| General information | |||
| TC012902 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451215.1: 63301..67392 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012902 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05355 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
| about 25 |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC012902 RA
MNGSFELLS TMSPKVNKS IINSNLVPP CKKQTSLQA RPTKLALRK YFRKSQNAR HVKQSIKHD LIIEIESSS SCSFEEKVL NETPIVISD SDNSFIIEK TPVKTVPEK DCNKRHMPG QSNERMSKG KYNSIELWV EDVNKAKHN VSGFSEISS MIGNDNPGF KKSNINDAA SSTMLESNL RFDELFRKK NINQENDLT VNNSLKSEN QSLENVFKN MILNDSLYL NESFTSRLK NKNCVADNN KNSQRNGKN DTQIGSSQL DTNKSHNTS PGREKAENM GASDCEEYE KLLDSLYGN SWRDKREDI LPSSEPKLK KNKLKITKA VPNTERREN KFSNLLDER KESNLLKDL QRARFRQAL ESPLMETIK KLCDSDTDS EDENKMPHV KLNFDDESS ESDCVVPTK SPIKKTQLL KENNENFLG TCSFLASLS GCVPATNCD MTALIFRNN FKQQRDKLT EKLFHLYNE QVFDNSISE DTRIEWSEK LRGTAGYCR CRKITKRTG VVERHATIV LSSKVLDRA DRLRDTLIH EMCHAATWI VNQVTDGHG PFWKSWASR AMKTFPELP PIKRCHEYV INTKYTYKC TGCGYSIGR HTKSLNTET KRCGYCYGK FEVLINKIS KKGETKSIP ATPKKPSGF ALFVKENYA TVKTPDLKH GDVMKILGQ QFADLKVDQ S
- >TC012902 RB
MSKGKYNSI ELWVEDVNK AKHNVSGFS EISSMIGND NPGFKKSNI NDAASSTML ESNLRFDEL FRKKNINQE NDLTVNNSL KSENQSLEN VFKNMILND SLYLNESFT SRLKNKNCV ADNNKNSQR NGKNDTQIG SSQLDTNKS HNTSPGREK AENMGASDC EEYEKLLDS LYGNSWRDK REDILPSSE PKLKKNKLK ITKAVPNTE RRENKFSNL LDERKESNL LKDLQRARF RQALESPLM ETIKKLCDS DTDSEDENK MPHVKLNFD DESSESDCV VPTKSPIKK TQLLKENNE NFLGTCSFL ASLSGCVPA TNCDMTALI FRNNFKQQR DKLTEKLFH LYNEQVFDN SISEDTRIE WSEKLRGTA GYCRCRKIT KRTGVVERH ATIVLSSKV LDRADRLRD TLIHEMCHA ATWIVNQVT DGHGPFWKS WASRAMKTF PELPPIKRC HEYVINTKY TYKCTGCGY SIGRHTKSL NTETKRCGY CYGKFEVLI NKISKKGET KSIPATPKK PSGFALFVK ENYATVKTP DLKHGDVMK ILGQQFADL KVDQS
- >TC012902 RA
atgaacgga tctttcgag ctgttaagt acaatgtcc cctaaagtt aataaatca attataaac agtaatctc gtacctcca tgcaagaag caaacatct ttacaagca cgtccaact aaattggct ttaaggaaa tactttaga aaatcgcaa aatgcacgc cacgtaaaa caatcaata aaacatgat ttgataatt gaaatagag tctagtagc agttgctct tttgaagaa aaggttttg aacgagaca cctattgta atatcggac agtgataat tcttttata attgaaaaa acaccggtt aaaactgta cctgaaaaa gattgcaat aaaagacac atgccagga caaagtaat gaaagaatg tcaaaagga aaatacaat agtattgag ctttgggtt gaagatgtt aataaagct aagcacaac gtctctggt ttcagcgaa atatcttca atgattgga aatgataat cccggcttt aaaaaaagc aatataaat gatgctgcg agttcaacg atgttagaa tctaattta cgttttgat gagttgttt aggaaaaaa aatattaat caggaaaat gatttgacg gtgaataac agtttgaaa agtgaaaat cagagcctg gaaaatgta tttaaaaat atgattttg aatgattct ctctatttg aatgaaagt tttacttca aggcttaaa aataaaaat tgtgttgct gataacaac aaaaactca caacgcaat ggaaaaaat gacacccag attggatcc tcacagtta gatacaaat aaaagtcac aatacaagc ccaggaaga gagaaagcc gaaaatatg ggtgcttcc gattgtgag gagtatgag aagttgtta gacagttta tatggtaac agttggaga gataaaaga gaagatatt ctgccttca tcagagccc aaattaaaa aaaaacaaa ttgaaaatt actaaagcg gttccaaac actgaaaga cgagaaaat aaattttcg aatttacta gatgaaaga aaggaatca aacttatta aaagattta caaagagca cgatttaga caagcttta gaatcgcct ttgatggaa actattaaa aaactttgt gactctgac acagattca gaggatgaa aataaaatg cctcacgtg aaattaaat tttgatgat gaatcatct gaatctgat tgtgttgta ccaacaaaa tcaccaatc aaaaaaacg caattgcta aaagagaat aatgaaaat tttcttggc acttgtagc tttttagcg tcactgtct ggatgtgtt ccagctaca aattgtgat atgactgcc ctaattttt agaaataat tttaaacaa cagagagac aaactgact gaaaaacta tttcatttg tataatgaa caagttttt gacaattcc atttcagaa gatacaaga atcgaatgg agcgaaaag ttgagaggc acagctgga tattgtcgt tgtagaaaa attaccaaa aggacgggt gttgtcgaa agacacgct actatagtt ttatctagt aaagtacta gacagggct gacagattg cgagatact ttgatacac gaaatgtgc catgcggct acgtggatt gttaatcaa gtaaccgat ggacatggt ccgttttgg aaatcctgg gcttcgcgt gcaatgaaa acttttccc gaacttccg ccaattaaa agatgccac gaatacgtc attaatact aaatatact tataaatgc accggttgt ggatacagt attgggcgg cacacaaag tctttaaat acagaaacg aaacgttgt ggctattgc tatggaaag tttgaggtt ttaattaat aagattagt aaaaaaggt gaaacaaaa tcgatccct gccacacct aagaagccg tcaggattt gcattgttt gttaaagaa aattatgca actgtgaaa acgcctgac ttgaaacat ggcgatgtt atgaaaatt ttgggacaa caatttgcc gacttaaaa gttgaccaa tcttaa
- >TC012902 RB
atgtcaaaa ggaaaatac aatagtatt gagctttgg gttgaagat gttaataaa gctaagcac aacgtctct ggtttcagc gaaatatct tcaatgatt ggaaatgat aatcccggc tttaaaaaa agcaatata aatgatgct gcgagttca acgatgtta gaatctaat ttacgtttt gatgagttg tttaggaaa aaaaatatt aatcaggaa aatgatttg acggtgaat aacagtttg aaaagtgaa aatcagagc ctggaaaat gtatttaaa aatatgatt ttgaatgat tctctctat ttgaatgaa agttttact tcaaggctt aaaaataaa aattgtgtt gctgataac aacaaaaac tcacaacgc aatggaaaa aatgacacc cagattgga tcctcacag ttagataca aataaaagt cacaataca agcccagga agagagaaa gccgaaaat atgggtgct tccgattgt gaggagtat gagaagttg ttagacagt ttatatggt aacagttgg agagataaa agagaagat attctgcct tcatcagag cccaaatta aaaaaaaac aaattgaaa attactaaa gcggttcca aacactgaa agacgagaa aataaattt tcgaattta ctagatgaa agaaaggaa tcaaactta ttaaaagat ttacaaaga gcacgattt agacaagct ttagaatcg cctttgatg gaaactatt aaaaaactt tgtgactct gacacagat tcagaggat gaaaataaa atgcctcac gtgaaatta aattttgat gatgaatca tctgaatct gattgtgtt gtaccaaca aaatcacca atcaaaaaa acgcaattg ctaaaagag aataatgaa aattttctt ggcacttgt agcttttta gcgtcactg tctggatgt gttccagct acaaattgt gatatgact gccctaatt tttagaaat aattttaaa caacagaga gacaaactg actgaaaaa ctatttcat ttgtataat gaacaagtt tttgacaat tccatttca gaagataca agaatcgaa tggagcgaa aagttgaga ggcacagct ggatattgt cgttgtaga aaaattacc aaaaggacg ggtgttgtc gaaagacac gctactata gttttatct agtaaagta ctagacagg gctgacaga ttgcgagat actttgata cacgaaatg tgccatgcg gctacgtgg attgttaat caagtaacc gatggacat ggtccgttt tggaaatcc tgggcttcg cgtgcaatg aaaactttt cccgaactt ccgccaatt aaaagatgc cacgaatac gtcattaat actaaatat acttataaa tgcaccggt tgtggatac agtattggg cggcacaca aagtcttta aatacagaa acgaaacgt tgtggctat tgctatgga aagtttgag gttttaatt aataagatt agtaaaaaa ggtgaaaca aaatcgatc cctgccaca cctaagaag ccgtcagga tttgcattg tttgttaaa gaaaattat gcaactgtg aaaacgcct gacttgaaa catggcgat gttatgaaa attttggga caacaattt gccgactta aaagttgac caatcttaa
- >TC012902 RA
caacaatct gtcgttaac tgtcactgt cttatattt taaaatatt gttttttta tacttttta ccgtatttt tcaatctac aaattttta ccctactat gaacggatc tttcgagct gttaagtac aatgtcccc taaagttaa taaatcaat tataaacag taatctcgt acctccatg caagaagca aacatcttt acaagcacg tccaactaa attggcttt aaggaaata ctttagaaa atcgcaaaa tgcacgcca cgtaaaaca atcaataaa acatgattt gataattga aatagagtc tagtagcag ttgctcttt tgaagaaaa ggttttgaa cgagacacc tattgtaat atcggacag tgataattc ttttataat tgaaaaaac accggttaa aactgtacc tgaaaaaga ttgcaataa aagacacat gccaggaca aagtaatga aagaatgtc aaaaggaaa atacaatag tattgagct ttgggttga agatgttaa taaagctaa gcacaacgt ctctggttt cagcgaaat atcttcaat gattggaaa tgataatcc cggctttaa aaaaagcaa tataaatga tgctgcgag ttcaacgat gttagaatc taatttacg ttttgatga gttgtttag gaaaaaaaa tattaatca ggaaaatga tttgacggt gaataacag tttgaaaag tgaaaatca gagcctgga aaatgtatt taaaaatat gattttgaa tgattctct ctatttgaa tgaaagttt tacttcaag gcttaaaaa taaaaattg tgttgctga taacaacaa aaactcaca acgcaatgg aaaaaatga cacccagat tggatcctc acagttaga tacaaataa aagtcacaa tacaagccc aggaagaga gaaagccga aaatatggg tgcttccga ttgtgagga gtatgagaa gttgttaga cagtttata tggtaacag ttggagaga taaaagaga agatattct gccttcatc agagcccaa attaaaaaa aaacaaatt gaaaattac taaagcggt tccaaacac tgaaagacg agaaaataa attttcgaa tttactaga tgaaagaaa ggaatcaaa cttattaaa agatttaca aagagcacg atttagaca agctttaga atcgccttt gatggaaac tattaaaaa actttgtga ctctgacac agattcaga ggatgaaaa taaaatgcc tcacgtgaa attaaattt tgatgatga atcatctga atctgattg tgttgtacc aacaaaatc accaatcaa aaaaacgca attgctaaa agagaataa tgaaaattt tcttggcac ttgtagctt tttagcgtc actgtctgg atgtgttcc agctacaaa ttgtgatat gactgccct aatttttag aaataattt taaacaaca gagagacaa actgactga aaaactatt tcatttgta taatgaaca agtttttga caattccat ttcagaaga tacaagaat cgaatggag cgaaaagtt gagaggcac agctggata ttgtcgttg tagaaaaat taccaaaag gacgggtgt tgtcgaaag acacgctac tatagtttt atctagtaa agtactaga cagggctga cagattgcg agatacttt gatacacga aatgtgcca tgcggctac gtggattgt taatcaagt aaccgatgg acatggtcc gttttggaa atcctgggc ttcgcgtgc aatgaaaac ttttcccga acttccgcc aattaaaag atgccacga atacgtcat taatactaa atatactta taaatgcac cggttgtgg atacagtat tgggcggca cacaaagtc tttaaatac agaaacgaa acgttgtgg ctattgcta tggaaagtt tgaggtttt aattaataa gattagtaa aaaaggtga aacaaaatc gatccctgc cacacctaa gaagccgtc aggatttgc attgtttgt taaagaaaa ttatgcaac tgtgaaaac gcctgactt gaaacatgg cgatgttat gaaaatttt gggacaaca atttgccga cttaaaagt tgaccaatc ttaattaat aatagttat gtatgaaat tttcgtttt taacaacat gaaactaaa atagcacaa actagacat gtggttttc ctgctccaa cagttgacc aatctttta tgtagatat acgttcttc gtgattctc caaggccaa ggacaatga aataatgat gattcattt ttgaaaaat taacattta tgatatgtt ctttgataa atatgatta taatattct gttttgtta agtcgttaa tcaatttat ttaaaaaaa acctaattt agataacac atcatattc tgcttctgt ctatagaga ctatcaaat aatcaacaa taagtaatt tgcagttga gtaaactcc atatgaatt tattatgat tcatcccat gtgtttaca ctttccttt ttataaatt ttaaattga aagttttgt ttatgaata tattgttat ttatttttt cttcataga cgacaattt tggttttgg aattatgaa atgtgtcat ttcataatt acattcttt ttttactct tgctaaatt tcttttact atttacaac aaaggctaa ctaatatcc atcaagtgt aaaaagttg aatatgttt gtgtttgca agttgacta atttgcact ttacatcag tttacttca atgaatcat ttccatgaa attaattaa ctcatctag attgttatc tctacaaag caaagttaa aacaatcca aaacccaat tacactctt tgaatcatc agaggctat gactgcaaa aagtaattc cgtttctgt gattacatc tgaatataa ttatataaa cctatcata ctgatgaat tcagcaaga agcagacag atatttgaa taagtaagc tcgtaaaaa aacagcaca ccgcaaatg gattgctta ttgaaattt tgaattgtt aaaaaatcc aactacaaa aaataaccg ttcacacat attacagaa acagagtgt caaaaaata gtactgtgt cttagttta cttcgcgat ctttttttc caatcacat attttgcaa aaccatgac tggaaccag ttttgggat ttaaaaaaa gcattttaa cttgattat tagataata tgtaaataa taagaatta tgtaatatt gaccatttt cttgaaaaa accccgtgt caaatggca aaattttat ttttttcct aattcaaaa tgttaaatg attcgtgac cttttttat tattcattt aaaacgata acatcgtaa atttttctt gttctccta ttaattccg ttttttatt gttagaacg actaaagac acaatttcc ataaaacaa caataatta ttattaaca atg
- >TC012902 RB
acatcttta caagcacgt aagtactgc acaaagtta tcattccga caactggta agagtgttt gtaggtcca actaaattg gctttaagg aaatacttt agaaaatcg caaaatgca cgccacgta aaacaatca ataaaacat gatttgata attgaaata gagtctagt agcagttgc tcttttgaa gaaaaggtt ttgaacgag acacctatt gtaatatcg gacagtgat aattctttt ataattgaa aaaacaccg gttaaaact gtacctgaa aaagattgc aataaaaga cacatgcca ggacaaagt aatgaaaga atgtcaaaa ggaaaatac aatagtatt gagctttgg gttgaagat gttaataaa gctaagcac aacgtctct ggtttcagc gaaatatct tcaatgatt ggaaatgat aatcccggc tttaaaaaa agcaatata aatgatgct gcgagttca acgatgtta gaatctaat ttacgtttt gatgagttg tttaggaaa aaaaatatt aatcaggaa aatgatttg acggtgaat aacagtttg aaaagtgaa aatcagagc ctggaaaat gtatttaaa aatatgatt ttgaatgat tctctctat ttgaatgaa agttttact tcaaggctt aaaaataaa aattgtgtt gctgataac aacaaaaac tcacaacgc aatggaaaa aatgacacc cagattgga tcctcacag ttagataca aataaaagt cacaataca agcccagga agagagaaa gccgaaaat atgggtgct tccgattgt gaggagtat gagaagttg ttagacagt ttatatggt aacagttgg agagataaa agagaagat attctgcct tcatcagag cccaaatta aaaaaaaac aaattgaaa attactaaa gcggttcca aacactgaa agacgagaa aataaattt tcgaattta ctagatgaa agaaaggaa tcaaactta ttaaaagat ttacaaaga gcacgattt agacaagct ttagaatcg cctttgatg gaaactatt aaaaaactt tgtgactct gacacagat tcagaggat gaaaataaa atgcctcac gtgaaatta aattttgat gatgaatca tctgaatct gattgtgtt gtaccaaca aaatcacca atcaaaaaa acgcaattg ctaaaagag aataatgaa aattttctt ggcacttgt agcttttta gcgtcactg tctggatgt gttccagct acaaattgt gatatgact gccctaatt tttagaaat aattttaaa caacagaga gacaaactg actgaaaaa ctatttcat ttgtataat gaacaagtt tttgacaat tccatttca gaagataca agaatcgaa tggagcgaa aagttgaga ggcacagct ggatattgt cgttgtaga aaaattacc aaaaggacg ggtgttgtc gaaagacac gctactata gttttatct agtaaagta ctagacagg gctgacaga ttgcgagat actttgata cacgaaatg tgccatgcg gctacgtgg attgttaat caagtaacc gatggacat ggtccgttt tggaaatcc tgggcttcg cgtgcaatg aaaactttt cccgaactt ccgccaatt aaaagatgc cacgaatac gtcattaat actaaatat acttataaa tgcaccggt tgtggatac agtattggg cggcacaca aagtcttta aatacagaa acgaaacgt tgtggctat tgctatgga aagtttgag gttttaatt aataagatt agtaaaaaa ggtgaaaca aaatcgatc cctgccaca cctaagaag ccgtcagga tttgcattg tttgttaaa gaaaattat gcaactgtg aaaacgcct gacttgaaa catggcgat gttatgaaa attttggga caacaattt gccgactta aaagttgac caatcttaa ttaataata gttatgtat gaaattttc gtttttaac aacatgaaa ctaaaatag cacaaacta gacatgtgg ttttcctgc tccaacagt tgaccaatc ttttatgta gatatacgt tcttcgtga ttctccaag gccaaggac aatgaaata atgatgatt catttttga aaaattaac atttatgat atgttcttt gataaatat gattataat attctgttt tgttaagtc gttaatcaa tttatttaa aaaaaacct aatttagat aacacatca tattctgct tctgtctat agagactat caaataatc aacaataag taatttgca gttgagtaa actccatat gaatttatt atgattcat cccatgtgt ttacacttt cctttttat aaattttaa attgaaagt tttgtttat gaatatatt gttatttat tttttcttc atagacgac aattttggt tttggaatt atgaaatgt gtcatttca taattacat tcttttttt actcttgct aaatttctt ttactattt acaacaaag gctaactaa tatccatca agtgtaaaa agttgaata tgtttgtgt ttgcaagtt gactaattt gcactttac atcagttta cttcaatga atcatttcc atgaaatta attaactca tctagattg ttatctcta caaagcaaa gttaaaaca atccaaaac ccaattaca ctctttgaa tcatcagag gctatgact gcaaaaagt aattccgtt tctgtgatt acatctgaa tataattat ataaaccta tcatactga tgaattcag caagaagca gacagatat ttgaataag taagctcgt aaaaaaaca gcacaccgc aaatggatt gcttattga aattttgaa ttgttaaaa aatccaact acaaaaaat aaccgttca cacatatta cagaaacag agtgtcaaa aaatagtac tgtgtctta gtttacttc gcgatcttt ttttccaat cacatattt tgcaaaacc atgactgga accagtttt gggatttaa aaaaagcat tttaacttg attattaga taatatgta aataataag aattatgta atattgacc attttcttg aaaaaaccc cgtgtcaaa tggcaaaat tttattttt ttcctaatt caaaatgtt aaatgattc gtgaccttt tttattatt catttaaaa cgataacat cgtaaattt ttcttgttc tcctattaa ttccgtttt ttattgtta gaacgacta aagacacaa tttccataa aacaacaat aattattat taacaatg
