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Gene information
General information
TC013003 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15340557..15342556
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013003
    iBeetle screen iB_05370

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013003 RA
      MTPEPRTRK STRLHLKQT SSESVNTRR RTRKPIIIS 
      DSEEENVPE TEKSPFKKP PNTPYKTKS RTPPEDRTV 
      TPPKQRKMS ENNENLQTP PSTPTILLE RLSLLSPNE 
      KSNRKKLFQ EDDLYQNAR RALHSSCPT NLPGREKEL 
      GDLKQFILQ HLDEGTSGT LYISGPPGT GKTASLNLV 
      LEDPQISSG IEHVYVNCT SIKSSGSIF SRIAKDLGI 
      KASGKSEKD YVGAIEKFL QKGHRTILL VLDEIDQLE 
      SKKQSVLYT IFEWPANPN SRLILIGIA NALDLTDRI 
      LPRLQARCE LKPQLMHFA PYTKQQIVE IFTNRLKNA 
      NVLDIFSPI ALQMLAGKV AAISGDVRR ALDIGRRVI 
      EMSDKKKRG ECVLKSVEN LATELEEKK LESVDMRQV 
      VQVLNRVYG TTQSLDEEV EDSFPLQQK IIVCSLLLI 
      LKKAKNKDV TIGRLHDVY RRVCAKRNI QVVDQGEFV 
      GLCSLIETR GMVKIIKKK DPRMNKVSL VWDEGEVSA 
      TLRDKQLMS MILQDDSCL GKM
    CDS sequence
    • >TC013003 RA
      atgactccg gaaccacgt accagaaaa tcaacaaga 
      cttcacctg aagcaaact tcttcagaa tcagtgaac 
      actagacga agaacccga aaaccaatc ataatatca 
      gatagtgaa gaagaaaat gtgcctgag accgagaaa 
      tcgccgttc aaaaagccc ccaaacacc ccctacaaa 
      accaaatcc agaactccc cccgaagat cggactgtg 
      acgcccccc aaacaacgc aaaatgagt gaaaataat 
      gagaatcta caaactccc ccatccaca cccacaatt 
      ttgctcgaa agactgtcg ctgctgagt cccaacgaa 
      aaatcaaac cggaaaaag ttatttcaa gaagacgat 
      ttgtatcag aatgccagg agagcttta cacagttca 
      tgtcccacg aatttgcca ggtcgtgaa aaggagttg 
      ggggatttg aaacagttt attttgcaa catttggac 
      gaaggaact tccggtact ttgtatata agtgggccc 
      ccaggaacg ggcaagacc gcttcgttg aacttggtt 
      ttggaggat cctcaaatc tcatcaggg attgaacac 
      gtttatgtt aattgtacg tcgatcaag tcctcaggg 
      tcgattttt tcgaggatt gccaaggat ttagggatt 
      aaggccagt gggaagagt gagaaggat tacgtgggg 
      gcgattgag aagttcctt caaaaagga cacaggacg 
      attctgctc gtccttgac gagatcgac caattagag 
      agcaaaaaa cagtcggtc ctttacaca attttcgaa 
      tggccagca aatcccaac tcgcgcttg attctaata 
      gggattgca aatgcgcta gacttgacc gaccgaatc 
      cttcctcgt ctgcaggcc cgttgcgag ttaaaaccc 
      caattaatg catttcgct ccttacacc aaacaacaa 
      attgtcgaa atattcaca aatcgtttg aaaaatgcc 
      aatgttttg gacatattc tcgccaatc gccttacaa 
      atgttagcg ggtaaagta gccgcaatt tcgggggac 
      gttcggcgc gctttagac attgggaga cgagtaatt 
      gaaatgtct gataagaaa aaacgcggt gaatgtgtg 
      ctcaaatcc gttgaaaat ttagcaacg gaattggag 
      gagaaaaag cttgaaagt gttgatatg agacaagtc 
      gttcaagtt ttgaacaga gtctacgga acgacgcaa 
      agtctggat gaggaagtt gaagattcg ttcccgttg 
      caacagaaa attattgtt tgttcgttg ttgttgatt 
      ttgaaaaag gcgaaaaat aaggatgtg acgattgga 
      cgactgcat gatgtgtat aggagggtt tgtgccaaa 
      aggaatatt caagttgtt gatcaaggc gagtttgtc 
      ggactttgt tccctgatt gagaccaga gggatggtg 
      aaaattatt aaaaagaag gatcctagg atgaacaaa 
      gttagcttg gtttgggac gaaggcgaa gttagtgcc 
      actttgagg gacaaacag ctgatgtcc atgatttta 
      caggacgat agttgttta ggcaaaatg taa
    mRNA sequence
    • >TC013003 RA
      aaaagcgcg aaaagtgac aagtgtcag tacatggtg 
      gctgtttta accctcatt aaagtgcca ttattatct 
      cattaacta cataaaagt ggtgattgc aagcttccc 
      ttgctcata attttagaa ctttgattt gtgtgcctc 
      atcaaaatg actccggaa ccacgtacc agaaaatca 
      acaagactt cacctgaag caaacttct tcagaatca 
      gtgaacact agacgaaga acccgaaaa ccaatcata 
      atatcagat agtgaagaa gaaaatgtg cctgagacc 
      gagaaatcg ccgttcaaa aagccccca aacaccccc 
      tacaaaacc aaatccaga actcccccc gaagatcgg 
      actgtgacg ccccccaaa caacgcaaa atgagtgaa 
      aataatgag aatctacaa actccccca tccacaccc 
      acaattttg ctcgaaaga ctgtcgctg ctgagtccc 
      aacgaaaaa tcaaaccgg aaaaagtta tttcaagaa 
      gacgatttg tatcagaat gccaggaga gctttacac 
      agttcatgt cccacgaat ttgccaggt cgtgaaaag 
      gagttgggg gatttgaaa cagtttatt ttgcaacat 
      ttggacgaa ggaacttcc ggtactttg tatataagt 
      gggccccca ggaacgggc aagaccgct tcgttgaac 
      ttggttttg gaggatcct caaatctca tcagggatt 
      gaacacgtt tatgttaat tgtacgtcg atcaagtcc 
      tcagggtcg attttttcg aggattgcc aaggattta 
      gggattaag gccagtggg aagagtgag aaggattac 
      gtgggggcg attgagaag ttccttcaa aaaggacac 
      aggacgatt ctgctcgtc cttgacgag atcgaccaa 
      ttagagagc aaaaaacag tcggtcctt tacacaatt 
      ttcgaatgg ccagcaaat cccaactcg cgcttgatt 
      ctaataggg attgcaaat gcgctagac ttgaccgac 
      cgaatcctt cctcgtctg caggcccgt tgcgagtta 
      aaaccccaa ttaatgcat ttcgctcct tacaccaaa 
      caacaaatt gtcgaaata ttcacaaat cgtttgaaa 
      aatgccaat gttttggac atattctcg ccaatcgcc 
      ttacaaatg ttagcgggt aaagtagcc gcaatttcg 
      ggggacgtt cggcgcgct ttagacatt gggagacga 
      gtaattgaa atgtctgat aagaaaaaa cgcggtgaa 
      tgtgtgctc aaatccgtt gaaaattta gcaacggaa 
      ttggaggag aaaaagctt gaaagtgtt gatatgaga 
      caagtcgtt caagttttg aacagagtc tacggaacg 
      acgcaaagt ctggatgag gaagttgaa gattcgttc 
      ccgttgcaa cagaaaatt attgtttgt tcgttgttg 
      ttgattttg aaaaaggcg aaaaataag gatgtgacg 
      attggacga ctgcatgat gtgtatagg agggtttgt 
      gccaaaagg aatattcaa gttgttgat caaggcgag 
      tttgtcgga ctttgttcc ctgattgag accagaggg 
      atggtgaaa attattaaa aagaaggat cctaggatg 
      aacaaagtt agcttggtt tgggacgaa ggcgaagtt 
      agtgccact ttgagggac aaacagctg atgtccatg 
      attttacag gacgatagt tgtttaggc aaaatgtaa 
      ttttttaaa gactgttcc tgtcgaata gaattttat 
      ttattgatt gtaaacacg atgtaatgt ttcttaaaa 
      taatttttt ttttaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY