Status
Please wait ...
Gene information
General information
TC031695
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012905,TC012906
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451215.1: 3621..21693
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031695
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05356

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031695 RA
      MALELSSHP PMPLDYSNG GTRENSTSP VNVSDSAPS 
      SPPGSSAFT VVTPKGRDL LPDRNGSPV QFPSPTGFA 
      SYCRTLWGS PFISSSPRY LSLAQNHSE QSTDKSKEP 
      INFGISRLV TKSEDEDEQ TNNNNNNNA KYESSDEMK 
      LNRWDNSPR PSPSTSPEL EVDSPPHSR TNSPSPRPP 
      SVTELNKSE TSKKSEAFS VSALLRPDN PKKSDGPCY 
      QETISVTRS YLYPPFVDL LKDAQLKSN PDYGSAFLP 
      RNFVHPGFF PPGLYSQKE SEERGFLPT PTSLYFSAV 
      AAAMANGQS QPGQYPPPP TSEELFRLR HHLMNSPGV 
      HPHHHHLLM RPGMMPLGD VYSCIKCEK MFSTPHGLE 
      VHARRSHNG KRPFACELC NKTFGHEIS LSQHRAVHN 
      VEKVFECKQ CGKSFKRSS TLSTHLLIH SDTRPYPCQ 
      YCGKRFHQK SDMKKHTYI HTGEKPHKC TVCGKAFSQ 
      SSNLITHSR KHTGFKPFA CDLCGRAFQ RKVDLRRHK 
      ETQHTELRP IVT
    CDS sequence
    • >TC031695 RA
      atggctttg gagctgagt tcacatcct cccatgccg 
      ttggactat agcaatggg gggacgaga gaaaattcg 
      acaagtccg gtgaatgtt tccgattcc gctccgtca 
      agtccgccg ggaagtagc gccttcacc gtcgtgacg 
      cccaaagga agagatctc ctgccggac cgaaacgga 
      tctcctgtt cagtttcct tctccgaca ggatttgcg 
      agctactgc agaacgctc tggggctca cccttcatt 
      tcaagcagc cctcgttat ttatctctc gctcaaaac 
      cattccgaa caatcaacc gacaagtcc aaagaacca 
      atcaacttc ggcatcagt cgactagtc acgaaatcc 
      gaagacgaa gacgaacaa accaacaac aacaacaac 
      aacaacgca aaatacgaa agtagcgac gaaatgaaa 
      ctaaatcga tgggacaac tcacccaga ccctcaccc 
      agcacttcg ccagagctg gaagtggac tctcctcca 
      cactcccga actaactcg ccttcgcct cggcctcct 
      tcagtcacc gagttgaac aaaagcgag acttcgaag 
      aaatccgaa gcgttttcg gtcagtgct ttactccga 
      cctgacaat ccaaaaaaa tcagacggg ccttgttac 
      caagaaacc atatcagta actcgatca tatctctat 
      ccaccgttc gttgattta ttaaaagac gcccaactt 
      aaatcaaac ccagactat ggctccgca ttccttccg 
      agaaacttt gtacatccc ggatttttc ccacccgga 
      ctctactca caaaaagag agcgaggaa cgaggcttt 
      ttgcccacc cccacaagt ttgtacttt agtgcagtt 
      gctgcagct atggcaaat ggacagtcg cagcctgga 
      caataccct ccaccaccc acttcggag gagctgttt 
      aggttaagg caccacctt atgaatagt ccaggggtt 
      catccgcac catcatcat ctgttgatg aggcccgga 
      atgatgcct ctgggggac gtttattcg tgtattaag 
      tgcgagaaa atgttttcg acacctcat ggacttgag 
      gtccacgcc aggaggtcg cataatggg aaaagaccg 
      ttcgcttgc gagctgtgc aataagacg ttcggacac 
      gaaatcagc ctcagccaa cacagagct gtccataat 
      gtggaaaaa gtattcgaa tgcaaacag tgcggtaaa 
      tcgtttaaa agatcttcg actctctcg actcatctc 
      cttatacat tccgatact cggccgtac ccttgccaa 
      tactgcggc aagaggttt catcagaag tcagacatg 
      aagaagcac acttatatt cataccggc gagaagccg 
      cacaagtgc accgtctgc ggcaaggcc ttcagccaa 
      tcctccaac ctcatcaca cactcccgg aagcacact 
      ggcttcaag cccttcgcc tgtgacctc tgtggacgg 
      gctttccag cgcaaggtg gatttgcga cggcacaaa 
      gagacccag cacacggag ttgaggccc atcgtcaca 
      tag
    mRNA sequence
    • >TC031695 RA
      aaagattaa actcaagct tatcaactc aaatacacc 
      agtatcaaa cagttgact gtcgactga atcttcgcc 
      aacacgcgt taccacaga taagctcca aaactgata 
      aaaaatcgt cgctcgttt ttggcctaa aaattaatt 
      ttgccggtt tcgatgcgg gtgtgatga gcttttgag 
      aatgtgtga tgtttattc tgaaagcaa ctacagatg 
      atgtgtttt tgtgatatt tttgcgatt tgatttttt 
      tttttttaa tggctttgg agctgagtt cacatcctc 
      ccatgccgt tggactata gcaatgggg ggacgagag 
      aaaattcga caagtccgg tgaatgttt ccgattccg 
      ctccgtcaa gtccgccgg gaagtagcg ccttcaccg 
      tcgtgacgc ccaaaggaa gagatctcc tgccggacc 
      gaaacggat ctcctgttc agtttcctt ctccgacag 
      gatttgcga gctactgca gaacgctct ggggctcac 
      ccttcattt caagcagcc ctcgttatt tatctctcg 
      ctcaaaacc attccgaac aatcaaccg acaagtcca 
      aagaaccaa tcaacttcg gcatcagtc gactagtca 
      cgaaatccg aagacgaag acgaacaaa ccaacaaca 
      acaacaaca acaacgcaa aatacgaaa gtagcgacg 
      aaatgaaac taaatcgat gggacaact cacccagac 
      cctcaccca gcacttcgc cagagctgg aagtggact 
      ctcctccac actcccgaa ctaactcgc cttcgcctc 
      ggcctcctt cagtcaccg agttgaaca aaagcgaga 
      cttcgaaga aatccgaag cgttttcgg tcagtgctt 
      tactccgac ctgacaatc caaaaaaat cagacgggc 
      cttgttacc aagaaacca tatcagtaa ctcgatcat 
      atctctatc caccgttcg ttgatttat taaaagacg 
      cccaactta aatcaaacc cagactatg gctccgcat 
      tccttccga gaaactttg tacatcccg gatttttcc 
      cacccggac tctactcac aaaaagaga gcgaggaac 
      gaggctttt tgcccaccc ccacaagtt tgtacttta 
      gtgcagttg ctgcagcta tggcaaatg gacagtcgc 
      agcctggac aataccctc caccaccca cttcggagg 
      agctgttta ggttaaggc accacctta tgaatagtc 
      caggggttc atccgcacc atcatcatc tgttgatga 
      ggcccggaa tgatgcctc tgggggacg tttattcgt 
      gtattaagt gcgagaaaa tgttttcga cacctcatg 
      gacttgagg tccacgcca ggaggtcgc ataatggga 
      aaagaccgt tcgcttgcg agctgtgca ataagacgt 
      tcggacacg aaatcagcc tcagccaac acagagctg 
      tccataatg tggaaaaag tattcgaat gcaaacagt 
      gcggtaaat cgtttaaaa gatcttcga ctctctcga 
      ctcatctcc ttatacatt ccgatactc ggccgtacc 
      cttgccaat actgcggca agaggtttc atcagaagt 
      cagacatga agaagcaca cttatattc ataccggcg 
      agaagccgc acaagtgca ccgtctgcg gcaaggcct 
      tcagccaat cctccaacc tcatcacac actcccgga 
      agcacactg gcttcaagc ccttcgcct gtgacctct 
      gtggacggg ctttccagc gcaaggtgg atttgcgac 
      ggcacaaag agacccagc acacggagt tgaggccca 
      tcgtcacat agtgccaaa gcgccggaa gcagtgttg 
      cgcttccgg ccaaggagg acgaaggag gcaagttgt 
      tcaggccgc attcaacgc taatggacc aaaatgcgt 
      cggcacatt tgatcagcg ctgaatcgg cgccgcaac 
      tgccgccga tacgttcag tgttttcaa atgtttgtt 
      ggacgtcgt aaacatacc aaagactta ccaaattca 
      ctctaggct ttcatctgt gattttttg acatcatat 
      gagatatgg tgctagttt tggactact tttgtattt 
      gttgcgaga ccttttgga atcggattc cgggaggga 
      gaagcaccg acttgggtc acactgtac taggtgtta 
      ttacaaata tttaatatc taacgtgca atttgttgt 
      ttcatagtg caattttac agctattaa tgacgatgg 
      aaacatatc agaataact ggcattatt ttcagcact 
      ttacatttt cttaattaa taaagtcca agatttttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY