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Gene information
General information
TC034711 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15348309..15350126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034711
    iBeetle screen iB_05369

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034711 RA
      MEICGAPLN RTGLQRVAL KDMEPDMDN ILIVAIIIG 
      KQRPRKFLD KTKETEQYK AVWTFTVRD SPRDYINVT 
      YWGSSDDVC TASDKYYTG DVVEIRNPR INVRKFNET 
      GEQFCPIVS SPYSLTLNE HSTIFPFNG DTSFLTKLL 
      NFPTKPLTA FVPLRDIRN EGANLKERF VDILGAIRS 
      VGNVRTITT KRGQQMQLR EIVIFDHTS SGLKISIWD 
      PDIITRASM WKPRETILF ITDAKVEWS MFLKTYCAA 
      VTGRSIITE NPLGQEAAT LANYAKSAP LTTADIMDQ 
      FITELPDPE SIQNVMCVQ QVFAKIRNL QENAVSDSQ 
      LTALMFVFI TQLDLDGLS PVTRTKCAY CKSFLANEK 
      SICENSECS TFLGKTPNN VEVSFNINV HLSDHTGTL 
      KNCRLTGTS AEQVLNCSV QRFVKMTDS QKCELKWKF 
      LLERCAVRI LAVMSSRLN PRIVIISCS QANALESAQ 
      RIPIC
    CDS sequence
    • >TC034711 RA
      atggaaatt tgtggcgct cctttgaac agaacaggg 
      ctccagaga gtggctttg aaggacatg gagcccgac 
      atggacaac attttaata gtggcgata attatcggc 
      aagcagaga ccgcgaaaa tttctcgat aaaaccaaa 
      gaaacggag caatacaaa gcggtttgg acgtttact 
      gtacgagat tcgccacga gattacatc aatgtgacc 
      tactggggc tccagtgac gatgtttgt actgctagt 
      gataagtac tacactggg gatgttgtt gaaatacga 
      aacccgcga atcaatgtc cgaaaattt aacgagacc 
      ggtgagcag ttttgtccc attgtgagt tccccctac 
      tccctcact ttgaatgaa cattcgact atttttccc 
      ttcaatgga gatacttca ttcctgacg aagttgtta 
      aatttcccc acgaaacct ctgactgcg tttgttcct 
      ctccgtgat attcgcaat gaaggggct aacttgaaa 
      gagcgcttt gttgatatt ctgggagct ataaggagc 
      gtgggaaac gtgcgcacg atcactacg aaaaggggg 
      caacaaatg caactgcga gaaattgtc attttcgat 
      cacaccagt tctgggtta aaaatatca atttgggac 
      ccagatatt ataacgaga gcgagtatg tggaagccg 
      cgcgaaacc attctcttt ataactgac gcaaaagta 
      gaatggtca atgtttttg aaaacttac tgtgctgca 
      gtcactggt cgttcaatt attacggaa aatccacta 
      ggacaagaa gctgcaact ttggccaat tacgccaaa 
      agcgctcct ttgacaacg gcggatatt atggatcag 
      tttatcaca gaattaccg gatcctgaa tcgatccag 
      aacgtgatg tgtgtgcaa caagttttt gccaaaatt 
      cgtaatttg caagaaaat gcagtatca gactcgcag 
      ctaacagct ctcatgttt gttttcata actcagtta 
      gatttagac ggtctttca cccgtaaca cgaaccaag 
      tgtgcttat tgtaaaagt tttctcgca aatgaaaag 
      tccatttgt gaaaacagc gaatgctcc acattttta 
      ggtaaaacc cctaacaat gttgaagtc tcctttaat 
      attaacgtt catttatct gatcatact gggactcta 
      aaaaactgt cgtttaacg ggtacctcc gctgagcaa 
      gtattgaac tgctcagtt cagcgtttt gttaaaatg 
      accgacagt caaaagtgc gaattaaaa tggaaattt 
      ttattggaa cgttgtgct gttcgtatt ttagcggta 
      atgtccagt agattaaat cctcgcatt gtaatcata 
      agttgctcg caagcaaat gcgctagaa tcagcacaa 
      agaataccg atttgttaa
    mRNA sequence
    • >TC034711 RA
      aataacaat tgaaaaaga cgcttccac cattttgcc 
      aaatttggt tgttggttt gttgattgt taagtgtcg 
      taataaaat ggaaatttg tggcgctcc tttgaacag 
      aacagggct ccagagagt ggctttgaa ggacatgga 
      gcccgacat ggacaacat tttaatagt ggcgataat 
      tatcggcaa gcagagacc gcgaaaatt tctcgataa 
      aaccaaaga aacggagca atacaaagc ggtttggac 
      gtttactgt acgagattc gccacgaga ttacatcaa 
      tgtgaccta ctggggctc cagtgacga tgtttgtac 
      tgctagtga taagtacta cactgggga tgttgttga 
      aatacgaaa cccgcgaat caatgtccg aaaatttaa 
      cgagaccgg tgagcagtt ttgtcccat tgtgagttc 
      cccctactc cctcacttt gaatgaaca ttcgactat 
      ttttccctt caatggaga tacttcatt cctgacgaa 
      gttgttaaa tttccccac gaaacctct gactgcgtt 
      tgttcctct ccgtgatat tcgcaatga aggggctaa 
      cttgaaaga gcgctttgt tgatattct gggagctat 
      aaggagcgt gggaaacgt gcgcacgat cactacgaa 
      aagggggca acaaatgca actgcgaga aattgtcat 
      tttcgatca caccagttc tgggttaaa aatatcaat 
      ttgggaccc agatattat aacgagagc gagtatgtg 
      gaagccgcg cgaaaccat tctctttat aactgacgc 
      aaaagtaga atggtcaat gtttttgaa aacttactg 
      tgctgcagt cactggtcg ttcaattat tacggaaaa 
      tccactagg acaagaagc tgcaacttt ggccaatta 
      cgccaaaag cgctccttt gacaacggc ggatattat 
      ggatcagtt tatcacaga attaccgga tcctgaatc 
      gatccagaa cgtgatgtg tgtgcaaca agtttttgc 
      caaaattcg taatttgca agaaaatgc agtatcaga 
      ctcgcagct aacagctct catgtttgt tttcataac 
      tcagttaga tttagacgg tctttcacc cgtaacacg 
      aaccaagtg tgcttattg taaaagttt tctcgcaaa 
      tgaaaagtc catttgtga aaacagcga atgctccac 
      atttttagg taaaacccc taacaatgt tgaagtctc 
      ctttaatat taacgttca tttatctga tcatactgg 
      gactctaaa aaactgtcg tttaacggg tacctccgc 
      tgagcaagt attgaactg ctcagttca gcgttttgt 
      taaaatgac cgacagtca aaagtgcga attaaaatg 
      gaaattttt attggaacg ttgtgctgt tcgtatttt 
      agcggtaat gtccagtag attaaatcc tcgcattgt 
      aatcataag ttgctcgca agcaaatgc gctagaatc 
      agcacaaag aataccgat ttgttaatt taagtttaa 
      taaaaaagc gaataactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY