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Gene information
General information
TC012880 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15239236..15259792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012880
    iBeetle screen iB_05348

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012880 RA
      MGQKPSTLG GRQWIFRRE HHPNPEQPI KFQYKTRRK 
      HQQERTSVG NNEVKCRIV KTQNVLRTK SEPNLSVER 
      KERHRHRRK NRIKENWGG GERFGYEIQ DVDDFLTKA 
      TIEKPANIP VVLAFPSIL YQTRVGGYQ AEIALPLGM 
      VVNAVFKNQ HWLYVQTPH AEEGYVSYA ACLPLGIIP 
      PPEDDATAP CWENSTDVF PKPSGNMTD TEKLSSKSE 
      CSVETRCHS RRLRPSSTC EERSVDRLY LSALASSKR 
      GKIQRQTLL VINSDYFGT EKNALQVQK GDVVVLQGT 
      HVPGWFWVR NKEGEEGFI PAVIAGHGF L
    CDS sequence
    • >TC012880 RA
      atgggtcag aagccatcc actctgggt ggcagacag 
      tggattttt cggcgagaa caccaccca aatccagag 
      caacccatc aagttccag tacaagacg aggcggaaa 
      caccagcag gaaaggaca agtgttgga aataatgag 
      gtgaagtgt cggattgtg aagacgcaa aatgtgcta 
      aggaccaag tcggagccg aatttgagt gtggaaagg 
      aaggagagg cacagacat cgacgcaag aacaggatc 
      aaggagaac tggggaggg ggggaacgc ttcgggtat 
      gaaatccag gacgtggac gattttctt acaaaggcc 
      acgattgaa aaaccggcc aacattccg gtcgtcttg 
      gccttcccc agcattctc taccagacg agagtcgga 
      ggataccaa gccgaaatt gccttgcct ttggggatg 
      gtggttaat gccgttttt aaaaaccag cactggttg 
      tatgtgcag acgccgcat gccgaggag gggtatgtc 
      agctatgcg gcctgttta ccactaggc attatccca 
      ccccctgag gacgacgcc accgcccct tgctgggag 
      aacagcacc gatgtgttc cctaaaccc tccggcaac 
      atgaccgac accgaaaaa ctcagctcc aagtcggaa 
      tgtagcgtc gagacgcgc tgccacagc cgaagactg 
      cgcccctca agcacctgt gaagaaaga agcgtcgat 
      cgactctac ctcagcgct ttggcatcc tcaaaacgt 
      ggcaaaatc caacgacaa acactccta gtcatcaac 
      agtgactac tttggcacc gaaaaaaat gcccttcag 
      gtgcagaaa ggcgacgtt gtggtactc cagggtacc 
      catgtgccg gggtggttc tgggtgcgc aacaaggag 
      ggagaggag gggttcata ccggcggtt atagccgga 
      cacggattt ttgtaa
    mRNA sequence
    • >TC012880 RA
      ttgccggtg ctgagcggt cgtttcttg caatctctc 
      gatctgtgc gcgattaac aaacggtga tgctttggc 
      gatgattaa attaaagtt ggacgatgg ttgattcga 
      ctcaatgac atgggtcag aagccatcc actctgggt 
      ggcagacag tggattttt cggcgagaa caccaccca 
      aatccagag caacccatc aagttccag tacaagacg 
      aggcggaaa caccagcag gaaaggaca agtgttgga 
      aataatgag gtgaagtgt cggattgtg aagacgcaa 
      aatgtgcta aggaccaag tcggagccg aatttgagt 
      gtggaaagg aaggagagg cacagacat cgacgcaag 
      aacaggatc aaggagaac tggggaggg ggggaacgc 
      ttcgggtat gaaatccag gacgtggac gattttctt 
      acaaaggcc acgattgaa aaaccggcc aacattccg 
      gtcgtcttg gccttcccc agcattctc taccagacg 
      agagtcgga ggataccaa gccgaaatt gccttgcct 
      ttggggatg gtggttaat gccgttttt aaaaaccag 
      cactggttg tatgtgcag acgccgcat gccgaggag 
      gggtatgtc agctatgcg gcctgttta ccactaggc 
      attatccca ccccctgag gacgacgcc accgcccct 
      tgctgggag aacagcacc gatgtgttc cctaaaccc 
      tccggcaac atgaccgac accgaaaaa ctcagctcc 
      aagtcggaa tgtagcgtc gagacgcgc tgccacagc 
      cgaagactg cgcccctca agcacctgt gaagaaaga 
      agcgtcgat cgactctac ctcagcgct ttggcatcc 
      tcaaaacgt ggcaaaatc caacgacaa acactccta 
      gtcatcaac agtgactac tttggcacc gaaaaaaat 
      gcccttcag gtgcagaaa ggcgacgtt gtggtactc 
      cagggtacc catgtgccg gggtggttc tgggtgcgc 
      aacaaggag ggagaggag gggttcata ccggcggtt 
      atagccgga cacggattt ttgtaaata aaagttgta 
      caaattatt tattaaaaa tttatttca aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY