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Gene information
General information
TC012995 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15424272..15426256
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012995
    iBeetle screen iB_05366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012995 RA
      MSKMKIRMR LINVNKVCR TCLSERGHL REIFSTKLP 
      KMMENCASI QVCENDELP PHVCITCFH LIAKFYTFK 
      KKMERSDRI LRTYLKQNK MKQKDILDI AISEAQIEQ 
      SNNDDLGDI SEEDIPLSK RKVKKSPRL LRQSKVKAN 
      KIIDDFIKD NSDYSIEFV KVENDQQPI ITEGPPPLV 
      PLEPTKKID STETPITKA LPDDNPPPL IPIKPIISN 
      VIENALEIT VNCTNCNQQ FNSLTALKD HKLNDCKQN 
      NLKCNICRK EFKERKRLI GHLKGHMVV KDYGCKICG 
      KRYPNPSTF RVHMRTHTG ERPFKCQIC NKGFVRWAG 
      VVGHMRTHQ SHKPYKCET CGKGFKISS NLERHKVLH 
      SESLPYCCN YCGKTFSQS DNLQLHIRT YHTNERPYL 
      CNECGKGFV SSTRLNRHM WVHTGYKPY RCKACPKAY 
      SNSNDLKNH ERYHLGNSN EGDKPYVCK VCEMRFYHP 
      CRLNKHMKT HERPFSCSI CFKSFNSNA LLTKHLTGK 
      KSVHLVQVE EVQQDNEGV NQIFLDY
    CDS sequence
    • >TC012995 RA
      atgtcgaag atgaagata aggatgaga ctaatcaat 
      gtgaataaa gtgtgcagg acttgttta agtgaacgc 
      ggccacttg agggaaatt ttctcgaca aaattgcca 
      aagatgatg gaaaattgt gcttcaata caggtttgt 
      gagaacgat gagcttccg ccccacgtc tgcatcacc 
      tgttttcac ttaatcgca aagttttac acattcaag 
      aagaaaatg gaacggagc gatcgaatt ttaaggacg 
      tacttgaaa cagaacaaa atgaaacaa aaggatatt 
      ttagacatt gccataagt gaagcccag attgaacaa 
      tccaataat gacgatctt ggcgatatt agtgaagaa 
      gacattccg ttaagtaag cgtaaggtg aagaaaagc 
      ccacgtttg ttacgtcaa agtaaagta aaagcgaac 
      aaaataatt gacgatttt ataaaagac aattcggac 
      tattccatc gagtttgtt aaagtcgaa aacgaccaa 
      caaccaatc atcactgag ggaccccca cctctagtc 
      ccgcttgaa ccaaccaaa aaaatcgac agtaccgaa 
      acaccaatc acgaaagcg ctaccagac gacaacccc 
      ccaccacta atccccatc aaaccaatc atttcaaac 
      gtaatagaa aacgccctc gaaatcaca gtcaattgt 
      accaactgc aaccaacaa ttcaattcc ctaaccgcg 
      ttaaaagac cacaaactg aacgactgc aaacaaaac 
      aacctcaaa tgcaacata tgtcgcaag gagttcaaa 
      gagcgcaaa cgcctgatt ggccacttg aagggccac 
      atggtcgtg aaagactac ggttgcaaa atctgtggt 
      aagcgatat ccgaaccct agtacattt cgagtgcac 
      atgcgcaca catacgggc gaaaggccc ttcaagtgc 
      caaatttgc aataagggt ttcgtgcga tgggccggg 
      gttgtaggg cacatgcgc acccaccaa tcacacaag 
      ccctacaag tgcgaaact tgcggtaaa ggttttaaa 
      atatcgtcc aatttggaa cgccacaaa gtccttcat 
      tctgaaagt ttgccctac tgttgcaac tattgcggg 
      aagacattc agccaatcg gataatttg caattacac 
      attcggacc tatcacacc aacgaacgg ccctacctt 
      tgcaacgag tgcgggaaa gggttcgtc agctccact 
      aggttgaat agacatatg tgggtgcat acgggttat 
      aagccgtat cgttgtaaa gcttgtccg aaggcctat 
      tcgaattca aacgatttg aaaaatcat gagaggtac 
      catttgggg aatagcaac gaaggggat aagccctac 
      gtgtgtaaa gtgtgcgag atgaggttt tatcatcct 
      tgtaggctg aataaacat atgaagacg catgaaaga 
      ccattttcg tgctcgatt tgtttcaag agttttaat 
      tcgaatgcg ttgttgacg aaacatttg acgggaaaa 
      aaaagtgtg catttagtg caagtggaa gaggtgcag 
      caggataat gagggtgtt aatcaaatt ttcttggat 
      tattga
    mRNA sequence
    • >TC012995 RA
      tgacaacag ctgatgtga tggttgtcc acaaagcag 
      ttccaaatt aattccaca aaaatgtcg aagatgaag 
      ataaggatg agactaatc aatgtgaat aaagtgtgc 
      aggacttgt ttaagtgaa cgcggccac ttgagggaa 
      attttctcg acaaaattg ccaaagatg atggaaaat 
      tgtgcttca atacaggtt tgtgagaac gatgagctt 
      ccgccccac gtctgcatc acctgtttt cacttaatc 
      gcaaagttt tacacattc aagaagaaa atggaacgg 
      agcgatcga attttaagg acgtacttg aaacagaac 
      aaaatgaaa caaaaggat attttagac attgccata 
      agtgaagcc cagattgaa caatccaat aatgacgat 
      cttggcgat attagtgaa gaagacatt ccgttaagt 
      aagcgtaag gtgaagaaa agcccacgt ttgttacgt 
      caaagtaaa gtaaaagcg aacaaaata attgacgat 
      tttataaaa gacaattcg gactattcc atcgagttt 
      gttaaagtc gaaaacgac caacaacca atcatcact 
      gagggaccc ccacctcta gtcccgctt gaaccaacc 
      aaaaaaatc gacagtacc gaaacacca atcacgaaa 
      gcgctacca gacgacaac cccccacca ctaatcccc 
      atcaaacca atcatttca aacgtaata gaaaacgcc 
      ctcgaaatc acagtcaat tgtaccaac tgcaaccaa 
      caattcaat tccctaacc gcgttaaaa gaccacaaa 
      ctgaacgac tgcaaacaa aacaacctc aaatgcaac 
      atatgtcgc aaggagttc aaagagcgc aaacgcctg 
      attggccac ttgaagggc cacatggtc gtgaaagac 
      tacggttgc aaaatctgt ggtaagcga tatccgaac 
      cctagtaca tttcgagtg cacatgcgc acacatacg 
      ggcgaaagg cccttcaag tgccaaatt tgcaataag 
      ggtttcgtg cgatgggcc ggggttgta gggcacatg 
      cgcacccac caatcacac aagccctac aagtgcgaa 
      acttgcggt aaaggtttt aaaatatcg tccaatttg 
      gaacgccac aaagtcctt cattctgaa agtttgccc 
      tactgttgc aactattgc gggaagaca ttcagccaa 
      tcggataat ttgcaatta cacattcgg acctatcac 
      accaacgaa cggccctac ctttgcaac gagtgcggg 
      aaagggttc gtcagctcc actaggttg aatagacat 
      atgtgggtg catacgggt tataagccg tatcgttgt 
      aaagcttgt ccgaaggcc tattcgaat tcaaacgat 
      ttgaaaaat catgagagg taccatttg gggaatagc 
      aacgaaggg gataagccc tacgtgtgt aaagtgtgc 
      gagatgagg ttttatcat ccttgtagg ctgaataaa 
      catatgaag acgcatgaa agaccattt tcgtgctcg 
      atttgtttc aagagtttt aattcgaat gcgttgttg 
      acgaaacat ttgacggga aaaaaaagt gtgcattta 
      gtgcaagtg gaagaggtg cagcaggat aatgagggt 
      gttaatcaa attttcttg gattattga acaatttag 
      ttatttgct cgaatgaaa atagaaatg ttagcgtaa 
      cgatgtaaa aaaaaattt acatttcaa aattgcatt 
      gtgggtaat gtgattgtc gacacaatt gtaattaat 
      tgaagggtc gtagaaaaa acattgtgt tgtactcgt 
      tagaaaagc ttttttcat gctcctagc ggcctcgtg 
      catgaaaaa tacgctttt caacacttg caaaacaaa 
      ctaatattt tacactgta attctcagt tttcttata 
      tttaaagat aaataaata aataagtaa ttaaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY