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Gene information
General information
TC012848 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15644779..15646421
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012848
    iBeetle screen iB_05337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages sometimes partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012848 RA
      MPFFATEEG RYLASSLGD PLIKGLTEV ANKRPDDPI 
      AYLATYLYN FANNRQNVH SRGKTQQEN QQTITGPPQ 
      EDNNKAVPA SIEVVTVDP EDSGNEEDS AFNSTSRDE 
      HGQSMLHFA AARAHGRNA LYQLLVETE INVAYRDEL 
      YRTARDISI QANIPENTI EIDRYVMSI ATKGDTDKL 
      VELLLDGYD HITDIVDEN GVPIVEAVS QHNQPDTVS 
      FLQSILAFE EKRERVHHA IRLGSINDV SALLADENE 
      TGSGKLLAI GKNNYGRCS LHIAVLCQQ EEIADYLAN 
      TFPDTLKLG DNLERTALH YAMGVEKME TISRVLIKA 
      GARRIVKDL KGRQPTYYF LNKSDILRL QEEEETF
    CDS sequence
    • >TC012848 RA
      atgccgttt tttgcaacc gaagaaggc cgctactta 
      gcctcttcc cttggcgac cccctcatt aaaggcttg 
      accgaagtt gcaaacaaa cgccccgac gacccaatc 
      gcgtatctc gcaacctat ttatacaat tttgccaat 
      aatagacaa aacgtacac tcgagggga aaaacacaa 
      caggaaaac caacaaact ataaccggg cctccgcag 
      gaagacaac aataaggcc gttccggcc tctatcgag 
      gttgtgact gttgatccc gaagacagt gggaatgag 
      gaggattcg gctttcaac agcacaagt cgggacgaa 
      catgggcaa agtatgctt cattttgcc gcagcaagg 
      gcccatgga cgaaacgcc ctgtaccaa ttattggtc 
      gaaacggaa attaatgtt gcatataga gatgaattg 
      tacagaaca gctcgcgat atcagcatc caggccaat 
      attccggaa aacacgatc gaaatcgat cgatacgtc 
      atgagtatt gcgacaaaa ggcgacact gataagttg 
      gttgagctc ctcttggac ggttatgac cacatcacc 
      gacatagtt gacgaaaat ggggtccca attgtcgaa 
      gcagtgtcg caacacaac caaccagac actgtctcc 
      tttctccaa tcaattcta gctttcgag gaaaaacgc 
      gaaagagtc caccatgcc attcgtttg gggtcaata 
      aatgacgtc agtgcgctc ctagctgat gagaatgag 
      actggaagt ggtaaactt ttggccatt gggaaaaat 
      aactacggg aggtgttcg ctccatatt gctgtgctt 
      tgtcaacag gaggaaatt gcggattat ttggcgaat 
      acgttccct gatacgctc aaacttggc gataacttg 
      gagagaact gcgcttcat tacgccatg ggtgtggaa 
      aaaatggag acaattagt cgggttttg atcaaggct 
      ggagctagg aggattgtc aaggatctg aaagggaga 
      caaccgact tattatttt ttgaacaag tcggatatt 
      ttgaggttg caagaagag gaagagacg ttctag
    mRNA sequence
    • >TC012848 RA
      tcgatttgg tttggttcc aggtgttgt cacgtcgaa 
      ccaagtcga catgccgtt ttttgcaac cgaagaagg 
      ccgctactt agcctcttc ccttggcga ccccctcat 
      taaaggctt gaccgaagt tgcaaacaa acgccccga 
      cgacccaat cgcgtatct cgcaaccta tttatacaa 
      ttttgccaa taatagaca aaacgtaca ctcgagggg 
      aaaaacaca acaggaaaa ccaacaaac tataaccgg 
      gcctccgca ggaagacaa caataaggc cgttccggc 
      ctctatcga ggttgtgac tgttgatcc cgaagacag 
      tgggaatga ggaggattc ggctttcaa cagcacaag 
      tcgggacga acatgggca aagtatgct tcattttgc 
      cgcagcaag ggcccatgg acgaaacgc cctgtacca 
      attattggt cgaaacgga aattaatgt tgcatatag 
      agatgaatt gtacagaac agctcgcga tatcagcat 
      ccaggccaa tattccgga aaacacgat cgaaatcga 
      tcgatacgt catgagtat tgcgacaaa aggcgacac 
      tgataagtt ggttgagct cctcttgga cggttatga 
      ccacatcac cgacatagt tgacgaaaa tggggtccc 
      aattgtcga agcagtgtc gcaacacaa ccaaccaga 
      cactgtctc ctttctcca atcaattct agctttcga 
      ggaaaaacg cgaaagagt ccaccatgc cattcgttt 
      ggggtcaat aaatgacgt cagtgcgct cctagctga 
      tgagaatga gactggaag tggtaaact tttggccat 
      tgggaaaaa taactacgg gaggtgttc gctccatat 
      tgctgtgct ttgtcaaca ggaggaaat tgcggatta 
      tttggcgaa tacgttccc tgatacgct caaacttgg 
      cgataactt ggagagaac tgcgcttca ttacgccat 
      gggtgtgga aaaaatgga gacaattag tcgggtttt 
      gatcaaggc tggagctag gaggattgt caaggatct 
      gaaagggag acaaccgac ttattattt tttgaacaa 
      gtcggatat tttgaggtt gcaagaaga ggaagagac 
      gttctagga gtcatagtc catctagca aatcacttt 
      atattttta tacaccttt gtctggtct agggttagg 
      cttcagcaa tcacaaata ttttttaaa cttttttga 
      gtgttacaa ttcgaggac aaaaataaa ctatagtgt 
      gacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY