Status
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Gene information
General information
TC013017 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15120443..15126316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013017
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05372

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013017 RA
      MDASSIITQ VSKDDEQLN SYPPEKGTL PRSENDLSE 
EKIPSNNKK GNRGFFRSL FCCLGRKGT KKTNVEQCV 
DGNQYIPGS SCTYLLPPV RHQDMHKKC MVIDLDETL 
VHSSFKPIS NADFVVPVE IDGTVHQVY VLKRPHVDD 
FLKRMGELY ECVLFTASL AKYADPVAD LLDQWGVFR 
SRLFRESCV FYRGNYVKD LNKLGRELQ QIVIVDNSP 
ASYIFHPDN AVPVASWFD DMSDSELLD LIPFFEKLS 
KMDNVYTVL CNSNHPYNS SVSQNGPSN LASIQNNYS 
PPNT
    CDS sequence
    • >TC013017 RA
      atggacgca tcgtccatt atcactcaa gtgtcaaag 
gacgacgag cagttaaac agttatcct cccgaaaaa 
ggaacgcta ccgaggagt gaaaatgac ctgtccgag 
gagaaaata ccaagtaat aacaagaag ggcaacagg 
ggcttcttc aggtccctg ttctgctgt ctggggcgg 
aagggcacc aagaaaacc aacgttgag caatgtgtt 
gacggtaac cagtacata ccggggagc agttgcacg 
tacctttta ccgcccgtc cgacaccag gatatgcac 
aagaaatgc atggtgatt gatttagat gagacactt 
gttcacagc agttttaag ccaatcagc aacgccgac 
ttcgtagtg cctgtcgag atcgacggc accgtccac 
caggtgtac gtgttgaag cggccgcac gtggatgac 
ttcctgaag cgtatggga gagctctac gaatgcgtc 
cttttcact gcttcgtta gcgaaatat gcggatccg 
gtggctgat ctgctcgat caatggggc gttttccgg 
tctcgtctc tttagggaa agttgcgtc ttttatagg 
ggaaattat gtgaaagat ttgaataag ttaggacgg 
gagttgcag caaatcgta atcgtggat aattcacca 
gcttcctac atattccat ccagataac gctgtgcca 
gtggcgtcg tggttcgac gatatgagc gattcggag 
cttttggac ctgattccc ttctttgag aagttgagt 
aaaatggac aatgtgtat acagtgctg tgtaattcc 
aatcatccg tataacagt agtgtgtca cagaatggc 
ccctcgaac ctagccagt atacagaat aactactcg 
ccgcccaat acgtag
    mRNA sequence
    • >TC013017 RA
      tgagtgtta aagcgaaaa cagcatctg ccattttgc 
gaaggtgtt ttaaagatt atttattat cgtgcatgt 
tgtgttcgg gtcaatcgg gcgtagcgc taagtttcc 
gggggcccc aacaaatgt ttaaatgga cgcatcgtc 
cattatcac tcaagtgtc aaaggacga cgagcagtt 
aaacagtta tcctcccga aaaaggaac gctaccgag 
gagtgaaaa tgacctgtc cgaggagaa aataccaag 
taataacaa gaagggcaa caggggctt cttcaggtc 
cctgttctg ctgtctggg gcggaaggg caccaagaa 
aaccaacgt tgagcaatg tgttgacgg taaccagta 
cataccggg gagcagttg cacgtacct tttaccgcc 
cgtccgaca ccaggatat gcacaagaa atgcatggt 
gattgattt agatgagac acttgttca cagcagttt 
taagccaat cagcaacgc cgacttcgt agtgcctgt 
cgagatcga cggcaccgt ccaccaggt gtacgtgtt 
gaagcggcc gcacgtgga tgacttcct gaagcgtat 
gggagagct ctacgaatg cgtcctttt cactgcttc 
gttagcgaa atatgcgga tccggtggc tgatctgct 
cgatcaatg gggcgtttt ccggtctcg tctctttag 
ggaaagttg cgtctttta taggggaaa ttatgtgaa 
agatttgaa taagttagg acgggagtt gcagcaaat 
cgtaatcgt ggataattc accagcttc ctacatatt 
ccatccaga taacgctgt gccagtggc gtcgtggtt 
cgacgatat gagcgattc ggagctttt ggacctgat 
tcccttctt tgagaagtt gagtaaaat ggacaatgt 
gtatacagt gctgtgtaa ttccaatca tccgtataa 
cagtagtgt gtcacagaa tggcccctc gaacctagc 
cagtataca gaataacta ctcgccgcc caatacgta 
gcgaaaagt tcctcgagt caaatttat tgccaaggt 
cgttttcta tttcattca tggtttttg tttcggaga 
gttcgattt tataccgac ctggtaatg tatttatta 
cattacgaa tattatgga taagtgtaa atatgtagg 
taaaaatag taacatatt ttttattaa gtgaaagaa 
acaaaagta ttctatccc gcttaaaaa gttaccgaa 
agctatatt taaagaaca ggtattttt acacggacg 
tttcatgct ttttttttt gcttttatc cagggacct 
acgtagcgt aagtccgaa cagacggag agagaatta 
tagatagta gaccattta ttaatctgc attttttgt 
ctttattta aaaaaaagt taaatactc aaagcattt 
tatttttat cgatggcga ttattatgt tagtatagt 
cgagtgtgt ttcgtactt gtactaaaa actcgtaaa 
acaaaataa aaaaagcga tcgatgtga ctaaacaat 
caatcatat ctcgtttcg aggtgatgt tatttgtgg 
gaacaagtc gttccaatt gttaacagt ggccttaaa 
acaaatatc tgaactttt ttgaacatt tatgaaaat 
tttactgta gatccgtca cttctacag gaaaaccgc 
tcggtgacg ttaaaatta tgccttcaa gcatcactg 
taactgtca agcaatggc agattattg taagatcgg 
tttgtgtat tgagtacaa aaattttat tttaaatct 
tataaattc tttgataag ttttaaagt aaagttagt 
cggtttaag ttatattat ttttcttga tttaaaatc 
gctgtacag acatatatg ttatttagt aatatattc 
ttactatta agattttat taatatatt ataaatgta 
tatttatcc attgtattg tgtgaaatt ctatttttt 
tttaaatga gcaatattt tacctgcct cacatccaa 
cataatgga ccctttcgt taggttaag gtttttata 
tccaaatta accgggttt ggttattag aaacagcaa 
tagtacaat gtggggtga gaatggagt aatttagct 
aattaacat tatttttta cacgattaa actaatttg 
ttgttgtag agaattata attattatt gtttatgtt 
gatatatag tatacctgt acattttaa acacttgta 
caaaaatga gtgttttca tataattgt catgtgaat 
acatatgcc ttgatttgt aaataaaag cctgtcatt 
tat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY