Status
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Gene information
General information
TC013108 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13650980..13657209
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013108
    iBeetle screen iB_05385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013108 RA
      MQIIELQQS DYDEAYTNG VSVSNVDVA YRKITKTST 
      AFLIWRVEN MSIVAVPRD QYGIFYDTD CYVIFASSP 
      YGQPVGVDS VSREVKGTP LEYHIHFWL GCSTTPDKS 
      GVVAYKTVE LDNFLNGTA IQHRETQGN ESPRFKSYF 
      KSGFRILTS EFSLLTLPK LYKVKGKCT PVLIQMDNI 
      TWEKFNSSD IFVLHTPNV LFVWVGRAS DAAEKLNAA 
      KLATEMKEQ YNIANIVFV DDGYEKTLQ DDEKKEWNK 
      CLPLEKRHV LPENESETL NFFQRSNNI RLYKCSENN 
      GKYRVAEIK SGPLYQCDL DADEVFIID QEIHGIWIW 
      VGKRASDKE RGEALRNAR GFVKKKKYP NNTNVTRVV 
      DGFESSEFK MLFSFWKDE TNKANGRGG KPTVLVSKF 
      DAVTMEERP SLAAETQLI DDGSGSVTL WRIKQHNLV 
      EIPKERHGF FFNGDCYIV LYSYQTSAE ERHLLYYWL 
      GSHATQEEI TYTNAKVLE IDEELGGLG FQARVIQGR 
      EPAHFLQLF KGKLIVFKG KGTDFDESG RNLKHPMQY 
      FLQVFGSTL AGSKAVQIQ PRASHLNSN YCYVFKRGK 
      HAFIWCGHY STGDQREMA KLFAGKDFE LVLEGKEKP 
      EFFDLLGGK AVYATQLVR DDGDVRPPR LFHCAKING 
      VLRAEEIFF FNQNDLLPE NVMLVDFFT VLYLWIGNL 
      SSKEDQRQS LLVALEYLQ TDPCGRDMN IPIIQISQG 
      YEPPTFTGF FPSWDNNLW KSYKSFGKR RQDIEMKNA 
      KMNGKEING YQKTSGHSD FDQYDKYPL NILKEPNDK 
      LPARVDPLN KELHLTHDD FVSVLKMTY LEFEKLPRW 
      KQQELKKRA GLF
    CDS sequence
    • >TC013108 RA
      atgcaaatc attgaactg caacagagt gattatgac 
      gaagcttac acgaatggg gtaagtgtg agcaatgtg 
      gacgtcgcc tatcgcaaa attaccaaa acctcgaca 
      gctttcctg atatggcgc gtcgaaaac atgagtatt 
      gttgcggtc ccccgggac caatacggg attttttac 
      gacacggat tgctacgtc atttttgcg tcttccccc 
      tacggacaa ccagtcggg gttgattcg gtgtctcgc 
      gaggttaaa ggaactccc ctggagtac cacatccac 
      ttctggctg ggttgttcc acaactccg gacaagtcc 
      ggagtcgtc gcttacaaa accgtggaa ctggataat 
      ttcctcaac ggaacggca atccagcat cgagaaacg 
      caaggaaat gaaagtcca aggtttaag agctacttc 
      aagagcgga tttaggatt ttaacatcg gaattttcc 
      ttattaacg ctccccaag ctttacaaa gtcaagggg 
      aagtgcacc cccgtcctg atccaaatg gacaacatc 
      acctgggag aagttcaac agcagcgat atcttcgtc 
      ctgcacacg cccaacgtt ttgttcgtt tgggtcggg 
      agggcttca gacgccgcg gaaaaatta aacgcagcc 
      aaactggca acggaaatg aaagaacag tataacatt 
      gcaaatatt gtttttgtg gacgacggt tatgaaaaa 
      accctgcaa gacgatgag aaaaaagag tggaacaaa 
      tgtttgcct ttggagaaa cggcacgtc ctacctgaa 
      aatgaatcc gaaacgctg aattttttc caaagatcg 
      aacaacatc cgactttac aagtgttca gagaacaac 
      ggcaagtac agggtggct gaaattaag agcgggcct 
      ttgtatcaa tgcgacttg gacgctgat gaggttttt 
      ataattgat caggagata cacgggatt tggatttgg 
      gtcgggaag agggccagt gataaggaa cgaggggaa 
      gccctcagg aacgcccgc ggttttgtt aagaaaaag 
      aaatatccc aacaacact aacgtgact cgagttgtt 
      gacggtttc gagtctagc gaattcaaa atgctcttt 
      tccttctgg aaagacgaa actaacaaa gccaatggt 
      cgaggcgga aaaccgacc gtcttggtg tcaaaattc 
      gacgccgtg acgatggag gagcgccca agtctcgct 
      gcagaaacg cagcttatt gacgacggt tcaggaagt 
      gtgacctta tggcgaata aaacagcac aacctggtc 
      gaaataccg aaagaacgt cacggtttc tttttcaat 
      ggcgattgt tacattgtc ttgtacagc tatcaaaca 
      tcagcggaa gaaagacat ttactttat tactggttg 
      ggatcccac gccacgcaa gaagaaatc acttacaca 
      aacgccaaa gtgctggag attgacgaa gagttggga 
      gggctgggg ttccaagcc cgtgtgatc caagggcgg 
      gaacctgcc cactttttg caactgttt aagggaaaa 
      ctgatcgtg ttcaaaggg aaaggcacc gattttgat 
      gaatccggg cgtaatttg aagcacccg atgcagtat 
      ttcctccaa gtttttggc tccacttta gccgggagt 
      aaagccgtg caaattcag ccgagagcc tcgcacttg 
      aactcgaac tattgctac gtgtttaaa agaggcaaa 
      cacgcgttt atttggtgc ggacattat tctactgga 
      gaccagagg gaaatggcg aaattgttc gccggcaaa 
      gatttcgag cttgtgtta gaagggaag gaaaagccg 
      gaatttttc gacctgttg ggtgggaaa gcggtttac 
      gcgacacag ctagtgagg gatgatggg gacgtgcga 
      ccaccgaga cttttccat tgtgcgaaa attaacggt 
      gtcttgaga gcggaagaa attttcttt ttcaaccag 
      aacgatctt ctacccgag aacgtcatg ctggtggac 
      tttttcacc gtgctttat ttgtggatt gggaatttg 
      agttcaaag gaagaccaa agacagagt ttgcttgtg 
      gctcttgaa tatttgcag actgatcct tgtggacga 
      gacatgaat ataccgata attcaaata agtcaagga 
      tatgagccc ccaacgttt acaggattt ttcccatcg 
      tgggacaat aatttatgg aagtcgtat aaatcattc 
      ggtaagcgg agacaagac atagaaatg aaaaatgcg 
      aaaatgaac gggaaagaa ataaacggt tatcagaag 
      acatcaggg catagtgat tttgatcag tacgataag 
      tatccgtta aatatcctt aaggaaccg aatgataag 
      cttcctgcg agggtagat cctttaaac aaagagtta 
      cacctaacc catgacgac ttcgtctcg gttttgaag 
      atgacttat ctcgagttt gagaagttg ccgcgttgg 
      aaacagcaa gagttgaaa aagcgtgca ggacttttc 
      tga
    mRNA sequence
    • >TC013108 RA
      tcacttacc ttacagtgg catttccaa aactcataa 
      tttgatcaa gcggcaaaa ttcttcgac aaacactaa 
      actggaaaa actgatttg atttgccgc acatttcgg 
      cagcaatgc gtatttccg ttcttcgct tcggaagtt 
      aactttggt catttccca aaacaatta ccattcaaa 
      ccatcgaca gcgcaattc caaaactca cttctgtac 
      ctatttgca agggcactt tgattcagg tagtaccat 
      cagtcagtg ttaattagt gaattgctt ggtgtgtca 
      caggcaatt atgaaggag tctcggaag aagacttcc 
      gtaagttat taaaagctt aaccaacct taaaaacct 
      ggctgcaat taaacataa taacgccat tttgcatgt 
      caaggcgta attagtgcg ggcaaaagg tgacaaaag 
      ggtcgcatt ccgaaaatc ttgctcctg gagtcacgg 
      agcgtgaag agatcattt ttctattga attatattt 
      aattagttt ttaatgtac cgctacgtc tcgagaggc 
      tcaaaaatt ggaaaaagt tgattacgg gtactgcta 
      ggaacgaaa cagtttaat tacaccaaa tatttatac 
      gacattaca gtaaataac aaagcggcc attaatcaa 
      tccaccgtt taaaaacgg ccaattaat tctatgaac 
      taatttact tatcgttac acaaaaaat tccatattt 
      cattgtcag gcgactatc taggtggag cattttcct 
      aattggtgc aactattga aaaatcagc cgcactttc 
      agttttctg gacgaagcg ttgcgtaac taattctcg 
      aaatggcaa acgattcga aggggctct aatcaaatt 
      tgttgcagc aattatttg attaatggt tctagaacg 
      aaatttatt tttttaaat tggaccgtt ttgtaaaag 
      tctcggcta cggaagaat aggaggggg tcactcact 
      tcaaagatc caaagaaac ttgcgattt gccggttgt 
      tgtccgcat tctatgtca agacgtgaa gttaaatta 
      taccgcggc tcgcattat cttgtaaat ttcgttaca 
      aagtcgcta gtttagtga tgcagtgcc agtgaacaa 
      ccaaaatgc aaatcattg aactgcaac agagtgatt 
      atgacgaag cttacacga atggggtaa gtgtgagca 
      atgtggacg tcgcctatc gcaaaatta ccaaaacct 
      cgacagctt tcctgatat ggcgcgtcg aaaacatga 
      gtattgttg cggtccccc gggaccaat acgggattt 
      tttacgaca cggattgct acgtcattt ttgcgtctt 
      ccccctacg gacaaccag tcggggttg attcggtgt 
      ctcgcgagg ttaaaggaa ctcccctgg agtaccaca 
      tccacttct ggctgggtt gttccacaa ctccggaca 
      agtccggag tcgtcgctt acaaaaccg tggaactgg 
      ataatttcc tcaacggaa cggcaatcc agcatcgag 
      aaacgcaag gaaatgaaa gtccaaggt ttaagagct 
      acttcaaga gcggattta ggattttaa catcggaat 
      tttccttat taacgctcc ccaagcttt acaaagtca 
      aggggaagt gcacccccg tcctgatcc aaatggaca 
      acatcacct gggagaagt tcaacagca gcgatatct 
      tcgtcctgc acacgccca acgttttgt tcgtttggg 
      tcgggaggg cttcagacg ccgcggaaa aattaaacg 
      cagccaaac tggcaacgg aaatgaaag aacagtata 
      acattgcaa atattgttt ttgtggacg acggttatg 
      aaaaaaccc tgcaagacg atgagaaaa aagagtgga 
      acaaatgtt tgcctttgg agaaacggc acgtcctac 
      ctgaaaatg aatccgaaa cgctgaatt ttttccaaa 
      gatcgaaca acatccgac tttacaagt gttcagaga 
      acaacggca agtacaggg tggctgaaa ttaagagcg 
      ggcctttgt atcaatgcg acttggacg ctgatgagg 
      tttttataa ttgatcagg agatacacg ggatttgga 
      tttgggtcg ggaagaggg ccagtgata aggaacgag 
      gggaagccc tcaggaacg cccgcggtt ttgttaaga 
      aaaagaaat atcccaaca acactaacg tgactcgag 
      ttgttgacg gtttcgagt ctagcgaat tcaaaatgc 
      tcttttcct tctggaaag acgaaacta acaaagcca 
      atggtcgag gcggaaaac cgaccgtct tggtgtcaa 
      aattcgacg ccgtgacga tggaggagc gcccaagtc 
      tcgctgcag aaacgcagc ttattgacg acggttcag 
      gaagtgtga ccttatggc gaataaaac agcacaacc 
      tggtcgaaa taccgaaag aacgtcacg gtttctttt 
      tcaatggcg attgttaca ttgtcttgt acagctatc 
      aaacatcag cggaagaaa gacatttac tttattact 
      ggttgggat cccacgcca cgcaagaag aaatcactt 
      acacaaacg ccaaagtgc tggagattg acgaagagt 
      tgggagggc tggggttcc aagcccgtg tgatccaag 
      ggcgggaac ctgcccact ttttgcaac tgtttaagg 
      gaaaactga tcgtgttca aagggaaag gcaccgatt 
      ttgatgaat ccgggcgta atttgaagc acccgatgc 
      agtatttcc tccaagttt ttggctcca ctttagccg 
      ggagtaaag ccgtgcaaa ttcagccga gagcctcgc 
      acttgaact cgaactatt gctacgtgt ttaaaagag 
      gcaaacacg cgtttattt ggtgcggac attattcta 
      ctggagacc agagggaaa tggcgaaat tgttcgccg 
      gcaaagatt tcgagcttg tgttagaag ggaaggaaa 
      agccggaat ttttcgacc tgttgggtg ggaaagcgg 
      tttacgcga cacagctag tgagggatg atggggacg 
      tgcgaccac cgagacttt tccattgtg cgaaaatta 
      acggtgtct tgagagcgg aagaaattt tctttttca 
      accagaacg atcttctac ccgagaacg tcatgctgg 
      tggactttt tcaccgtgc tttatttgt ggattggga 
      atttgagtt caaaggaag accaaagac agagtttgc 
      ttgtggctc ttgaatatt tgcagactg atccttgtg 
      gacgagaca tgaatatac cgataattc aaataagtc 
      aaggatatg agcccccaa cgtttacag gatttttcc 
      catcgtggg acaataatt tatggaagt cgtataaat 
      cattcggta agcggagac aagacatag aaatgaaaa 
      atgcgaaaa tgaacggga aagaaataa acggttatc 
      agaagacat cagggcata gtgattttg atcagtacg 
      ataagtatc cgttaaata tccttaagg aaccgaatg 
      ataagcttc ctgcgaggg tagatcctt taaacaaag 
      agttacacc taacccatg acgacttcg tctcggttt 
      tgaagatga cttatctcg agtttgaga agttgccgc 
      gttggaaac agcaagagt tgaaaaagc gtgcaggac 
      ttttctgaa taaggtttc atctaatga tggcggcca 
      gcgagtaga ataagacgc attgtttat ttgtaaact 
      catttattg taacatcgt ttaataatt ataataaat 
      aaacaaata tatgacaat tataacaag aaacaagaa 
      taaatagtt ataatataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY