Status
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Gene information
General information
TC011620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15578057..15581206
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011620
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05343

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    iBeetle screen iB_06536

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011620 RA
      MTVINKMEV DGGPKGEGF RSYYITKIE ELQLIVAEK 
SQNLRRLQA QRNELNAKV RMLREELML LQEQGSYVG 
EVVKPMDKK KVLVKVHPE GKFVVDIDK NIDINDVTP 
NCRVALRNE SYTLHKILP NKVDPLVSL MMVEKVPDS 
TYEMVGGLD KQIKEIKEV IELPVKHPE LFDALGIAQ 
PKGVLLYGP PGTGKTLLA RAVAHHTEC TFIRVSGSE 
LVQKFIGEG SRMVRELFV MAREHAPSI IFMDEIDSI 
GSSRIESGS GGDSEVQRT MLELLNQLD GFEATKNIK 
VIMATNRID ILDPALLRP GRIDRKIEF PPPNEEARL 
DILKIHSRK MNLTRGINL RKIAELMPG ASGAEVKGV 
CTEAGMYAL RERRVHVTQ EDFEMAVAK VMQKDSEKN 
MSIKKLWK
    CDS sequence
    • >TC011620 RA
      atgaccgtc ataaataag atggaagtg gacgggggg 
cccaaggga gagggcttc cggtcgtat tacataacg 
aaaatcgag gaattgcaa ctgattgtg gccgaaaaa 
agccagaat ttgaggcgt ctccaagcg caaagaaac 
gagctcaat gctaaagtg aggatgtta cgagaggaa 
ctaatgctg ctccaagaa cagggaagt tacgtgggc 
gaggttgtt aaacccatg gataagaaa aaagtccta 
gttaaagta caccctgaa ggtaaattt gtggttgat 
attgacaaa aacatcgac attaacgac gtgactccc 
aattgtcgc gtcgcccta cgtaatgag agctacacc 
ctgcacaaa attttgccc aacaaggtc gaccctctg 
gtatcccta atgatggtg gaaaaagtc cccgactcg 
acgtacgaa atggtcggc ggtttggac aaacagatc 
aaagagatc aaagaagtg attgaactc ccagtgaaa 
cacccggaa ttgttcgac gctttgggg atcgctcag 
cccaaggga gtgctcctg tacggccct ccgggcacc 
ggaaagacg ctcctggcc cgagctgtg gcccaccac 
accgaatgc acctttatc cgggtttct ggctcggaa 
ttggtccag aaattcatc ggggaaggg tcgcgaatg 
gtgcgcgaa ttgttcgta atggcccga gaacacgcc 
ccttcgatc atctttatg gacgagatt gattcaatc 
gggtcgtcg aggattgaa tcgggctca gggggcgat 
tctgaagtc caacgaaca atgttggaa ttgttgaat 
caattggac gggtttgaa gcaacgaaa aatatcaaa 
gttattatg gcgaccaat cgaattgat attttggat 
ccggcactc ttaaggccg gggcgtatt gatcggaaa 
attgagttt ccgccaccg aatgaagag gcgcggctt 
gatattttg aaaatacat tcgagaaaa atgaatcta 
acacgtggg attaatctg aggaaaatc gcagagctg 
atgccgggc gccagtggg gccgaagtc aagggtgtc 
tgcactgag gccgggatg tacgcctta agagagcgc 
cgcgtccac gtgactcag gaggacttc gaaatggcc 
gtagccaaa gtgatgcaa aaggattca gaaaagaac 
atgtcgatt aaaaaattg tggaaataa
    mRNA sequence
    • >TC011620 RA
      gataaatgt tccggacga aaaaaaaac aattcactt 
ataaattca tttatttaa ctaaaagcc cgctacaca 
atttttaat ttacaaatt catcttaac gtccttgaa 
ccctctgga ggcgtaatc cgagggctt ggtccgatc 
ggacagctt cggatatcc gggatcccc ctcaaccac 
ggcctccct tgatttaaa agccttcca tccgccggc 
cctcgtcca atcattggc attatactt gaagtaata 
agtgaacca ataggcggc cataacccc caagaaagc 
cttcccagt gacgtatcg cacgttcct tgcccgttc 
ttcccccaa aaccggaag caaaacctt ggccaactg 
atccaaagg ccagcggta ggccctccg gatggggtt 
gtaggtggc tttgtacat ttcgggaac cggtcgggg 
ctcacttgg ggccaactc ttggtcttt gagccattg 
tttacggaa cgctcgttc ctcgtctgt catgcccaa 
gaggcgctc gcgctcgct gttgaagcg cccggcgat 
ggacatggg cttgacgcc cccggtttg cgacgcttt 
gcccatgct ttcacacaa acgaacaca aatgaattg 
ttatataat ttggggtta ggtgtcaaa actgacagc 
tgacgtttt tgcttcaaa aaaaaaaat taattaccg 
aaattgagc ccttccctg cgccagtta cgcaagttt 
aagtgaaaa aatgcaatt tttaattta ttcaatgcg 
tgttggatt aatttaatt agaattatg ctggtttaa 
ttttttgcg attgtcggc ctgtggctg tcagctgtc 
aatattaaa acgtcactt gtgatgatt ttccaattg 
tgctttgtt tgaagatta gtggcagtt tttgagtga 
attagcgtc gtttttgtg ccgataagt tatcgcaaa 
aggccgcga aaatgaccg tcataaata agatggaag 
tggacgggg ggcccaagg gagagggct tccggtcgt 
attacataa cgaaaatcg aggaattgc aactgattg 
tggccgaaa aaagccaga atttgaggc gtctccaag 
cgcaaagaa acgagctca atgctaaag tgaggatgt 
tacgagagg aactaatgc tgctccaag aacagggaa 
gttacgtgg gcgaggttg ttaaaccca tggataaga 
aaaaagtcc tagttaaag tacaccctg aaggtaaat 
ttgtggttg atattgaca aaaacatcg acattaacg 
acgtgactc ccaattgtc gcgtcgccc tacgtaatg 
agagctaca ccctgcaca aaattttgc ccaacaagg 
tcgaccctc tggtatccc taatgatgg tggaaaaag 
tccccgact cgacgtacg aaatggtcg gcggtttgg 
acaaacaga tcaaagaga tcaaagaag tgattgaac 
tcccagtga aacacccgg aattgttcg acgctttgg 
ggatcgctc agcccaagg gagtgctcc tgtacggcc 
ctccgggca ccggaaaga cgctcctgg cccgagctg 
tggcccacc acaccgaat gcaccttta tccgggttt 
ctggctcgg aattggtcc agaaattca tcggggaag 
ggtcgcgaa tggtgcgcg aattgttcg taatggccc 
gagaacacg ccccttcga tcatcttta tggacgaga 
ttgattcaa tcgggtcgt cgaggattg aatcgggct 
cagggggcg attctgaag tccaacgaa caatgttgg 
aattgttga atcaattgg acgggtttg aagcaacga 
aaaatatca aagttatta tggcgacca atcgaattg 
atattttgg atccggcac tcttaaggc cggggcgta 
ttgatcgga aaattgagt ttccgccac cgaatgaag 
aggcgcggc ttgatattt tgaaaatac attcgagaa 
aaatgaatc taacacgtg ggattaatc tgaggaaaa 
tcgcagagc tgatgccgg gcgccagtg gggccgaag 
tcaagggtg tctgcactg aggccggga tgtacgcct 
taagagagc gccgcgtcc acgtgactc aggaggact 
tcgaaatgg ccgtagcca aagtgatgc aaaaggatt 
cagaaaaga acatgtcga ttaaaaaat tgtggaaat 
aattttatt ttaataaaa attagtaac ttga

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY