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Gene information
General information
TC012889 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18783931..18808393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012889
      iBeetle screen iB_05349

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      ovary without detectable defects - Number of animals showing the phenotype: 2
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
      embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      cuticle remnants cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: abdomen is stronger affected than thorax and head
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      Comment: abdomen is stronger affected than thorax and head
      abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      Comment: abdomen is stronger affected than thorax and head
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012889 RA
        MIGANLRFG SSVANASLN ATKVFQCHP GGTAEAALI 
        FALGTMGMA ANIALMGVI LANRQLRRW SQGLLFHQG 
        AVDCARAAL LLPLGISIL HCQPVQKCS LVETAFLLL 
        VTVATVNML TTVLNDSPV FPEDDDSDS LSDVPLLMD 
        SPQCVLFGT FMIWFASIT INLGPTFLS GALAAQGEA 
        GHKAPSCPL IHGPLRHYI LNALWIAVN LVCVFLTLF 
        HLRKLHRDL TKANVEAVR VAGLVTTLV NVTGGGGAA 
        ANGERASGA LEEHRRMRE YLRRMERDG VQRVKMFLV 
        ITAAYVLFW GPLFFVTLV NHPIVGNPL GHEITLHIS 
        YVHAIVNPT LFLVLHRGL RRATLDLCC GWIPLWLGN 
        PNVPPPPDP PRTDISLLK PPLPVPAHP DSSMGDSDL 
        SPWTPEPVN GGIQIPQYI SPEPSSPPE SD
      CDS sequence
      • >TC012889 RA
        atgataggt gccaacctg cgtttcggc agttcggtg 
        gcgaatgca agtctcaat gccaccaag gtcttccaa 
        tgtcatcct ggagggacc gctgaagcc gcgctgata 
        ttcgcctta ggaacgatg ggaatggcc gccaatata 
        gcgctcatg ggagttata ctggccaac agacagctc 
        cgaaggtgg tctcagggc cttctcttc caccaagga 
        gccgtcgac tgcgcccgt gcggctctc ctcctccct 
        ctgggtatc tcaatcctc cactgccag ccggtgcag 
        aaatgctcc cttgtggaa actgccttc ctcctcctg 
        gtgacggtc gccaccgtc aacatgctg accacagtc 
        ctcaacgac tcgcccgtc ttccccgag gacgacgac 
        tccgactcc ctctccgac gtgcccctc ctcatggac 
        tcgccccaa tgcgtgctc ttcggcacg ttcatgatc 
        tggttcgcg tcgattacc atcaacttg ggccccacg 
        ttcctctcg ggggcgctg gcggcccaa ggcgaggcg 
        ggccacaag gccccctcg tgccccctc atccacggc 
        ccgctgcgc cactacatc ctcaacgcg ctctggatc 
        gccgtcaac ctggtgtgc gtctttctg acgctcttc 
        cacttgcgg aagctgcac cgcgacctc accaaggcc 
        aacgtggag gcggtgcgg gtggcgggc ctcgtcacc 
        acactggtg aacgtgaca ggaggcggc ggcgctgcc 
        gccaacggg gagcgggcc tccggcgcc ctcgaagag 
        caccgcaga atgcgagaa tacctccgt cgaatggag 
        cgcgacggc gtccagcgc gtaaagatg ttcctcgtc 
        ataaccgcc gcctacgtc cttttctgg ggtccactt 
        ttcttcgtc accctcgtc aaccacccc atcgtaggc 
        aaccctctc ggacacgag ataacgctg cacatatca 
        tacgttcat gccattgtc aatcctaca ctttttttg 
        gtactgcac agagggctc cgaagagcc acccttgat 
        ctctgttgc ggatggata ccgctgtgg ttgggcaac 
        cccaacgta cccccgccc ccagacccc cccagaact 
        gacatatcg ctgctcaaa ccacccctg cccgtacca 
        gcccaccct gatagctct atgggagac tccgatttg 
        agtccgtgg actccggag ccggtaaac ggaggaatc 
        caaatccca caatacatc agcccggag ccgagttca 
        ccacccgag agtgactag
      mRNA sequence
      • >TC012889 RA
        aatgcagtc tttcgcccc atattcaaa ttctttttt 
        agcagttgt aggaaacac acattcatt tttcgtcat 
        aacaaaggt tccgtttta aaattcaca ttccactgt 
        cgcatctcg cttctgttc ttgaaacgt gaagtgatt 
        catgaggtt tgttactga actgggtta accgaaatt 
        ccgtgacag gctctgcgg tggcgatga taggtgcca 
        acctgcgtt tcggcagtt cggtggcga atgcaagtc 
        tcaatgcca ccaaggtct tccaatgtc atcctggag 
        ggaccgctg aagccgcgc tgatattcg ccttaggaa 
        cgatgggaa tggccgcca atatagcgc tcatgggag 
        ttatactgg ccaacagac agctccgaa ggtggtctc 
        agggccttc tcttccacc aaggagccg tcgactgcg 
        cccgtgcgg ctctcctcc tccctctgg gtatctcaa 
        tcctccact gccagccgg tgcagaaat gctcccttg 
        tggaaactg ccttcctcc tcctggtga cggtcgcca 
        ccgtcaaca tgctgacca cagtcctca acgactcgc 
        ccgtcttcc ccgaggacg acgactccg actccctct 
        ccgacgtgc ccctcctca tggactcgc cccaatgcg 
        tgctcttcg gcacgttca tgatctggt tcgcgtcga 
        ttaccatca acttgggcc ccacgttcc tctcggggg 
        cgctggcgg cccaaggcg aggcgggcc acaaggccc 
        cctcgtgcc ccctcatcc acggcccgc tgcgccact 
        acatcctca acgcgctct ggatcgccg tcaacctgg 
        tgtgcgtct ttctgacgc tcttccact tgcggaagc 
        tgcaccgcg acctcacca aggccaacg tggaggcgg 
        tgcgggtgg cgggcctcg tcaccacac tggtgaacg 
        tgacaggag gcggcggcg ctgccgcca acggggagc 
        gggcctccg gcgccctcg aagagcacc gcagaatgc 
        gagaatacc tccgtcgaa tggagcgcg acggcgtcc 
        agcgcgtaa agatgttcc tcgtcataa ccgccgcct 
        acgtccttt tctggggtc cacttttct tcgtcaccc 
        tcgtcaacc accccatcg taggcaacc ctctcggac 
        acgagataa cgctgcaca tatcatacg ttcatgcca 
        ttgtcaatc ctacacttt ttttggtac tgcacagag 
        ggctccgaa gagccaccc ttgatctct gttgcggat 
        ggataccgc tgtggttgg gcaacccca acgtacccc 
        cgcccccag accccccca gaactgaca tatcgctgc 
        tcaaaccac ccctgcccg taccagccc accctgata 
        gctctatgg gagactccg atttgagtc cgtggactc 
        cggagccgg taaacggag gaatccaaa tcccacaat 
        acatcagcc cggagccga gttcaccac ccgagagtg 
        actagcatc cggattggg acagatcca cccggatct 
        aagttcgtc gtcagttaa agactgcaa tcggactct 
        tgtagtaga atcaaccga actgaactg ttttatttg 
        taaaaatta aattatgta tactgacaa gaaggtgaa 
        gagcgaaag ttttgtgca actccaacc aaaatccgc 
        ctattttag gaattgata attttaaga gactatgcg 
        agacgatta gataggaaa taaatgttg gtgactaga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY