Status
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Gene information
General information
TC012856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15596707..15598731
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012856
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Adult morphology
    antenna distal pigmentation/tanning lighter - Number of animals showing the phenotype: 3

    Fertility
    egg production: number <50% of expected -
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012856 RA
      MKRLILSYF VFAPIFANM SPTVLTNSG RVQGRISTF 
      YAFKGIPYA KPPLGKLRF QPPQPPEPW KGTKICDKY 
      GPKCLQIDK NDGSMTGNE DCLTLNVFT RDLTNPSPV 
      MVYFHGGAH LRGSGADLG PEYLMEKSV VLVTVNFRL 
      NVFGFLTTC DENAFGNAG IKDQVRALE WVQENIAGF 
      GGDPGNVTI FGESSGADS VSLLLLSPR TKGLFHRVI 
      LQSGSSLNP RYLQRNPLP FVVNLAKYF GIRCENTKS 
      IVEGLQEID GSELLKAAN EAKATGYQL DLRAAAFYP 
      VIEAENKDA IITQSPYEL FQSGNFNKV PFIIGYNSN 
      EGTLGLNLV NNMNITDFD SNYELFYPQ SLNNANKSE 
      EIGKIVREY FLGNDSAQN TDKFAKFLA QELLVRGIR 
      KNVDFLIKH SEGYFYKFT YKGTHTSQY PGVGHAEEL 
      QYIFTKNGT TRNLTQSDV LMRKRMVEM WTNFANFGA 
      PVPGQRLLG ETISWEKCE EKITYLEIG ENLELKENP 
      DREEYEFWE KLFKDYGKP PFQTY
    CDS sequence
    • >TC012856 RA
      atgaaacga ttaatactc agttacttc gttttcgcc 
      ccaattttc gcaaacatg tccccaacc gtgctcaca 
      aactctggg cgcgtccag ggacgtatt tccacattt 
      tacgccttt aaaggaata ccgtacgcg aaaccaccc 
      cttggaaaa ctgcgcttc caaccaccg caaccgcca 
      gaaccctgg aaagggacc aaaatctgc gataaatac 
      ggccccaag tgcctccaa attgacaaa aacgatggt 
      tcaatgacc ggaaacgag gactgttta accctcaat 
      gttttcact cgtgattta acaaaccca tcccctgtg 
      atggtctat ttccatgga ggggcccat ctcaggggg 
      tcgggggcc gacttgggg cccgaatac ctcatggag 
      aagtcggtg gtgctggtg actgttaat ttccggctg 
      aatgttttc ggttttttg accacttgt gacgaaaac 
      gcgtttgga aacgcaggg attaaggac caagtgagg 
      gctttagag tgggtccag gagaatatt gctgggttt 
      gggggggat cccggaaat gtgaccatt tttggggag 
      agttcaggg gcggactcg gtgtctttg ctacttttg 
      agcccgaga acaaagggc ttgtttcat cgggtgatt 
      ttgcaaagt gggtcgagt cttaatccc aggtatttg 
      caacgaaat cccttgcct tttgtggtc aatttggcc 
      aaatatttt ggcattcgt tgcgaaaat accaaaagt 
      atagttgag gggttgcag gaaattgac gggagtgag 
      ttactcaaa gctgcaaat gaggccaaa gctacgggc 
      taccaactt gatctgaga gctgcggcc ttttatccg 
      gtcattgaa gccgaaaat aaggacgcg atcataacg 
      caaagtccc tacgaactg ttccaaagt ggaaatttc 
      aataaagta ccgttcata attggctat aattccaac 
      gagggcact ttgggacta aacctcgtc aataacatg 
      aatatcacc gattttgac tcaaactac gagttgttt 
      tatccacaa tccttgaat aacgccaac aagagcgaa 
      gaaataggg aaaatagtc agggagtat tttttggga 
      aacgattcg gcacaaaac accgacaaa ttcgcaaag 
      tttttggca caggaactt ctagtgcga ggcatccgc 
      aaaaacgtt gattttttg atcaaacac tcggagggc 
      tatttttac aaattcacc tacaaaggc acccacacg 
      agtcaatac ccaggtgtg ggacatgcc gaagagttg 
      cagtatatt tttaccaaa aatgggacg actaggaat 
      ttgactcag agtgatgtt ttgatgcgg aaacggatg 
      gttgagatg tggacgaat tttgcaaat tttggggcc 
      ccagttccc ggacaacga ctcttgggc gagacaatt 
      tcgtgggaa aagtgtgag gagaaaatt acttatctc 
      gaaattggg gaaaatttg gaactgaag gagaatccg 
      gatcgggaa gaatacgaa ttttgggaa aaattgttc 
      aaggattat ggaaaacca ccctttcag acgtactaa
    mRNA sequence
    • >TC012856 RA
      agtcgtata tttgatata aaaacaaac aaaagcgaa 
      caaaggaaa cgttttttt gtgataaca ttaaaacaa 
      tgaaacgat taatactca gttacttcg ttttcgccc 
      caattttcg caaacatgt ccccaaccg tgctcacaa 
      actctgggc gcgtccagg gacgtattt ccacatttt 
      acgccttta aaggaatac cgtacgcga aaccacccc 
      ttggaaaac tgcgcttcc aaccaccgc aaccgccag 
      aaccctgga aagggacca aaatctgcg ataaatacg 
      gccccaagt gcctccaaa ttgacaaaa acgatggtt 
      caatgaccg gaaacgagg actgtttaa ccctcaatg 
      ttttcactc gtgatttaa caaacccat cccctgtga 
      tggtctatt tccatggag gggcccatc tcagggggt 
      cgggggccg acttggggc ccgaatacc tcatggaga 
      agtcggtgg tgctggtga ctgttaatt tccggctga 
      atgttttcg gttttttga ccacttgtg acgaaaacg 
      cgtttggaa acgcaggga ttaaggacc aagtgaggg 
      ctttagagt gggtccagg agaatattg ctgggtttg 
      ggggggatc ccggaaatg tgaccattt ttggggaga 
      gttcagggg cggactcgg tgtctttgc tacttttga 
      gcccgagaa caaagggct tgtttcatc gggtgattt 
      tgcaaagtg ggtcgagtc ttaatccca ggtatttgc 
      aacgaaatc ccttgcctt ttgtggtca atttggcca 
      aatattttg gcattcgtt gcgaaaata ccaaaagta 
      tagttgagg ggttgcagg aaattgacg ggagtgagt 
      tactcaaag ctgcaaatg aggccaaag ctacgggct 
      accaacttg atctgagag ctgcggcct tttatccgg 
      tcattgaag ccgaaaata aggacgcga tcataacgc 
      aaagtccct acgaactgt tccaaagtg gaaatttca 
      ataaagtac cgttcataa ttggctata attccaacg 
      agggcactt tgggactaa acctcgtca ataacatga 
      atatcaccg attttgact caaactacg agttgtttt 
      atccacaat ccttgaata acgccaaca agagcgaag 
      aaataggga aaatagtca gggagtatt ttttgggaa 
      acgattcgg cacaaaaca ccgacaaat tcgcaaagt 
      ttttggcac aggaacttc tagtgcgag gcatccgca 
      aaaacgttg attttttga tcaaacact cggagggct 
      atttttaca aattcacct acaaaggca cccacacga 
      gtcaatacc caggtgtgg gacatgccg aagagttgc 
      agtatattt ttaccaaaa atgggacga ctaggaatt 
      tgactcaga gtgatgttt tgatgcgga aacggatgg 
      ttgagatgt ggacgaatt ttgcaaatt ttggggccc 
      cagttcccg gacaacgac tcttgggcg agacaattt 
      cgtgggaaa agtgtgagg agaaaatta cttatctcg 
      aaattgggg aaaatttgg aactgaagg agaatccgg 
      atcgggaag aatacgaat tttgggaaa aattgttca 
      aggattatg gaaaaccac cctttcaga cgtactaat 
      gataataaa agaaaaatt atttgtatc agtgtgaaa 
      atctaattt ttttatatt gtctcctct ttttgtaaa 
      ataaaaact tattgagtt tgtgtctcc caaaaaatc 
      ctatattaa ataattcaa taaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY