| General information | |||
| TC012996 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 15419138..15421176 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012996 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05367 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
beetle stock: bad (increased lethality),
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
- >TC012996 RA
MEEEISQKI CRACLTESG EFQSIFVPD EKTGTSIHI SEMMMAFAT VQVTLGDGL PEQICSTCA RTTVDMYMF KLKCEESEK ILRSRLAKS PFQEQFTIE EKHYVETKG DVDVYSIVI KPELCSEDS EINHELSSD FEHLNAPDK SDSDLDAFE LYADEGGQE HRCPHCPKV FDKESAANR HIKTHSAVK AYKCDTCDK RFSRNDLLL RHKIAHAMK MDDQKFELE NFSDDDEDP DDDTIIIKT ETALYPCAE CNLIFIKKE ELDAHIQKH EKGGGEGIV CNVCMKTFS KQAHLNRHK KIHSQNKPH VCPTCNKGF VRAEQLNNH MNIHSGIKP HVCAICLKG FNQISNLKD HMRTHNGEK PFLCSTCGK GFNQLGNLR QHTIRHSGI KAHLCSTCG NGFASKGEL SAHLRKHTG ARPFVCPIC NHGFTTSSS LTKHKRIHS GEKPYECDV CKMKFSRSG ILARHKRTH TGEKPYVCK FCTKAFSQS NDLTSHLRI HTGEKPYIC DVCGQAFRQ SSALKTHKK THPGREVID TKIGVLGVM HTPFLT
- >TC012996 RA
atggaggag gaaatctcc caaaaaatt tgtcgtgcc tgtttgacc gagtctggc gaattccag tcgatcttc gtccccgat gagaaaacc ggcacttcc atacacatt tctgaaatg atgatggca ttcgctacc gtccaggta actcttggt gacggtctc ccggaacaa atctgctcc acgtgcgcc cgcaccact gtagatatg tacatgttc aagttaaag tgcgaagaa tcggagaaa atcctgcga agtcggctg gccaaaagc cccttccag gagcagttc accatcgag gagaagcac tacgtggag accaagggg gacgtggac gtttacagc atagtgatc aagcctgag ctgtgctcc gaagactcg gaaatcaac cacgagctc agctcagac tttgagcat ttgaacgca ccggataaa agtgacagt gatttggac gcgtttgag ttgtacgcg gatgaaggg ggccaggag caccggtgt ccccattgc cccaaagtg ttcgataag gaaagtgcg gccaatcgc cacatcaaa acacattcg gctgttaaa gcttacaag tgtgatacg tgtgataag agattttcg cggaatgat cttctatta cggcacaaa attgcacat gcgatgaaa atggacgac caaaaattc gaattggaa aatttcagc gatgatgat gaagatccg gatgatgac acgattatt attaaaact gaaacggct ttgtatcca tgtgctgaa tgcaatctc atttttatt aagaaagag gaacttgat gcacacatt caaaagcat gaaaaaggg ggtggcgaa ggaattgtt tgtaatgtt tgtatgaaa acgttcagc aaacaagcg catttgaat cgacataag aaaattcat tcgcagaat aagccacat gtttgtccg acctgtaat aagggattt gtgagggcg gaacagctt aataaccac atgaatata cattctgga atcaaaccg catgtctgt gcgatttgt ctcaaaggt tttaaccaa atatcaaat ttgaaagac cacatgcga acccacaac ggtgaaaag ccctttctt tgttccacc tgtggcaag ggcttcaac cagttggga aaccttcgt caacacacc atccgccac agcggcatt aaagcccac ctttgcagt acgtgtggt aacggtttc gccagcaaa ggtgaactt tcagcccat ctccgaaaa cacaccggt gcgcgccct ttcgtgtgt ccaatctgt aaccacggc tttacaact tcaagttcg ttaacaaaa cacaaacga atccattcc ggggaaaaa ccgtacgag tgtgacgta tgcaaaatg aagttttcg cggtcgggg attctagcc agacataaa aggacgcac acgggagag aaaccttac gtttgcaag ttttgtaca aaagcattt tcacagtcg aatgatctg acgtcacat ttgcggatt cacacgggg gaaaaaccg tacatttgc gatgtatgt ggacaggca tttaggcag agttctgcg cttaaaact cataagaaa acgcatcct ggacgagag gttattgac acgaaaatt ggggtcttg ggggtcatg cataccccc tttttaacg taa
- >TC012996 RA
cgttcgatt gattgtcaa aagtttggt tgcaatttt aacctaata aaaataatg gaggaggaa atctcccaa aaaatttgt cgtgcctgt ttgaccgag tctggcgaa ttccagtcg atcttcgtc cccgatgag aaaaccggc acttccata cacatttct gaaatgatg atggcattc gctaccgtc caggtaact cttggtgac ggtctcccg gaacaaatc tgctccacg tgcgcccgc accactgta gatatgtac atgttcaag ttaaagtgc gaagaatcg gagaaaatc ctgcgaagt cggctggcc aaaagcccc ttccaggag cagttcacc atcgaggag aagcactac gtggagacc aagggggac gtggacgtt tacagcata gtgatcaag cctgagctg tgctccgaa gactcggaa atcaaccac gagctcagc tcagacttt gagcatttg aacgcaccg gataaaagt gacagtgat ttggacgcg tttgagttg tacgcggat gaagggggc caggagcac cggtgtccc cattgcccc aaagtgttc gataaggaa agtgcggcc aatcgccac atcaaaaca cattcggct gttaaagct tacaagtgt gatacgtgt gataagaga ttttcgcgg aatgatctt ctattacgg cacaaaatt gcacatgcg atgaaaatg gacgaccaa aaattcgaa ttggaaaat ttcagcgat gatgatgaa gatccggat gatgacacg attattatt aaaactgaa acggctttg tatccatgt gctgaatgc aatctcatt tttattaag aaagaggaa cttgatgca cacattcaa aagcatgaa aaagggggt ggcgaagga attgtttgt aatgtttgt atgaaaacg ttcagcaaa caagcgcat ttgaatcga cataagaaa attcattcg cagaataag ccacatgtt tgtccgacc tgtaataag ggatttgtg agggcggaa cagcttaat aaccacatg aatatacat tctggaatc aaaccgcat gtctgtgcg atttgtctc aaaggtttt aaccaaata tcaaatttg aaagaccac atgcgaacc cacaacggt gaaaagccc tttctttgt tccacctgt ggcaagggc ttcaaccag ttgggaaac cttcgtcaa cacaccatc cgccacagc ggcattaaa gcccacctt tgcagtacg tgtggtaac ggtttcgcc agcaaaggt gaactttca gcccatctc cgaaaacac accggtgcg cgccctttc gtgtgtcca atctgtaac cacggcttt acaacttca agttcgtta acaaaacac aaacgaatc cattccggg gaaaaaccg tacgagtgt gacgtatgc aaaatgaag ttttcgcgg tcggggatt ctagccaga cataaaagg acgcacacg ggagagaaa ccttacgtt tgcaagttt tgtacaaaa gcattttca cagtcgaat gatctgacg tcacatttg cggattcac acgggggaa aaaccgtac atttgcgat gtatgtgga caggcattt aggcagagt tctgcgctt aaaactcat aagaaaacg catcctgga cgagaggtt attgacacg aaaattggg gtcttgggg gtcatgcat acccccttt ttaacgtaa taaagtaag gggaaaagt tatgtttac caattagtt ttttattta tttttttaa tatatctac caccttgta attaattaa ttaattaat tttgccaac attttatca actgttatt tattttaaa gcgttttgc tcaaaataa aatccagat tagtta
