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Gene information
General information
TC033271
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013014
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15245645..15248226
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033271
    iBeetle screen iB_05371

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    wings slightlynot closed - Number of animals showing the phenotype: 5

    wings partiallyblistered - Number of animals showing the phenotype: 2

    Adult morphology
    wings partially swollen - Number of animals showing the phenotype: 1

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033271 RA
      MKIRHRGPD ASRVETDKR VTNCCVAFH RLAIVDCIY 
      GMQPMRLHQ FPLTLLICN GELYNCKEL EKQQGFKYE 
      TYCDVECIT HLYHKLGIE GCVKHLDGV YAFCLIDME 
      KQKVFLGRD PYGVRPLFK LTTSDGTLG ICSEAKGLI 
      GLAREENGE KKTIKPFTP GTFEEYDLM KTGGVKLVR 
      KQTFHHPGD KPTFQPFVL YEELTHDPL ENIRKLLTT 
      AVKKRLMAN RRIGCFLSG GLDSSLIAS LLVRAAKEV 
      DLPYKIQTF CIGMGDSPD VIAAKEVAD FIGTEHHVI 
      TFSEEDVAR VLDDVIYTL ETPDITTIR ASIPMYLIS 
      RYVLENTDT TVLFSGEGA DELCQGYIY FRDAPSAQA 
      GHDDSIRLL KEIYLFDGL RGDRTTAAS SLELRLPFL 
      DLQFTHYYL SLDPKLRQP KDGVEKFLI RSAFDKTGV 
      LPDRILWRH KEAFSDGVA SKKKSLFQF LQEIVDKRM 
      PEPFDLEAA KKKYPHCTP NSKESLYYR EVFEKSFPN 
      AAEKFIPFY WMPRWVEGV TDPSARFIK HYAAPQK
    CDS sequence
    • >TC033271 RA
      atgaaaata agacatcgg gggcccgac gcttcacgt 
      gtcgagacg gacaagcgg gtcactaac tgttgtgtg 
      gcattccac cgcttggca atagtcgat tgcatttac 
      ggaatgcaa ccgatgcgc cttcaccaa tttccccta 
      actctccta atatgcaat ggcgagtta tacaactgc 
      aaagaattg gagaaacaa caaggcttc aaatatgag 
      acttattgc gacgttgaa tgcatcacc catctgtac 
      cacaagctt ggtattgaa gggtgcgtc aaacacttg 
      gatggcgtt tacgccttc tgtctcata gacatggaa 
      aagcagaaa gttttcctg gggagagac ccgtacggc 
      gtacgccct ttattcaag ctcacgact tcggacgga 
      acgttgggc atttgctcc gaagccaaa ggtttgatt 
      ggtttagcg agagaagaa aacggggaa aagaaaaca 
      atcaagcct ttcacaccc ggaactttc gaagaatac 
      gacttgatg aaaaccggg ggagttaag ctagtccgg 
      aaacaaacc ttccaccac cctggcgac aaaccaacc 
      ttccaaccg ttcgtttta tacgaagaa ctaacgcac 
      gacccgttg gaaaacata aggaaattg ctaacaacg 
      gccgttaag aagcggtta atggcaaac cggcggatt 
      ggctgtttc ttatccgga gggctagac tcatccctt 
      attgcctct ctgctggtc agagcggcc aaagaagta 
      gaccttcca tacaaaatt caaacgttt tgcattggt 
      atgggcgac agccctgat gtgatcgcg gcaaaagaa 
      gtggccgac tttatcggc acggagcac cacgtgatc 
      actttcagc gaagaggac gtagctcgg gtccttgat 
      gacgttatc tacactctc gaaacgccc gacattacc 
      acaatcagg gcctcaatc cccatgtat ttgatttcg 
      cgctatgta ttggaaaac accgatact acagttctc 
      ttcagtgga gagggcgct gatgagctt tgtcaagga 
      tacatctat ttcagagac gctccaagc gcgcaagca 
      ggccatgac gacagcatc cgtctcctt aaagaaatc 
      taccttttc gacggctta aggggcgat agaaccaca 
      gctgcgagc agtcttgag ctgcgattg cctttcctt 
      gacttgcag tttacgcat tattatctc agtcttgac 
      ccgaaattg cggcagccg aaagatggc gttgagaag 
      tttttgata agatcggcg tttgataag acgggggtg 
      ttgccagat cggattttg tggaggcat aaggaggcg 
      ttcagtgac ggggtggcg tccaagaag aagtcgctg 
      tttcaattc ctccaagag attgttgat aagaggatg 
      cctgaaccg tttgatttg gaagctgcg aaaaaaaag 
      tacccacat tgtactccc aacagtaaa gagtcgctt 
      tattatcgc gaagttttc gaaaagagt tttccgaat 
      gcggcggag aagtttatt cccttctat tggatgccg 
      agatgggtt gaaggtgtc acagatcct tcagcacgg 
      tttatcaaa cattatgcg gctccgcaa aagtaa
    mRNA sequence
    • >TC033271 RA
      acgaaaagt tattacaaa ttcattttt tctcaattt 
      ttaattaaa atattattt tttcaggtg aagtcgctg 
      acttgtacg agttcaagg cctcaggtc gttattttg 
      gcaagtaat aaaaaataa aattcggat tttcgttta 
      cgaaagcac ccgagcttg gccgaagtg tgattgtgg 
      cgacacgtg gttgtggtt ccagctaac gtcaagtgc 
      ggctttttg tataaaaaa gcagaattt tgcattaat 
      tcaacagtt taacacgtg gaacagttt caacgagtc 
      gttttcaac acaatgtgt ggaatttgg gctttattt 
      caccctcaa aatggcaat gtcctgctc aaggattgt 
      acagagaac gttatgaaa ataagacat cgggggccc 
      gacgcttca cgtgtcgag acggacaag cgggtcact 
      aactgttgt gtggcattc caccgcttg gcaatagtc 
      gattgcatt tacggaatg caaccgatg cgccttcac 
      caatttccc ctaactctc ctaatatgc aatggcgag 
      ttatacaac tgcaaagaa ttggagaaa caacaaggc 
      ttcaaatat gagacttat tgcgacgtt gaatgcatc 
      acccatctg taccacaag cttggtatt gaagggtgc 
      gtcaaacac ttggatggc gtttacgcc ttctgtctc 
      atagacatg gaaaagcag aaagttttc ctggggaga 
      gacccgtac ggcgtacgc cctttattc aagctcacg 
      acttcggac ggaacgttg ggcatttgc tccgaagcc 
      aaaggtttg attggttta gcgagagaa gaaaacggg 
      gaaaagaaa acaatcaag cctttcaca cccggaact 
      ttcgaagaa tacgacttg atgaaaacc gggggagtt 
      aagctagtc cggaaacaa accttccac caccctggc 
      gacaaacca accttccaa ccgttcgtt ttatacgaa 
      gaactaacg cacgacccg ttggaaaac ataaggaaa 
      ttgctaaca acggccgtt aagaagcgg ttaatggca 
      aaccggcgg attggctgt ttcttatcc ggagggcta 
      gactcatcc cttattgcc tctctgctg gtcagagcg 
      gccaaagaa gtagacctt ccatacaaa attcaaacg 
      ttttgcatt ggtatgggc gacagccct gatgtgatc 
      gcggcaaaa gaagtggcc gactttatc ggcacggag 
      caccacgtg atcactttc agcgaagag gacgtagct 
      cgggtcctt gatgacgtt atctacact ctcgaaacg 
      cccgacatt accacaatc agggcctca atccccatg 
      tatttgatt tcgcgctat gtattggaa aacaccgat 
      actacagtt ctcttcagt ggagagggc gctgatgag 
      ctttgtcaa ggatacatc tatttcaga gacgctcca 
      agcgcgcaa gcaggccat gacgacagc atccgtctc 
      cttaaagaa atctacctt ttcgacggc ttaaggggc 
      gatagaacc acagctgcg agcagtctt gagctgcga 
      ttgcctttc cttgacttg cagtttacg cattattat 
      ctcagtctt gacccgaaa ttgcggcag ccgaaagat 
      ggcgttgag aagtttttg ataagatcg gcgtttgat 
      aagacgggg gtgttgcca gatcggatt ttgtggagg 
      cataaggag gcgttcagt gacggggtg gcgtccaag 
      aagaagtcg ctgtttcaa ttcctccaa gagattgtt 
      gataagagg atgcctgaa ccgtttgat ttggaagct 
      gcgaaaaaa aagtaccca cattgtact cccaacagt 
      aaagagtcg ctttattat cgcgaagtt ttcgaaaag 
      agttttccg aatgcggcg gagaagttt attcccttc 
      tattggatg ccgagatgg gttgaaggt gtcacagat 
      ccttcagca cggtttatc aaacattat gcggctccg 
      caaaagtaa agtacaaat ttattgtaa attataaat 
      aaagttttc tcgaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY