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Gene information
General information
TC012892 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18863210..18879334
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012892
      iBeetle screen iB_05351

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
      Days post injection


      beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012892 RA
        MAFLYKLVY CVAVITVAK AIPTTKSPK 
        TYQTEETDD SYNPRPYDE PITYESFAI 
        PTNLQLPKS ESAQVPFYV PDITTALKA 
        PQYSSEPNY YQVPIPAQD LVAPSETAW 
        NPDNDPKFF YEVPASLTQ QNIPTNLYP 
        KKFNKDVHV KSKPYSSKP KQEIVLEPI 
        NEKQFLAKQ KSLTKVYDS LAKKENQKD 
        LQSQQAVQI
      CDS sequence
      • >TC012892 RA
        atggctttt ctatataaa ctggtatac tgcgttgct 
        gtgatcaca gtagcaaaa gccatacca acgacaaaa 
        agccccaag acctaccaa accgaagag accgatgac 
        agctacaac ccgcgaccg tacgacgag ccgatcacc 
        tacgagtcc ttcgccatc cccaccaac ctccaactc 
        cccaaatcc gagtcagcg caagtccct ttctacgtc 
        cctgacatc accacagcc ctaaaagcg ccccaatac 
        agctccgaa cccaactac taccaagtc cccataccg 
        gcccaagac ctcgtcgcc ccctctgaa accgcctgg 
        aacccagac aacgacccc aaattcttc tacgaagtc 
        ccggcctcc ctcacccag cagaacatc cccaccaac 
        ctctacccc aagaaattc aacaaagac gtccacgtc 
        aagagcaaa ccctactcg agcaaaccc aaacaggaa 
        attgttttg gagcccatc aacgagaaa cagttcctt 
        gccaagcag aagagtttg accaaggtt tacgacagt 
        ctggcgaag aaggagaat cagaaggat cttcagtca 
        caacaggca gttcagata tga
      mRNA sequence
      • >TC012892 RA
        gtttatatt attgttgaa gttccaagt gaaatagtg 
        tttggtgta gacaagaaa tggcttttc tatataaac 
        tggtatact gcgttgctg tgatcacag tagcaaaag 
        ccataccaa cgacaaaaa gccccaaga cctaccaaa 
        ccgaagaga ccgatgaca gctacaacc cgcgaccgt 
        acgacgagc cgatcacct acgagtcct tcgccatcc 
        ccaccaacc tccaactcc ccaaatccg agtcagcgc 
        aagtccctt tctacgtcc ctgacatca ccacagccc 
        taaaagcgc cccaataca gctccgaac ccaactact 
        accaagtcc ccataccgg cccaagacc tcgtcgccc 
        cctctgaaa ccgcctgga acccagaca acgacccca 
        aattcttct acgaagtcc cggcctccc tcacccagc 
        agaacatcc ccaccaacc tctacccca agaaattca 
        acaaagacg tccacgtca agagcaaac cctactcga 
        gcaaaccca aacaggaaa ttgttttgg agcccatca 
        acgagaaac agttccttg ccaagcaga agagtttga 
        ccaaggttt acgacagtc tggcgaaga aggagaatc 
        agaaggatc ttcagtcac aacaggcag ttcagatat 
        gaagaaaga gataaagag gggattgtg aagggtcat 
        tttggtttc ttcgataaa cgtggaaaa ttgcaaatg 
        gttcattac gacgcacat ccccatgaa ggttaccat 
        gcagatgga aatttcggc aaatattaa tttgttatt 
        agttcaatg tttcaaaac gctcaaata acactatta 
        taaatcaat gttttatat tggttttta gtattaggt 
        ttaaaggat cttgagagg tgtgtgcag ctctcttgt 
        tatctattt ttattatta tgtgaataa aagttttaa 
        taatac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY