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Gene information
General information
TC034613 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18896969..18940317
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034613
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05352

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: 3 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1
    egg chamber fused - Number of animals showing the phenotype: 5

    egg chamber segmentation/division not present - Number of animals showing the phenotype: 5
    tropharium slightlystructure irregular - Number of animals showing the phenotype: 3

    Comment: The tropharia look rougher. This was true for sthe stronger phenotypes.
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    almost none

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034613 RA
      MTRTVFFYK WCATLDPAA DNEKNTPLR MQIIHPGTA 
      QEGINSNEL LAVRPPFIP FQAVADHPT LFAQTATEG 
      HTFSKTGLG NVQVIAQPT AASQLAVGQ HIQQDPNDP 
      NKWQVIPNV TTTQQTTAP LTPGVVVTA NPPEINNST 
      PVGENGQDG LQKQRVRRV ACTCPNCQE GERHADRKK 
      QHICHIPGC NKVYGKTSH LRAHLRWHT GERPFVCSW 
      LFCGKRFTR SDELQRHRR THTGEKRFQ CNECSKKFM 
      RSDHLSKHL KTHQKQRIT RINGDDIDD VHEEKDEWD 
      DAQSDEQAK PMWITDNKN NIKYEITQV NYAQEAATS 
      TQSASSDSS SEEKMMITI STSEADELI IADPLDS
    • >TC034613 RB
      MFERTTSAT PTRPKSCVG RRTFCRFLS SPHTRTTSR 
      SASTPTRRS HFLHREGKS VTKKLEKPL LASCVRRPA 
      IWKSARKLE RDTVDEAAP RTSPQTQER RHSYLRRRR 
      RTEISPPAA APFSIKCVC FLGADEMRE ESRAGRTHR 
      GLTQGAART MLDLTDSNL SQKPEMTRT VFFYKWCAT 
      LDPAADNEK NTPLRMQII HPGTAQEGI NSNELLAVR 
      PPFIPFQAV ADHPTLFAQ TATEGHTFS KTGLGNVQV 
      IAQPTAASQ LAVGQHIQQ DPNDPNKWQ VIPNVTTTQ 
      QTTAPLTPG VVVTANPPE INNSTPVGE NGQDGLQKQ 
      RVRRVACTC PNCQEGERH ADRKKQHIC HIPGCNKVY 
      GKTSHLRAH LRWHTGERP FVCSWLFCG KRFTRSDEL 
      QRHRRTHTG EKRFQCNEC SKKFMRSDH LSKHLKTHQ 
      KQRITRING DDIDDVHEE KDEWDDAQS DEQAKPMWI 
      TDNKNNIKY EITQVNYAQ EAATSTQSA SSDSSSEEK 
      MMITISTSE ADELIIADP LDS
    CDS sequence
    • >TC034613 RA
      atgacgcgc actgttttc ttttacaaa tggtgtgcc 
      acacttgac cccgccgcc gacaacgag aaaaacaca 
      ccgctccgc atgcagata attcatccg gggacggcg 
      caggaaggg attaattca aatgaattg ctcgcggtg 
      cgaccacct ttcatcccg tttcaagcg gttgccgac 
      cacccgacc ctttttgcc caaacggca acagaaggg 
      cacactttt tcgaaaacc ggtctgggt aacgtgcaa 
      gtaatcgcg caaccaaca gccgcatca caactcgcc 
      gtaggccaa cacatccag caggacccc aacgacccc 
      aacaaatgg caagtgatc cccaacgtg accacaacg 
      caacaaact acagcacca ctcactccc ggagtcgtg 
      gtgaccgcc aaccctccc gaaataaac aattcgact 
      ccagtcggg gaaaacggc caagacggt ttacaaaaa 
      cagcgtgtt cggagggtg gcttgcacg tgtcccaac 
      tgtcaagag ggcgaacga cacgccgat cgcaagaaa 
      caacacatc tgccacatc ccgggatgc aataaagtg 
      tacggaaaa acatcacat ctcagagcg caccttcgg 
      tggcatacg ggcgaaagg ccgtttgtg tgtagttgg 
      ctgttctgt gggaagcgc ttcacgcgc tcagatgag 
      ttgcaaaga cataggagg acacatact ggggagaag 
      cgcttccag tgcaacgaa tgcagcaag aagtttatg 
      aggtcggat cacttgtcc aaacatctc aagacgcac 
      caaaagcag aggattacg cggattaat ggagacgac 
      attgacgat gttcacgaa gaaaaagat gagtgggat 
      gacgcccag tcggacgag caagcaaaa cctatgtgg 
      attacagac aataaaaac aatattaaa tacgaaatc 
      actcaagtt aattacgca caggaagct gcaacgtcg 
      acacagtct gcatcgtcg gatagctcg agtgaagaa 
      aaaatgatg attacgatc agcacgtct gaagccgat 
      gaactgatc attgctgat ccgcttgat agctga
    • >TC034613 RB
      atgtttgaa cgaactacc tcagcaacc ccaacaaga 
      cccaagtcc tgcgtcgga cgtcgcaca ttttgtcgc 
      ttcttgagc tcaccacac acacgcacc acttcacga 
      agcgcctcg acgccgaca agacgctcg cattttttg 
      caccgagag ggaaaaagt gtcactaaa aagttggaa 
      aagccgctt ttggccagt tgtgttcga cgtcctgct 
      atttggaag agcgcgcgg aaactggag agagacacc 
      gttgatgaa gctgcgccg cgcacatct ccacagacg 
      caagagcga cgccactcc tacctccgt cgccgtcgc 
      cgaactgaa atctcgccc ccggcggcg gctcctttt 
      tcaataaaa tgcgtgtgt ttcttgggg gctgacgaa 
      atgagggag gaatcgcgg gccggacgc actcataga 
      gggctcaca caaggagca gcgaggaca atgctggat 
      ctaaccgac agcaattta tcacagaaa cctgaaatg 
      acgcgcact gttttcttt tacaaatgg tgtgccaca 
      cttgacccc gccgccgac aacgagaaa aacacaccg 
      ctccgcatg cagataatt catccgggg acggcgcag 
      gaagggatt aattcaaat gaattgctc gcggtgcga 
      ccacctttc atcccgttt caagcggtt gccgaccac 
      ccgaccctt tttgcccaa acggcaaca gaagggcac 
      actttttcg aaaaccggt ctgggtaac gtgcaagta 
      atcgcgcaa ccaacagcc gcatcacaa ctcgccgta 
      ggccaacac atccagcag gaccccaac gaccccaac 
      aaatggcaa gtgatcccc aacgtgacc acaacgcaa 
      caaactaca gcaccactc actcccgga gtcgtggtg 
      accgccaac cctcccgaa ataaacaat tcgactcca 
      gtcggggaa aacggccaa gacggttta caaaaacag 
      cgtgttcgg agggtggct tgcacgtgt cccaactgt 
      caagagggc gaacgacac gccgatcgc aagaaacaa 
      cacatctgc cacatcccg ggatgcaat aaagtgtac 
      ggaaaaaca tcacatctc agagcgcac cttcggtgg 
      catacgggc gaaaggccg tttgtgtgt agttggctg 
      ttctgtggg aagcgcttc acgcgctca gatgagttg 
      caaagacat aggaggaca catactggg gagaagcgc 
      ttccagtgc aacgaatgc agcaagaag tttatgagg 
      tcggatcac ttgtccaaa catctcaag acgcaccaa 
      aagcagagg attacgcgg attaatgga gacgacatt 
      gacgatgtt cacgaagaa aaagatgag tgggatgac 
      gcccagtcg gacgagcaa gcaaaacct atgtggatt 
      acagacaat aaaaacaat attaaatac gaaatcact 
      caagttaat tacgcacag gaagctgca acgtcgaca 
      cagtctgca tcgtcggat agctcgagt gaagaaaaa 
      atgatgatt acgatcagc acgtctgaa gccgatgaa 
      ctgatcatt gctgatccg cttgatagc tga
    mRNA sequence
    • >TC034613 RA
      ccacacttc atcatacag agcaattta tcacagaaa 
      cctgaaatg acgcgcact gttttcttt tacaaatgg 
      tgtgccaca cttgacccc gccgccgac aacgagaaa 
      aacacaccg ctccgcatg cagataatt catccgggg 
      acggcgcag gaagggatt aattcaaat gaattgctc 
      gcggtgcga ccacctttc atcccgttt caagcggtt 
      gccgaccac ccgaccctt tttgcccaa acggcaaca 
      gaagggcac actttttcg aaaaccggt ctgggtaac 
      gtgcaagta atcgcgcaa ccaacagcc gcatcacaa 
      ctcgccgta ggccaacac atccagcag gaccccaac 
      gaccccaac aaatggcaa gtgatcccc aacgtgacc 
      acaacgcaa caaactaca gcaccactc actcccgga 
      gtcgtggtg accgccaac cctcccgaa ataaacaat 
      tcgactcca gtcggggaa aacggccaa gacggttta 
      caaaaacag cgtgttcgg agggtggct tgcacgtgt 
      cccaactgt caagagggc gaacgacac gccgatcgc 
      aagaaacaa cacatctgc cacatcccg ggatgcaat 
      aaagtgtac ggaaaaaca tcacatctc agagcgcac 
      cttcggtgg catacgggc gaaaggccg tttgtgtgt 
      agttggctg ttctgtggg aagcgcttc acgcgctca 
      gatgagttg caaagacat aggaggaca catactggg 
      gagaagcgc ttccagtgc aacgaatgc agcaagaag 
      tttatgagg tcggatcac ttgtccaaa catctcaag 
      acgcaccaa aagcagagg attacgcgg attaatgga 
      gacgacatt gacgatgtt cacgaagaa aaagatgag 
      tgggatgac gcccagtcg gacgagcaa gcaaaacct 
      atgtggatt acagacaat aaaaacaat attaaatac 
      gaaatcact caagttaat tacgcacag gaagctgca 
      acgtcgaca cagtctgca tcgtcggat agctcgagt 
      gaagaaaaa atgatgatt acgatcagc acgtctgaa 
      gccgatgaa ctgatcatt gctgatccg cttgatagc 
      tgagcttcc atctactta atcgagtcg gaaaaactg 
      taaaaattg tttagttat gtttgaacg tttgtacaa 
      actctgtat aaagttttt accttgaat atatagttt 
      attttgta
    • >TC034613 RB
      aagcttaga aatgtttga acgaactac ctcagcaac 
      cccaacaag acccaagtc ctgcgtcgg acgtcgcac 
      attttgtcg cttcttgag ctcaccaca cacacgcac 
      cacttcacg aagcgcctc gacgccgac aagacgctc 
      gcatttttt gcaccgaga gggaaaaag tgtcactaa 
      aaagttgga aaagccgct tttggccag ttgtgttcg 
      acgtcctgc tatttggaa gagcgcgcg gaaactgga 
      gagagacac cgttgatga agctgcgcc gcgcacatc 
      tccacagac gcaagagcg acgccactc ctacctccg 
      tcgccgtcg ccgaactga aatctcgcc cccggcggc 
      ggctccttt ttcaataaa atgcgtgtg tttcttggg 
      ggctgacga aatgaggga ggaatcgcg ggccggacg 
      cactcatag agggctcac acaaggagc agcgaggac 
      aatgctgga tctaaccga cagcaattt atcacagaa 
      acctgaaat gacgcgcac tgttttctt ttacaaatg 
      gtgtgccac acttgaccc cgccgccga caacgagaa 
      aaacacacc gctccgcat gcagataat tcatccggg 
      gacggcgca ggaagggat taattcaaa tgaattgct 
      cgcggtgcg accaccttt catcccgtt tcaagcggt 
      tgccgacca cccgaccct ttttgccca aacggcaac 
      agaagggca cactttttc gaaaaccgg tctgggtaa 
      cgtgcaagt aatcgcgca accaacagc cgcatcaca 
      actcgccgt aggccaaca catccagca ggaccccaa 
      cgaccccaa caaatggca agtgatccc caacgtgac 
      cacaacgca acaaactac agcaccact cactcccgg 
      agtcgtggt gaccgccaa ccctcccga aataaacaa 
      ttcgactcc agtcgggga aaacggcca agacggttt 
      acaaaaaca gcgtgttcg gagggtggc ttgcacgtg 
      tcccaactg tcaagaggg cgaacgaca cgccgatcg 
      caagaaaca acacatctg ccacatccc gggatgcaa 
      taaagtgta cggaaaaac atcacatct cagagcgca 
      ccttcggtg gcatacggg cgaaaggcc gtttgtgtg 
      tagttggct gttctgtgg gaagcgctt cacgcgctc 
      agatgagtt gcaaagaca taggaggac acatactgg 
      ggagaagcg cttccagtg caacgaatg cagcaagaa 
      gtttatgag gtcggatca cttgtccaa acatctcaa 
      gacgcacca aaagcagag gattacgcg gattaatgg 
      agacgacat tgacgatgt tcacgaaga aaaagatga 
      gtgggatga cgcccagtc ggacgagca agcaaaacc 
      tatgtggat tacagacaa taaaaacaa tattaaata 
      cgaaatcac tcaagttaa ttacgcaca ggaagctgc 
      aacgtcgac acagtctgc atcgtcgga tagctcgag 
      tgaagaaaa aatgatgat tacgatcag cacgtctga 
      agccgatga actgatcat tgctgatcc gcttgatag 
      ctgagcttc catctactt aatcgagtc ggaaaaact 
      gtaaaaatt gtttagtta tgtttgaac gtttgtaca 
      aactctgta taaagtttt taccttgaa tatatagtt 
      tattttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY