Status
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Gene information
General information
TC034612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18659339..18681976
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034612
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034612 RA
      MEHRSDEIK LGGISKVSL PFYEPPPRK TSAEIINEA 
      RLAIRESQM AESSFISTP IKPLQTQRP FTPRNKERL 
      LFGKKTKAN RPPSSFSLR YLQNEAEIP PNVPESNMI 
      LSPHMLSSA SKTNTRPIF TKQRSNSLS EVNDNKIFN 
      IGFKEISLN KVKLPSLDH MKPLQKRKI FKNTSLDNL 
      PEEQESNSA DSKTERKAF SSPQERTDF NEDDSPFGR 
      SSSKQKNLS QCLLLGPQN LNKIFDNRH IIENSETLF 
      VGTENKERN IDDVIDDLN NESNRMDNE DFVIELLEE 
      LYGKMEKAN LLSTKVSSK MKIHVLKAL YKFVESQNE 
      RLLLNIARV ILGLKVTGN NLSGVCKLI FKVSKNDKN 
      DYLFFQKNI LELFLDALG RSSPLDDAE ACVYGYGSV 
      KFLTMNAKL LDKILSLGI LQLMVLHIK IINNAKMDK 
      TQMPEQTNH ALFQLTGAL RNIVSEETI YETFISCGA 
      IQQLCQTME LFSADLDII SNISRIFST ISTNDCCCD 
      SLVEYRDIY KVIIQLFDK YPGNEEIIV RLAYTLGNI 
      VAKIDNTRV KFYHEKNSL NSLLNLWKI YLERTLKIC 
      SLKTDNELI ENGNPEDVM IKVIRVIAN VVINPEIGK 
      AVNESFGCL LIDEFLKVL ISNPFKKNE ELVMSILST 
      LNNLSYYYT SDTETDIFH IKQVEIVEG ITEYAKSRN 
      KECVIESMR IMGNLSRSK ITRDYIAES EVFKILLNI 
      LDKADLTLL KTTIGVFVN LMADNKSRN LFKNQGGVA 
      KLITILKNY GQNDWLLGM LVCQVIWNY CIDTMDLYE 
      LIPDDEIQQ LLVILADYL DEEKLFGIT EGSEDTEVY 
      VTQEYLIWE EFASVATNL LEKIEYFLD TFDQIHIEN 
      EATLTKDSS TNLSFAAW
    • >TC034612 RB
      MEHRSDEIK LGGISKVSL PFYEPPPRK TSAEIINEA 
      RLAIRESQM AESSFISTP IKPLQTQRP FTPRNKERL 
      LFGKKTKAN RPPSSFSLR YLQNEAEIP PNVPESNMI 
      LSPHMLSSA SKTNTRPIF TKQRSNSLS EVNDNKIFN 
      IGFKEISLN KVKLPSLDH MKPLQKRKI FKNTSLDNL 
      PEEQESNSA DSKTERKAF SSPQERTDF NEDDSPFGR 
      SSSKQKNLS QCLLLGPQN LNKIFDNRH IIENSETLF 
      VGTGYLNRG QNPHRGNKV QNSILENKE RNIDDVIDD 
      LNNESNRMD NEDFVIELL EELYGKMEK ANLLSTKVS 
      SKMKIHVLK ALYKFVESQ NERLLLNIA RVILGLKVT 
      GNNLSGVCK LIFKVSKND KNDYLFFQK NILELFLDA 
      LGRSSPLDD AEACVYGYG SVKFLTMNA KLLDKILSL 
      GILQLMVLH IKIINNAKM DKTQMPEQT NHALFQLTG 
      ALRNIVSEE TIYETFISC GAIQQLCQT MELFSADLD 
      IISNISRIF STISTNDCC CDSLVEYRD IYKVIIQLF 
      DKYPGNEEI IVRLAYTLG NIVAKIDNT RVKFYHEKN 
      SLNSLLNLW KIYLERTLK ICSLKTDNE LIENGNPED 
      VMIKVIRVI ANVVINPEI GKAVNESFG CLLIDEFLK 
      VLISNPFKK NEELVMSIL STLNNLSYY YTSDTETDI 
      FHIKQVEIV EGITEYAKS RNKECVIES MRIMGNLSR 
      SKITRDYIA ESEVFKILL NILDKADLT LLKTTIGVF 
      VNLMADNKS RNLFKNQGG VAKLITILK NYGQNDWLL 
      GMLVCQVIW NYCIDTMDL YELIPDDEI QQLLVILAD 
      YLDEEKLFG ITEGSEDTE VYVTQEYLI WEEFASVAT 
      NLLEKIEYF LDTFDQIHI ENEATLTKD SSTNLSFAA 
      W
    CDS sequence
    • >TC034612 RA
      atggaacat agaagtgac gaaattaaa ttaggggga 
      ataagtaaa gtgtctttg ccattttat gaaccccca 
      ccaagaaaa accagtgcg gaaattatc aacgaagcg 
      cgccttgct ataagagaa tcacaaatg gctgagagc 
      tcctttatt tcaactcct atcaaacct ctgcaaacg 
      caaaggcct ttcacaccg agaaacaag gaaaggcta 
      ctgtttggc aaaaaaacc aaagcgaat cggccgcca 
      agttccttc agtctccga tacctccaa aatgaggcg 
      gaaatacca cccaacgtc cccgaatca aacatgatc 
      ctaagccct cacatgtta tcatcagcc tcaaaaacc 
      aacactcgg ccaattttc accaaacaa cgttcaaac 
      tcactatcc gaagtcaac gacaacaaa atcttcaat 
      atcggattc aaagaaatc tcactcaat aaagtcaaa 
      ctgccctca ctggaccac atgaaaccg ttgcaaaaa 
      cgcaaaatc ttcaaaaac acatctctc gacaatctg 
      cccgaagaa caagaatca aattctgcc gactcgaaa 
      accgaacgc aaagctttc agttctcca caagaacgc 
      acagatttc aacgaagat gattccccg tttggacgc 
      tcgtccagc aaacagaaa aatctctcg caatgtctt 
      ctcctaggt ccacaaaac ttgaacaaa atcttcgat 
      aatagacac attattgag aattctgag acgctgttt 
      gtggggaca gaaaataag gaacgaaac atcgacgat 
      gttattgac gatttaaat aatgagagc aatcgaatg 
      gacaacgaa gattttgtc attgaatta ttagaagaa 
      ctttatgga aaaatggaa aaagcgaat ttgctttcg 
      accaaagtc agttcgaaa atgaagatt cacgtgctg 
      aaagctttg tacaagttt gtggaatcg caaaatgag 
      cggcttttg ttgaacatt gccagggtt attttgggg 
      ctgaaggta actggcaac aatctcagt ggagtttgt 
      aaattgatt ttcaaagta tccaagaat gacaaaaac 
      gactactta ttcttccag aaaaatatt ttagaatta 
      tttttggac gctttgggc cgttcaagc cctttagac 
      gatgccgag gcttgcgtt tacggttac ggttccgtc 
      aaattttta acaatgaat gcgaaatta ctcgataaa 
      attttatca ctagggata ttacagttg atggtttta 
      cacatcaaa atcattaat aatgctaaa atggataaa 
      acacaaatg cctgaacaa accaatcat gctttattc 
      caactaacc ggtgcctta cgaaacata gtttcagaa 
      gaaactatt tatgaaaca ttcatttcg tgtggagct 
      attcaacag ttatgtcag accatggag cttttctcg 
      gcggatttg gatattatt agtaacata tcacgtatt 
      ttcagtaca atatcaacc aacgattgt tgttgcgat 
      agcttagtt gaatataga gatatttat aaagtgatt 
      attcaatta ttcgataaa tatcccgga aatgaggaa 
      attattgta cgtctggcg tacacttta ggaaacatt 
      gtcgccaaa attgataac actcgagtc aaattctat 
      cacgagaaa aattcgcta aacagtctc ctgaacttg 
      tggaagatt tatttagag cggacgctg aaaatctgc 
      tccctcaaa accgacaac gaattaatc gaaaatgga 
      aatccggaa gacgtcatg attaaagtg attcgagtc 
      attgcaaac gttgtcata aacccagaa atcggaaaa 
      gccgtaaat gagtccttt ggctgcctc ctcatcgac 
      gaattcctc aaagtccta atcagcaat cccttcaaa 
      aagaacgaa gaactcgtc atgtccatc ttgagtact 
      ttaaacaac ctatcgtac tattacacc tccgacaca 
      gaaacggac attttccac atcaagcaa gtggaaatc 
      gttgaggga atcaccgaa tatgcaaaa tcacgcaac 
      aaagaatgc gtaattgaa tcgatgaga atcatgggc 
      aatttatca cgctcgaaa attacgaga gattacatt 
      gcggaatcg gaagttttt aaaattctt ctaaatatt 
      ctagacaaa gccgatttg actctcctc aaaacgacg 
      atcggagta tttgttaat ttgatggca gacaataag 
      tcacgaaat ttgttcaag aatcaggga ggagttgcg 
      aaattgatc acgattttg aaaaactac ggacaaaat 
      gattggtta ttggggatg ttggtgtgt caagtgatt 
      tggaattat tgtattgat acgatggat ttatacgaa 
      cttatccct gatgatgag attcaacaa ttgctcgtg 
      attttggcc gattatctc gatgaagaa aaattgttc 
      ggaatcacg gaagggtcc gaagatact gaagtatac 
      gtaacccag gagtactta atttgggag gagtttgcc 
      agcgtcgcg actaattta ctggaaaaa atcgaatac 
      tttttggac acttttgac caaattcat attgaaaac 
      gaagccaca cttactaag gattcgagt acaaatctc 
      agtttcgca gcttggtaa
    • >TC034612 RB
      atggaacat agaagtgac gaaattaaa ttaggggga 
      ataagtaaa gtgtctttg ccattttat gaaccccca 
      ccaagaaaa accagtgcg gaaattatc aacgaagcg 
      cgccttgct ataagagaa tcacaaatg gctgagagc 
      tcctttatt tcaactcct atcaaacct ctgcaaacg 
      caaaggcct ttcacaccg agaaacaag gaaaggcta 
      ctgtttggc aaaaaaacc aaagcgaat cggccgcca 
      agttccttc agtctccga tacctccaa aatgaggcg 
      gaaatacca cccaacgtc cccgaatca aacatgatc 
      ctaagccct cacatgtta tcatcagcc tcaaaaacc 
      aacactcgg ccaattttc accaaacaa cgttcaaac 
      tcactatcc gaagtcaac gacaacaaa atcttcaat 
      atcggattc aaagaaatc tcactcaat aaagtcaaa 
      ctgccctca ctggaccac atgaaaccg ttgcaaaaa 
      cgcaaaatc ttcaaaaac acatctctc gacaatctg 
      cccgaagaa caagaatca aattctgcc gactcgaaa 
      accgaacgc aaagctttc agttctcca caagaacgc 
      acagatttc aacgaagat gattccccg tttggacgc 
      tcgtccagc aaacagaaa aatctctcg caatgtctt 
      ctcctaggt ccacaaaac ttgaacaaa atcttcgat 
      aatagacac attattgag aattctgag acgctgttt 
      gtggggaca ggttacttg aataggggg caaaatcca 
      caccgtgga aataaagta caaaattca attttagaa 
      aataaggaa cgaaacatc gacgatgtt attgacgat 
      ttaaataat gagagcaat cgaatggac aacgaagat 
      tttgtcatt gaattatta gaagaactt tatggaaaa 
      atggaaaaa gcgaatttg ctttcgacc aaagtcagt 
      tcgaaaatg aagattcac gtgctgaaa gctttgtac 
      aagtttgtg gaatcgcaa aatgagcgg cttttgttg 
      aacattgcc agggttatt ttggggctg aaggtaact 
      ggcaacaat ctcagtgga gtttgtaaa ttgattttc 
      aaagtatcc aagaatgac aaaaacgac tacttattc 
      ttccagaaa aatatttta gaattattt ttggacgct 
      ttgggccgt tcaagccct ttagacgat gccgaggct 
      tgcgtttac ggttacggt tccgtcaaa tttttaaca 
      atgaatgcg aaattactc gataaaatt ttatcacta 
      gggatatta cagttgatg gttttacac atcaaaatc 
      attaataat gctaaaatg gataaaaca caaatgcct 
      gaacaaacc aatcatgct ttattccaa ctaaccggt 
      gccttacga aacatagtt tcagaagaa actatttat 
      gaaacattc atttcgtgt ggagctatt caacagtta 
      tgtcagacc atggagctt ttctcggcg gatttggat 
      attattagt aacatatca cgtattttc agtacaata 
      tcaaccaac gattgttgt tgcgatagc ttagttgaa 
      tatagagat atttataaa gtgattatt caattattc 
      gataaatat cccggaaat gaggaaatt attgtacgt 
      ctggcgtac actttagga aacattgtc gccaaaatt 
      gataacact cgagtcaaa ttctatcac gagaaaaat 
      tcgctaaac agtctcctg aacttgtgg aagatttat 
      ttagagcgg acgctgaaa atctgctcc ctcaaaacc 
      gacaacgaa ttaatcgaa aatggaaat ccggaagac 
      gtcatgatt aaagtgatt cgagtcatt gcaaacgtt 
      gtcataaac ccagaaatc ggaaaagcc gtaaatgag 
      tcctttggc tgcctcctc atcgacgaa ttcctcaaa 
      gtcctaatc agcaatccc ttcaaaaag aacgaagaa 
      ctcgtcatg tccatcttg agtacttta aacaaccta 
      tcgtactat tacacctcc gacacagaa acggacatt 
      ttccacatc aagcaagtg gaaatcgtt gagggaatc 
      accgaatat gcaaaatca cgcaacaaa gaatgcgta 
      attgaatcg atgagaatc atgggcaat ttatcacgc 
      tcgaaaatt acgagagat tacattgcg gaatcggaa 
      gtttttaaa attcttcta aatattcta gacaaagcc 
      gatttgact ctcctcaaa acgacgatc ggagtattt 
      gttaatttg atggcagac aataagtca cgaaatttg 
      ttcaagaat cagggagga gttgcgaaa ttgatcacg 
      attttgaaa aactacgga caaaatgat tggttattg 
      gggatgttg gtgtgtcaa gtgatttgg aattattgt 
      attgatacg atggattta tacgaactt atccctgat 
      gatgagatt caacaattg ctcgtgatt ttggccgat 
      tatctcgat gaagaaaaa ttgttcgga atcacggaa 
      gggtccgaa gatactgaa gtatacgta acccaggag 
      tacttaatt tgggaggag tttgccagc gtcgcgact 
      aatttactg gaaaaaatc gaatacttt ttggacact 
      tttgaccaa attcatatt gaaaacgaa gccacactt 
      actaaggat tcgagtaca aatctcagt ttcgcagct 
      tggtaa
    mRNA sequence
    • >TC034612 RA
      gatttccaa tttcgttca agattctag aaaatgctg 
      aaacagtgg aaaagtcgc agtttgcag ccaggaact 
      caacgataa acaagcccg ttcttaatc caacaatgt 
      aacaaaata atggaacat agaagtgac gaaattaaa 
      ttaggggga ataagtaaa gtgtctttg ccattttat 
      gaaccccca ccaagaaaa accagtgcg gaaattatc 
      aacgaagcg cgccttgct ataagagaa tcacaaatg 
      gctgagagc tcctttatt tcaactcct atcaaacct 
      ctgcaaacg caaaggcct ttcacaccg agaaacaag 
      gaaaggcta ctgtttggc aaaaaaacc aaagcgaat 
      cggccgcca agttccttc agtctccga tacctccaa 
      aatgaggcg gaaatacca cccaacgtc cccgaatca 
      aacatgatc ctaagccct cacatgtta tcatcagcc 
      tcaaaaacc aacactcgg ccaattttc accaaacaa 
      cgttcaaac tcactatcc gaagtcaac gacaacaaa 
      atcttcaat atcggattc aaagaaatc tcactcaat 
      aaagtcaaa ctgccctca ctggaccac atgaaaccg 
      ttgcaaaaa cgcaaaatc ttcaaaaac acatctctc 
      gacaatctg cccgaagaa caagaatca aattctgcc 
      gactcgaaa accgaacgc aaagctttc agttctcca 
      caagaacgc acagatttc aacgaagat gattccccg 
      tttggacgc tcgtccagc aaacagaaa aatctctcg 
      caatgtctt ctcctaggt ccacaaaac ttgaacaaa 
      atcttcgat aatagacac attattgag aattctgag 
      acgctgttt gtggggaca gaaaataag gaacgaaac 
      atcgacgat gttattgac gatttaaat aatgagagc 
      aatcgaatg gacaacgaa gattttgtc attgaatta 
      ttagaagaa ctttatgga aaaatggaa aaagcgaat 
      ttgctttcg accaaagtc agttcgaaa atgaagatt 
      cacgtgctg aaagctttg tacaagttt gtggaatcg 
      caaaatgag cggcttttg ttgaacatt gccagggtt 
      attttgggg ctgaaggta actggcaac aatctcagt 
      ggagtttgt aaattgatt ttcaaagta tccaagaat 
      gacaaaaac gactactta ttcttccag aaaaatatt 
      ttagaatta tttttggac gctttgggc cgttcaagc 
      cctttagac gatgccgag gcttgcgtt tacggttac 
      ggttccgtc aaattttta acaatgaat gcgaaatta 
      ctcgataaa attttatca ctagggata ttacagttg 
      atggtttta cacatcaaa atcattaat aatgctaaa 
      atggataaa acacaaatg cctgaacaa accaatcat 
      gctttattc caactaacc ggtgcctta cgaaacata 
      gtttcagaa gaaactatt tatgaaaca ttcatttcg 
      tgtggagct attcaacag ttatgtcag accatggag 
      cttttctcg gcggatttg gatattatt agtaacata 
      tcacgtatt ttcagtaca atatcaacc aacgattgt 
      tgttgcgat agcttagtt gaatataga gatatttat 
      aaagtgatt attcaatta ttcgataaa tatcccgga 
      aatgaggaa attattgta cgtctggcg tacacttta 
      ggaaacatt gtcgccaaa attgataac actcgagtc 
      aaattctat cacgagaaa aattcgcta aacagtctc 
      ctgaacttg tggaagatt tatttagag cggacgctg 
      aaaatctgc tccctcaaa accgacaac gaattaatc 
      gaaaatgga aatccggaa gacgtcatg attaaagtg 
      attcgagtc attgcaaac gttgtcata aacccagaa 
      atcggaaaa gccgtaaat gagtccttt ggctgcctc 
      ctcatcgac gaattcctc aaagtccta atcagcaat 
      cccttcaaa aagaacgaa gaactcgtc atgtccatc 
      ttgagtact ttaaacaac ctatcgtac tattacacc 
      tccgacaca gaaacggac attttccac atcaagcaa 
      gtggaaatc gttgaggga atcaccgaa tatgcaaaa 
      tcacgcaac aaagaatgc gtaattgaa tcgatgaga 
      atcatgggc aatttatca cgctcgaaa attacgaga 
      gattacatt gcggaatcg gaagttttt aaaattctt 
      ctaaatatt ctagacaaa gccgatttg actctcctc 
      aaaacgacg atcggagta tttgttaat ttgatggca 
      gacaataag tcacgaaat ttgttcaag aatcaggga 
      ggagttgcg aaattgatc acgattttg aaaaactac 
      ggacaaaat gattggtta ttggggatg ttggtgtgt 
      caagtgatt tggaattat tgtattgat acgatggat 
      ttatacgaa cttatccct gatgatgag attcaacaa 
      ttgctcgtg attttggcc gattatctc gatgaagaa 
      aaattgttc ggaatcacg gaagggtcc gaagatact 
      gaagtatac gtaacccag gagtactta atttgggag 
      gagtttgcc agcgtcgcg actaattta ctggaaaaa 
      atcgaatac tttttggac acttttgac caaattcat 
      attgaaaac gaagccaca cttactaag gattcgagt 
      acaaatctc agtttcgca gcttggtaa taaactagg 
      gtagaagga caagttata tttata
    • >TC034612 RB
      gatttccaa tttcgttca agattctag aaaatgctg 
      aaacagtgg aaaagtcgc agtttgcag ccaggaact 
      caacgataa acaagcccg ttcttaatc caacaatgt 
      aacaaaata atggaacat agaagtgac gaaattaaa 
      ttaggggga ataagtaaa gtgtctttg ccattttat 
      gaaccccca ccaagaaaa accagtgcg gaaattatc 
      aacgaagcg cgccttgct ataagagaa tcacaaatg 
      gctgagagc tcctttatt tcaactcct atcaaacct 
      ctgcaaacg caaaggcct ttcacaccg agaaacaag 
      gaaaggcta ctgtttggc aaaaaaacc aaagcgaat 
      cggccgcca agttccttc agtctccga tacctccaa 
      aatgaggcg gaaatacca cccaacgtc cccgaatca 
      aacatgatc ctaagccct cacatgtta tcatcagcc 
      tcaaaaacc aacactcgg ccaattttc accaaacaa 
      cgttcaaac tcactatcc gaagtcaac gacaacaaa 
      atcttcaat atcggattc aaagaaatc tcactcaat 
      aaagtcaaa ctgccctca ctggaccac atgaaaccg 
      ttgcaaaaa cgcaaaatc ttcaaaaac acatctctc 
      gacaatctg cccgaagaa caagaatca aattctgcc 
      gactcgaaa accgaacgc aaagctttc agttctcca 
      caagaacgc acagatttc aacgaagat gattccccg 
      tttggacgc tcgtccagc aaacagaaa aatctctcg 
      caatgtctt ctcctaggt ccacaaaac ttgaacaaa 
      atcttcgat aatagacac attattgag aattctgag 
      acgctgttt gtggggaca ggttacttg aataggggg 
      caaaatcca caccgtgga aataaagta caaaattca 
      attttagaa aataaggaa cgaaacatc gacgatgtt 
      attgacgat ttaaataat gagagcaat cgaatggac 
      aacgaagat tttgtcatt gaattatta gaagaactt 
      tatggaaaa atggaaaaa gcgaatttg ctttcgacc 
      aaagtcagt tcgaaaatg aagattcac gtgctgaaa 
      gctttgtac aagtttgtg gaatcgcaa aatgagcgg 
      cttttgttg aacattgcc agggttatt ttggggctg 
      aaggtaact ggcaacaat ctcagtgga gtttgtaaa 
      ttgattttc aaagtatcc aagaatgac aaaaacgac 
      tacttattc ttccagaaa aatatttta gaattattt 
      ttggacgct ttgggccgt tcaagccct ttagacgat 
      gccgaggct tgcgtttac ggttacggt tccgtcaaa 
      tttttaaca atgaatgcg aaattactc gataaaatt 
      ttatcacta gggatatta cagttgatg gttttacac 
      atcaaaatc attaataat gctaaaatg gataaaaca 
      caaatgcct gaacaaacc aatcatgct ttattccaa 
      ctaaccggt gccttacga aacatagtt tcagaagaa 
      actatttat gaaacattc atttcgtgt ggagctatt 
      caacagtta tgtcagacc atggagctt ttctcggcg 
      gatttggat attattagt aacatatca cgtattttc 
      agtacaata tcaaccaac gattgttgt tgcgatagc 
      ttagttgaa tatagagat atttataaa gtgattatt 
      caattattc gataaatat cccggaaat gaggaaatt 
      attgtacgt ctggcgtac actttagga aacattgtc 
      gccaaaatt gataacact cgagtcaaa ttctatcac 
      gagaaaaat tcgctaaac agtctcctg aacttgtgg 
      aagatttat ttagagcgg acgctgaaa atctgctcc 
      ctcaaaacc gacaacgaa ttaatcgaa aatggaaat 
      ccggaagac gtcatgatt aaagtgatt cgagtcatt 
      gcaaacgtt gtcataaac ccagaaatc ggaaaagcc 
      gtaaatgag tcctttggc tgcctcctc atcgacgaa 
      ttcctcaaa gtcctaatc agcaatccc ttcaaaaag 
      aacgaagaa ctcgtcatg tccatcttg agtacttta 
      aacaaccta tcgtactat tacacctcc gacacagaa 
      acggacatt ttccacatc aagcaagtg gaaatcgtt 
      gagggaatc accgaatat gcaaaatca cgcaacaaa 
      gaatgcgta attgaatcg atgagaatc atgggcaat 
      ttatcacgc tcgaaaatt acgagagat tacattgcg 
      gaatcggaa gtttttaaa attcttcta aatattcta 
      gacaaagcc gatttgact ctcctcaaa acgacgatc 
      ggagtattt gttaatttg atggcagac aataagtca 
      cgaaatttg ttcaagaat cagggagga gttgcgaaa 
      ttgatcacg attttgaaa aactacgga caaaatgat 
      tggttattg gggatgttg gtgtgtcaa gtgatttgg 
      aattattgt attgatacg atggattta tacgaactt 
      atccctgat gatgagatt caacaattg ctcgtgatt 
      ttggccgat tatctcgat gaagaaaaa ttgttcgga 
      atcacggaa gggtccgaa gatactgaa gtatacgta 
      acccaggag tacttaatt tgggaggag tttgccagc 
      gtcgcgact aatttactg gaaaaaatc gaatacttt 
      ttggacact tttgaccaa attcatatt gaaaacgaa 
      gccacactt actaaggat tcgagtaca aatctcagt 
      ttcgcagct tggtaataa actagggta gaaggacaa 
      gttatattt ata
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