Status
Please wait ...
Gene information
General information
TC013028 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14959538..14971715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013028
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05374

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013028 RA
      MLQITEDTP PDMLAGAMD LTVHLPTGR TVKMSVERS 
      TPMMDLLVQ ITINNHLQL SGYTLQVLG MAEHSDSVL 
      PYKPNTPIG TLDTQHIRV VPKARTAPV PKNIPPGHQ 
      PFESTFRLK VHLPRNQLF VTRVGQSVH LGDIMKKVC 
      EEKILDPLK YEFRHPGNL DEILDPKLT LSDYAITEI 
      YLVQKGTAS LNQAFSSGD IMALRKEEE RKQMHTKTG 
      GGVFNLIFR RGKSSTGSL SSDNRSISP THSDDSRSV 
      TPPAVQQQI ITSQEPPER PKPPQRKRR PAPKPPQEK 
      PKVENGLTI CHSRNSSDS SGYHEASIL SDNNTSLPR 
      RPKSTMIEE SQKTLGVHS HSTSNLSKM SAQSKSTSS 
      LAVPSRKKK SAPPPPPPV LKPDHSDAK HQEPSPVVA 
      SQPCIVAQA DGNGQVANT NPVPTPRTR TKITPHPRP 
      RTIAHENKP QTTEDFKEN EKITAEFCP VSETTDSTV 
      ASEDDLQVV DETREHHDK EEIASEPKT TEYCDIRAE 
      KKETGNELL ETKEHPNKY EDNLQVADE EIAGETPET 
      REHCDELQA DKNGIANEF RETKEREDN PLVVSEEIT 
      SETLETKEH RDIHEDIKG KASELAQLQ KEGVDSFFK 
      SLEKSKAPE IVALHPKPA KSGGSKSIL DSYTRKRGF 
      QIGSSLLDE ENSNQKHSS GLSTPTSVS SALNAFDFV 
      DEIEGLDVE MFTGSLSKN SKNKSVILN ELHGMHELD 
      SLKKSPSRG SLRSVNSLP GFTGTNMKK WSNVEELDE 
      ISLNSHGGK DESVASTAN TSQPLSVQS LPEQKPKEV 
      EEIVEPDWQ YQLPSPPKA FRDSSPVTE SIADSVVTS 
      PELFEKLKA VKDTQSEKE TASDATSMV SEDEKPILN 
      KLSLENLEK RKSLVYNRE LTTTLKEKN DTFSSSLQN 
      FEETYNEVK RSSPKKVIS TSTLPNFKI TTYNNPKQK 
      LDIFEDDTV RSNSDKSSK RNSYAEPTL ERSYVGRSM 
      ENISFRKNS LGRSDDEYK FFKPPEPKG SSVFRSESF 
      SREPMAWVP TKPVARSKS QVALNKYKD TKNLSKMDD 
      ENLTKSSSL FDVSGLQSL GVMRLIQTK LNTPNNSTE 
      QLDHEKSSY QEQPEIKIE VQKPEEKQT TVKQYRPPS 
      INMGTWSER PKVPVNVKD DPDYKSNTS KLIVNTINN 
      DIKSHNSIE VRNKFNNGV SIKVNGVET QTPGNVVIK 
      IGGPKPVFR KPLGNVNEN QRPHSIAFG GDFDISRVP 
      IVRSVEFKK PYKDVQNNN TSVTQINNN NNYKLNGWR 
      QVVEEPPKP PPVESKPVF RVKSFVQNS APVVRGFRG 
      PSSFSTLPG KTAPLNFAV NAPIPFSQK NLRRTESSI 
      TKAPPPAPI MPKTVLGGN GGGDPRGEL LQAIRDFGG 
      KKGLRAAKA
    CDS sequence
    • >TC013028 RA
      atgcttcaa atcaccgaa gatacaccg cctgacatg 
      ctggcaggg gccatggat cttacggta catctaccg 
      accggcaga acagtcaaa atgtcggtg gagcggagc 
      acgcccatg atggacctc ctggtgcaa atcaccatc 
      aacaaccac ctgcaactc tccgggtac accctccag 
      gtgctgggg atggcagaa cacagcgac agcgtccta 
      ccctacaaa ccaaatact cccattgga accctcgat 
      actcagcac attagggtg gtgcccaaa gcgcgcact 
      gccccagta cccaaaaac atcccccct ggacaccag 
      cccttcgaa agcactttc agactaaag gttcacctg 
      cctaggaac caactcttc gtcactcga gtcggacaa 
      agcgtccac ttgggcgac atcatgaag aaagtctgc 
      gaggaaaaa atcctagac ccccttaag tacgagttc 
      aggcatcca ggcaacttg gacgagatt ttggacccc 
      aaactcaca cttagtgat tacgcaatt acggaaata 
      tacctcgta cagaagggg accgcaagt ctgaaccaa 
      gccttctca agtggagac attatggcg ctccggaaa 
      gaagaagaa cgcaagcag atgcacacc aagacggga 
      ggtggagtc ttcaatttg atctttagg agaggaaag 
      tcgagtact ggctcgtta tcgagcgac aatcgttcg 
      atttcgccg actcacagc gacgattcc cgctctgtg 
      actccccca gcggtgcaa caacaaata atcacctcg 
      caagaaccc ccagaacga cccaaaccc ccacaaaga 
      aaacgcaga ccagcgccg aaacccccg caggaaaaa 
      ccaaaagtt gaaaatggc ctaactatt tgtcattcg 
      cgaaacagt agcgatagt tccggttat cacgaagct 
      tcgattctg agtgataac aacacgtcg ttgcctcga 
      aggcccaaa tcaacgatg attgaagaa agtcaaaaa 
      actttaggg gtacacagt cacagtacc agtaattta 
      agtaaaatg agtgcccag tcaaagtca acgtctagt 
      ttagcagtc ccaagtcgt aaaaagaaa tcagcacct 
      cctccgcca ccaccagta ttaaaaccc gaccattcc 
      gacgctaaa caccaagaa cctagtcca gtagttgct 
      agtcaacct tgtatagta gctcaagca gacggtaat 
      gggcaagta gctaacact aatcccgta ccaacaccc 
      agaacacga accaaaatc acaccacat ccacgccca 
      cgtaccatt gcccacgaa aacaaacca caaacaact 
      gaagatttc aaagaaaat gaaaaaata accgcggag 
      ttttgccca gtcagcgaa acaactgac agtactgtt 
      gcatctgaa gatgatctt caggttgtc gatgaaaca 
      agggaacat cacgataaa gaagaaatc gctagtgaa 
      cctaaaaca actgaatat tgcgacata cgtgccgag 
      aaaaaagaa actggtaat gagctgctt gaaacaaaa 
      gaacatccc aacaaatat gaagataat cttcaggtt 
      gccgatgaa gaaatcgct ggtgaaaca cctgaaaca 
      agggaacat tgcgatgaa ttacaggcc gataaaaac 
      ggaatcgct aatgagttc cgtgaaaca aaagaacgt 
      gaagataat cctctggtt gttagtgaa gaaatcact 
      agtgaaaca cttgaaaca aaggaacat cgcgatata 
      catgaagat atcaagggc aaggcgtcc gaattggcg 
      cagttgcag aaggaagga gttgatagt tttttcaag 
      tctttggaa aaatccaaa gctcccgaa atcgtggcc 
      ttgcatcca aaaccggcc aagtcgggc ggctccaag 
      tcgattttg gattcctac acccggaaa agaggtttc 
      cagatcggt agttcgctc ctggatgag gaaaattcg 
      aaccagaaa cactctagt ggtttatcg acaccgacg 
      tccgtgtct tctgccttg aatgcgttt gatttcgtc 
      gatgagatt gaaggactg gacgtggag atgttcact 
      gggtcactt tcgaaaaac tccaaaaat aaaagtgtg 
      attttgaac gagttgcac ggaatgcac gagttggac 
      agtttgaag aagagtccg tcaaggggt agtttacgc 
      agtgtgaac agtcttcca ggttttacg ggtaccaac 
      atgaagaag tggagtaat gtcgaggag cttgatgag 
      atttcgctg aattcacat ggggggaaa gacgaatct 
      gtagcttcg acggccaac acgtcgcaa ccgctctcc 
      gtgcaatcg ctccccgaa cagaagccg aaggaagtc 
      gaggaaatt gtcgaacct gattggcag tatcagctg 
      ccctcaccg ccgaaggcg tttcgcgat tcgagtcct 
      gtgaccgaa tcaatcgcc gattcggtc gtgacctcc 
      cccgaactt tttgaaaaa ctcaaagct gtcaaagac 
      acccaatca gagaaagag actgcaagt gacgccacc 
      agcatggtg agcgaggac gaaaaaccc attctgaat 
      aaactctcg cttgagaat ctcgaaaag cgcaaatcg 
      ttggtctac aaccgcgaa ctgaccact actttaaaa 
      gagaaaaat gatacgttt tcgagcagt ttgcagaat 
      tttgaagag acgtataat gaagtgaag agatctagt 
      ccgaaaaaa gtgatatcc acgagcact ttgcccaat 
      ttcaaaatc acaacctac aacaacccg aaacagaag 
      ttggatatt tttgaggat gatacagtg aggagtaac 
      tcggataag agtagtaaa aggaactcg tacgccgag 
      cccactctg gagaggagt tatgtgggg aggtctatg 
      gagaatatt tccttcagg aaaaatagt ctaggccga 
      tcggatgat gaatacaag ttttttaaa ccgccggaa 
      cccaaaggt tccagtgtt ttccgctca gagtcattc 
      tcaagggaa ccaatggca tgggtacca accaaacct 
      gtggctagg tccaagtca caagtcgcg ttaaacaaa 
      tacaaagac actaaaaat ttatcgaag atggatgac 
      gagaacctg accaagagt agcagtttg tttgatgtc 
      tccggcctt cagagtctt ggggtgatg agattaata 
      caaaccaag ctaaacact ccgaataat tcgaccgaa 
      cagttggat cacgaaaaa tcaagttac caggagcaa 
      cccgaaatc aagatagaa gtacaaaaa cctgaagaa 
      aaacaaacg actgtcaag caataccgg ccgccttcg 
      atcaacatg ggaacgtgg tcagaaaga ccaaaagtc 
      ccagtgaac gtcaaagac gaccccgat tacaaatca 
      aacacttca aaactgatc gtaaacaca atcaacaac 
      gacataaaa agccacaac agcatcgaa gtacgaaac 
      aaatttaac aacggagtt agcattaaa gtgaacgga 
      gtggagaca caaaccccc ggaaacgtt gtaattaaa 
      atcgggggc cctaagcct gtctttcgt aaaccttta 
      gggaacgtt aacgagaat caaagaccg cattctata 
      gctttcggg ggcgatttc gacatatca agggtgcca 
      atcgtcagg tccgttgag tttaaaaaa ccgtacaaa 
      gacgtgcaa aacaacaac acctccgtc actcaaatt 
      aacaacaat aataattac aaattgaac ggttggagg 
      caagtggtg gaagagccc cccaagccg ccgccagtc 
      gagtccaaa cctgtcttc cgggtgaag agtttcgtc 
      cagaattcg gcccccgtt gtgcggggc ttccgaggc 
      ccctccagt ttttccact cttccgggc aagacagct 
      cctttgaat ttcgcagtg aatgctccc atccccttc 
      tcgcagaag aacctgcga aggactgag tctagcatc 
      acgaaggcg ccgccccca gccccgatt atgcccaag 
      actgtgttg gggggcaac ggtgggggg gacccccgg 
      ggtgagctg ctgcaagcc attcgagat tttgggggg 
      aagaagggg ctgcgggcc gctaaagcc tga
    mRNA sequence
    • >TC013028 RA
      catcatcat catcgcacc aaatagaac cttgtcagg 
      aattgctcg ataacattg aaaccgata agcgattct 
      ctcgattgt ggccggaat tcttgcacc tgctgattg 
      gattcgcgg cctcttcgc tcatcagga gagcgagtg 
      agtggtcga agacaggcc tccgagacc ccactttca 
      gtcgatttc gcgacaatg tggcgtaag gtaacaggt 
      gctgcttag tgcatgtgc gttggtctt atcacagtg 
      ttgtcaatt gaactgata acgatattt actactgga 
      gaactcagt tgtgtttat gtggactat gtaaacgtt 
      ttctcacgg attatcctc gatcgatca gccggtggc 
      gcagcgacc atgcttcaa atcaccgaa gatacaccg 
      cctgacatg ctggcaggg gccatggat cttacggta 
      catctaccg accggcaga acagtcaaa atgtcggtg 
      gagcggagc acgcccatg atggacctc ctggtgcaa 
      atcaccatc aacaaccac ctgcaactc tccgggtac 
      accctccag gtgctgggg atggcagaa cacagcgac 
      agcgtccta ccctacaaa ccaaatact cccattgga 
      accctcgat actcagcac attagggtg gtgcccaaa 
      gcgcgcact gccccagta cccaaaaac atcccccct 
      ggacaccag cccttcgaa agcactttc agactaaag 
      gttcacctg cctaggaac caactcttc gtcactcga 
      gtcggacaa agcgtccac ttgggcgac atcatgaag 
      aaagtctgc gaggaaaaa atcctagac ccccttaag 
      tacgagttc aggcatcca ggcaacttg gacgagatt 
      ttggacccc aaactcaca cttagtgat tacgcaatt 
      acggaaata tacctcgta cagaagggg accgcaagt 
      ctgaaccaa gccttctca agtggagac attatggcg 
      ctccggaaa gaagaagaa cgcaagcag atgcacacc 
      aagacggga ggtggagtc ttcaatttg atctttagg 
      agaggaaag tcgagtact ggctcgtta tcgagcgac 
      aatcgttcg atttcgccg actcacagc gacgattcc 
      cgctctgtg actccccca gcggtgcaa caacaaata 
      atcacctcg caagaaccc ccagaacga cccaaaccc 
      ccacaaaga aaacgcaga ccagcgccg aaacccccg 
      caggaaaaa ccaaaagtt gaaaatggc ctaactatt 
      tgtcattcg cgaaacagt agcgatagt tccggttat 
      cacgaagct tcgattctg agtgataac aacacgtcg 
      ttgcctcga aggcccaaa tcaacgatg attgaagaa 
      agtcaaaaa actttaggg gtacacagt cacagtacc 
      agtaattta agtaaaatg agtgcccag tcaaagtca 
      acgtctagt ttagcagtc ccaagtcgt aaaaagaaa 
      tcagcacct cctccgcca ccaccagta ttaaaaccc 
      gaccattcc gacgctaaa caccaagaa cctagtcca 
      gtagttgct agtcaacct tgtatagta gctcaagca 
      gacggtaat gggcaagta gctaacact aatcccgta 
      ccaacaccc agaacacga accaaaatc acaccacat 
      ccacgccca cgtaccatt gcccacgaa aacaaacca 
      caaacaact gaagatttc aaagaaaat gaaaaaata 
      accgcggag ttttgccca gtcagcgaa acaactgac 
      agtactgtt gcatctgaa gatgatctt caggttgtc 
      gatgaaaca agggaacat cacgataaa gaagaaatc 
      gctagtgaa cctaaaaca actgaatat tgcgacata 
      cgtgccgag aaaaaagaa actggtaat gagctgctt 
      gaaacaaaa gaacatccc aacaaatat gaagataat 
      cttcaggtt gccgatgaa gaaatcgct ggtgaaaca 
      cctgaaaca agggaacat tgcgatgaa ttacaggcc 
      gataaaaac ggaatcgct aatgagttc cgtgaaaca 
      aaagaacgt gaagataat cctctggtt gttagtgaa 
      gaaatcact agtgaaaca cttgaaaca aaggaacat 
      cgcgatata catgaagat atcaagggc aaggcgtcc 
      gaattggcg cagttgcag aaggaagga gttgatagt 
      tttttcaag tctttggaa aaatccaaa gctcccgaa 
      atcgtggcc ttgcatcca aaaccggcc aagtcgggc 
      ggctccaag tcgattttg gattcctac acccggaaa 
      agaggtttc cagatcggt agttcgctc ctggatgag 
      gaaaattcg aaccagaaa cactctagt ggtttatcg 
      acaccgacg tccgtgtct tctgccttg aatgcgttt 
      gatttcgtc gatgagatt gaaggactg gacgtggag 
      atgttcact gggtcactt tcgaaaaac tccaaaaat 
      aaaagtgtg attttgaac gagttgcac ggaatgcac 
      gagttggac agtttgaag aagagtccg tcaaggggt 
      agtttacgc agtgtgaac agtcttcca ggttttacg 
      ggtaccaac atgaagaag tggagtaat gtcgaggag 
      cttgatgag atttcgctg aattcacat ggggggaaa 
      gacgaatct gtagcttcg acggccaac acgtcgcaa 
      ccgctctcc gtgcaatcg ctccccgaa cagaagccg 
      aaggaagtc gaggaaatt gtcgaacct gattggcag 
      tatcagctg ccctcaccg ccgaaggcg tttcgcgat 
      tcgagtcct gtgaccgaa tcaatcgcc gattcggtc 
      gtgacctcc cccgaactt tttgaaaaa ctcaaagct 
      gtcaaagac acccaatca gagaaagag actgcaagt 
      gacgccacc agcatggtg agcgaggac gaaaaaccc 
      attctgaat aaactctcg cttgagaat ctcgaaaag 
      cgcaaatcg ttggtctac aaccgcgaa ctgaccact 
      actttaaaa gagaaaaat gatacgttt tcgagcagt 
      ttgcagaat tttgaagag acgtataat gaagtgaag 
      agatctagt ccgaaaaaa gtgatatcc acgagcact 
      ttgcccaat ttcaaaatc acaacctac aacaacccg 
      aaacagaag ttggatatt tttgaggat gatacagtg 
      aggagtaac tcggataag agtagtaaa aggaactcg 
      tacgccgag cccactctg gagaggagt tatgtgggg 
      aggtctatg gagaatatt tccttcagg aaaaatagt 
      ctaggccga tcggatgat gaatacaag ttttttaaa 
      ccgccggaa cccaaaggt tccagtgtt ttccgctca 
      gagtcattc tcaagggaa ccaatggca tgggtacca 
      accaaacct gtggctagg tccaagtca caagtcgcg 
      ttaaacaaa tacaaagac actaaaaat ttatcgaag 
      atggatgac gagaacctg accaagagt agcagtttg 
      tttgatgtc tccggcctt cagagtctt ggggtgatg 
      agattaata caaaccaag ctaaacact ccgaataat 
      tcgaccgaa cagttggat cacgaaaaa tcaagttac 
      caggagcaa cccgaaatc aagatagaa gtacaaaaa 
      cctgaagaa aaacaaacg actgtcaag caataccgg 
      ccgccttcg atcaacatg ggaacgtgg tcagaaaga 
      ccaaaagtc ccagtgaac gtcaaagac gaccccgat 
      tacaaatca aacacttca aaactgatc gtaaacaca 
      atcaacaac gacataaaa agccacaac agcatcgaa 
      gtacgaaac aaatttaac aacggagtt agcattaaa 
      gtgaacgga gtggagaca caaaccccc ggaaacgtt 
      gtaattaaa atcgggggc cctaagcct gtctttcgt 
      aaaccttta gggaacgtt aacgagaat caaagaccg 
      cattctata gctttcggg ggcgatttc gacatatca 
      agggtgcca atcgtcagg tccgttgag tttaaaaaa 
      ccgtacaaa gacgtgcaa aacaacaac acctccgtc 
      actcaaatt aacaacaat aataattac aaattgaac 
      ggttggagg caagtggtg gaagagccc cccaagccg 
      ccgccagtc gagtccaaa cctgtcttc cgggtgaag 
      agtttcgtc cagaattcg gcccccgtt gtgcggggc 
      ttccgaggc ccctccagt ttttccact cttccgggc 
      aagacagct cctttgaat ttcgcagtg aatgctccc 
      atccccttc tcgcagaag aacctgcga aggactgag 
      tctagcatc acgaaggcg ccgccccca gccccgatt 
      atgcccaag actgtgttg gggggcaac ggtgggggg 
      gacccccgg ggtgagctg ctgcaagcc attcgagat 
      tttgggggg aagaagggg ctgcgggcc gctaaagcc 
      tgatgtata tagtcatat tcgtaataa aaagctcaa 
      tatgttctg aattattaa tcagatact atagggacc 
      aaaggtttt gtggaacat gattattta tttatgttt 
      attactcat gtatgtata gtgaaataa attatgaat 
      aaagtgtgt aacaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY