| General information | |||
| TC012875 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15272538..15275172 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012875 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05347 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC012875 RA
MSYKEKLPK LVDVYRSSK PLDRRQLPL VVDENLTLV MDVRSSGLV CDNPLVRGK ELEEFTKKY DTLSETELR SALEITKSE LMNVLNQNV PCVGCRRSV ERLYYQLFK LRHPTLNPL MVEKDGKIT IDEKQAMPQ VLCSIFHDH SSRLAKLIE NQPKRSKKS LRCLLHSLD SQRSRPLTP IWRDVWGCM QTDCKKDVC IIEAPSLRA TLETYLRKH RFCGECRTK VLRAYTLLM EEAEPCKEK GYITQLYAG IKRCLRDKH IHLPIQTDY ITKLISRAE PELLGSRRE RHAKTLEIA QEEVLTCLG ICMYERLHR IYMRMREEE CTCQVFAAV AVDTLSRSF ETAVERKRG FSQLELLYA ELAREEQQK QIRKEQKKI KRKRKKGKS LEQDGKENC NEEDFEDNC YSQADLSCY SCEELLKCD DDDDDYDCD KLWIDGCKC EEEKEEKCG YTNNKKGSS DYGYSSENN NGCCETASL ISSISSSPE GSELACDTC CQHDGDTLK RFRDGEHPS LQQMLEGCS EDEDDSNCK LTPEEVWEF ENNHKHLIE KRQELREAL KKRFIDFCA YGPLPVPRL LVQSKYASN
- >TC012875 RA
atgagttat aaggaaaag ttgccaaag ctggtcgac gtttaccgc tcctcgaaa ccgctcgac aggaggcaa ttacctctt gttgtcgat gaaaattta acgttggtg atggacgtc cgcagttcc ggcctcgtg tgtgataac cccctggtc cggggcaag gagctggag gagtttacg aaaaaatac gacaccttg agcgaaacg gagctgcgt tcggcctta gaaatcacc aaatcggaa ctcatgaac gtgctcaac caaaacgtg ccatgtgtg gggtgccgc cggagcgtt gaacggctg tactaccaa ctgttcaag ctgcgccac ccgacgttg aacccccta atggtcgaa aaagacggc aaaattaca atcgacgag aagcaggcc atgccgcaa gtcctttgt tcgattttt cacgaccat tcttcacgt ttggcgaaa ttgatcgaa aatcagccg aaaagaagc aaaaagagt ctccggtgc cttttacat tcgttggat tcgcagcgt agtcgccct ttgaccccc atttggagg gacgtgtgg ggctgcatg cagactgat tgtaagaaa gatgtttgc attattgag gcccccagc ttaagggcc actctagag acgtacctg cgcaagcat aggttctgc ggcgagtgc aggacgaag gttttgaga gcttatacg cttctgatg gaagaggct gaaccttgc aaggagaaa ggttacata acccaacta tacgccggt attaagcga tgtttgcgc gacaaacac atccacctg cccatccag accgactac attacgaaa ctgattagt cgtgccgaa ccggaattg ctcggaagt cgtagagaa cgccacgct aagacttta gaaatcgcg caagaagaa gtcctcact tgtctcggc atctgcatg tatgaacgc cttcatcgt atctacatg cgtatgcgc gaggaggaa tgtacctgt caggtgttt gcagccgtg gctgttgac actttgagt cgaagtttt gaaacggcc gtcgaacga aaacgtggt ttttcacaa ctcgagtta ttatacgcg gaactagca cgcgaagaa caacagaaa cagatcaga aaagagcag aaaaagatt aaacgtaag cgcaaaaag ggcaaaagt ttggaacag gacgggaaa gagaattgc aatgaggag gatttcgag gataattgc tattcgcaa gcggatttg agttgttac agttgtgag gagttgctg aaatgtgac gatgatgat gatgattac gattgtgac aagctgtgg attgatggg tgcaagtgt gaggaggag aaggaggaa aaatgtggc tatacaaat aataaaaaa ggatcgagc gattatgga tattcgtcg gaaaataat aatggttgt tgcgaaacg gcaagtctt atttcgagt atttcaagt tcgccggaa ggatcagag ttggcgtgt gatacgtgt tgccaacat gatggcgat acgttgaag aggttcaga gatggcgaa catccgagc ttgcagcag atgttagag ggttgtagc gaggacgaa gacgacagc aattgtaaa ttaacgccg gaagaggtg tgggagttt gaaaacaac cacaaacac ttaatcgag aagcgtcaa gaattgcgc gaagcttta aaaaaacgt tttatcgat ttttgtgca tacggtcct ttgccagtg cctcgttta ctggttcag tcgaaatat gcttcaaat tga
- >TC012875 RA
tcaaaactt gtagaaaat ggtgtggta gtgcactgg attaaagtg ccgttgatt ccctgaata aaacccaaa agttcaatc gaaaaatcc cgcaagacg cttacagcc cccttggac ggagctatg agttataag gaaaagttg ccaaagctg gtcgacgtt taccgctcc tcgaaaccg ctcgacagg aggcaatta cctcttgtt gtcgatgaa aatttaacg ttggtgatg gacgtccgc agttccggc ctcgtgtgt gataacccc ctggtccgg ggcaaggag ctggaggag tttacgaaa aaatacgac accttgagc gaaacggag ctgcgttcg gccttagaa atcaccaaa tcggaactc atgaacgtg ctcaaccaa aacgtgcca tgtgtgggg tgccgccgg agcgttgaa cggctgtac taccaactg ttcaagctg cgccacccg acgttgaac cccctaatg gtcgaaaaa gacggcaaa attacaatc gacgagaag caggccatg ccgcaagtc ctttgttcg atttttcac gaccattct tcacgtttg gcgaaattg atcgaaaat cagccgaaa agaagcaaa aagagtctc cggtgcctt ttacattcg ttggattcg cagcgtagt cgccctttg acccccatt tggagggac gtgtggggc tgcatgcag actgattgt aagaaagat gtttgcatt attgaggcc cccagctta agggccact ctagagacg tacctgcgc aagcatagg ttctgcggc gagtgcagg acgaaggtt ttgagagct tatacgctt ctgatggaa gaggctgaa ccttgcaag gagaaaggt tacataacc caactatac gccggtatt aagcgatgt ttgcgcgac aaacacatc cacctgccc atccagacc gactacatt acgaaactg attagtcgt gccgaaccg gaattgctc ggaagtcgt agagaacgc cacgctaag actttagaa atcgcgcaa gaagaagtc ctcacttgt ctcggcatc tgcatgtat gaacgcctt catcgtatc tacatgcgt atgcgcgag gaggaatgt acctgtcag gtgtttgca gccgtggct gttgacact ttgagtcga agttttgaa acggccgtc gaacgaaaa cgtggtttt tcacaactc gagttatta tacgcggaa ctagcacgc gaagaacaa cagaaacag atcagaaaa gagcagaaa aagattaaa cgtaagcgc aaaaagggc aaaagtttg gaacaggac gggaaagag aattgcaat gaggaggat ttcgaggat aattgctat tcgcaagcg gatttgagt tgttacagt tgtgaggag ttgctgaaa tgtgacgat gatgatgat gattacgat tgtgacaag ctgtggatt gatgggtgc aagtgtgag gaggagaag gaggaaaaa tgtggctat acaaataat aaaaaagga tcgagcgat tatggatat tcgtcggaa aataataat ggttgttgc gaaacggca agtcttatt tcgagtatt tcaagttcg ccggaagga tcagagttg gcgtgtgat acgtgttgc caacatgat ggcgatacg ttgaagagg ttcagagat ggcgaacat ccgagcttg cagcagatg ttagagggt tgtagcgag gacgaagac gacagcaat tgtaaatta acgccggaa gaggtgtgg gagtttgaa aacaaccac aaacactta atcgagaag cgtcaagaa ttgcgcgaa gctttaaaa aaacgtttt atcgatttt tgtgcatac ggtcctttg ccagtgcct cgtttactg gttcagtcg aaatatgct tcaaattga gatttgatt ttttcattt tttattagg ggtccattt atttcgtta cagtctttt taactggat gtatttttt ttaaaacgc cgcattata caaattttt aaagcacat tattttttt tatcataca agtcagtcc agtatatgc acattccat ttatgggtt atattgtta tatattttg attagggat ggtgtgttg catcacact tgtgcagat tcagtgtat
