Status
Please wait ...
Gene information
General information
TC012899 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18702720..18727354
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012899
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05353

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012899 RA
      MLVKGRPCV ELLRLWEGY SNVDSDVKT KRTMEEELS 
      LFLRKLDEL EKIWAVLPG TDKLRRKAR KMRMSVKNE 
      GSLNAGGKF EWVDSVLIK CLQEGSWLL VDNVNLCSA 
      AVLDRLNAL LEPNGVLTV GERGVDEKG QMVEIVPHP 
      DFRLFLTMD PKNGEISRA MRNRGVEIY MLNEKENEH 
      RNTLDIKSL ISREGLKNI NHIESLLHL HDFTSELIL 
      GEKPNVNEL LRASALISQ QIQHGSNQL DAFHNTIME 
      IYYKTRSNM EFTCNDFDT VIKTEIGKV LHQATAQQF 
      YDAVTLKTD KLHVGTDLE KIKQQATAL QNNPVDKYF 
      LIHFYSVTS KQDLELRSR YVNCLIGDN HSVAISQTM 
      HNFIESFPV EFKDFPIDG RWIPETNYR GKCCFASNR 
      LILSLHFIA NRTMEQLCG KKPGQVTLH DYMTQRQEN 
      TISDQFDDV LVDEFLHLI ESFDSSLLK VLTDKIELS 
      DLDVIKLLE LLTWRFIFN RCTRIDVRK ITPNQLTSL 
      KVHYRWFLK YSVNSVELL LNKQSRELE DVLAKINKS 
      LEKQFSPLH KFSQTFQKR SKPPPRVAS LEIDLATCY 
      QQVLRQFDA LKHANLRNK IISLMLDRG TSLDVKISE 
      LKMLDFGEN AANLSESEV KLLPVVEFL VKLQLRNHF 
      EGQDFRSCL SVPIDLVTA LCLYRESKD GRLCHSLLR 
      SFFAYLASS DCGFQPVLS HCLLDETAK FGNFRDLMK 
      QHRQLGLIL WRNLQQLHE NAYDYRANE CEFVCQSFE 
      GFIAMISES LGLKTGDFH QLVAICLRE LETVCTDEL 
      SESFLGHLR NCAKTYRSL NECEDFHLS LLYVHDMHV 
      ELSYAKAIL NSRLPLIDP SVKKALKKK YSVETIRSF 
      EDLRDSFVQ MNEVYSGNV KTLHSHYKP VVDMIQTLN 
      IRNEQLGSY VAVRPREVL YEDVVKVVN HAFSTILSC 
      RYVDGIIQN VDSVVRDLI RGEWTPDSE RILKQYESS 
      LSSYSNLIN EWSKYAPSY PDVIEPLLS NLSEFLYGL 
      KSNLALVRK LVLRHECAK FGFDMQDEL GALVAIPSL 
      DRQHIRYES LIESYSKPQ VHKFIDYAL GSEENANVK 
      KERLRLLKC GIQETFNNC ILDVNSTGI LNFSHFENF 
      LKAFVSAWN EQEKNRELA EAEASALYK TRTKCDDKP 
      EAEQIEDEL KELFPNYHD QDFADIMRK VDEIESVEK 
      KSQPDVISH EDVKFVVDL HVSLVQNFT KTEWLNPEK 
      DKTVVVDFL NPLIEKHKL FKQICGKVL PALDYRLDK 
      DLVESMSVL LCFVQNYGD TNYLVEPPS PVKISQSKR 
      FDFYKDSNI EEVKSSFHI LEELRIRTR SLLSEWPDH 
      PTLKSIMTI IERVYSFDL ASPLLRFLT AFEILLSKC 
      NEWEEVAHS GVSLSENAQ TITHQVIAW RKMELNVWK 
      DLLNSTFTR MNGPTYKWW LYLYNIMEQ FEEKYTEIE 
      LIEALQNYV TKSNLAEFQ TRLNLLHAF HCHAVHRKR 
      TDKSDRFVS ILWNVYNYF LQFAQIVNN KIKDLRAPI 
      EKKLKDYVK IVRWKDVNY WAIKETVDK SHKTLHKYV 
      REFQVSLVI
    CDS sequence
    • >TC012899 RA
      atgctcgta aaaggtcga ccctgtgtc gaattgctc 
      cgtctttgg gagggatac tcgaacgtg gatagtgac 
      gtgaagact aagcgcacc atggaggaa gaactgagc 
      ctatttctg cgaaaactt gacgaattg gagaagatt 
      tgggcggtt ttgcctgga acagataag ctacgtagg 
      aaagctcga aaaatgagg atgagtgtg aaaaatgag 
      ggcagtttg aatgccggt ggtaaattc gaatgggtc 
      gactcggtt ttgataaaa tgtttacaa gaaggttcg 
      tggttgctc gtggataac gttaacttg tgtagtgcg 
      gccgttttg gatcgactt aatgcgctt ttagaaccg 
      aacggtgtt ttaacagtg ggcgagaga ggggtggat 
      gagaaggga caaatggtt gaaattgtt cctcatccc 
      gatttcagg ctgttttta acgatggat cccaagaat 
      ggagaaata tcgagggcc atgaggaac cgaggcgtt 
      gaaatttac atgctcaat gagaaagaa aacgaacac 
      cgcaacact ctcgatata aaaagccta ataagccgc 
      gagggactg aaaaatatt aaccacatc gaatccctg 
      ttacatttg catgatttt accagcgag ttgattttg 
      ggcgaaaaa ccaaatgtg aacgaatta ttacgcgca 
      tcggctcta atttcccaa caaatccaa cacggctcg 
      aaccaactc gacgcattc cacaacacc atcatggaa 
      atttattac aaaacgcgc tccaacatg gagttcact 
      tgcaatgat ttcgatacg gttattaaa accgaaatc 
      ggaaaggtg ctacaccag gcgactgca caacaattc 
      tacgacgct gtcacgcta aaaacggat aaattgcac 
      gtcgggacg gatttggag aaaattaaa cagcaagca 
      acggcgcta caaaataac ccagttgat aaatatttc 
      ttgattcac ttctacagt gttaccagt aaacaggat 
      ttggagctg cgctctcgc tacgttaat tgtttaatt 
      ggtgataat cattcggtc gcgatttct caaacaatg 
      cacaacttc attgagagt ttcccagtt gagtttaag 
      gactttccg attgatggg cgttggatc cctgaaact 
      aattacagg gggaaatgt tgttttgcc agcaacagg 
      ctaatcctc agtctgcat tttattgca aaccgtacg 
      atggaacaa ttatgcgga aaaaagcca ggtcaggtc 
      acactacat gactacatg acccaacga caagaaaat 
      acaatcagc gaccagttc gatgatgtt ttagtcgat 
      gaatttttg cacttaatt gaatcattc gatagttcc 
      ttgctgaaa gtcctcacg gacaagatt gaactaagc 
      gacctggac gtaatcaaa cttctggaa ttactgact 
      tggcggttt attttcaat cgttgcacg cggatagac 
      gtgcgaaaa atcacccca aatcagctg acaagtctc 
      aaagtgcac tatcgatgg tttttgaaa tattcggta 
      aacagcgtg gaattactt ttgaacaaa caatcgcgc 
      gaactggag gatgttttg gcaaagatt aataaaagt 
      ctggaaaaa caattctcc ccacttcat aaattcagt 
      caaaccttc cagaaacgt agtaaacca ccaccgaga 
      gtcgccagt ttggagata gacttagca acgtgttac 
      caacaagtt ttgaggcaa tttgacgct ttgaaacat 
      gcaaatttg aggaacaaa atcatcagt ttgatgctt 
      gatcgcgga acgagtctg gacgtgaaa atcagcgaa 
      cttaaaatg cttgatttt ggcgaaaac gccgctaat 
      ttaagcgag tctgaggtg aagttgctg ccagtggtt 
      gagttcctg gtcaagttg caattgagg aatcacttc 
      gaggggcaa gatttccgg tcgtgtctg agcgttccg 
      attgattta gtgaccgct ctttgtctg tacagagag 
      agcaaagac ggccgactt tgtcacagt ttgttgcgt 
      tcgttcttt gcgtatttg gcgagctcg gactgtggt 
      tttcagccg gttttgagt cactgtttg ttggacgaa 
      acggccaag tttgggaat ttccgcgat ttgatgaaa 
      cagcatcga caattgggt ttgattttg tggcggaat 
      ttgcagcaa ttgcacgaa aacgcctat gattatcgc 
      gcaaacgag tgtgagttt gtttgccaa agcttcgaa 
      gggtttatt gcgatgatt tcggagagt ttgggtttg 
      aaaaccggt gatttccac caattagtt gcgatttgt 
      ttgagggaa ttggagaca gtttgcact gatgagtta 
      agtgagtcg tttctgggc catttgagg aattgtgcg 
      aagacgtac cgaagtttg aatgagtgt gaggacttc 
      cacttgagt cttttgtac gtccatgac atgcatgtg 
      gagttgagc tatgctaaa gccattctc aactcgcga 
      ttgcccttg atagaccct tcggtgaag aaggcttta 
      aaaaagaaa tacagtgtt gagactatt cgtagtttc 
      gaggatttg agagatagt tttgttcaa atgaatgaa 
      gtttatagc gggaatgtc aaaactttg cattcgcat 
      tacaaaccc gtcgtcgac atgattcaa actttaaat 
      atcaggaac gaacaactg gggagttat gtcgctgtg 
      aggcccaga gaagtgctc tacgaagac gtcgtcaag 
      gtggtaaat cacgccttt tccacgatt ttatcctgt 
      cgatacgtc gacgggatt atccagaat gtggattcg 
      gtggtcagg gatttaatc agaggcgag tggaccccg 
      gactcggag cgaatttta aaacagtac gagtcctca 
      ctttcaagt tattctaat ctgatcaac gagtggtcg 
      aaatatgcg ccctcttat cccgacgtt atcgagcct 
      cttttgagt aacttgagc gagtttttg tacggtctc 
      aaatcgaat ttggctctg gtacgaaag ctcgtcttg 
      agacacgaa tgtgcaaag ttcgggttt gacatgcaa 
      gacgaactc ggagctttg gttgcgatt ccgagtctt 
      gaccgccaa catattcgc tatgaatcg ctaattgag 
      agctactcc aagccgcaa gttcataag ttcatcgat 
      tatgcgctc gggagtgag gaaaacgca aatgtgaag 
      aaggagcgg ttgagactg ctgaagtgc ggcatccaa 
      gaaacattt aataattgt atcctggac gtaaactcg 
      acgggaata ttaaatttc tcacatttc gaaaatttc 
      cttaaagcc ttcgtgagc gcgtggaac gagcaggag 
      aaaaatcgc gagcttgca gaggcagaa gccagcgct 
      ttgtacaaa accaggacg aaatgtgac gataagccc 
      gaagccgaa caaatcgaa gacgaactc aaagaatta 
      ttcccaaat taccacgac caagatttc gctgatata 
      atgcgaaaa gttgacgaa atcgaatcg gtcgagaaa 
      aaatcacaa cccgatgtt atttcgcac gaggatgtt 
      aaattcgtt gttgatttg catgtctcg cttgtgcag 
      aattttacc aaaactgag tggttgaat ccggagaag 
      gataaaact gtagttgtg gatttcctg aatccgttg 
      attgaaaaa cacaaattg tttaaacaa atttgtggg 
      aaggtttta ccggcgttg gactaccgg ctggataag 
      gatttagtg gaaagtatg agcgtcttg ctctgtttc 
      gtgcagaat tacggagat acaaattat ttagtcgaa 
      cctccatct cccgtaaaa atctcccag tcaaaacgt 
      ttcgacttt tacaaagac tcaaatatc gaagaagtg 
      aaatcttcc ttccacatt ttggaggag ctccgcatt 
      cggaccagg tcccttctg tccgaatgg cccgaccac 
      ccaacccta aaatcgatt atgactatt attgagcgc 
      gtctacagt ttcgaccta gccagtccg ttactgcgc 
      tttttgaca gctttcgaa attctgtta agcaaatgc 
      aacgaatgg gaagaagtg gcccattct ggcgtcagt 
      ctgagcgaa aacgcccaa accatcaca caccaagta 
      atcgcgtgg cgaaaaatg gaattaaac gtttggaag 
      gatttattg aacagtaca tttacgcga atgaacggc 
      cctacatac aaatggtgg ctgtacttg tacaacata 
      atggagcaa ttcgaggaa aaatacacc gagattgag 
      ctaatcgag gcacttcag aattatgtc acgaagtca 
      aatttggcc gaattccag accagattg aatctatta 
      catgcgttc cactgccat gctgtacat cgaaagcgc 
      acggacaaa tcggacaga tttgtcagc attttgtgg 
      aatgtttac aattacttc ctgcaattc gcgcaaatt 
      gtgaacaac aaaatcaag gatttgagg gcacccatc 
      gagaagaag ctcaaagat tacgtcaag atcgtgaga 
      tggaaggat gttaattat tgggccatc aaagaaacc 
      gtcgataag tcgcataaa acgctccat aaatacgtc 
      agagaattt caggtaagt ctcgttatt taa
    mRNA sequence
    • >TC012899 RA
      tcagactga ttactccag gcatttaga ggagatcgt 
      gagggaaac ggaacaact tttgttaaa aatcgtgcg 
      gacaatgct cgtaaaagg tcgaccctg tgtcgaatt 
      gctccgtct ttgggaggg atactcgaa cgtggatag 
      tgacgtgaa gactaagcg caccatgga ggaagaact 
      gagcctatt tctgcgaaa acttgacga attggagaa 
      gatttgggc ggttttgcc tggaacaga taagctacg 
      taggaaagc tcgaaaaat gaggatgag tgtgaaaaa 
      tgagggcag tttgaatgc cggtggtaa attcgaatg 
      ggtcgactc ggttttgat aaaatgttt acaagaagg 
      ttcgtggtt gctcgtgga taacgttaa cttgtgtag 
      tgcggccgt tttggatcg acttaatgc gcttttaga 
      accgaacgg tgttttaac agtgggcga gagaggggt 
      ggatgagaa gggacaaat ggttgaaat tgttcctca 
      tcccgattt caggctgtt tttaacgat ggatcccaa 
      gaatggaga aatatcgag ggccatgag gaaccgagg 
      cgttgaaat ttacatgct caatgagaa agaaaacga 
      acaccgcaa cactctcga tataaaaag cctaataag 
      ccgcgaggg actgaaaaa tattaacca catcgaatc 
      cctgttaca tttgcatga ttttaccag cgagttgat 
      tttgggcga aaaaccaaa tgtgaacga attattacg 
      cgcatcggc tctaatttc ccaacaaat ccaacacgg 
      ctcgaacca actcgacgc attccacaa caccatcat 
      ggaaattta ttacaaaac gcgctccaa catggagtt 
      cacttgcaa tgatttcga tacggttat taaaaccga 
      aatcggaaa ggtgctaca ccaggcgac tgcacaaca 
      attctacga cgctgtcac gctaaaaac ggataaatt 
      gcacgtcgg gacggattt ggagaaaat taaacagca 
      agcaacggc gctacaaaa taacccagt tgataaata 
      tttcttgat tcacttcta cagtgttac cagtaaaca 
      ggatttgga gctgcgctc tcgctacgt taattgttt 
      aattggtga taatcattc ggtcgcgat ttctcaaac 
      aatgcacaa cttcattga gagtttccc agttgagtt 
      taaggactt tccgattga tgggcgttg gatccctga 
      aactaatta cagggggaa atgttgttt tgccagcaa 
      caggctaat cctcagtct gcattttat tgcaaaccg 
      tacgatgga acaattatg cggaaaaaa gccaggtca 
      ggtcacact acatgacta catgaccca acgacaaga 
      aaatacaat cagcgacca gttcgatga tgttttagt 
      cgatgaatt tttgcactt aattgaatc attcgatag 
      ttccttgct gaaagtcct cacggacaa gattgaact 
      aagcgacct ggacgtaat caaacttct ggaattact 
      gacttggcg gtttatttt caatcgttg cacgcggat 
      agacgtgcg aaaaatcac cccaaatca gctgacaag 
      tctcaaagt gcactatcg atggttttt gaaatattc 
      ggtaaacag cgtggaatt acttttgaa caaacaatc 
      gcgcgaact ggaggatgt tttggcaaa gattaataa 
      aagtctgga aaaacaatt ctccccact tcataaatt 
      cagtcaaac cttccagaa acgtagtaa accaccacc 
      gagagtcgc cagtttgga gatagactt agcaacgtg 
      ttaccaaca agttttgag gcaatttga cgctttgaa 
      acatgcaaa tttgaggaa caaaatcat cagtttgat 
      gcttgatcg cggaacgag tctggacgt gaaaatcag 
      cgaacttaa aatgcttga ttttggcga aaacgccgc 
      taatttaag cgagtctga ggtgaagtt gctgccagt 
      ggttgagtt cctggtcaa gttgcaatt gaggaatca 
      cttcgaggg gcaagattt ccggtcgtg tctgagcgt 
      tccgattga tttagtgac cgctctttg tctgtacag 
      agagagcaa agacggccg actttgtca cagtttgtt 
      gcgttcgtt ctttgcgta tttggcgag ctcggactg 
      tggttttca gccggtttt gagtcactg tttgttgga 
      cgaaacggc caagtttgg gaatttccg cgatttgat 
      gaaacagca tcgacaatt gggtttgat tttgtggcg 
      gaatttgca gcaattgca cgaaaacgc ctatgatta 
      tcgcgcaaa cgagtgtga gtttgtttg ccaaagctt 
      cgaagggtt tattgcgat gatttcgga gagtttggg 
      tttgaaaac cggtgattt ccaccaatt agttgcgat 
      ttgtttgag ggaattgga gacagtttg cactgatga 
      gttaagtga gtcgtttct gggccattt gaggaattg 
      tgcgaagac gtaccgaag tttgaatga gtgtgagga 
      cttccactt gagtctttt gtacgtcca tgacatgca 
      tgtggagtt gagctatgc taaagccat tctcaactc 
      gcgattgcc cttgataga cccttcggt gaagaaggc 
      tttaaaaaa gaaatacag tgttgagac tattcgtag 
      tttcgagga tttgagaga tagttttgt tcaaatgaa 
      tgaagttta tagcgggaa tgtcaaaac tttgcattc 
      gcattacaa acccgtcgt cgacatgat tcaaacttt 
      aaatatcag gaacgaaca actggggag ttatgtcgc 
      tgtgaggcc cagagaagt gctctacga agacgtcgt 
      caaggtggt aaatcacgc cttttccac gattttatc 
      ctgtcgata cgtcgacgg gattatcca gaatgtgga 
      ttcggtggt cagggattt aatcagagg cgagtggac 
      cccggactc ggagcgaat tttaaaaca gtacgagtc 
      ctcactttc aagttattc taatctgat caacgagtg 
      gtcgaaata tgcgccctc ttatcccga cgttatcga 
      gcctctttt gagtaactt gagcgagtt tttgtacgg 
      tctcaaatc gaatttggc tctggtacg aaagctcgt 
      cttgagaca cgaatgtgc aaagttcgg gtttgacat 
      gcaagacga actcggagc tttggttgc gattccgag 
      tcttgaccg ccaacatat tcgctatga atcgctaat 
      tgagagcta ctccaagcc gcaagttca taagttcat 
      cgattatgc gctcgggag tgaggaaaa cgcaaatgt 
      gaagaagga gcggttgag actgctgaa gtgcggcat 
      ccaagaaac atttaataa ttgtatcct ggacgtaaa 
      ctcgacggg aatattaaa tttctcaca tttcgaaaa 
      tttccttaa agccttcgt gagcgcgtg gaacgagca 
      ggagaaaaa tcgcgagct tgcagaggc agaagccag 
      cgctttgta caaaaccag gacgaaatg tgacgataa 
      gcccgaagc cgaacaaat cgaagacga actcaaaga 
      attattccc aaattacca cgaccaaga tttcgctga 
      tataatgcg aaaagttga cgaaatcga atcggtcga 
      gaaaaaatc acaacccga tgttatttc gcacgagga 
      tgttaaatt cgttgttga tttgcatgt ctcgcttgt 
      gcagaattt taccaaaac tgagtggtt gaatccgga 
      gaaggataa aactgtagt tgtggattt cctgaatcc 
      gttgattga aaaacacaa attgtttaa acaaatttg 
      tgggaaggt tttaccggc gttggacta ccggctgga 
      taaggattt agtggaaag tatgagcgt cttgctctg 
      tttcgtgca gaattacgg agatacaaa ttatttagt 
      cgaacctcc atctcccgt aaaaatctc ccagtcaaa 
      acgtttcga cttttacaa agactcaaa tatcgaaga 
      agtgaaatc ttccttcca cattttgga ggagctccg 
      cattcggac caggtccct tctgtccga atggcccga 
      ccacccaac cctaaaatc gattatgac tattattga 
      gcgcgtcta cagtttcga cctagccag tccgttact 
      gcgcttttt gacagcttt cgaaattct gttaagcaa 
      atgcaacga atgggaaga agtggccca ttctggcgt 
      cagtctgag cgaaaacgc ccaaaccat cacacacca 
      agtaatcgc gtggcgaaa aatggaatt aaacgtttg 
      gaaggattt attgaacag tacatttac gcgaatgaa 
      cggccctac atacaaatg gtggctgta cttgtacaa 
      cataatgga gcaattcga ggaaaaata caccgagat 
      tgagctaat cgaggcact tcagaatta tgtcacgaa 
      gtcaaattt ggccgaatt ccagaccag attgaatct 
      attacatgc gttccactg ccatgctgt acatcgaaa 
      gcgcacgga caaatcgga cagatttgt cagcatttt 
      gtggaatgt ttacaatta cttcctgca attcgcgca 
      aattgtgaa caacaaaat caaggattt gagggcacc 
      catcgagaa gaagctcaa agattacgt caagatcgt 
      gagatggaa ggatgttaa ttattgggc catcaaaga 
      aaccgtcga taagtcgca taaaacgct ccataaata 
      cgtcagaga atttcaggt aagtctcgt tatttaatt 
      ggatttttt cactcgagt tttgctgca aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY