ctagaattt tgtacctgc tttatttct atctgtttt 
      gtatatttt ggagaaaat atgtgtact taatcgacg 
      atcgctatg gattatttt ccaaaaaga actttttta 
      ccatgttat aggcattaa tactttttt ttgataggt 
      taaccacct atcgtactt aaagcacaa cttgttaac 
      tacatttta taaatttat tattgacat gaaacttgc 
      cattaaatg ccagttttt acttgctgt attgactag 
      attagattt tttaagttt taaattatg ttagatata 
      gttaccaac aaatcctat attctacag ctctgtaga 
      ttatgtgta atcgtatgt gttagtttt tacctgatt 
      ctatcgaaa agtaatgat atacttaag tgaacaatt 
      actacagca attatttcg cagatttag atatatcat 
      tataatgta tttaaccat ttacagttt acttattta 
      tcatatcat tgcctttta cctttttct ttttgttag 
      gtatatgaa atatattgt aacaatttt atttgattt 
      aataaaagt tatgaagta ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY