| General information | |||
| TC012856 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 15596707..15598731 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012856 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05341 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
fertility after larval injection decreased (no living offspring) -
- >TC012856 RA
MKRLILSYF VFAPIFANM SPTVLTNSG RVQGRISTF YAFKGIPYA KPPLGKLRF QPPQPPEPW KGTKICDKY GPKCLQIDK NDGSMTGNE DCLTLNVFT RDLTNPSPV MVYFHGGAH LRGSGADLG PEYLMEKSV VLVTVNFRL NVFGFLTTC DENAFGNAG IKDQVRALE WVQENIAGF GGDPGNVTI FGESSGADS VSLLLLSPR TKGLFHRVI LQSGSSLNP RYLQRNPLP FVVNLAKYF GIRCENTKS IVEGLQEID GSELLKAAN EAKATGYQL DLRAAAFYP VIEAENKDA IITQSPYEL FQSGNFNKV PFIIGYNSN EGTLGLNLV NNMNITDFD SNYELFYPQ SLNNANKSE EIGKIVREY FLGNDSAQN TDKFAKFLA QELLVRGIR KNVDFLIKH SEGYFYKFT YKGTHTSQY PGVGHAEEL QYIFTKNGT TRNLTQSDV LMRKRMVEM WTNFANFGA PVPGQRLLG ETISWEKCE EKITYLEIG ENLELKENP DREEYEFWE KLFKDYGKP PFQTY
- >TC012856 RA
atgaaacga ttaatactc agttacttc gttttcgcc ccaattttc gcaaacatg tccccaacc gtgctcaca aactctggg cgcgtccag ggacgtatt tccacattt tacgccttt aaaggaata ccgtacgcg aaaccaccc cttggaaaa ctgcgcttc caaccaccg caaccgcca gaaccctgg aaagggacc aaaatctgc gataaatac ggccccaag tgcctccaa attgacaaa aacgatggt tcaatgacc ggaaacgag gactgttta accctcaat gttttcact cgtgattta acaaaccca tcccctgtg atggtctat ttccatgga ggggcccat ctcaggggg tcgggggcc gacttgggg cccgaatac ctcatggag aagtcggtg gtgctggtg actgttaat ttccggctg aatgttttc ggttttttg accacttgt gacgaaaac gcgtttgga aacgcaggg attaaggac caagtgagg gctttagag tgggtccag gagaatatt gctgggttt gggggggat cccggaaat gtgaccatt tttggggag agttcaggg gcggactcg gtgtctttg ctacttttg agcccgaga acaaagggc ttgtttcat cgggtgatt ttgcaaagt gggtcgagt cttaatccc aggtatttg caacgaaat cccttgcct tttgtggtc aatttggcc aaatatttt ggcattcgt tgcgaaaat accaaaagt atagttgag gggttgcag gaaattgac gggagtgag ttactcaaa gctgcaaat gaggccaaa gctacgggc taccaactt gatctgaga gctgcggcc ttttatccg gtcattgaa gccgaaaat aaggacgcg atcataacg caaagtccc tacgaactg ttccaaagt ggaaatttc aataaagta ccgttcata attggctat aattccaac gagggcact ttgggacta aacctcgtc aataacatg aatatcacc gattttgac tcaaactac gagttgttt tatccacaa tccttgaat aacgccaac aagagcgaa gaaataggg aaaatagtc agggagtat tttttggga aacgattcg gcacaaaac accgacaaa ttcgcaaag tttttggca caggaactt ctagtgcga ggcatccgc aaaaacgtt gattttttg atcaaacac tcggagggc tatttttac aaattcacc tacaaaggc acccacacg agtcaatac ccaggtgtg ggacatgcc gaagagttg cagtatatt tttaccaaa aatgggacg actaggaat ttgactcag agtgatgtt ttgatgcgg aaacggatg gttgagatg tggacgaat tttgcaaat tttggggcc ccagttccc ggacaacga ctcttgggc gagacaatt tcgtgggaa aagtgtgag gagaaaatt acttatctc gaaattggg gaaaatttg gaactgaag gagaatccg gatcgggaa gaatacgaa ttttgggaa aaattgttc aaggattat ggaaaacca ccctttcag acgtactaa
- >TC012856 RA
agtcgtata tttgatata aaaacaaac aaaagcgaa caaaggaaa cgttttttt gtgataaca ttaaaacaa tgaaacgat taatactca gttacttcg ttttcgccc caattttcg caaacatgt ccccaaccg tgctcacaa actctgggc gcgtccagg gacgtattt ccacatttt acgccttta aaggaatac cgtacgcga aaccacccc ttggaaaac tgcgcttcc aaccaccgc aaccgccag aaccctgga aagggacca aaatctgcg ataaatacg gccccaagt gcctccaaa ttgacaaaa acgatggtt caatgaccg gaaacgagg actgtttaa ccctcaatg ttttcactc gtgatttaa caaacccat cccctgtga tggtctatt tccatggag gggcccatc tcagggggt cgggggccg acttggggc ccgaatacc tcatggaga agtcggtgg tgctggtga ctgttaatt tccggctga atgttttcg gttttttga ccacttgtg acgaaaacg cgtttggaa acgcaggga ttaaggacc aagtgaggg ctttagagt gggtccagg agaatattg ctgggtttg ggggggatc ccggaaatg tgaccattt ttggggaga gttcagggg cggactcgg tgtctttgc tacttttga gcccgagaa caaagggct tgtttcatc gggtgattt tgcaaagtg ggtcgagtc ttaatccca ggtatttgc aacgaaatc ccttgcctt ttgtggtca atttggcca aatattttg gcattcgtt gcgaaaata ccaaaagta tagttgagg ggttgcagg aaattgacg ggagtgagt tactcaaag ctgcaaatg aggccaaag ctacgggct accaacttg atctgagag ctgcggcct tttatccgg tcattgaag ccgaaaata aggacgcga tcataacgc aaagtccct acgaactgt tccaaagtg gaaatttca ataaagtac cgttcataa ttggctata attccaacg agggcactt tgggactaa acctcgtca ataacatga atatcaccg attttgact caaactacg agttgtttt atccacaat ccttgaata acgccaaca agagcgaag aaataggga aaatagtca gggagtatt ttttgggaa acgattcgg cacaaaaca ccgacaaat tcgcaaagt ttttggcac aggaacttc tagtgcgag gcatccgca aaaacgttg attttttga tcaaacact cggagggct atttttaca aattcacct acaaaggca cccacacga gtcaatacc caggtgtgg gacatgccg aagagttgc agtatattt ttaccaaaa atgggacga ctaggaatt tgactcaga gtgatgttt tgatgcgga aacggatgg ttgagatgt ggacgaatt ttgcaaatt ttggggccc cagttcccg gacaacgac tcttgggcg agacaattt cgtgggaaa agtgtgagg agaaaatta cttatctcg aaattgggg aaaatttgg aactgaagg agaatccgg atcgggaag aatacgaat tttgggaaa aattgttca aggattatg gaaaaccac cctttcaga cgtactaat gataataaa agaaaaatt atttgtatc agtgtgaaa atctaattt ttttatatt gtctcctct ttttgtaaa ataaaaact tattgagtt tgtgtctcc caaaaaatc ctatattaa ataattcaa taaaaa
