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Gene information
General information
TC033254
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013040
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14780671..14788835
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033254
    iBeetle screen iB_05376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033254 RA
      MKFKTFLFV ISFVVPECF SQDSQKCQN QVIDNDKGP 
      NIDWTNHLI KLENESDDR PVNLVFKYT GELSVTFSD 
      EPEFGAKYI DIVAENNQL SFKSNDNYT KIEEDPNIQ 
      SYVIGNTIS FQRKFTLNC DAEQDILNL LINIKDTNN 
      HDPTFAKTS YSYEMPMPL IPNIDLTGF GDDIIVTDY 
      DFTNDNVAF TIDNTAFEV TTKHNNGSN SYTAVVKVK 
      SYTYLSKDT QLTLTATDS GEPPRSSST TIQIKIDES 
      NSLPDIPSV PEASYQFDY AIGDDKKPE LTPLGADII 
      VTASDSSKL TVKLESSDI APDDYFTVE LDKNSQKVT 
      IKPTDKVIE ASPNPVLVL TLVLTLNGK EKDSSKTAI 
      LVNLPTEKI ITIPQFGQA YYEAQYIVN DDKDTVELS 
      TDITVSENA EVTLVDDEK HFSISKVGD KWQVTVLAD 
      LDSSDLKKG DIPLELQAT VVVDKESYV GFATLIVNL 
      PQVDLRFTK PYYTATYTV SEGDALDTL AVDGDIALT 
      SPSGDVTVE LVDNQDYFT LSYIASSGT CTVEVKADL 
      DQPTLHKGG DLIVNFKAT LNGVEELGA LVITLPDVV 
      DAKFVDSNY VANYVITTD GVTVNINGA PISVKPPKP 
      SSQDITVSF KQVNDNFKI VKNVDQYLI SVTTPLNAS 
      DLLTREPKV FTLQTNTVP VATSTLVIE LPDVRPKFT 
      KTSYDATYK VDAAGSATV ELKDTIELI TILTPKLTF 
      TGSFGDHFS FDPDKDPQI SVTTNLDED VVKTNPEIL 
      LTLQATIEG SSETETTII VLKLPNDGT TDTLQFEQP 
      SYVFSYKTG QDKPYIDNG GQSISLVKV HTDAEITIT 
      GTFSENFEL SGTDNQFSL GIKTPLNQT VLDTEPDIL 
      LVLDASLAG LDDGFATIL LHLPEKKSP NGPKFAQAF 
      FTANYELGA DRHDKVTID GKAISLADG TTEANIEPV 
      GTNSEHFKI EKKSDGSGY ELSLVSNLD DSVLIGEAD 
      VVLTLKATL GGDVGEGTL VVKLPKKTA PASFKQPNY 
      VAKYNMDTD PHTIDMDGI ELEGDYLDD TVITLKVDD 
      GLEDYFTIN DKSPYKIAL TKNLDDTVL NSQHEVVVT 
      VVATLDKTS TTTSVIVTI PDLPTPPNF KASVYYGKY 
      DSDKKVVTL DDPIETDAK DSSIVHITA EKPYDTNFA 
      VEYDKTNGF AVKVTQPLD DETVQNNNE IFVVFDATV 
      DRAVAHTSA TLVLKMPTE LNQIVPKFS EAFYNAQLD 
      EKTVTIKSP ITITSDKAG DTQVEVIDD YKEYFGAEF 
      DGTSWQLTV VKELETALT EIVVTLEAS LPGDDIKTT 
      TTVIMALPD HGLTPSPKF TVPLYEAEY VDDEPPSLK 
      ITDPITLQA GFKDATVTV GSNDYQQYF KLAQNKDNA 
      LQWDISLSK PLTDDILKN NAQLVVTLK ADLENSEGP 
      EFATLVIKL PAQSQETVS FGESYYTAD YTVEKDKDK 
      INSSEITVS EGATVTVIK EYTDYFGVS CADKKCVLT 
      VKKNLEDDV LNTKNEIIV VLQATKTDV AEKPTVPVI 
      VSLPLQDSI EAPKFEQIV YTADYDKES STISANSIK 
      FKDTETKGI TVAIDPTSD YKNNFKVTS KTDHWEVEV 
      SQALDVSGK VSISFILQA TKEGVQTMG EAVFVVNLP 
      ANEDTSVVE FSRVTYTGK YNVKDTTDE VVLEQNIQI 
      KGISDTSKA KLIIEGGSN KDNFEIKAS EEQKDQWII 
      GVKTDLPNE ILDTETSIV LTLQATVNE TSSSGEVGV 
      VINLPKAYT PTSFYFTST SYTADYEIK NNSPELKVD 
      SKISIENDP DSTATTVKL SGDFAKYFA ADYSKGIVT 
      ITLSKAIPN EIVEKQSAL PVTIEAAKK DNQVARAVL 
      VVRLPKETS QNDDDDSSD DQTGLIAAV AVLAVLLVA 
      SLVGSAVFY YFKFKTAKN YSNMSDSPR PSERFRKNS 
      AGLDTKHKF EHATRRPTG VIKYPLKDD ESTDCVEEP 
      ISEKRKSVQ FDVIHIDEL EIEPEKTDD EDNENVYEN 
      VDSDSDHRR NSEA
    CDS sequence
    • >TC033254 RA
      atgaagttt aagacattt ttatttgtt atttcgttt 
      gttgtcccg gaatgtttt tcccaagac tctcaaaaa 
      tgccaaaat caggttatc gataacgac aaagggcca 
      aacattgac tggacaaat cacctaata aaattagaa 
      aatgaatct gatgataga cctgtcaat ttagtattt 
      aagtataca ggagaatta agtgtaacg ttttctgat 
      gagcctgaa tttggtgca aaatatata gatatcgtt 
      gcagaaaat aatcaacta agcttcaaa agcaacgat 
      aactataca aagattgag gaagatcca aacatacag 
      tcttacgtt ataggaaac acaataagt tttcaaaga 
      aaattcact ttaaattgt gatgcagag caagatatt 
      ttgaacctt ttaataaac atcaaagat acaaacaac 
      catgatcca acttttgcc aaaaccagt tacagctac 
      gaaatgcct atgccttta atcccaaat attgacttg 
      accggattt ggcgacgat ataattgta acagattac 
      gatttcacc aatgacaat gtagcattt actattgac 
      aatactgct ttcgaagtt acaacaaaa cacaacaat 
      ggttccaac agttacacg gctgttgta aaagttaaa 
      agttataca tatctttca aaggacacg caactgact 
      cttacagca actgattca ggagagccg ccacgatca 
      agctcaacc acaattcaa ataaaaatt gatgaaagc 
      aacagttta cccgacatt ccttcagta ccagaagct 
      tcatatcag tttgactat gctattggt gatgataaa 
      aaaccagaa ctgacacca cttggtgcg gacataata 
      gtaaccgcc agtgattct tccaaactt acagtaaaa 
      ctagaaagt agtgacatt gctcccgat gattatttt 
      accgtcgaa cttgacaaa aattcacaa aaagtaaca 
      ataaagccc actgacaaa gtcatagaa gcttcaccg 
      aaccccgtg ctggtttta accctcgtg ttaactcta 
      aacggaaaa gaaaaagac agcagcaaa accgcgatt 
      ctggtaaac ctcccaact gagaaaatt atcaccata 
      ccacaattc ggtcaagcg tactatgag gcccagtat 
      atagttaac gatgataaa gacaccgtg gagctgtcg 
      acagatatt acagtttcc gaaaacgct gaggtcaca 
      cttgtagat gacgaaaaa cactttagt ataagcaag 
      gttggtgat aagtggcaa gtaactgtg ctggcggat 
      ttggatagt agtgacttg aaaaaaggt gatatcccc 
      ttggaactt caagctaca gtagttgtt gataaagag 
      tcatacgtt ggttttgct actttgatt gttaacctt 
      cctcaagtt gacttgcgg tttactaaa ccttactac 
      acggcaact tacaccgtt agcgagggg gacgccctt 
      gatacgtta gcagtggat ggagacatt gcactgact 
      tcaccaagt ggtgatgtt acagttgaa ttagttgac 
      aatcaggat tatttcaca ttaagttat attgcgagt 
      tctggaaca tgtacggta gaagttaag gctgattta 
      gatcaacct actttgcac aaagggggc gatttgatc 
      gtgaatttc aaagcaaca ctaaacggt gttgaggaa 
      ctaggtgct ttggtcatc acattaccg gatgttgtt 
      gatgcaaaa tttgttgat tccaattat gtggctaat 
      tacgtaatt accaccgat ggagttaca gttaatata 
      aacggggct ccaatctcg gtaaagccc cccaagccc 
      tccagtcaa gacattact gtcagtttc aaacaagtg 
      aatgataat tttaaaatc gtgaaaaac gtggaccaa 
      tatcttatc agtgttaca actccttta aacgcctcg 
      gatttgcta acgcgagaa cctaaagtt ttcactttg 
      caaacaaac accgtaccg gttgctaca agtactttg 
      gttattgaa ttaccagac gttcggccc aaattcacc 
      aaaacttcc tatgacgcc acttataaa gttgatgct 
      gcaggaagt gcaacagtt gaactaaag gacacgatt 
      gaattaatt acaatactg accccgaaa ctcactttt 
      actggctcc tttggagac catttctct tttgatccc 
      gacaaagat ccacaaatt agtgtcaca actaatcta 
      gacgaagac gttgtaaag acaaaccca gaaatttta 
      ctgacacta caagcaact attgagggt tctagcgag 
      actgaaacg acaatcatt gtcctaaaa ttgccaaac 
      gatgggaca acagacacg ttgcaattt gagcaacca 
      tcgtatgtt ttttcgtac aaaactggc caagacaag 
      ccttacatt gacaacggg gggcaaagt ataagtcta 
      gtcaaagta cacactgac gctgaaatt accatcact 
      gggacgttt agtgaaaat ttcgaattg tcaggcacc 
      gataaccaa tttagcctt ggcatcaaa actccctta 
      aaccaaacc gtcctagac accgaacct gacattctt 
      ctcgttctg gacgcaagt ctggccggt ctggacgat 
      ggtttcgcc acgattttg cttcatctc cctgagaaa 
      aaatccccg aatgggcca aaattcgcc caagcgttt 
      tttacagcc aattatgag ttgggagct gaccgacac 
      gataaagtc acaattgac ggaaaggcc ataagtcta 
      gctgatgga acaactgag gcaaacatt gaaccagtc 
      ggaacaaat agcgaacac tttaagatt gagaagaag 
      agtgacggt agtggttat gagctgagt ttggtttcg 
      aatttggat gattcggtt ttgattggt gaagctgat 
      gtggttttg acgttgaaa gcaacgctt ggtggtgac 
      gttggagag ggaactttg gtggtgaaa ctgcccaaa 
      aagacagca ccggcttcg tttaaacaa ccaaactat 
      gtggctaag tacaacatg gacactgat ccacacaca 
      attgacatg gacgggatt gaactcgag ggtgactat 
      ttggacgac actgtgatt actttgaaa gtggatgat 
      ggactcgaa gactatttt acaattaat gacaaaagt 
      ccgtacaaa attgcgtta actaagaat ctggatgat 
      acggtactg aacagtcaa cacgaagtt gtagttact 
      gtagttgca acacttgat aaaacctcg accacaact 
      tccgtgatt gtaacaatc ccagacttg ccaacacca 
      ccaaacttt aaggcgagc gtatactat ggcaaatat 
      gacagtgat aagaaagtt gtaaccctt gatgatcca 
      atcgaaacc gatgccaaa gattcaagt atcgttcac 
      atcactgca gaaaagccc tatgacaca aactttgct 
      gtagagtat gacaaaacg aacggtttc gctgttaaa 
      gtaacacag ccgcttgat gacgaaacc gtacaaaat 
      aacaacgaa atctttgtg gtttttgat gccacagtt 
      gaccgtgct gtggctcac acttcggcc actttggtc 
      ctcaaaatg cccactgaa ctgaaccaa atcgtgccg 
      aaattttcc gaggcattt tacaacgcc cagttggac 
      gaaaagact gtcacgata aaatcaccg atcactatc 
      accagtgac aaagccggc gatacgcaa gttgaagtg 
      attgacgat tacaaggaa tattttgga gcggaattt 
      gatggtacg agttggcag ttgacagtg gttaaggaa 
      ctagaaaca gctctgacg gaaattgtt gtaacgctg 
      gaagcgtcc ttgccaggt gatgacatt aaaacaacc 
      accactgtg attatggct ctgccagat cacggttta 
      acaccaagt cctaagttt acggtacct ctctatgag 
      gctgaatat gtggacgat gaaccgccc tctcttaaa 
      atcactgat ccgatcaca ctccaagcc gggtttaaa 
      gatgctaca gtaactgtt ggttctaat gattatcaa 
      cagtacttc aaacttgcg caaaacaag gacaatgcg 
      cttcagtgg gatatcagc ttaagtaaa ccactaact 
      gacgatatt cttaaaaac aacgcgcaa cttgtcgta 
      actctgaag gccgatttg gaaaattcg gaaggtcca 
      gagtttgcg acactagtc atcaaactg cctgcgcaa 
      tctcaggaa actgtatct tttggtgaa agttactac 
      actgccgat tacactgtg gaaaaggac aaagacaaa 
      ataaattcc tctgaaatt actgtaagt gaaggcgcc 
      acagtcaca gtgattaaa gaatacacc gactacttt 
      ggggtcagt tgtgccgac aaaaagtgt gtcttgaca 
      gtgaaaaaa aatcttgaa gatgatgtt ttaaacacg 
      aaaaatgaa attatcgtt gtattgcaa gccacaaag 
      acagatgtt gccgaaaaa ccgacagtg ccagttatt 
      gtgagtttg cccttgcaa gactctatc gaagcaccg 
      aaattcgaa caaattgtc tacactgct gattatgat 
      aaggaaagt agcacaata tcggcgaat tcgatcaaa 
      tttaaagac actgagact aaaggcata acagtggca 
      attgatcct acgtcagac tataagaat aatttcaaa 
      gtgacttct aaaacggac cattgggaa gttgaagtc 
      agtcaggca ttagatgtc agcggtaaa gtttcgatt 
      tcctttatt ttacaagca acgaaagaa ggtgttcaa 
      acaatgggg gaagctgtc tttgtggtc aatcttcca 
      gcgaatgaa gatacatcg gttgtggaa ttcagtcga 
      gtgacttac accggcaaa tacaatgtc aaggataca 
      accgacgaa gtggttttg gagcaaaat attcaaatc 
      aagggaatt agtgatact agcaaagca aagttaatt 
      atagaaggt gggagtaat aaggacaac tttgagata 
      aaggcatcc gaagagcag aaagaccaa tggattatc 
      ggtgttaaa actgatctt ccgaatgaa attttggat 
      actgaaact tcgatcgtt ctaactttg caagcgaca 
      gtgaatgaa acttcaagt agtggagaa gttggggtt 
      gtaattaat cttccaaag gcatatacc cccacgagt 
      ttttacttc accagtacc tcatacacc gctgactat 
      gaaatcaaa aataactcc cctgaatta aaggtagat 
      tcgaaaatt tcaatcgaa aatgatcca gacagtacg 
      gccacaaca gtgaaactc tcaggagat tttgccaaa 
      tattttgcc gctgattat tccaaaggc atagtaacg 
      ataactcta tcaaaggca attcccaac gaaattgtc 
      gaaaaacag tcagccctt ccggtgaca atcgaagca 
      gcaaaaaaa gacaatcaa gtcgcgaga gcggtcttg 
      gtggtcaga cttcccaag gagacaagc caaaatgac 
      gacgatgac tcgtccgat gaccaaacc ggcctgatt 
      gccgcagtc gcagttttg gccgttttg ttggtcgcc 
      tctctggtc ggaagtgcc gttttctac tacttcaaa 
      ttcaaaact gcaaaaaac tacagcaac atgagtgac 
      agtccgcgg ccttcggaa cgcttcagg aaaaacagt 
      gccgggctt gacacaaaa cataagttt gaacatgcg 
      accagacga ccaacgggg gttatcaag tacccgctc 
      aaagacgac gagagcact gattgcgtg gaagagcca 
      ataagcgag aaacgaaaa tcggtgcag tttgatgtt 
      atacacatc gatgagttg gaaatagag cccgaaaaa 
      accgacgat gaggataac gaaaatgtt tacgaaaat 
      gttgacagt gatagtgac cacaggcgg aactctgag 
      gcctag
    mRNA sequence
    • >TC033254 RA
      gtagtttat tgatggtgg ttattcgga aagtgcgca 
      cagtttctc acaatgaag tttaagaca tttttattt 
      gttatttcg tttgttgtc ccggaatgt ttttcccaa 
      gactctcaa aaatgccaa aatcaggtt atcgataac 
      gacaaaggg ccaaacatt gactggaca aatcaccta 
      ataaaatta gaaaatgaa tctgatgat agacctgtc 
      aatttagta tttaagtat acaggagaa ttaagtgta 
      acgttttct gatgagcct gaatttggt gcaaaatat 
      atagatatc gttgcagaa aataatcaa ctaagcttc 
      aaaagcaac gataactat acaaagatt gaggaagat 
      ccaaacata cagtcttac gttatagga aacacaata 
      agttttcaa agaaaattc actttaaat tgtgatgca 
      gagcaagat attttgaac cttttaata aacatcaaa 
      gatacaaac aaccatgat ccaactttt gccaaaacc 
      agttacagc tacgaaatg cctatgcct ttaatccca 
      aatattgac ttgaccgga tttggcgac gatataatt 
      gtaacagat tacgatttc accaatgac aatgtagca 
      tttactatt gacaatact gctttcgaa gttacaaca 
      aaacacaac aatggttcc aacagttac acggctgtt 
      gtaaaagtt aaaagttat acatatctt tcaaaggac 
      acgcaactg actcttaca gcaactgat tcaggagag 
      ccgccacga tcaagctca accacaatt caaataaaa 
      attgatgaa agcaacagt ttacccgac attccttca 
      gtaccagaa gcttcatat cagtttgac tatgctatt 
      ggtgatgat aaaaaacca gaactgaca ccacttggt 
      gcggacata atagtaacc gccagtgat tcttccaaa 
      cttacagta aaactagaa agtagtgac attgctccc 
      gatgattat tttaccgtc gaacttgac aaaaattca 
      caaaaagta acaataaag cccactgac aaagtcata 
      gaagcttca ccgaacccc gtgctggtt ttaaccctc 
      gtgttaact ctaaacgga aaagaaaaa gacagcagc 
      aaaaccgcg attctggta aacctccca actgagaaa 
      attatcacc ataccacaa ttcggtcaa gcgtactat 
      gaggcccag tatatagtt aacgatgat aaagacacc 
      gtggagctg tcgacagat attacagtt tccgaaaac 
      gctgaggtc acacttgta gatgacgaa aaacacttt 
      agtataagc aaggttggt gataagtgg caagtaact 
      gtgctggcg gatttggat agtagtgac ttgaaaaaa 
      ggtgatatc cccttggaa cttcaagct acagtagtt 
      gttgataaa gagtcatac gttggtttt gctactttg 
      attgttaac cttcctcaa gttgacttg cggtttact 
      aaaccttac tacacggca acttacacc gttagcgag 
      ggggacgcc cttgatacg ttagcagtg gatggagac 
      attgcactg acttcacca agtggtgat gttacagtt 
      gaattagtt gacaatcag gattatttc acattaagt 
      tatattgcg agttctgga acatgtacg gtagaagtt 
      aaggctgat ttagatcaa cctactttg cacaaaggg 
      ggcgatttg atcgtgaat ttcaaagca acactaaac 
      ggtgttgag gaactaggt gctttggtc atcacatta 
      ccggatgtt gttgatgca aaatttgtt gattccaat 
      tatgtggct aattacgta attaccacc gatggagtt 
      acagttaat ataaacggg gctccaatc tcggtaaag 
      ccccccaag ccctccagt caagacatt actgtcagt 
      ttcaaacaa gtgaatgat aattttaaa atcgtgaaa 
      aacgtggac caatatctt atcagtgtt acaactcct 
      ttaaacgcc tcggatttg ctaacgcga gaacctaaa 
      gttttcact ttgcaaaca aacaccgta ccggttgct 
      acaagtact ttggttatt gaattacca gacgttcgg 
      cccaaattc accaaaact tcctatgac gccacttat 
      aaagttgat gctgcagga agtgcaaca gttgaacta 
      aaggacacg attgaatta attacaata ctgaccccg 
      aaactcact tttactggc tcctttgga gaccatttc 
      tcttttgat cccgacaaa gatccacaa attagtgtc 
      acaactaat ctagacgaa gacgttgta aagacaaac 
      ccagaaatt ttactgaca ctacaagca actattgag 
      ggttctagc gagactgaa acgacaatc attgtccta 
      aaattgcca aacgatggg acaacagac acgttgcaa 
      tttgagcaa ccatcgtat gttttttcg tacaaaact 
      ggccaagac aagccttac attgacaac ggggggcaa 
      agtataagt ctagtcaaa gtacacact gacgctgaa 
      attaccatc actgggacg tttagtgaa aatttcgaa 
      ttgtcaggc accgataac caatttagc cttggcatc 
      aaaactccc ttaaaccaa accgtccta gacaccgaa 
      cctgacatt cttctcgtt ctggacgca agtctggcc 
      ggtctggac gatggtttc gccacgatt ttgcttcat 
      ctccctgag aaaaaatcc ccgaatggg ccaaaattc 
      gcccaagcg ttttttaca gccaattat gagttggga 
      gctgaccga cacgataaa gtcacaatt gacggaaag 
      gccataagt ctagctgat ggaacaact gaggcaaac 
      attgaacca gtcggaaca aatagcgaa cactttaag 
      attgagaag aagagtgac ggtagtggt tatgagctg 
      agtttggtt tcgaatttg gatgattcg gttttgatt 
      ggtgaagct gatgtggtt ttgacgttg aaagcaacg 
      cttggtggt gacgttgga gagggaact ttggtggtg 
      aaactgccc aaaaagaca gcaccggct tcgtttaaa 
      caaccaaac tatgtggct aagtacaac atggacact 
      gatccacac acaattgac atggacggg attgaactc 
      gagggtgac tatttggac gacactgtg attactttg 
      aaagtggat gatggactc gaagactat tttacaatt 
      aatgacaaa agtccgtac aaaattgcg ttaactaag 
      aatctggat gatacggta ctgaacagt caacacgaa 
      gttgtagtt actgtagtt gcaacactt gataaaacc 
      tcgaccaca acttccgtg attgtaaca atcccagac 
      ttgccaaca ccaccaaac tttaaggcg agcgtatac 
      tatggcaaa tatgacagt gataagaaa gttgtaacc 
      cttgatgat ccaatcgaa accgatgcc aaagattca 
      agtatcgtt cacatcact gcagaaaag ccctatgac 
      acaaacttt gctgtagag tatgacaaa acgaacggt 
      ttcgctgtt aaagtaaca cagccgctt gatgacgaa 
      accgtacaa aataacaac gaaatcttt gtggttttt 
      gatgccaca gttgaccgt gctgtggct cacacttcg 
      gccactttg gtcctcaaa atgcccact gaactgaac 
      caaatcgtg ccgaaattt tccgaggca ttttacaac 
      gcccagttg gacgaaaag actgtcacg ataaaatca 
      ccgatcact atcaccagt gacaaagcc ggcgatacg 
      caagttgaa gtgattgac gattacaag gaatatttt 
      ggagcggaa tttgatggt acgagttgg cagttgaca 
      gtggttaag gaactagaa acagctctg acggaaatt 
      gttgtaacg ctggaagcg tccttgcca ggtgatgac 
      attaaaaca accaccact gtgattatg gctctgcca 
      gatcacggt ttaacacca agtcctaag tttacggta 
      cctctctat gaggctgaa tatgtggac gatgaaccg 
      ccctctctt aaaatcact gatccgatc acactccaa 
      gccgggttt aaagatgct acagtaact gttggttct 
      aatgattat caacagtac ttcaaactt gcgcaaaac 
      aaggacaat gcgcttcag tgggatatc agcttaagt 
      aaaccacta actgacgat attcttaaa aacaacgcg 
      caacttgtc gtaactctg aaggccgat ttggaaaat 
      tcggaaggt ccagagttt gcgacacta gtcatcaaa 
      ctgcctgcg caatctcag gaaactgta tcttttggt 
      gaaagttac tacactgcc gattacact gtggaaaag 
      gacaaagac aaaataaat tcctctgaa attactgta 
      agtgaaggc gccacagtc acagtgatt aaagaatac 
      accgactac tttggggtc agttgtgcc gacaaaaag 
      tgtgtcttg acagtgaaa aaaaatctt gaagatgat 
      gttttaaac acgaaaaat gaaattatc gttgtattg 
      caagccaca aagacagat gttgccgaa aaaccgaca 
      gtgccagtt attgtgagt ttgcccttg caagactct 
      atcgaagca ccgaaattc gaacaaatt gtctacact 
      gctgattat gataaggaa agtagcaca atatcggcg 
      aattcgatc aaatttaaa gacactgag actaaaggc 
      ataacagtg gcaattgat cctacgtca gactataag 
      aataatttc aaagtgact tctaaaacg gaccattgg 
      gaagttgaa gtcagtcag gcattagat gtcagcggt 
      aaagtttcg atttccttt attttacaa gcaacgaaa 
      gaaggtgtt caaacaatg ggggaagct gtctttgtg 
      gtcaatctt ccagcgaat gaagataca tcggttgtg 
      gaattcagt cgagtgact tacaccggc aaatacaat 
      gtcaaggat acaaccgac gaagtggtt ttggagcaa 
      aatattcaa atcaaggga attagtgat actagcaaa 
      gcaaagtta attatagaa ggtgggagt aataaggac 
      aactttgag ataaaggca tccgaagag cagaaagac 
      caatggatt atcggtgtt aaaactgat cttccgaat 
      gaaattttg gatactgaa acttcgatc gttctaact 
      ttgcaagcg acagtgaat gaaacttca agtagtgga 
      gaagttggg gttgtaatt aatcttcca aaggcatat 
      acccccacg agtttttac ttcaccagt acctcatac 
      accgctgac tatgaaatc aaaaataac tcccctgaa 
      ttaaaggta gattcgaaa atttcaatc gaaaatgat 
      ccagacagt acggccaca acagtgaaa ctctcagga 
      gattttgcc aaatatttt gccgctgat tattccaaa 
      ggcatagta acgataact ctatcaaag gcaattccc 
      aacgaaatt gtcgaaaaa cagtcagcc cttccggtg 
      acaatcgaa gcagcaaaa aaagacaat caagtcgcg 
      agagcggtc ttggtggtc agacttccc aaggagaca 
      agccaaaat gacgacgat gactcgtcc gatgaccaa 
      accggcctg attgccgca gtcgcagtt ttggccgtt 
      ttgttggtc gcctctctg gtcggaagt gccgttttc 
      tactacttc aaattcaaa actgcaaaa aactacagc 
      aacatgagt gacagtccg cggccttcg gaacgcttc 
      aggaaaaac agtgccggg cttgacaca aaacataag 
      tttgaacat gcgaccaga cgaccaacg ggggttatc 
      aagtacccg ctcaaagac gacgagagc actgattgc 
      gtggaagag ccaataagc gagaaacga aaatcggtg 
      cagtttgat gttatacac atcgatgag ttggaaata 
      gagcccgaa aaaaccgac gatgaggat aacgaaaat 
      gtttacgaa aatgttgac agtgatagt gaccacagg 
      cggaactct gaggcctag cccgttgat attatttac 
      acatgcttt aatctgata tttataatt atttaaaat 
      aaagttcaa ctctccaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY