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Gene information
General information
TC013080 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14259841..14263417
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013080
    iBeetle screen iB_05380

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: about 50%
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013080 RA
      MSLGRGIFS VDAASTGNS SALPPHSIL ATARATPPS 
      LPPPPGSSH SPTRRLVLQ QFASSSRRH RRRCASSMT 
      AKSKRASEP RRANKPLME KRRRARINQ SLAALKTLI 
      LDSAKADNT KHSKLEKAD ILELTVRHF QRHRSLDIK 
      GVHQYKAGY ADCVREVQR YLDTPDAQT MTVVDAGVR 
      QRLLRHLDN CVAEVDVDV RNAGLPPPE ERLQPPDST 
      LEEVNNNQN RSEEPKEDT TAKNFVLLL PEHYLQLAN 
      ALGVNLRHS EGGADSGAS SGESSDSSK QSSGEKPLD 
      FSKSNDNCD MWRPW
    CDS sequence
    • >TC013080 RA
      atgagtctg ggccgaggc atatttagc gtcgatgcc 
      gccagcaca gggaacagc agcgccctt cccccgcac 
      tcgatcctc gctaccgca cgcgccacc cccccgtca 
      ctgccaccg ccgcctggc agctcgcac tcgcccact 
      cgtcggctc gtgctgcag cagttcgcc tcgtcctct 
      cgccggcat cgccgccga tgcgcctct agtatgacc 
      gccaagagc aagcgagcc tccgagcca cgccgcgcc 
      aacaagccc ctcatggag aagcgccgt cgggctcgc 
      atcaaccag agcctggcc gccctcaag accctcatc 
      ctcgactcg gccaaggcc gacaacacc aagcactcc 
      aagctggag aaggccgac atcctggag ctcaccgtg 
      cgccacttc cagcggcac cgcagtctg gacatcaag 
      ggagtgcac cagtacaag gccggctac gcggactgc 
      gtgcgcgag gtgcagcgc tacctggac acgccggac 
      gcccagacg atgaccgtc gtcgacgct ggcgtgcgc 
      cagcggctg ctccggcac ctggacaac tgcgtcgcc 
      gaggtggac gtggacgtg cgaaacgcg ggcttgccg 
      ccccccgag gagcggctc cagcccccg gactcgacg 
      ctcgaagaa gtcaacaat aaccagaac cggagcgag 
      gagccgaag gaggatacg acagcgaag aattttgtt 
      ctgttactt cccgagcat tatctccag ttggccaac 
      gctctgggg gtgaatttg aggcacagt gagggtgga 
      gccgattcc ggggcctcc tcgggggag agtagcgat 
      agttcgaaa cagtcaagt ggggaaaaa ccgctcgat 
      tttagcaaa agtaacgac aattgtgat atgtggagg 
      ccgtggtga
    mRNA sequence
    • >TC013080 RA
      acctacata cgtgcacca ggtgatcgt taaacgaca 
      acgaaacga atttaatta catagatga attaatcgt 
      tttcagtag ttttcttgt ctatgttag aactgcgcg 
      ggtttggct cgctccagg tcgtcctgc atccacatt 
      aaccaagtt tgcgaacgg gctccggcc ctgtccggt 
      cctaaaata ttgaatatt gacatgatg gagtattta 
      tagtgtagg agaacgtgc aaactggcc aaggcatgt 
      cggcagtca aaacaggct ttccacatg gagatcacg 
      tctcgatcc ggcccgaaa aactagcct caagactga 
      ataaatgaa tctcgtgtg catatccga atttaaaat 
      atgagtctg ggccgaggc atatttagc gtcgatgcc 
      gccagcaca gggaacagc agcgccctt cccccgcac 
      tcgatcctc gctaccgca cgcgccacc cccccgtca 
      ctgccaccg ccgcctggc agctcgcac tcgcccact 
      cgtcggctc gtgctgcag cagttcgcc tcgtcctct 
      cgccggcat cgccgccga tgcgcctct agtatgacc 
      gccaagagc aagcgagcc tccgagcca cgccgcgcc 
      aacaagccc ctcatggag aagcgccgt cgggctcgc 
      atcaaccag agcctggcc gccctcaag accctcatc 
      ctcgactcg gccaaggcc gacaacacc aagcactcc 
      aagctggag aaggccgac atcctggag ctcaccgtg 
      cgccacttc cagcggcac cgcagtctg gacatcaag 
      ggagtgcac cagtacaag gccggctac gcggactgc 
      gtgcgcgag gtgcagcgc tacctggac acgccggac 
      gcccagacg atgaccgtc gtcgacgct ggcgtgcgc 
      cagcggctg ctccggcac ctggacaac tgcgtcgcc 
      gaggtggac gtggacgtg cgaaacgcg ggcttgccg 
      ccccccgag gagcggctc cagcccccg gactcgacg 
      ctcgaagaa gtcaacaat aaccagaac cggagcgag 
      gagccgaag gaggatacg acagcgaag aattttgtt 
      ctgttactt cccgagcat tatctccag ttggccaac 
      gctctgggg gtgaatttg aggcacagt gagggtgga 
      gccgattcc ggggcctcc tcgggggag agtagcgat 
      agttcgaaa cagtcaagt ggggaaaaa ccgctcgat 
      tttagcaaa agtaacgac aattgtgat atgtggagg 
      ccgtggtga tttaactgt tgggatttt ttctatcta 
      gtcacactt ttccaagac ttgagctac gctgtattt 
      tattaaacg ctttacaat gttagctaa atgtctgta 
      aataaattg tacaaaacc gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY