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Gene information
General information
TC012851 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15627847..15629521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012851
    iBeetle screen iB_05339

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    about 50

    gnathal appendages mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012851 RA
      MVTGTNNTP VQPNAIPTT NNQIEMAAA TKRVSDNRR 
      SNKPIMEKR RRARINNSL NELKTLILD AMKKDPARH 
      SKLEKADIL EMTVKHLQN LQRQQAAMS AATDPSVVS 
      KFRAGFSEC ASEVGRFPG LDPVVKRRL LQHLASCLN 
      QGQKEPQVQ VIVPEVAPN NIILGNGTG VQLVPTRLA 
      NGDIALVLP TQGASPLPL LVPIPQRTA STASSASNY 
      SPSQSPEPE SVRPLSLVV RRCEEPTEE KPWRPW
    CDS sequence
    • >TC012851 RA
      atggttacg ggaaccaac aacacccca gtgcaaccc 
      aatgccatt ccaaccacc aacaaccag atagagatg 
      gcagcggcc acaaaacgc gtcagtgat aatcgaagg 
      agcaataaa ccgatcatg gagaaacgg aggcgggca 
      aggatcaat aacagtctc aatgagctc aagacactt 
      attttggat gccatgaaa aaagacccg gcgcgccac 
      tccaagctg gaaaaagcc gacatcctc gaaatgacc 
      gtcaagcac ctccaaaac ctccaacgc cagcaagcc 
      gccatgtcg gcagcgacg gacccctcc gtcgtcagc 
      aagttcagg gccggcttc agcgaatgt gcctccgag 
      gtgggccgc ttccccggc ctggacccg gtggtgaaa 
      cggcgtttg ctgcaacac ctcgccagt tgtctgaac 
      cagggccag aaggagccc caggtccag gtgatcgtc 
      cccgaggtg gcccccaat aacattatt ctgggcaac 
      ggcaccggc gtgcaactg gtgcccacc aggttggcg 
      aacggggac attgcgctc gttttgccc acgcaggga 
      gcgtcgccg ttgcccctc ctggtgccc ataccgcag 
      aggacggcg tccacggcg tcgtcggcg tccaattat 
      tcacccagt caaagtccg gagccggag tcagtgcgg 
      ccgctttcg ctggtggtg cggaggtgc gaggagccg 
      acggaggag aagccgtgg cggccgtgg tag
    mRNA sequence
    • >TC012851 RA
      cgcccaaag ctcagcatc aacgcacac cgcgatcga 
      acgcgcttg aaatcggtc gatacttag tgatttgtt 
      gcctaccaa gatggttac gggaaccaa caacacccc 
      agtgcaacc caatgccat tccaaccac caacaacca 
      gatagagat ggcagcggc cacaaaacg cgtcagtga 
      taatcgaag gagcaataa accgatcat ggagaaacg 
      gaggcgggc aaggatcaa taacagtct caatgagct 
      caagacact tattttgga tgccatgaa aaaagaccc 
      ggcgcgcca ctccaagct ggaaaaagc cgacatcct 
      cgaaatgac cgtcaagca cctccaaaa cctccaacg 
      ccagcaagc cgccatgtc ggcagcgac ggacccctc 
      cgtcgtcag caagttcag ggccggctt cagcgaatg 
      tgcctccga ggtgggccg cttccccgg cctggaccc 
      ggtggtgaa acggcgttt gctgcaaca cctcgccag 
      ttgtctgaa ccagggcca gaaggagcc ccaggtcca 
      ggtgatcgt ccccgaggt ggcccccaa taacattat 
      tctgggcaa cggcaccgg cgtgcaact ggtgcccac 
      caggttggc gaacgggga cattgcgct cgttttgcc 
      cacgcaggg agcgtcgcc gttgcccct cctggtgcc 
      cataccgca gaggacggc gtccacggc gtcgtcggc 
      gtccaatta ttcacccag tcaaagtcc ggagccgga 
      gtcagtgcg gccgctttc gctggtggt gcggaggtg 
      cgaggagcc gacggagga gaagccgtg gcggccgtg 
      gtagaaaca gtgatgtga tgaaagtgc ttttgtgat 
      aatttgagg attgccatg ttgtatata attgtttat 
      ttttgataa agaatggtt aaattattt ataggctag 
      ggagttaag tacaagttt attactgaa aagtgcctt 
      atcaacagc aagatcatt catgtcttc cagttggta 
      atatttact atattatga atatttata ttaaattgt 
      attttattt tattatttg ctaataaaa ctgatattt 
      tcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY