Status
Please wait ...
Gene information
General information
TC012857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15582695..15590591
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012857
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    about 50

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012857 RA
      MEQSPEREG LFDTGGLRG SVSHTPYSE ISQTRPSWT 
      RVLVLAGVA TTIGSSLPV GYNIGVVNS PAGVIKLFC 
      NQSVLNHYG VVLHEGGLD FLWSTIVAI FLVGGTIGS 
      LGGSFFADK AGRKGALIV SSLIGTIAG VCFFASKAA 
      NSFEMLIVG RLLIGVSSG LITSVMPMY LTELAPGLL 
      RGSMGVLCP LGVTCGVLL GQVLSLEGI LGNEDYWPH 
      LLAFYLLPL ASCSVILVF LPESPKYLF IIKKQPHLA 
      LKQLALIRN TKEELLDHE IQELKLEEQ DNDTEGEDW 
      SIIRVLGDK SLLLPLLLV CSLQAGQQF SGINAVFYY 
      SVSIFQKAG LSLLNSQLA TIAAGCCNL LMAIISIPV 
      MAKFNRRST LQLSLVTTT FFLIILGLA ITFISHFSW 
      MPYLSIVGV LGFVICYGI ALGPIPYFI GSELFEVGP 
      RPSAMALGS MANWGGNFI VGLCFQAML MFLGAASFF 
      IFALVVVAL FFFVRFYLP ETRGRDPVQ IAALCKRGF 
      SSRPLDSPV NSASTVDTF SVTEKEVV
    • >TC012857 RB
      MPSNEKEIS QTRPSWTRV LVLAGVATT IGSSLPVGY 
      NIGVVNSPA GVIKLFCNQ SVLNHYGVV LHEGGLDFL 
      WSTIVAIFL VGGTIGSLG GSFFADKAG RKGALIVSS 
      LIGTIAGVC FFASKAANS FEMLIVGRL LIGVSSGLI 
      TSVMPMYLT ELAPGLLRG SMGVLCPLG VTCGVLLGQ 
      VLSLEGILG NEDYWPHLL AFYLLPLAS CSVILVFLP 
      ESPKYLFII KKQPHLALK QLALIRNTK EELLDHEIQ 
      ELKLEEQDN DTEGEDWSI IRVLGDKSL LLPLLLVCS 
      LQAGQQFSG INAVFYYSV SIFQKAGLS LLNSQLATI 
      AAGCCNLLM AIISIPVMA KFNRRSTLQ LSLVTTTFF 
      LIILGLAIT FISHFSWMP YLSIVGVLG FVICYGIAL 
      GPIPYFIGS ELFEVGPRP SAMALGSMA NWGGNFIVG 
      LCFQAMLMF LGAASFFIF ALVVVALFF FVRFYLPET 
      RGRDPVQIA ALCKRGFSS RPLDSPVNS ASTVDTFSV 
      TEKEVV
    CDS sequence
    • >TC012857 RA
      atggaacag tccccagag cgtgaaggc ctgtttgac 
      acaggaggc ctcagagga agtgtttcg catacacca 
      tactcagag atctcccaa actcgccca tcatggacg 
      agggtgctt gtcctagcg ggggtggca accaccatt 
      ggttcctcc ctcccagtg ggctacaac atcggggtg 
      gtcaactct ccggcaggg gtcattaaa ttgttctgc 
      aaccaaagt gttctcaac cattatggt gtggtactt 
      cacgaaggt gggctcgat tttttgtgg tccaccatc 
      gtggctatt ttcctggtg ggtggtacc attgggtcc 
      cttggtggg tcttttttc gcggataag gccggacgc 
      aagggggcc ttaattgtg agttctctc atcggaact 
      attgccggg gtttgcttt ttcgcatcg aaagcagca 
      aactccttt gaaatgttg atcgttgga aggctcctc 
      ataggagtg tcctcaggg ttgatcacc agcgtcatg 
      cccatgtac ttaacggaa ttggcccca ggtttatta 
      agggggtcc atgggggtg ttgtgcccc cttggcgtg 
      acttgtggt gtcctattg gggcaagtt ttgtcctta 
      gaagggatt ttagggaat gaggattat tggccccat 
      ttgttggcc ttctatttg ctacctctt gcttcatgt 
      agtgtgata ttagtattt ttgcctgaa agtccaaag 
      tatcttttt ataatcaaa aagcaacca catctagcg 
      ctaaaacaa ttagcactt attcgcaac accaaagaa 
      gagctgctc gatcacgaa atccaagaa ctgaagctt 
      gaggaacaa gataacgac acagagggt gaagattgg 
      agcataatt cgtgtcctt ggtgataaa tctttgcta 
      ctaccactg ctcctagtt tgtagtctt caagctggg 
      caacaattt agcggaatc aacgctgtt ttttactac 
      tcggtttca atcttccaa aaagctggg ctttcgtta 
      ctaaacagc caactcgcg actattgca gctggctgt 
      tgcaacctc ctcatggcc ataatttca atccctgtg 
      atggcaaaa ttcaaccgc cgaagcacc cttcagttg 
      agtctagtg accacaact tttttccta attattcta 
      ggactcgcc attactttt atttcccac ttctcctgg 
      atgccctat ttgagcatc gtcggagtt ttaggtttc 
      gtgatttgc tacggaatc gcccttggc cctatccct 
      tatttcatc ggatccgag ctgtttgaa gtggggcca 
      cgacctagt gccatggcc cttggcagt atggccaac 
      tggggcggg aattttatc gtcggtttg tgttttcag 
      gccatgttg atgtttctg ggggccgct tcgtttttt 
      atttttgcc cttgttgtc gtcgctttg ttctttttt 
      gtcaggttt tatttgccc gaaacgaga ggacgggac 
      ccagtccaa atcgcagct ttgtgcaaa aggggtttt 
      tcctcaagg cctttagat tctccggtc aattcagcg 
      agtactgtc gatactttt tcagtgact gagaaggag 
      gtggtttga
    • >TC012857 RB
      atgccatcc aatgagaag gagatctcc caaactcgc 
      ccatcatgg acgagggtg cttgtccta gcgggggtg 
      gcaaccacc attggttcc tccctccca gtgggctac 
      aacatcggg gtggtcaac tctccggca ggggtcatt 
      aaattgttc tgcaaccaa agtgttctc aaccattat 
      ggtgtggta cttcacgaa ggtgggctc gattttttg 
      tggtccacc atcgtggct attttcctg gtgggtggt 
      accattggg tcccttggt gggtctttt ttcgcggat 
      aaggccgga cgcaagggg gccttaatt gtgagttct 
      ctcatcgga actattgcc ggggtttgc tttttcgca 
      tcgaaagca gcaaactcc tttgaaatg ttgatcgtt 
      ggaaggctc ctcatagga gtgtcctca gggttgatc 
      accagcgtc atgcccatg tacttaacg gaattggcc 
      ccaggttta ttaaggggg tccatgggg gtgttgtgc 
      ccccttggc gtgacttgt ggtgtccta ttggggcaa 
      gttttgtcc ttagaaggg attttaggg aatgaggat 
      tattggccc catttgttg gccttctat ttgctacct 
      cttgcttca tgtagtgtg atattagta tttttgcct 
      gaaagtcca aagtatctt tttataatc aaaaagcaa 
      ccacatcta gcgctaaaa caattagca cttattcgc 
      aacaccaaa gaagagctg ctcgatcac gaaatccaa 
      gaactgaag cttgaggaa caagataac gacacagag 
      ggtgaagat tggagcata attcgtgtc cttggtgat 
      aaatctttg ctactacca ctgctccta gtttgtagt 
      cttcaagct gggcaacaa tttagcgga atcaacgct 
      gttttttac tactcggtt tcaatcttc caaaaagct 
      gggctttcg ttactaaac agccaactc gcgactatt 
      gcagctggc tgttgcaac ctcctcatg gccataatt 
      tcaatccct gtgatggca aaattcaac cgccgaagc 
      acccttcag ttgagtcta gtgaccaca acttttttc 
      ctaattatt ctaggactc gccattact tttatttcc 
      cacttctcc tggatgccc tatttgagc atcgtcgga 
      gttttaggt ttcgtgatt tgctacgga atcgccctt 
      ggccctatc ccttatttc atcggatcc gagctgttt 
      gaagtgggg ccacgacct agtgccatg gcccttggc 
      agtatggcc aactggggc gggaatttt atcgtcggt 
      ttgtgtttt caggccatg ttgatgttt ctgggggcc 
      gcttcgttt tttattttt gcccttgtt gtcgtcgct 
      ttgttcttt tttgtcagg ttttatttg cccgaaacg 
      agaggacgg gacccagtc caaatcgca gctttgtgc 
      aaaaggggt ttttcctca aggccttta gattctccg 
      gtcaattca gcgagtact gtcgatact ttttcagtg 
      actgagaag gaggtggtt tga
    mRNA sequence
    • >TC012857 RA
      cctcgcaag ttctgataa agtaacaga tggtagcag 
      ctacttcca gactcattt ttgagttta ttgagttgc 
      gcgttacac gaaaatagg ccgcacacc tgcatacag 
      cagcagatg tctacaaat ttattatta gtgacgttt 
      taagtcact agtgtcata aaaaatggt gtaaaacag 
      tgacaatta atttttttc aataaatat acaattgat 
      cgaactgag agtcgtatg tcatgtact acgcttcgg 
      agctaattc cggcaaagt ttctcgttt tagttgccc 
      attttacca tttgtgtat tattgttat ggaacagtc 
      cccagagcg tgaaggcct gtttgacac aggaggcct 
      cagaggaag tgtttcgca tacaccata ctcagagat 
      ctcccaaac tcgcccatc atggacgag ggtgcttgt 
      cctagcggg ggtggcaac caccattgg ttcctccct 
      cccagtggg ctacaacat cggggtggt caactctcc 
      ggcaggggt cattaaatt gttctgcaa ccaaagtgt 
      tctcaacca ttatggtgt ggtacttca cgaaggtgg 
      gctcgattt tttgtggtc caccatcgt ggctatttt 
      cctggtggg tggtaccat tgggtccct tggtgggtc 
      ttttttcgc ggataaggc cggacgcaa gggggcctt 
      aattgtgag ttctctcat cggaactat tgccggggt 
      ttgcttttt cgcatcgaa agcagcaaa ctcctttga 
      aatgttgat cgttggaag gctcctcat aggagtgtc 
      ctcagggtt gatcaccag cgtcatgcc catgtactt 
      aacggaatt ggccccagg tttattaag ggggtccat 
      gggggtgtt gtgccccct tggcgtgac ttgtggtgt 
      cctattggg gcaagtttt gtccttaga agggatttt 
      agggaatga ggattattg gccccattt gttggcctt 
      ctatttgct acctcttgc ttcatgtag tgtgatatt 
      agtattttt gcctgaaag tccaaagta tctttttat 
      aatcaaaaa gcaaccaca tctagcgct aaaacaatt 
      agcacttat tcgcaacac caaagaaga gctgctcga 
      tcacgaaat ccaagaact gaagcttga ggaacaaga 
      taacgacac agagggtga agattggag cataattcg 
      tgtccttgg tgataaatc tttgctact accactgct 
      cctagtttg tagtcttca agctgggca acaatttag 
      cggaatcaa cgctgtttt ttactactc ggtttcaat 
      cttccaaaa agctgggct ttcgttact aaacagcca 
      actcgcgac tattgcagc tggctgttg caacctcct 
      catggccat aatttcaat ccctgtgat ggcaaaatt 
      caaccgccg aagcaccct tcagttgag tctagtgac 
      cacaacttt tttcctaat tattctagg actcgccat 
      tacttttat ttcccactt ctcctggat gccctattt 
      gagcatcgt cggagtttt aggtttcgt gatttgcta 
      cggaatcgc ccttggccc tatccctta tttcatcgg 
      atccgagct gtttgaagt ggggccacg acctagtgc 
      catggccct tggcagtat ggccaactg gggcgggaa 
      ttttatcgt cggtttgtg ttttcaggc catgttgat 
      gtttctggg ggccgcttc gttttttat ttttgccct 
      tgttgtcgt cgctttgtt cttttttgt caggtttta 
      tttgcccga aacgagagg acgggaccc agtccaaat 
      cgcagcttt gtgcaaaag gggtttttc ctcaaggcc 
      tttagattc tccggtcaa ttcagcgag tactgtcga 
      tactttttc agtgactga gaaggaggt ggtttgagg 
      aggtgttta aagccatgt cttccattt taataaatt 
      ttttaacaa aga
    • >TC012857 RB
      attcatttg aagccatgc catccaatg agaaggaga 
      tctcccaaa ctcgcccat catggacga gggtgcttg 
      tcctagcgg gggtggcaa ccaccattg gttcctccc 
      tcccagtgg gctacaaca tcggggtgg tcaactctc 
      cggcagggg tcattaaat tgttctgca accaaagtg 
      ttctcaacc attatggtg tggtacttc acgaaggtg 
      ggctcgatt ttttgtggt ccaccatcg tggctattt 
      tcctggtgg gtggtacca ttgggtccc ttggtgggt 
      cttttttcg cggataagg ccggacgca agggggcct 
      taattgtga gttctctca tcggaacta ttgccgggg 
      tttgctttt tcgcatcga aagcagcaa actcctttg 
      aaatgttga tcgttggaa ggctcctca taggagtgt 
      cctcagggt tgatcacca gcgtcatgc ccatgtact 
      taacggaat tggccccag gtttattaa gggggtcca 
      tgggggtgt tgtgccccc ttggcgtga cttgtggtg 
      tcctattgg ggcaagttt tgtccttag aagggattt 
      tagggaatg aggattatt ggccccatt tgttggcct 
      tctatttgc tacctcttg cttcatgta gtgtgatat 
      tagtatttt tgcctgaaa gtccaaagt atcttttta 
      taatcaaaa agcaaccac atctagcgc taaaacaat 
      tagcactta ttcgcaaca ccaaagaag agctgctcg 
      atcacgaaa tccaagaac tgaagcttg aggaacaag 
      ataacgaca cagagggtg aagattgga gcataattc 
      gtgtccttg gtgataaat ctttgctac taccactgc 
      tcctagttt gtagtcttc aagctgggc aacaattta 
      gcggaatca acgctgttt tttactact cggtttcaa 
      tcttccaaa aagctgggc tttcgttac taaacagcc 
      aactcgcga ctattgcag ctggctgtt gcaacctcc 
      tcatggcca taatttcaa tccctgtga tggcaaaat 
      tcaaccgcc gaagcaccc ttcagttga gtctagtga 
      ccacaactt ttttcctaa ttattctag gactcgcca 
      ttactttta tttcccact tctcctgga tgccctatt 
      tgagcatcg tcggagttt taggtttcg tgatttgct 
      acggaatcg cccttggcc ctatccctt atttcatcg 
      gatccgagc tgtttgaag tggggccac gacctagtg 
      ccatggccc ttggcagta tggccaact ggggcggga 
      attttatcg tcggtttgt gttttcagg ccatgttga 
      tgtttctgg gggccgctt cgtttttta tttttgccc 
      ttgttgtcg tcgctttgt tcttttttg tcaggtttt 
      atttgcccg aaacgagag gacgggacc cagtccaaa 
      tcgcagctt tgtgcaaaa ggggttttt cctcaaggc 
      ctttagatt ctccggtca attcagcga gtactgtcg 
      atacttttt cagtgactg agaaggagg tggtttgag 
      gaggtgttt aaagccatg tcttccatt ttaataaat 
      tttttaaca aaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY