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Gene information
General information
TC012996 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15419138..15421176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012996
    iBeetle screen iB_05367

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012996 RA
      MEEEISQKI CRACLTESG EFQSIFVPD EKTGTSIHI 
      SEMMMAFAT VQVTLGDGL PEQICSTCA RTTVDMYMF 
      KLKCEESEK ILRSRLAKS PFQEQFTIE EKHYVETKG 
      DVDVYSIVI KPELCSEDS EINHELSSD FEHLNAPDK 
      SDSDLDAFE LYADEGGQE HRCPHCPKV FDKESAANR 
      HIKTHSAVK AYKCDTCDK RFSRNDLLL RHKIAHAMK 
      MDDQKFELE NFSDDDEDP DDDTIIIKT ETALYPCAE 
      CNLIFIKKE ELDAHIQKH EKGGGEGIV CNVCMKTFS 
      KQAHLNRHK KIHSQNKPH VCPTCNKGF VRAEQLNNH 
      MNIHSGIKP HVCAICLKG FNQISNLKD HMRTHNGEK 
      PFLCSTCGK GFNQLGNLR QHTIRHSGI KAHLCSTCG 
      NGFASKGEL SAHLRKHTG ARPFVCPIC NHGFTTSSS 
      LTKHKRIHS GEKPYECDV CKMKFSRSG ILARHKRTH 
      TGEKPYVCK FCTKAFSQS NDLTSHLRI HTGEKPYIC 
      DVCGQAFRQ SSALKTHKK THPGREVID TKIGVLGVM 
      HTPFLT
    CDS sequence
    • >TC012996 RA
      atggaggag gaaatctcc caaaaaatt tgtcgtgcc 
      tgtttgacc gagtctggc gaattccag tcgatcttc 
      gtccccgat gagaaaacc ggcacttcc atacacatt 
      tctgaaatg atgatggca ttcgctacc gtccaggta 
      actcttggt gacggtctc ccggaacaa atctgctcc 
      acgtgcgcc cgcaccact gtagatatg tacatgttc 
      aagttaaag tgcgaagaa tcggagaaa atcctgcga 
      agtcggctg gccaaaagc cccttccag gagcagttc 
      accatcgag gagaagcac tacgtggag accaagggg 
      gacgtggac gtttacagc atagtgatc aagcctgag 
      ctgtgctcc gaagactcg gaaatcaac cacgagctc 
      agctcagac tttgagcat ttgaacgca ccggataaa 
      agtgacagt gatttggac gcgtttgag ttgtacgcg 
      gatgaaggg ggccaggag caccggtgt ccccattgc 
      cccaaagtg ttcgataag gaaagtgcg gccaatcgc 
      cacatcaaa acacattcg gctgttaaa gcttacaag 
      tgtgatacg tgtgataag agattttcg cggaatgat 
      cttctatta cggcacaaa attgcacat gcgatgaaa 
      atggacgac caaaaattc gaattggaa aatttcagc 
      gatgatgat gaagatccg gatgatgac acgattatt 
      attaaaact gaaacggct ttgtatcca tgtgctgaa 
      tgcaatctc atttttatt aagaaagag gaacttgat 
      gcacacatt caaaagcat gaaaaaggg ggtggcgaa 
      ggaattgtt tgtaatgtt tgtatgaaa acgttcagc 
      aaacaagcg catttgaat cgacataag aaaattcat 
      tcgcagaat aagccacat gtttgtccg acctgtaat 
      aagggattt gtgagggcg gaacagctt aataaccac 
      atgaatata cattctgga atcaaaccg catgtctgt 
      gcgatttgt ctcaaaggt tttaaccaa atatcaaat 
      ttgaaagac cacatgcga acccacaac ggtgaaaag 
      ccctttctt tgttccacc tgtggcaag ggcttcaac 
      cagttggga aaccttcgt caacacacc atccgccac 
      agcggcatt aaagcccac ctttgcagt acgtgtggt 
      aacggtttc gccagcaaa ggtgaactt tcagcccat 
      ctccgaaaa cacaccggt gcgcgccct ttcgtgtgt 
      ccaatctgt aaccacggc tttacaact tcaagttcg 
      ttaacaaaa cacaaacga atccattcc ggggaaaaa 
      ccgtacgag tgtgacgta tgcaaaatg aagttttcg 
      cggtcgggg attctagcc agacataaa aggacgcac 
      acgggagag aaaccttac gtttgcaag ttttgtaca 
      aaagcattt tcacagtcg aatgatctg acgtcacat 
      ttgcggatt cacacgggg gaaaaaccg tacatttgc 
      gatgtatgt ggacaggca tttaggcag agttctgcg 
      cttaaaact cataagaaa acgcatcct ggacgagag 
      gttattgac acgaaaatt ggggtcttg ggggtcatg 
      cataccccc tttttaacg taa
    mRNA sequence
    • >TC012996 RA
      cgttcgatt gattgtcaa aagtttggt tgcaatttt 
      aacctaata aaaataatg gaggaggaa atctcccaa 
      aaaatttgt cgtgcctgt ttgaccgag tctggcgaa 
      ttccagtcg atcttcgtc cccgatgag aaaaccggc 
      acttccata cacatttct gaaatgatg atggcattc 
      gctaccgtc caggtaact cttggtgac ggtctcccg 
      gaacaaatc tgctccacg tgcgcccgc accactgta 
      gatatgtac atgttcaag ttaaagtgc gaagaatcg 
      gagaaaatc ctgcgaagt cggctggcc aaaagcccc 
      ttccaggag cagttcacc atcgaggag aagcactac 
      gtggagacc aagggggac gtggacgtt tacagcata 
      gtgatcaag cctgagctg tgctccgaa gactcggaa 
      atcaaccac gagctcagc tcagacttt gagcatttg 
      aacgcaccg gataaaagt gacagtgat ttggacgcg 
      tttgagttg tacgcggat gaagggggc caggagcac 
      cggtgtccc cattgcccc aaagtgttc gataaggaa 
      agtgcggcc aatcgccac atcaaaaca cattcggct 
      gttaaagct tacaagtgt gatacgtgt gataagaga 
      ttttcgcgg aatgatctt ctattacgg cacaaaatt 
      gcacatgcg atgaaaatg gacgaccaa aaattcgaa 
      ttggaaaat ttcagcgat gatgatgaa gatccggat 
      gatgacacg attattatt aaaactgaa acggctttg 
      tatccatgt gctgaatgc aatctcatt tttattaag 
      aaagaggaa cttgatgca cacattcaa aagcatgaa 
      aaagggggt ggcgaagga attgtttgt aatgtttgt 
      atgaaaacg ttcagcaaa caagcgcat ttgaatcga 
      cataagaaa attcattcg cagaataag ccacatgtt 
      tgtccgacc tgtaataag ggatttgtg agggcggaa 
      cagcttaat aaccacatg aatatacat tctggaatc 
      aaaccgcat gtctgtgcg atttgtctc aaaggtttt 
      aaccaaata tcaaatttg aaagaccac atgcgaacc 
      cacaacggt gaaaagccc tttctttgt tccacctgt 
      ggcaagggc ttcaaccag ttgggaaac cttcgtcaa 
      cacaccatc cgccacagc ggcattaaa gcccacctt 
      tgcagtacg tgtggtaac ggtttcgcc agcaaaggt 
      gaactttca gcccatctc cgaaaacac accggtgcg 
      cgccctttc gtgtgtcca atctgtaac cacggcttt 
      acaacttca agttcgtta acaaaacac aaacgaatc 
      cattccggg gaaaaaccg tacgagtgt gacgtatgc 
      aaaatgaag ttttcgcgg tcggggatt ctagccaga 
      cataaaagg acgcacacg ggagagaaa ccttacgtt 
      tgcaagttt tgtacaaaa gcattttca cagtcgaat 
      gatctgacg tcacatttg cggattcac acgggggaa 
      aaaccgtac atttgcgat gtatgtgga caggcattt 
      aggcagagt tctgcgctt aaaactcat aagaaaacg 
      catcctgga cgagaggtt attgacacg aaaattggg 
      gtcttgggg gtcatgcat acccccttt ttaacgtaa 
      taaagtaag gggaaaagt tatgtttac caattagtt 
      ttttattta tttttttaa tatatctac caccttgta 
      attaattaa ttaattaat tttgccaac attttatca 
      actgttatt tattttaaa gcgttttgc tcaaaataa 
      aatccagat tagtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY