Status
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Gene information
General information
TC033264
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013018
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15107423..15112826
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033264
    iBeetle screen iB_05373

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033264 RA
      MRTSLLCLE YVFILALCS RGLVCEDAA PTPPPSAPI 
      GENVSSDSI QDSKKTIES EKSDDVIRG LEPSADVVS 
      NFNEKTEDT LQVFAEATS QNSGVANDL TTSGQPVVV 
      TLSDVATAE LQQRAILPS QDTPPEPQS TNHTFKLNQ 
      SRSDLPTVK DNLTEEIPS FSEWAQKQL QEAEKNKSN 
      TSTHHPNGN KQASGAKLR WKNYASLDC GAKVVASNP 
      EAVSPSAIL SPSRDEYKL NTCTSRIWF IVELCEAIQ 
      AKKIDLANF ELFSSSPKD FAVFVSDRF PTREWSNVG 
      HFTAKDERD VQSFDLHPH LFGKYIKVE VKSHYGSEH 
      YCPISLFRV YGTSEFEVL QKEDQAHED RGDDDDDDD 
      GSLDLENGD ARKNLFSSA TDAVISMVK KAAEVLGNK 
      GNYSNQTEI LNNTKQAVP LVRVCTSPS HLVVCDNCS 
      DALFGRVYE LLSCHDSQI WGLINLAFI RNVLVESSL 
      CQKFGFCKV SDKFEAHAK YIEALFPEY LLGAMCNMA 
      AVYQNKVVL NVSNHSNDT IIDDDQMIN IETSEPHVL 
      PLETTRKEP EVTDALSLQ KSEAVSTPS LPIVTLTSQ 
      IKPTKTLNS ESDSRQSNT EASSVRSEI KSIVTSTEP 
      VQSNLTEPE EPEVENVTE SVELDDPLD AATPQAQKE 
      SVFLRLSNR IKALERNMS LSGQYLEEL SKRYKKQVE 
      EIQKLLDKT ILSLNEESQ KKDERNKQL EERLNVLTS 
      NLEALLAER RSWSSTISC VVISSLVTL FIVTFCCKI 
      PEPVAARRP ELKRRKSID VVQHTAPKK KRRPSDQAL 
      KIVRSSMEA SDNLRKKKK RKNMLRRSN SISTLGGEE 
      KKAWPEAGS IDWVEGKRF EEVPFVLEE SEHSTLEPI 
      ALEEKIAPP SFVQTAVMA RAVRVNGEA KREENGGVA 
      VEGTPKKEK KGLKRIFRK VF
    • >TC033264 RB
      MRTSLLCLE YVFILALCR GLVCEDAAP TPPPSAPIG 
      ENVSSDSIQ DSKKTIESE KSDDVIRGL EPSADVVSN 
      FNEKTEDTL QVFAEATSQ NSGVANDLT TSGQPVVVT 
      LSDVATAEL QQRAILPSQ DTPPEPQST NHTFKLNQS 
      RSDLPTVKD NLTEEIPSF SEWAQKQLQ EAEKNKSNT 
      STHHPNGNK QASGAKLRW KNYASLDCG AKVVASNPE 
      AVSPSAILS PSRDEYKLN TCTSRIWFI VELCEAIQA 
      KKIDLANFE LFSSSPKDF AVFVSDRFP TREWSNVGH 
      FTAKDERDV QSFDLHPHL FGKYIKVEV KSHYGSEHY 
      CPISLFRVY GTSEFEVLQ KEDQAHEDR GDDDDDDDG 
      SLDLENGDA RKNLFSSAT DAVISMVKK AAEVLGNKG 
      NYSNQTEIL NNTKQAVPL VRVCTSPSH LVVCDNCSD 
      ALFGRVYEL LSCHDSQIW GLINLAFIR NVLVESSLC 
      QKFGFCKVS DKFEAHAKY IEALFPEYL LGAMCNMAA 
      VYQNKVVLN VSNHSNDTI IDDDQMINI ETSEPHVLP 
      LETTRKEPE VTDALSLQK SEAVSTPSL PIVTLTSQI 
      KPTKTLNSE SDSRQSNTE ASSVRSEIK SIVTSTEPV 
      QSNLTEPEE PEVENVTES VELDDPLDA ATPQAQKES 
      VFLRLSNRI KALERNMSL SGQYLEELS KRYKKQVEE 
      IQKLLDKTI LSLNEESQK KDERNKQLE ERLNVLTSN 
      LEALLAERR SWSSTISCV VISSLVTLF IVTFCCKIP 
      EPVAARRPE LKRRKSIDV VQHTAPKKK RRPSDQALK 
      IVRSSMEAS DNLRKKKKR KNMLRRSNS ISTLGGEEK 
      KAWPEAGSI DWVEGKRFE EVPFVLEES EHSTLEPIA 
      LEEKIAPPS FVQTAVMAR AVRVNGEAK REENGGVAV 
      EGTPKKEKK GLKRIFRKV F
    CDS sequence
    • >TC033264 RA
      atgaggact tcgttacta tgtctcgag tatgttttc 
      attttggct ttgtgcagt agaggtctc gtgtgcgaa 
      gatgcggcg ccgacgcca ccacccagt gccccaatc 
      ggtgaaaat gtttcatca gacagtatt caagacagc 
      aaaaaaacg attgaaagt gaaaaaagt gacgatgta 
      attcgcggt ttagaacca agtgcggac gttgtgagt 
      aatttcaat gaaaagacc gaagacact ttacaagtg 
      ttcgccgaa gcgacttcc cagaattca ggggttgcc 
      aatgatcta acaacaagc gggcagccg gtggttgtg 
      acactttcg gacgtcgcc accgccgag ctccaacaa 
      cgcgccata cttcctagt caagacaca ccccctgag 
      ccccaatcc accaatcac accttcaaa ctcaaccaa 
      tcaagatct gatttaccc actgttaaa gacaaccta 
      actgaagaa attccctcg ttttctgaa tgggcgcag 
      aagcaactt caagaagcc gaaaaaaac aaatccaac 
      acctccaca catcacccc aacggcaac aaacaagcc 
      tcaggggcg aagctccgc tggaaaaac tacgcatcg 
      ttggattgt ggcgccaaa gtggtggct tccaatcca 
      gaggctgtg agccctagc gctatcttg agcccctca 
      cgtgatgag tacaaactc aacacttgt acaagtcgt 
      atttggttt attgtcgaa ttatgcgaa gcaattcaa 
      gccaaaaaa atcgatttg gctaatttt gaattattt 
      tcttcgtcg ccgaaagat tttgcggtt ttcgtaagc 
      gatcggttt ccgacacgt gaatggtca aatgttggc 
      catttcacg gcgaaggat gaacgcgac gtgcaaagt 
      ttcgacctt catccgcac ctcttcggg aaatatatc 
      aaagtcgag gtgaaaagc cactacggt tcggaacac 
      tattgtccg atttcgctg tttcgcgtt tatgggact 
      tcggaattt gaggttttg caaaaggag gatcaagca 
      cacgaggat cgaggtgat gatgatgac gatgatgac 
      ggctcactt gatttggaa aatggtgat gcgcggaag 
      aatttattc agttcggcg acggatgct gtcatttcg 
      atggtgaaa aaagcggct gaggttttg ggaaataag 
      gggaattac agcaatcag actgaaatt ttaaataac 
      accaagcag gccgtgcct ttggttcgg gtgtgtacg 
      tcaccgagt catctagtc gtttgtgat aactgcagc 
      gatgccctc tttgggcgt gtttatgaa ctattgagt 
      tgtcacgac tcacaaatt tggggttta attaattta 
      gcgtttatt cgcaatgtg ctggttgag tcaagctta 
      tgccaaaaa tttggtttt tgtaaagtt agcgataag 
      tttgaggcg catgccaaa tatattgaa gcgctattt 
      ccggaatat ttgctaggc gccatgtgc aacatggcc 
      gccgtttac caaaacaaa gtcgtactt aacgtaagt 
      aatcattcg aatgatacg atcattgat gatgatcaa 
      atgataaat atcgaaacg tcggaacca catgtactt 
      cccctcgag acgacgcga aaagaaccg gaagttacc 
      gatgccttg tcattgcaa aaatcggaa gccgtgtcg 
      acgccatct ttaccaatt gtcaccctg acgtcacaa 
      atcaagcct actaaaact ttgaattcg gaatcggat 
      tcaagacaa agtaatact gaggcgagt tcagtccga 
      agtgagatt aagtcaatt gtgacgtca actgaaccg 
      gtccagtct aacttaacc gaaccggag gaaccagaa 
      gtggaaaat gtgactgaa agtgtggaa ttagatgac 
      ccacttgat gcggcgaca ccacaggct cagaaagaa 
      tcggttttt ttgcgcctt tctaaccga attaaagcc 
      ctggagcgc aacatgtcc ctctcaggc caatacctc 
      gaagagttg agtaaacga tacaagaaa caagtcgag 
      gagatacaa aaactcctc gacaaaaca atactcagt 
      ttaaacgaa gaaagtcag aagaaagac gaaaggaac 
      aaacagttg gaggagcga cttaacgtg ctaacctca 
      aatttagag gcccttttg gccgaacgc aggagctgg 
      tcctcgacc atttcctgc gtcgtcatt tcctctcta 
      gtcacttta tttattgtt actttttgc tgtaaaatc 
      cccgaaccg gtggcggcg agacggccc gagttaaaa 
      cgacgaaaa tcgattgat gttgtacaa cacaccgcc 
      cccaagaaa aaacggcgt cccagtgac caagcttta 
      aagatcgtc cggtcgtca atggaagct agtgataat 
      ttgaggaag aaaaagaag aggaagaat atgctgagg 
      cgatcgaat tcgattagt acgcttggg ggggaggag 
      aaaaaggcg tggcctgag gcggggtct attgactgg 
      gtggagggg aagcggttt gaagaagtg ccgtttgtg 
      ttggaggaa agtgagcac tcgacattg gaaccaatc 
      gcattggaa gagaaaatc gcgccgcct agttttgtg 
      caaacggcg gtgatggcg agggcggtg cgagtcaac 
      ggcgaggcg aagagggag gagaatggg ggcgtggca 
      gttgaagga acgcccaag aaggagaag aaggggttg 
      aagaggatt tttaggaag gtgttttaa
    • >TC033264 RB
      atgaggact tcgttacta tgtctcgag tatgttttc 
      attttggct ttgtgcaga ggtctcgtg tgcgaagat 
      gcggcgccg acgccacca cccagtgcc ccaatcggt 
      gaaaatgtt tcatcagac agtattcaa gacagcaaa 
      aaaacgatt gaaagtgaa aaaagtgac gatgtaatt 
      cgcggttta gaaccaagt gcggacgtt gtgagtaat 
      ttcaatgaa aagaccgaa gacacttta caagtgttc 
      gccgaagcg acttcccag aattcaggg gttgccaat 
      gatctaaca acaagcggg cagccggtg gttgtgaca 
      ctttcggac gtcgccacc gccgagctc caacaacgc 
      gccatactt cctagtcaa gacacaccc cctgagccc 
      caatccacc aatcacacc ttcaaactc aaccaatca 
      agatctgat ttacccact gttaaagac aacctaact 
      gaagaaatt ccctcgttt tctgaatgg gcgcagaag 
      caacttcaa gaagccgaa aaaaacaaa tccaacacc 
      tccacacat caccccaac ggcaacaaa caagcctca 
      ggggcgaag ctccgctgg aaaaactac gcatcgttg 
      gattgtggc gccaaagtg gtggcttcc aatccagag 
      gctgtgagc cctagcgct atcttgagc ccctcacgt 
      gatgagtac aaactcaac acttgtaca agtcgtatt 
      tggtttatt gtcgaatta tgcgaagca attcaagcc 
      aaaaaaatc gatttggct aattttgaa ttattttct 
      tcgtcgccg aaagatttt gcggttttc gtaagcgat 
      cggtttccg acacgtgaa tggtcaaat gttggccat 
      ttcacggcg aaggatgaa cgcgacgtg caaagtttc 
      gaccttcat ccgcacctc ttcgggaaa tatatcaaa 
      gtcgaggtg aaaagccac tacggttcg gaacactat 
      tgtccgatt tcgctgttt cgcgtttat gggacttcg 
      gaatttgag gttttgcaa aaggaggat caagcacac 
      gaggatcga ggtgatgat gatgacgat gatgacggc 
      tcacttgat ttggaaaat ggtgatgcg cggaagaat 
      ttattcagt tcggcgacg gatgctgtc atttcgatg 
      gtgaaaaaa gcggctgag gttttggga aataagggg 
      aattacagc aatcagact gaaatttta aataacacc 
      aagcaggcc gtgcctttg gttcgggtg tgtacgtca 
      ccgagtcat ctagtcgtt tgtgataac tgcagcgat 
      gccctcttt gggcgtgtt tatgaacta ttgagttgt 
      cacgactca caaatttgg ggtttaatt aatttagcg 
      tttattcgc aatgtgctg gttgagtca agcttatgc 
      caaaaattt ggtttttgt aaagttagc gataagttt 
      gaggcgcat gccaaatat attgaagcg ctatttccg 
      gaatatttg ctaggcgcc atgtgcaac atggccgcc 
      gtttaccaa aacaaagtc gtacttaac gtaagtaat 
      cattcgaat gatacgatc attgatgat gatcaaatg 
      ataaatatc gaaacgtcg gaaccacat gtacttccc 
      ctcgagacg acgcgaaaa gaaccggaa gttaccgat 
      gccttgtca ttgcaaaaa tcggaagcc gtgtcgacg 
      ccatcttta ccaattgtc accctgacg tcacaaatc 
      aagcctact aaaactttg aattcggaa tcggattca 
      agacaaagt aatactgag gcgagttca gtccgaagt 
      gagattaag tcaattgtg acgtcaact gaaccggtc 
      cagtctaac ttaaccgaa ccggaggaa ccagaagtg 
      gaaaatgtg actgaaagt gtggaatta gatgaccca 
      cttgatgcg gcgacacca caggctcag aaagaatcg 
      gtttttttg cgcctttct aaccgaatt aaagccctg 
      gagcgcaac atgtccctc tcaggccaa tacctcgaa 
      gagttgagt aaacgatac aagaaacaa gtcgaggag 
      atacaaaaa ctcctcgac aaaacaata ctcagttta 
      aacgaagaa agtcagaag aaagacgaa aggaacaaa 
      cagttggag gagcgactt aacgtgcta acctcaaat 
      ttagaggcc cttttggcc gaacgcagg agctggtcc 
      tcgaccatt tcctgcgtc gtcatttcc tctctagtc 
      actttattt attgttact ttttgctgt aaaatcccc 
      gaaccggtg gcggcgaga cggcccgag ttaaaacga 
      cgaaaatcg attgatgtt gtacaacac accgccccc 
      aagaaaaaa cggcgtccc agtgaccaa gctttaaag 
      atcgtccgg tcgtcaatg gaagctagt gataatttg 
      aggaagaaa aagaagagg aagaatatg ctgaggcga 
      tcgaattcg attagtacg cttgggggg gaggagaaa 
      aaggcgtgg cctgaggcg gggtctatt gactgggtg 
      gaggggaag cggtttgaa gaagtgccg tttgtgttg 
      gaggaaagt gagcactcg acattggaa ccaatcgca 
      ttggaagag aaaatcgcg ccgcctagt tttgtgcaa 
      acggcggtg atggcgagg gcggtgcga gtcaacggc 
      gaggcgaag agggaggag aatgggggc gtggcagtt 
      gaaggaacg cccaagaag gagaagaag gggttgaag 
      aggattttt aggaaggtg ttttaa
    mRNA sequence
    • >TC033264 RA
      atagtaaaa agtgcgtga cgaaatgca tttggaggt 
      ttcattgtt gcacttgtg ataggagga caaaattgt 
      tgccaacct gatgattac gacgctccc actcttgtc 
      aaaccgtta ccaacagta aaagtacac gtcagttgt 
      ttaattcgt atcagttag tttattctt gattagtgt 
      tcgattgta gcaaaacca tgtgcttaa aacccgtat 
      gtaacactt gcataaaat gaggacttc gttactatg 
      tctcgagta tgttttcat tttggcttt gtgcagtag 
      aggtctcgt gtgcgaaga tgcggcgcc gacgccacc 
      acccagtgc cccaatcgg tgaaaatgt ttcatcaga 
      cagtattca agacagcaa aaaaacgat tgaaagtga 
      aaaaagtga cgatgtaat tcgcggttt agaaccaag 
      tgcggacgt tgtgagtaa tttcaatga aaagaccga 
      agacacttt acaagtgtt cgccgaagc gacttccca 
      gaattcagg ggttgccaa tgatctaac aacaagcgg 
      gcagccggt ggttgtgac actttcgga cgtcgccac 
      cgccgagct ccaacaacg cgccatact tcctagtca 
      agacacacc ccctgagcc ccaatccac caatcacac 
      cttcaaact caaccaatc aagatctga tttacccac 
      tgttaaaga caacctaac tgaagaaat tccctcgtt 
      ttctgaatg ggcgcagaa gcaacttca agaagccga 
      aaaaaacaa atccaacac ctccacaca tcaccccaa 
      cggcaacaa acaagcctc aggggcgaa gctccgctg 
      gaaaaacta cgcatcgtt ggattgtgg cgccaaagt 
      ggtggcttc caatccaga ggctgtgag ccctagcgc 
      tatcttgag cccctcacg tgatgagta caaactcaa 
      cacttgtac aagtcgtat ttggtttat tgtcgaatt 
      atgcgaagc aattcaagc caaaaaaat cgatttggc 
      taattttga attattttc ttcgtcgcc gaaagattt 
      tgcggtttt cgtaagcga tcggtttcc gacacgtga 
      atggtcaaa tgttggcca tttcacggc gaaggatga 
      acgcgacgt gcaaagttt cgaccttca tccgcacct 
      cttcgggaa atatatcaa agtcgaggt gaaaagcca 
      ctacggttc ggaacacta ttgtccgat ttcgctgtt 
      tcgcgttta tgggacttc ggaatttga ggttttgca 
      aaaggagga tcaagcaca cgaggatcg aggtgatga 
      tgatgacga tgatgacgg ctcacttga tttggaaaa 
      tggtgatgc gcggaagaa tttattcag ttcggcgac 
      ggatgctgt catttcgat ggtgaaaaa agcggctga 
      ggttttggg aaataaggg gaattacag caatcagac 
      tgaaatttt aaataacac caagcaggc cgtgccttt 
      ggttcgggt gtgtacgtc accgagtca tctagtcgt 
      ttgtgataa ctgcagcga tgccctctt tgggcgtgt 
      ttatgaact attgagttg tcacgactc acaaatttg 
      gggtttaat taatttagc gtttattcg caatgtgct 
      ggttgagtc aagcttatg ccaaaaatt tggtttttg 
      taaagttag cgataagtt tgaggcgca tgccaaata 
      tattgaagc gctatttcc ggaatattt gctaggcgc 
      catgtgcaa catggccgc cgtttacca aaacaaagt 
      cgtacttaa cgtaagtaa tcattcgaa tgatacgat 
      cattgatga tgatcaaat gataaatat cgaaacgtc 
      ggaaccaca tgtacttcc cctcgagac gacgcgaaa 
      agaaccgga agttaccga tgccttgtc attgcaaaa 
      atcggaagc cgtgtcgac gccatcttt accaattgt 
      caccctgac gtcacaaat caagcctac taaaacttt 
      gaattcgga atcggattc aagacaaag taatactga 
      ggcgagttc agtccgaag tgagattaa gtcaattgt 
      gacgtcaac tgaaccggt ccagtctaa cttaaccga 
      accggagga accagaagt ggaaaatgt gactgaaag 
      tgtggaatt agatgaccc acttgatgc ggcgacacc 
      acaggctca gaaagaatc ggttttttt gcgcctttc 
      taaccgaat taaagccct ggagcgcaa catgtccct 
      ctcaggcca atacctcga agagttgag taaacgata 
      caagaaaca agtcgagga gatacaaaa actcctcga 
      caaaacaat actcagttt aaacgaaga aagtcagaa 
      gaaagacga aaggaacaa acagttgga ggagcgact 
      taacgtgct aacctcaaa tttagaggc ccttttggc 
      cgaacgcag gagctggtc ctcgaccat ttcctgcgt 
      cgtcatttc ctctctagt cactttatt tattgttac 
      tttttgctg taaaatccc cgaaccggt ggcggcgag 
      acggcccga gttaaaacg acgaaaatc gattgatgt 
      tgtacaaca caccgcccc caagaaaaa acggcgtcc 
      cagtgacca agctttaaa gatcgtccg gtcgtcaat 
      ggaagctag tgataattt gaggaagaa aaagaagag 
      gaagaatat gctgaggcg atcgaattc gattagtac 
      gcttggggg ggaggagaa aaaggcgtg gcctgaggc 
      ggggtctat tgactgggt ggaggggaa gcggtttga 
      agaagtgcc gtttgtgtt ggaggaaag tgagcactc 
      gacattgga accaatcgc attggaaga gaaaatcgc 
      gccgcctag ttttgtgca aacggcggt gatggcgag 
      ggcggtgcg agtcaacgg cgaggcgaa gagggagga 
      gaatggggg cgtggcagt tgaaggaac gcccaagaa 
      ggagaagaa ggggttgaa gaggatttt taggaaggt 
      gttttaaga ctgattgta ggcgttttt aagttagtt 
      gagttattt ataaaggta atatatttt tgtgtattt 
      atttaggaa ttccttttt gaggcctgt gataaaatt 
      tatttttta aaccgacat cgtagatgt tgcatattt 
      gtaaatttt aatttgcat tgcctgata atagagatc 
      tacaaagta catggggtt ttactgcta actttaatt 
      tcaggatac ccaatttaa ataaaaaaa aaacactta 
      t
    • >TC033264 RB
      atagtaaaa agtgcgtga cgaaatgca tttggaggt 
      ttcattgtt gcacttgtg ataggagga caaaattgt 
      tgccaacct gatgattac gacgctccc actcttgtc 
      aaaccgtta ccaacagta aaagtacac gtcagttgt 
      ttaattcgt atcagttag tttattctt gattagtgt 
      tcgattgta gcaaaacca tgtgcttaa aacccgtat 
      gtaacactt gcataaaat gaggacttc gttactatg 
      tctcgagta tgttttcat tttggcttt gtgcagagg 
      tctcgtgtg cgaagatgc ggcgccgac gccaccacc 
      cagtgcccc aatcggtga aaatgtttc atcagacag 
      tattcaaga cagcaaaaa aacgattga aagtgaaaa 
      aagtgacga tgtaattcg cggtttaga accaagtgc 
      ggacgttgt gagtaattt caatgaaaa gaccgaaga 
      cactttaca agtgttcgc cgaagcgac ttcccagaa 
      ttcaggggt tgccaatga tctaacaac aagcgggca 
      gccggtggt tgtgacact ttcggacgt cgccaccgc 
      cgagctcca acaacgcgc catacttcc tagtcaaga 
      cacaccccc tgagcccca atccaccaa tcacacctt 
      caaactcaa ccaatcaag atctgattt acccactgt 
      taaagacaa cctaactga agaaattcc ctcgttttc 
      tgaatgggc gcagaagca acttcaaga agccgaaaa 
      aaacaaatc caacacctc cacacatca ccccaacgg 
      caacaaaca agcctcagg ggcgaagct ccgctggaa 
      aaactacgc atcgttgga ttgtggcgc caaagtggt 
      ggcttccaa tccagaggc tgtgagccc tagcgctat 
      cttgagccc ctcacgtga tgagtacaa actcaacac 
      ttgtacaag tcgtatttg gtttattgt cgaattatg 
      cgaagcaat tcaagccaa aaaaatcga tttggctaa 
      ttttgaatt attttcttc gtcgccgaa agattttgc 
      ggttttcgt aagcgatcg gtttccgac acgtgaatg 
      gtcaaatgt tggccattt cacggcgaa ggatgaacg 
      cgacgtgca aagtttcga ccttcatcc gcacctctt 
      cgggaaata tatcaaagt cgaggtgaa aagccacta 
      cggttcgga acactattg tccgatttc gctgtttcg 
      cgtttatgg gacttcgga atttgaggt tttgcaaaa 
      ggaggatca agcacacga ggatcgagg tgatgatga 
      tgacgatga tgacggctc acttgattt ggaaaatgg 
      tgatgcgcg gaagaattt attcagttc ggcgacgga 
      tgctgtcat ttcgatggt gaaaaaagc ggctgaggt 
      tttgggaaa taaggggaa ttacagcaa tcagactga 
      aattttaaa taacaccaa gcaggccgt gcctttggt 
      tcgggtgtg tacgtcacc gagtcatct agtcgtttg 
      tgataactg cagcgatgc cctctttgg gcgtgttta 
      tgaactatt gagttgtca cgactcaca aatttgggg 
      tttaattaa tttagcgtt tattcgcaa tgtgctggt 
      tgagtcaag cttatgcca aaaatttgg tttttgtaa 
      agttagcga taagtttga ggcgcatgc caaatatat 
      tgaagcgct atttccgga atatttgct aggcgccat 
      gtgcaacat ggccgccgt ttaccaaaa caaagtcgt 
      acttaacgt aagtaatca ttcgaatga tacgatcat 
      tgatgatga tcaaatgat aaatatcga aacgtcgga 
      accacatgt acttcccct cgagacgac gcgaaaaga 
      accggaagt taccgatgc cttgtcatt gcaaaaatc 
      ggaagccgt gtcgacgcc atctttacc aattgtcac 
      cctgacgtc acaaatcaa gcctactaa aactttgaa 
      ttcggaatc ggattcaag acaaagtaa tactgaggc 
      gagttcagt ccgaagtga gattaagtc aattgtgac 
      gtcaactga accggtcca gtctaactt aaccgaacc 
      ggaggaacc agaagtgga aaatgtgac tgaaagtgt 
      ggaattaga tgacccact tgatgcggc gacaccaca 
      ggctcagaa agaatcggt ttttttgcg cctttctaa 
      ccgaattaa agccctgga gcgcaacat gtccctctc 
      aggccaata cctcgaaga gttgagtaa acgatacaa 
      gaaacaagt cgaggagat acaaaaact cctcgacaa 
      aacaatact cagtttaaa cgaagaaag tcagaagaa 
      agacgaaag gaacaaaca gttggagga gcgacttaa 
      cgtgctaac ctcaaattt agaggccct tttggccga 
      acgcaggag ctggtcctc gaccatttc ctgcgtcgt 
      catttcctc tctagtcac tttatttat tgttacttt 
      ttgctgtaa aatccccga accggtggc ggcgagacg 
      gcccgagtt aaaacgacg aaaatcgat tgatgttgt 
      acaacacac cgcccccaa gaaaaaacg gcgtcccag 
      tgaccaagc tttaaagat cgtccggtc gtcaatgga 
      agctagtga taatttgag gaagaaaaa gaagaggaa 
      gaatatgct gaggcgatc gaattcgat tagtacgct 
      tggggggga ggagaaaaa ggcgtggcc tgaggcggg 
      gtctattga ctgggtgga ggggaagcg gtttgaaga 
      agtgccgtt tgtgttgga ggaaagtga gcactcgac 
      attggaacc aatcgcatt ggaagagaa aatcgcgcc 
      gcctagttt tgtgcaaac ggcggtgat ggcgagggc 
      ggtgcgagt caacggcga ggcgaagag ggaggagaa 
      tgggggcgt ggcagttga aggaacgcc caagaagga 
      gaagaaggg gttgaagag gatttttag gaaggtgtt 
      ttaagactg attgtaggc gtttttaag ttagttgag 
      ttatttata aaggtaata tatttttgt gtatttatt 
      taggaattc ctttttgag gcctgtgat aaaatttat 
      tttttaaac cgacatcgt agatgttgc atatttgta 
      aattttaat ttgcattgc ctgataata gagatctac 
      aaagtacat ggggtttta ctgctaact ttaatttca 
      ggataccca atttaaata aaaaaaaaa cacttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY