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Gene information
General information
TC013017 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15120443..15126316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013017
    iBeetle screen iB_05372

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013017 RA
      MDASSIITQ VSKDDEQLN SYPPEKGTL PRSENDLSE 
      EKIPSNNKK GNRGFFRSL FCCLGRKGT KKTNVEQCV 
      DGNQYIPGS SCTYLLPPV RHQDMHKKC MVIDLDETL 
      VHSSFKPIS NADFVVPVE IDGTVHQVY VLKRPHVDD 
      FLKRMGELY ECVLFTASL AKYADPVAD LLDQWGVFR 
      SRLFRESCV FYRGNYVKD LNKLGRELQ QIVIVDNSP 
      ASYIFHPDN AVPVASWFD DMSDSELLD LIPFFEKLS 
      KMDNVYTVL CNSNHPYNS SVSQNGPSN LASIQNNYS 
      PPNT
    CDS sequence
    • >TC013017 RA
      atggacgca tcgtccatt atcactcaa gtgtcaaag 
      gacgacgag cagttaaac agttatcct cccgaaaaa 
      ggaacgcta ccgaggagt gaaaatgac ctgtccgag 
      gagaaaata ccaagtaat aacaagaag ggcaacagg 
      ggcttcttc aggtccctg ttctgctgt ctggggcgg 
      aagggcacc aagaaaacc aacgttgag caatgtgtt 
      gacggtaac cagtacata ccggggagc agttgcacg 
      tacctttta ccgcccgtc cgacaccag gatatgcac 
      aagaaatgc atggtgatt gatttagat gagacactt 
      gttcacagc agttttaag ccaatcagc aacgccgac 
      ttcgtagtg cctgtcgag atcgacggc accgtccac 
      caggtgtac gtgttgaag cggccgcac gtggatgac 
      ttcctgaag cgtatggga gagctctac gaatgcgtc 
      cttttcact gcttcgtta gcgaaatat gcggatccg 
      gtggctgat ctgctcgat caatggggc gttttccgg 
      tctcgtctc tttagggaa agttgcgtc ttttatagg 
      ggaaattat gtgaaagat ttgaataag ttaggacgg 
      gagttgcag caaatcgta atcgtggat aattcacca 
      gcttcctac atattccat ccagataac gctgtgcca 
      gtggcgtcg tggttcgac gatatgagc gattcggag 
      cttttggac ctgattccc ttctttgag aagttgagt 
      aaaatggac aatgtgtat acagtgctg tgtaattcc 
      aatcatccg tataacagt agtgtgtca cagaatggc 
      ccctcgaac ctagccagt atacagaat aactactcg 
      ccgcccaat acgtag
    mRNA sequence
    • >TC013017 RA
      tgagtgtta aagcgaaaa cagcatctg ccattttgc 
      gaaggtgtt ttaaagatt atttattat cgtgcatgt 
      tgtgttcgg gtcaatcgg gcgtagcgc taagtttcc 
      gggggcccc aacaaatgt ttaaatgga cgcatcgtc 
      cattatcac tcaagtgtc aaaggacga cgagcagtt 
      aaacagtta tcctcccga aaaaggaac gctaccgag 
      gagtgaaaa tgacctgtc cgaggagaa aataccaag 
      taataacaa gaagggcaa caggggctt cttcaggtc 
      cctgttctg ctgtctggg gcggaaggg caccaagaa 
      aaccaacgt tgagcaatg tgttgacgg taaccagta 
      cataccggg gagcagttg cacgtacct tttaccgcc 
      cgtccgaca ccaggatat gcacaagaa atgcatggt 
      gattgattt agatgagac acttgttca cagcagttt 
      taagccaat cagcaacgc cgacttcgt agtgcctgt 
      cgagatcga cggcaccgt ccaccaggt gtacgtgtt 
      gaagcggcc gcacgtgga tgacttcct gaagcgtat 
      gggagagct ctacgaatg cgtcctttt cactgcttc 
      gttagcgaa atatgcgga tccggtggc tgatctgct 
      cgatcaatg gggcgtttt ccggtctcg tctctttag 
      ggaaagttg cgtctttta taggggaaa ttatgtgaa 
      agatttgaa taagttagg acgggagtt gcagcaaat 
      cgtaatcgt ggataattc accagcttc ctacatatt 
      ccatccaga taacgctgt gccagtggc gtcgtggtt 
      cgacgatat gagcgattc ggagctttt ggacctgat 
      tcccttctt tgagaagtt gagtaaaat ggacaatgt 
      gtatacagt gctgtgtaa ttccaatca tccgtataa 
      cagtagtgt gtcacagaa tggcccctc gaacctagc 
      cagtataca gaataacta ctcgccgcc caatacgta 
      gcgaaaagt tcctcgagt caaatttat tgccaaggt 
      cgttttcta tttcattca tggtttttg tttcggaga 
      gttcgattt tataccgac ctggtaatg tatttatta 
      cattacgaa tattatgga taagtgtaa atatgtagg 
      taaaaatag taacatatt ttttattaa gtgaaagaa 
      acaaaagta ttctatccc gcttaaaaa gttaccgaa 
      agctatatt taaagaaca ggtattttt acacggacg 
      tttcatgct ttttttttt gcttttatc cagggacct 
      acgtagcgt aagtccgaa cagacggag agagaatta 
      tagatagta gaccattta ttaatctgc attttttgt 
      ctttattta aaaaaaagt taaatactc aaagcattt 
      tatttttat cgatggcga ttattatgt tagtatagt 
      cgagtgtgt ttcgtactt gtactaaaa actcgtaaa 
      acaaaataa aaaaagcga tcgatgtga ctaaacaat 
      caatcatat ctcgtttcg aggtgatgt tatttgtgg 
      gaacaagtc gttccaatt gttaacagt ggccttaaa 
      acaaatatc tgaactttt ttgaacatt tatgaaaat 
      tttactgta gatccgtca cttctacag gaaaaccgc 
      tcggtgacg ttaaaatta tgccttcaa gcatcactg 
      taactgtca agcaatggc agattattg taagatcgg 
      tttgtgtat tgagtacaa aaattttat tttaaatct 
      tataaattc tttgataag ttttaaagt aaagttagt 
      cggtttaag ttatattat ttttcttga tttaaaatc 
      gctgtacag acatatatg ttatttagt aatatattc 
      ttactatta agattttat taatatatt ataaatgta 
      tatttatcc attgtattg tgtgaaatt ctatttttt 
      tttaaatga gcaatattt tacctgcct cacatccaa 
      cataatgga ccctttcgt taggttaag gtttttata 
      tccaaatta accgggttt ggttattag aaacagcaa 
      tagtacaat gtggggtga gaatggagt aatttagct 
      aattaacat tatttttta cacgattaa actaatttg 
      ttgttgtag agaattata attattatt gtttatgtt 
      gatatatag tatacctgt acattttaa acacttgta 
      caaaaatga gtgttttca tataattgt catgtgaat 
      acatatgcc ttgatttgt aaataaaag cctgtcatt 
      tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY