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Gene information
General information
TC012861 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15572688..15574175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012861
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05344

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012861 RA
      MSACDDTTR TPPPNPDVI PAKKIACAP KLFNGVEIS 
      QLTKRQMKK YQKCLKWQQ VKKEKRAKE RLKSKEKRI 
      QAKLNNVDL GPSRKQLKR MKMKDSPCK ITVCIDLSF 
      DDLMIDKDM AKTIKQILR IYTENRRAQ APMQLYLTS 
      FNGRSKTEM EKHHGFENW DLNFCPQDY LDIFTKEKL 
      VYLSSESDK VITELQQDK VYIIGGLVD HNFHKGICY 
      KKALEQGID HGQLPISEY FWLNHRKVL TINQVFEIL 
      LRVSEGKTF KAAFEMILP KRIEKISAQ DSSEEEQND 
      TTCDNK
    CDS sequence
    • >TC012861 RA
      atgagtgct tgtgatgac acaactcgg acaccgccc 
      ccaaaccct gatgtcatc ccagcgaaa aaaatcgct 
      tgtgcccca aaactcttc aacggcgtg gaaatctcg 
      caactgacc aaacgccaa atgaagaag taccaaaag 
      tgcctcaaa tggcaacaa gtcaagaag gaaaaacgg 
      gcgaaagaa cgcctcaaa agcaaggaa aagcggatt 
      caagccaag ctaaacaac gtggatttg gggcccagt 
      cgcaagcaa ctcaaacgc atgaaaatg aaagacagt 
      ccgtgtaaa atcaccgtg tgcattgac ctaagtttc 
      gacgactta atgatcgat aaagacatg gctaaaact 
      attaaacaa attttacga atttatacg gaaaatagg 
      cgcgctcag gcccccatg caactctat ttgaccagt 
      ttcaacggc cggagtaag acagaaatg gaaaaacac 
      cacggtttt gaaaactgg gaccttaat ttttgcccc 
      caagactat ttagatatt tttacgaag gaaaaactg 
      gtttattta agcagcgaa agcgataaa gtcatcacc 
      gagctacag caagacaag gtttatatc atagggggc 
      ctagttgac cataatttc cataagggt atttgctac 
      aagaaagca ctagagcaa ggaatagat cacggccaa 
      cttcccata agcgagtat ttctggcta aaccatcga 
      aaagtccta actataaat caagttttc gaaatattg 
      ctccgagtt agtgaaggc aaaacattc aaagctgcg 
      ttcgagatg atcttgccg aagagaatc gagaaaatt 
      tcggcgcag gattcgtct gaagaggaa caaaatgat 
      acaacttgt gataataaa taa
    mRNA sequence
    • >TC012861 RA
      tcatttgtc aaacgtgct aaagtgagg ttaaaaacc 
      aatattttt ttaataaaa cgttattta acgtgcaaa 
      aaccacaaa atgagtgct tgtgatgac acaactcgg 
      acaccgccc ccaaaccct gatgtcatc ccagcgaaa 
      aaaatcgct tgtgcccca aaactcttc aacggcgtg 
      gaaatctcg caactgacc aaacgccaa atgaagaag 
      taccaaaag tgcctcaaa tggcaacaa gtcaagaag 
      gaaaaacgg gcgaaagaa cgcctcaaa agcaaggaa 
      aagcggatt caagccaag ctaaacaac gtggatttg 
      gggcccagt cgcaagcaa ctcaaacgc atgaaaatg 
      aaagacagt ccgtgtaaa atcaccgtg tgcattgac 
      ctaagtttc gacgactta atgatcgat aaagacatg 
      gctaaaact attaaacaa attttacga atttatacg 
      gaaaatagg cgcgctcag gcccccatg caactctat 
      ttgaccagt ttcaacggc cggagtaag acagaaatg 
      gaaaaacac cacggtttt gaaaactgg gaccttaat 
      ttttgcccc caagactat ttagatatt tttacgaag 
      gaaaaactg gtttattta agcagcgaa agcgataaa 
      gtcatcacc gagctacag caagacaag gtttatatc 
      atagggggc ctagttgac cataatttc cataagggt 
      atttgctac aagaaagca ctagagcaa ggaatagat 
      cacggccaa cttcccata agcgagtat ttctggcta 
      aaccatcga aaagtccta actataaat caagttttc 
      gaaatattg ctccgagtt agtgaaggc aaaacattc 
      aaagctgcg ttcgagatg atcttgccg aagagaatc 
      gagaaaatt tcggcgcag gattcgtct gaagaggaa 
      caaaatgat acaacttgt gataataaa taataaatg 
      ttttgagta atgctatgt tttattaaa attcaaggg 
      cttcggatc atttgttaa aatatttgc cttccgttg 
      aaacatttc aattacctc tctgtgata tgataggaa 
      tgttattgt ttaataaaa caaacacgg caagacttg 
      tgttcagag ggataaata caaatattt ttggaaaac 
      actgtgcga ctttaaata aatttgggg gcaaagtcc 
      tttggtatt tttcaatca gtggagcga gtgttcgtg 
      ttgttgcat aagcacctg aaacaaaat gaagtgatt 
      tgaaattat aatatggcg gcgccgcca ccattttgt 
      attaaaaac cgtattatt tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY