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Gene information
General information
TC012856 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15596707..15598731
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012856
    iBeetle screen iB_05341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Adult morphology
    antenna distal pigmentation/tanning lighter - Number of animals showing the phenotype: 3

    Fertility
    egg production: number <50% of expected -
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012856 RA
      MKRLILSYF VFAPIFANM SPTVLTNSG RVQGRISTF 
YAFKGIPYA KPPLGKLRF QPPQPPEPW KGTKICDKY 
GPKCLQIDK NDGSMTGNE DCLTLNVFT RDLTNPSPV 
MVYFHGGAH LRGSGADLG PEYLMEKSV VLVTVNFRL 
NVFGFLTTC DENAFGNAG IKDQVRALE WVQENIAGF 
GGDPGNVTI FGESSGADS VSLLLLSPR TKGLFHRVI 
LQSGSSLNP RYLQRNPLP FVVNLAKYF GIRCENTKS 
IVEGLQEID GSELLKAAN EAKATGYQL DLRAAAFYP 
VIEAENKDA IITQSPYEL FQSGNFNKV PFIIGYNSN 
EGTLGLNLV NNMNITDFD SNYELFYPQ SLNNANKSE 
EIGKIVREY FLGNDSAQN TDKFAKFLA QELLVRGIR 
KNVDFLIKH SEGYFYKFT YKGTHTSQY PGVGHAEEL 
QYIFTKNGT TRNLTQSDV LMRKRMVEM WTNFANFGA 
PVPGQRLLG ETISWEKCE EKITYLEIG ENLELKENP 
DREEYEFWE KLFKDYGKP PFQTY
    CDS sequence
    • >TC012856 RA
      atgaaacga ttaatactc agttacttc gttttcgcc 
ccaattttc gcaaacatg tccccaacc gtgctcaca 
aactctggg cgcgtccag ggacgtatt tccacattt 
tacgccttt aaaggaata ccgtacgcg aaaccaccc 
cttggaaaa ctgcgcttc caaccaccg caaccgcca 
gaaccctgg aaagggacc aaaatctgc gataaatac 
ggccccaag tgcctccaa attgacaaa aacgatggt 
tcaatgacc ggaaacgag gactgttta accctcaat 
gttttcact cgtgattta acaaaccca tcccctgtg 
atggtctat ttccatgga ggggcccat ctcaggggg 
tcgggggcc gacttgggg cccgaatac ctcatggag 
aagtcggtg gtgctggtg actgttaat ttccggctg 
aatgttttc ggttttttg accacttgt gacgaaaac 
gcgtttgga aacgcaggg attaaggac caagtgagg 
gctttagag tgggtccag gagaatatt gctgggttt 
gggggggat cccggaaat gtgaccatt tttggggag 
agttcaggg gcggactcg gtgtctttg ctacttttg 
agcccgaga acaaagggc ttgtttcat cgggtgatt 
ttgcaaagt gggtcgagt cttaatccc aggtatttg 
caacgaaat cccttgcct tttgtggtc aatttggcc 
aaatatttt ggcattcgt tgcgaaaat accaaaagt 
atagttgag gggttgcag gaaattgac gggagtgag 
ttactcaaa gctgcaaat gaggccaaa gctacgggc 
taccaactt gatctgaga gctgcggcc ttttatccg 
gtcattgaa gccgaaaat aaggacgcg atcataacg 
caaagtccc tacgaactg ttccaaagt ggaaatttc 
aataaagta ccgttcata attggctat aattccaac 
gagggcact ttgggacta aacctcgtc aataacatg 
aatatcacc gattttgac tcaaactac gagttgttt 
tatccacaa tccttgaat aacgccaac aagagcgaa 
gaaataggg aaaatagtc agggagtat tttttggga 
aacgattcg gcacaaaac accgacaaa ttcgcaaag 
tttttggca caggaactt ctagtgcga ggcatccgc 
aaaaacgtt gattttttg atcaaacac tcggagggc 
tatttttac aaattcacc tacaaaggc acccacacg 
agtcaatac ccaggtgtg ggacatgcc gaagagttg 
cagtatatt tttaccaaa aatgggacg actaggaat 
ttgactcag agtgatgtt ttgatgcgg aaacggatg 
gttgagatg tggacgaat tttgcaaat tttggggcc 
ccagttccc ggacaacga ctcttgggc gagacaatt 
tcgtgggaa aagtgtgag gagaaaatt acttatctc 
gaaattggg gaaaatttg gaactgaag gagaatccg 
gatcgggaa gaatacgaa ttttgggaa aaattgttc 
aaggattat ggaaaacca ccctttcag acgtactaa
    mRNA sequence
    • >TC012856 RA
      agtcgtata tttgatata aaaacaaac aaaagcgaa 
caaaggaaa cgttttttt gtgataaca ttaaaacaa 
tgaaacgat taatactca gttacttcg ttttcgccc 
caattttcg caaacatgt ccccaaccg tgctcacaa 
actctgggc gcgtccagg gacgtattt ccacatttt 
acgccttta aaggaatac cgtacgcga aaccacccc 
ttggaaaac tgcgcttcc aaccaccgc aaccgccag 
aaccctgga aagggacca aaatctgcg ataaatacg 
gccccaagt gcctccaaa ttgacaaaa acgatggtt 
caatgaccg gaaacgagg actgtttaa ccctcaatg 
ttttcactc gtgatttaa caaacccat cccctgtga 
tggtctatt tccatggag gggcccatc tcagggggt 
cgggggccg acttggggc ccgaatacc tcatggaga 
agtcggtgg tgctggtga ctgttaatt tccggctga 
atgttttcg gttttttga ccacttgtg acgaaaacg 
cgtttggaa acgcaggga ttaaggacc aagtgaggg 
ctttagagt gggtccagg agaatattg ctgggtttg 
ggggggatc ccggaaatg tgaccattt ttggggaga 
gttcagggg cggactcgg tgtctttgc tacttttga 
gcccgagaa caaagggct tgtttcatc gggtgattt 
tgcaaagtg ggtcgagtc ttaatccca ggtatttgc 
aacgaaatc ccttgcctt ttgtggtca atttggcca 
aatattttg gcattcgtt gcgaaaata ccaaaagta 
tagttgagg ggttgcagg aaattgacg ggagtgagt 
tactcaaag ctgcaaatg aggccaaag ctacgggct 
accaacttg atctgagag ctgcggcct tttatccgg 
tcattgaag ccgaaaata aggacgcga tcataacgc 
aaagtccct acgaactgt tccaaagtg gaaatttca 
ataaagtac cgttcataa ttggctata attccaacg 
agggcactt tgggactaa acctcgtca ataacatga 
atatcaccg attttgact caaactacg agttgtttt 
atccacaat ccttgaata acgccaaca agagcgaag 
aaataggga aaatagtca gggagtatt ttttgggaa 
acgattcgg cacaaaaca ccgacaaat tcgcaaagt 
ttttggcac aggaacttc tagtgcgag gcatccgca 
aaaacgttg attttttga tcaaacact cggagggct 
atttttaca aattcacct acaaaggca cccacacga 
gtcaatacc caggtgtgg gacatgccg aagagttgc 
agtatattt ttaccaaaa atgggacga ctaggaatt 
tgactcaga gtgatgttt tgatgcgga aacggatgg 
ttgagatgt ggacgaatt ttgcaaatt ttggggccc 
cagttcccg gacaacgac tcttgggcg agacaattt 
cgtgggaaa agtgtgagg agaaaatta cttatctcg 
aaattgggg aaaatttgg aactgaagg agaatccgg 
atcgggaag aatacgaat tttgggaaa aattgttca 
aggattatg gaaaaccac cctttcaga cgtactaat 
gataataaa agaaaaatt atttgtatc agtgtgaaa 
atctaattt ttttatatt gtctcctct ttttgtaaa 
ataaaaact tattgagtt tgtgtctcc caaaaaatc 
ctatattaa ataattcaa taaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY