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Gene information
General information
TC013124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13486292..13487851
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013124
    iBeetle screen iB_05388

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: The yolk in some of the eggs shows clearly more fluorescence than normal.
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013124 RA
      MTRTKLFIL FGILNAVNI ISAGRTPLK VKWVELTAI 
      EETKKPETD SKPYPAAVA TLEVLKPYP PAPVSVAPI 
      LNIANSFPY PPATIVQPQ IVEEIKTPV VTEIHYNAP 
      AVDIVKPYP PAAPVEVVQ PVVHEQVAQ FPYPAAVVE 
      EIQPIVTEY NYNFPPVAP VEIVQPVVS THPYPPAVA 
      DEIKTPIVT DYHYNLPAV EIVKPYPPA APVEVVQPV 
      VPEPYVPAI EEIKTPIVT EYHYNLPAA ETLKPYPPA 
      APVEVVQPV VSTHPYPPA VVEETKTPI VTEYHYDLP 
      TVEIVKPYP AAPVEVVQP VVHEQVATF PYPAAVVEE 
      NKTPIVTEY HYNLPAGEI LKPYPPAAV EVVPEPVAT 
      FPYAPAVNK IQPVVTEYQ IPAVEIVKP APQVVATFP 
      YPAAVVEEI KTPILPPPA AETLYPYPP AVIQTAHPV 
      VNELLATYP YPAAVPAEA VHIREPRKI TENSNQSQM
    CDS sequence
    • >TC013124 RA
      atgacgcgc actaaatta tttatacta ttcgggata 
      ttaaatgcc gttaacata atttctgcg ggccgaact 
      cctttgaaa gtaaaatgg gttgaatta actgctatc 
      gaagaaaca aaaaaaccg gaaaccgat tcaaaacca 
      tatcctgcg gctgtcgca acactggaa gttttaaaa 
      ccatatcca ccagctccc gtgagtgtt gctccaatt 
      ctaaatatc gctaattca ttcccttat cctccggct 
      accattgta caaccccaa attgtcgaa gaaattaaa 
      actccggtt gtaacagaa atccattac aacgcacct 
      gctgtagac atagtaaag ccatatcct cctgcagcc 
      cctgtagaa gttgtacaa cctgttgtt cacgaacaa 
      gttgcacaa ttcccctac cctgctgct gttgttgaa 
      gaaattcaa ccgattgta accgaatac aactacaat 
      tttcctcct gtggctcct gtagaaatt gtacaacct 
      gttgtttct acacatccc tatccacct gctgttgcc 
      gatgagata aaaacacct attgtaacc gattatcac 
      tacaacctt cctgctgtg gaaatagtg aaaccctac 
      ccccctgca gctcctgtc gaagttgta caacctgtt 
      gtaccagaa ccgtacgtt cccgctatt gaagaaatt 
      aaaactccg attgtaact gaataccac tacaatctt 
      cctgctgcc gaaacatta aaaccgtat cctcctgca 
      gcacctgta gaagttgta caacctgtt gtttctaca 
      cacccctat ccacctgct gttgtcgag gagacaaaa 
      accccaatt gtaaccgaa tatcactac gaccttcct 
      actgtagaa atagtgaaa ccctatcct gcagctcct 
      gtagaagtt gtacaacct gttgttcac gaacaagtc 
      gcaacattc ccctaccct gctgctgtt gtggaagaa 
      aataaaacc ccgattgta accgaatat cactacaac 
      cttcctgct ggagaaata ttaaaaccg tatcctcca 
      gcagctgta gaagttgta cctgaacca gtcgctaca 
      tttccctat gctcctgct gttaacaaa attcaaccg 
      gttgtgacc gaatatcaa attcctgca gtagaaata 
      gtaaaacct gctccccaa gttgtagca acattccct 
      tatcctgca gcagttgta gaagaaata aaaactcca 
      attcttcca cctcctgcc gcagaaacc ctatatcca 
      tacccacca gcagttata caaacagcc caccctgtg 
      gttaatgaa ctgttagct acgtatcca tacccagct 
      gctgttcct gctgaagct gtgcacata agagaaccg 
      cgaaaaata acagaaaat tcaaaccaa tcgcaaatg 
      taa
    mRNA sequence
    • >TC013124 RA
      ataattgtt tttttggtg ataacacaa cgatgacgc 
      gcactaaat tatttatac tattcggga tattaaatg 
      ccgttaaca taatttctg cgggccgaa ctcctttga 
      aagtaaaat gggttgaat taactgcta tcgaagaaa 
      caaaaaaac cggaaaccg attcaaaac catatcctg 
      cggctgtcg caacactgg aagttttaa aaccatatc 
      caccagctc ccgtgagtg ttgctccaa ttctaaata 
      tcgctaatt cattccctt atcctccgg ctaccattg 
      tacaacccc aaattgtcg aagaaatta aaactccgg 
      ttgtaacag aaatccatt acaacgcac ctgctgtag 
      acatagtaa agccatatc ctcctgcag cccctgtag 
      aagttgtac aacctgttg ttcacgaac aagttgcac 
      aattcccct accctgctg ctgttgttg aagaaattc 
      aaccgattg taaccgaat acaactaca attttcctc 
      ctgtggctc ctgtagaaa ttgtacaac ctgttgttt 
      ctacacatc cctatccac ctgctgttg ccgatgaga 
      taaaaacac ctattgtaa ccgattatc actacaacc 
      ttcctgctg tggaaatag tgaaaccct acccccctg 
      cagctcctg tcgaagttg tacaacctg ttgtaccag 
      aaccgtacg ttcccgcta ttgaagaaa ttaaaactc 
      cgattgtaa ctgaatacc actacaatc ttcctgctg 
      ccgaaacat taaaaccgt atcctcctg cagcacctg 
      tagaagttg tacaacctg ttgtttcta cacacccct 
      atccacctg ctgttgtcg aggagacaa aaaccccaa 
      ttgtaaccg aatatcact acgaccttc ctactgtag 
      aaatagtga aaccctatc ctgcagctc ctgtagaag 
      ttgtacaac ctgttgttc acgaacaag tcgcaacat 
      tcccctacc ctgctgctg ttgtggaag aaaataaaa 
      ccccgattg taaccgaat atcactaca accttcctg 
      ctggagaaa tattaaaac cgtatcctc cagcagctg 
      tagaagttg tacctgaac cagtcgcta catttccct 
      atgctcctg ctgttaaca aaattcaac cggttgtga 
      ccgaatatc aaattcctg cagtagaaa tagtaaaac 
      ctgctcccc aagttgtag caacattcc cttatcctg 
      cagcagttg tagaagaaa taaaaactc caattcttc 
      cacctcctg ccgcagaaa ccctatatc catacccac 
      cagcagtta tacaaacag cccaccctg tggttaatg 
      aactgttag ctacgtatc catacccag ctgctgttc 
      ctgctgaag ctgtgcaca taagagaac cgcgaaaaa 
      taacagaaa attcaaacc aatcgcaaa tgtaacgct 
      tgtgtctgc tgtttattt attgtataa ttgattttt 
      taataaatt gatttgtaa aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY